HOI NGHj KHOA HOC CONG NGHE SINH H0CTT3KIN?' •'
NGHIDN CLfU CfNG DUNG K? THUAT G A Y D O N G D U C B O N G LOAT PHUC v y
TRltN KHAI THU TINH N H A N TAO CHO BO C ^ T I N H DIEN B I E N V A T H A I B I N H Dodn Vift Binh*. Do Vdn Thu. L l Vdn Ty, Ld Thj Huf, Tran Xudn Khdi, Vo Thj NinhVi^n Cong ngh$ sinh hgc, ViSn Han lam Khoa hgc vk Cdng nghg Vidt Nam
T 6 M TAT
Thv tinh iMn t ^ cho gia sue giAp tang nhanh ti€n bq di truyen v^ cai tien gidng nhSm nang cao nang suat, chat lirong ciia gia sAc. O nude ta, cho d&i nay, thu tinh nhan t?o rSt khd triin khai do da s6 bo duyc nuoi d quy mo nhd le nen thudng dgng due rii rac, phan lan. B^i hia aky triidi bay v i ^ sii: dung gay dgng dye ddng lo^t cho bo de tao dieu ki€n tbu|in Igi cho v i ^ tnen khai phuong phip thy tinh nhan tao nhim nnic dich nang cao nang suat va chit lugng dan bd t^i hai linh Di?n Bien va Thii Binh. Tong so c6 738 bd dugc liem theo 5 cong thiic khic nhau. Cong thuc 1: Tiem 2 mvii PGF2a each nhau 11 ngiy, kat hgp tiem PMSG vdi miii tiem OuSr hai.
Cdng thirc 2: TiSm 2 miii PGF2a each nhau 7 ngiy, kit hgp tifim HCG. Tiem 2 mui PGF2a each nhau 11 ngay, kit hgp tiem FSH.
Cdng thiic 4; Tiem GnRH, sau 7 ngay tiem PGF2a, sau 2 ngay tiip theo tiem nhic lai GnRH. Cdng thirc 5: Tiem GnRH, sau 7 ngay tiem PGF20. Ket qua cho diiy bd ti&n cdng thiic I dat t^ 1§ d^ng dye vi thv thai 1^ 90 va 68,5 % (d Di?n Bi6n), 84.9 vfi 82,88 % (cr Thdi Binh). TiSm flieo cdng thiic 2,3,4,5 dat ty 1? dgng due li 57,1,80,8,62,82,08 % vi ty 16 thu thai la 75,81,69,4,80,28 %. GSy ddng dye doQg loat kgt hgp thu tinh nhin tao li phuong phip co th8 ap d^ng dl giiip nang cao nang su^t vi chit lugng din bd vi cong thiic I li quy trinh gSy dgng due cho bd phii hap vdi thirc liln a Vi?t nam.
Til khoa: Bd, ddng due dong lo^t, thv tinh nhan t^o.
MdGAU
Thg tinh nhdn tgo (TTNT) cho gia sue giup tdng nhanh tiSn bd di tmydn vd cdi tiSn gi6ng nhdm ndng cao ndng suSt, chit lugrng cilia gia sdc. O niritc ta, cho den nay, TTNT mdi chf du'Q'c th|,rc hidn d vung ven cua mpt so tinh thanh idn, nai cd nhOng di^u ki$n thudn Igri d l thifc hifn ky thudt ndy. Do dd, ddn bd lai mdi dgt du^c t9 1$ 28% trong tong dan bd (Vang,2003). MOt trong nhOng nguydn nhan Idm chdm sy phdt trien cOa ddn bd lai Id do 6- Vidt Nam cd r&t (t frang fr^i nudi bd thjt tdp tmng vdi s6 luvng Idn, da so bd du'p'c nudi d cdc hg gia dinh cd quy md nhd le, phdn tdn. Bd cua cac hd ddn thu'dng ddng dgc rdi rdc quanh ndm, ddng diic chdm sau khi sinh con hodc ttidm chi khong dgng dgc. Thg tinh nhan tgo rit khd cd the triin khai do cdc din tinh vien phai dau ttr thdm rElt nhieu cho clii phi mua ni to' Idng d l bdo qudn tinh, kdo theo tdng tong chi phi cua TTNT vd gidm hi^u qud kinh t l . Do dd, rSt can phai cd gidi phdp ky thugt giiip cho TTNT cd thi trien khai rdng rai tgi khap cdc ^ a phu'O'ng trong cd nud'c.
Gdy dpng dgc ddng logt (DDDL) Id mdt ky thudt sinh san tidn tiln vO-a cd t h i gJOp chii dOng dieu khiin thd'i gian ddng dgc cua mdt sd lutrng Idn gia sOc tdp hung trong khodng thdi gian nhit djnh Igi cd the giup dilu tii nhQng gia sue cd cdc b§nh sinh sdn do r^i logn ngi tiet. Cho den nay, c6 rat nhieu quy trinh gdy DDDL dugrc xay dg-ng trdn ca sd si> dgng dcrn Id hay phoi hgp nhilu iogi hormone. Tdn cCia cdc quy trinh du-cc dgt theo each thu-c si> dgng cdc hormone Ok gay dpng dye, bao gim: Quy frinh tidm Prostaglandin F2a (PGF2a), quy trinh chpn lpc {Select Synch), quy trinh kit hgp (CO- Synch), quy frinh laj (Hybrid Synch) (Stevenson ef a/„ 2000). Cae quy trinh nay da dugc dp dgng thanh cdng cho cd bd cdi tg {Schmitt ef al., 1996) vd bd cdi sinh san (Larson et al., 2006), ca bd s&a (Puriey ef al., 1995; Wolfenson ef al., 1994) iSn bd thjt (Amjad etal:, 2006; Stevenson efaf., 2000; Taponen, 2009; Twagiramungu efaA, 1992), cd bd c6 chu ky hay khdng cd chu kjr sau khi de {Berardinelli et al., 2007; Larson ef ai., 2006; Stevenson ef al.. 2000).
Trong bdi bdo ndy, chung tdi frinh bdy si> dyng gdy DDDL cho bd thjt d l tao dieu kign thudn Igi cho viec triin khai phugng phap TTNT nhim muc dich ndng cao ndng suit vd chit luang ddn bd tgi mdt s6 huygn cua hai tinh Didn Bidn vd Thdi Binh.
VAT LI^U VA PHlfOYJG PHAP NGHIEN CO'U V|t lidu
Prosoivin (PGF2a), Buserelin (GnRH), Conulon (HCG) (cua hang Intereet), PMSG (pregnant mare's serum gonadolnspin) (Tmng^tdm Cdng nghg sinh hpc Ddng nam A Vidt Nam), Folltropin(FSH) (eua hang Bioniche). Tinh dQng Ignh cpng ra cua 2 giong bd: Red Sindhi va Brahman cua Trung tdm Giing gia sue Idn Tnjng irang.
Bd cdi thudc giong bd vang cua dja phuong va bd lai Sind, a i khoi lugng > 200 kg.
Phuvng phdp
Ngay trudc khi tiem ttiudc, bo Oirgc khdm qua frye trang, kiem fra frang thdi tir cung, hogt dOng cOa bu6ng fri>ng. Sau dd bd dupe xip ngSu nhidn vao cdc Id d l tiem hormone theo cac cdng thiJc sau day:
Cdng thu'c 1: PGF2a - PGF2a + PMSG; cdch tidm: ngay 1 tiem 2 ml PGF2a - sau 11 ngdy tiem nhac Igi 2 mi PGF2a kit hgp vdi 500 IU PMSG . Tiem vdo ca mdng eua bd.
Cdng thu'c 2 : PGF2a - PGF2a + HCG; each tiem: ngdy 1 tiem 2 ml PGF2Q - sau 7 ngdy tiem nhIc Igi 2 ml PGF2a kit hgp vdi tidm 1000 IU HCG . Tidm vao ca mong cda bd.
Cdng thu'c 3 : PGF2a - PGF2a + FSH; each tidm: ngay 1 tiem tidm 100 pg GnRH - sau 7 ngdy tiem 2 ml PGF2a kit hw vdi tiem 12mg FSH. Tiem vao ca mdng bd.
HQC CONG NGHE SINH HOC TOAN QUOC 2013
Cdng thuc 4 (quy Irtnh k^ hgp): GnRH - PGF2a +GnRH ; cdch tiem: ngay 1 tiem fldm 100 pg GnRH - sau 7 ngdy tidm 2 ml PGF2a, sau dd 48 g i * tidm nhde Igi 100 pg GnRH. Tiem vao eg mdng bd.
Cdng thuc 5 (Quy trinh chpn lpc): GnRH - PGF2a; cdch tifim: ngdy 1 tiem 100 ug GnRH - sau 7 ngdy tiem 2 ml PGF2a.
Tiem vao ca mdng bd.
Bd cua finh Didn Bien dugc tiem frieo cdc cdng ttiirc 1,2,3,4. Bo cda finh Thdi Binh dugc tidm tiieo edng thOrc 1 vd 5 Sau khi tidm hormone nhir tren, bd cdi dugc theo dot dpng dgc vd nhirng bd cd bieu hien dgng due dugc thg tinh theo phuang phdp din tinh kdp vdo budi sang vd chieu. Tinh cgng ra sir dgng Id logi 0,25 ml/1 lln. Dung sung bin tinh d l din tinh cho bd.
Xdc djnh ty le Uig thai cda tid bang phucmg phdp khdm thai qua true frang, dugc thgc h i ^ sau 2,5-3 thdng sau khi phii gilng cho bd.
Cdc kit qua thu dugc dugc xd ly theo phugng phdp xac xuit thdng ke bdng phan mem Excel.
K ^ QUA vA T H A O L U A N
ThM gian nghi (tip khi de d i n khi khdm) cua ddn bd duoc xir ly gay ddng due
Cd tong s6 214 bd cua Oi0n Bidn vd 524 bd eOa Thdi Binh dup'e kham vd dua vdo ttil nghidm. Kit qui cho ttily, a cd hai tinh, s6 bd c6 ttidi gian tir khi dd din khi khdm trong khodng 3-4 Ihdng chilm ty Id cao nhit (xem bdng 1).
B i n g l
"Hnh Di$n Bi&i ThSi Binh
Thih gian ngh S6 bd ktidm(con) 214 524
ciia ddn bd du^gc gSy d$ng dgc dong logt dugc < 1 thdng
S6con 21 83
T y i d ( % ) 9,81 15.83
1-2 thang So con
49 131
Ty i§ {%) 22.89
25 3-4 thang S6con
94 191
Ty le {%) 43.92 36.45
> 4 t h i n g s 6 c o n
50 119
T y l $ { % ) 23.36 22,7 Kit qua gay dgng dgc d6ng loat cho bd
Cd tong s i 738 bd eua hai tinh Di|n Bien va Thdi BInh dugc tiem tiieo cdc quy frinh khdc nhau de gdy dpng dgc. Kit qud cda mdi quy frinh dugc ttiuc hign a timg dja phuong dugc frinh bay d bang 2 vd bang 3. Theo dd bd dugc tidm ttieo cdng ttiire 1 dr cd hai tinh diu dgt tJ Id dOng dgc eao nhit trong khi bd dugc tidm theo cdng thuc 2 cd t} 1$ ddng dgc thip nhit. Da s i bd sau xir l<^ honnone cd bilu hidn ddng dgc gilng nhu dgng dgc tg nhien. Mdt s i bd tidm PMSG frong cdng ttiirc 1 tiet djch nhilu, dpng dgc dai ngdy (tdi 5 ngdy). Mpt so bd khac ddng dgc khdng mdnh Ijgt, dgc bidt djeh tilt dm dgo it hgn txi d$ng dgc tg' nhidn.
Bing 2. Ti l | d^ng dgc cua bd sau khi tiim hormone tfil tinh Oi$n Bien COng Ihuc 1: PGF2a - PGF2a
+ PMSG B6 ddng dgc/Bd b i m (con)
54/60
TJ I f ddng dgc (%)
90
Cdng ttiiic 2: PGF2a - PGF2a + HCG
Bd ddng dgc/Bd t i i m (con)
Ty 1$ dgng dgc (%) 28/49 \ 57,1
Cong thiic 3 PGF2a - PGF2a + FSH
Bo dgng duc/B6 tiem {con)
Ty 1$ ddng dgc (%) 21/26 1 80,8
Cdng ttiiic 4: GnRH - PGF2a + GnRH Bd ddng dgc/Bd bdm (con)
49/79 Ty 1$ dong dgc (%j
62 Bing 3. TJ If dong dgc ciia b6 sau khi Uem hormone t^l tinh Thil Binh
Cdng thuc 1. PGF2a - PGF2a + PMSG Bo dgng dgc/Bo tiem (con) 298/351
Kdt qud thi^ tinh n h a n t a o c h
Ty l§ dgng dgc {%) 84,90 3 b d
Cong thiic 5. GnRH - PGF2a Bo dgng duc/B6 liSm (con)
142/173
Ty \e ddng dgc {%) 82,08
Tit ca s i bd sau tidm hormone sinh san phdt hidn cd bilu hidn ddng dgc da duge thg tinh nhdn tgo. Kit qua thg tinh nhdn tgo cho bd d Dign BiSn vd Thdi Binh duge frinh bay a bang 4 vd 5. Kit qua cho ttily bd tidm hormone theo cdng ttiirc 1 tgi Thdi Binh cd ty 1$ thg thai cao nhit, dat 82,88%. T^ lg bd thg thai d tinh Dign Bien nhin chung dgt frilp han d finh Thdi Binh.
Bdng 4. Ty 1$ thu thai c u a b d sau khi gSy dgng dgc ddng lo^t tg Cdng ttiUc 1: PGF2a - PGF2a
t P M S G Odng dgc/thg thai (con)
37/54
TJ 10 thg thai
(%)
68,5
COng tiiiic 2: PGF2o - PGF2a + HC6
O^ng dgc/thg thai (con)
21/28
Ty 1$ thg thai
(%)
75
Dien Bien
Cong thiic 3: PGF2a - PGF2a + FSH
D$ng dgc/thu thai (con)
17/21
TJ 1$ thg thai
(%)
81
Cdng Uiiic 4: GnRH - PGF2a + GnRH
Bdng dgc/thg thai (con)
34/49
Ty 1$ thg thai
(%)
69,4 g 5. Ty I f thg thai ciia bd sau khi g i y ddng dgc ddng loat tai Thai Binh
-Ft
Cdng ttiirc 1: PGF2a - PGF2a + PMSG Ddng dgc/thu thai (con)
247/298
Tyl§ thg thai (%) 62,88
Cdng thirc 5: GnRH - PGF2a Bdng dgc/thg thai (con)
114/142
T y l d * g t h a i ( % ) 80,26 THAO LUAN
Co rat nhilu quy trinh gay DDDL duge xdy dvng frdn ca sd- su dgng phii hgp nhieu Togi hormone. Vigc l^ra chpn dp dgng quy frinh thfch hgp phg thudc vdo dilu kidn thg'C te vd mgc dich cua ngudi thg'C hidn. Kit qud kilm tra ddn bd cua hai ti'nh Di§n B i ^ vd Thdi Binh cho ttily, s6 bd ed ttidi gian nghi sau khi de Icm hgn 3 thdng chilm tJ Id rat cao ( 67.28 d Dign Bien va 59,15% d Thdi Binh ). Dilu dd cho thiy sire sinh c^a ddn t)d kdm vd treing dan bd cd nhieu con da dpng dgc sau kht dd vd cd cd nhOng con khdng ddng dgc. Can cir fren thg'C t l , chOng tdi da chgn thu> nghidm hai quy trinh gay DDDL cho dan bd thjt c6 vd khdng cd ehu kJ sau khi de.
Vdi edng thiic 1, PGF2a duge tiem hai Idn, cdch nhau 11 ngdy. Tidm PGF2a cho bd vdo phan Idn tiidi gian trong chu kJ sinh dgc diu gdy tiiodi hda t h i vang, Idm gidm mO'c progesterone trong mdu vd tgo dieu kign cho nang trirng m6>i phdt triin kich ttilch bd ddng dgc {Henricks ef a/., 1978). Do bd cd nhieu sdng nang to^ng mdt chu kJ (Roche ef a/., 1991) ndn tiem PGF2o hai tdn se giOp Idm gia tang ty lg d$ng dgc. Quy trinh hai lan tidm PGF2a each nhau 11 ngdy da dugc nhilu tdc gid dp dgng gdy dong dgc ding loat cho bd {Stevenson ef al., 2000 ). ChOng tdi da thir nghidm tidm them PMSG kit hgp vdi mui tidm PGF2a thi> hai nham kfch thfch nang tn>ng phdt friln. Kit qud cho thdy s6 bd ddng dgc dgt t^ Id rit cao {84,9% d Thdi Binh vd 90% d Dign Bien), eao han kit qud thu dugc ciia Stevention e( at.. 2000. Tuy nhidn, nhugc diim cua phuang phdp ndy la ed mpt so bd cd thdi gian dgng dgc bj keo ddi Idn din 5 ngdy. Trong tnrd'ng hgp ndy ddi hdi din tinh vidn phdi Id ngudi cd kinh nghidm theo ddi cac bilu hign dOng dgc vd ddi khi phdi tdng lilu tiig tinh nhilu han so vdl binh ttiudng ( > 2 lln ddn tinh). Do vdy, tJ Id bd thg that trong quy trinh ndy dgt dugc a Dign Bidn Id 68,5%.
frong khi d Thdi Binh ty le ndy rat cao (82,88%), cao han so vdi ty Id dgt dugc (52,2-55,8%) cua Lauderdale et al. (1974) chl tidm mdt mui PGF2a hay cua Stevention ef at.. (2000) sau khi tidm 2 mui PGF2a eho bd thit cd diu kJ (70,6%).
So sdnh kit qud xir 1;? gdy ddng dgc bd vdi cdng thirc PG-PG+PIWSG vd PG-PG+HCG eho thiy tJ 1$ bd dgng dgc d quy trinh sau thdp hgn bin. Trong trudng hgp dung GnRH-PG kit hgp vdi FSH, ty Id ddng dgc vd ty 1$ thg thai deu dat tuang doi cao (80,8 vd 81,0%). Dgt duge dilu ndy Id do kfch thfch phdt triin nang, FSH kfch thich phdt tiiln nang nhung khdng gdy ddng dgc kdo ddi, ndn din tinh thudng dugc dung thd'i diem. Tuy nhidn do FSH cd gid Uidnh cao, phdi bao qudn ddng lanh khi da pha. Igi hiem tren thj trudng ndn quy frlnh ndy it dugc pho biln.
Quy trinh xir li vdi GnRH (quy tiinh ovsynch), tidm thdm mgt mOi GnRH 48 gid- sau khi tidm mui PG, Puriey ef al., {1995), cho thdy cd thi ktidng can ttieo ddi ddng dgc, dan tinh 12-16 gid sau Wii tidm GnRH eho kit qud thg thai 46-55%
d ddn bd sua . Ddi vdi bd thjt, trdn ddn I6n. edc gilng khdc nhau, lira dd khdc nhau, xir li bd vd'i GnRH vd PGF2a, Twagiramungu ef al.. (1992), cho tJ 1$ ddng dgc dgt 83,3% vd ty id thg ttiai cung dgt 70.8 % . Ty Id thg thai ed t h i cao han niu xir ly homione kit hgp vdi vide tdch be khdi mg. Nghidn cu'u cCia chung tdi eho thiy thg thai dgt tdi 69,4% trdn sd bd ddng dgc sau GnRH-PG+GnRH. Cf cdng ttiipc 5. GnRH dupe tiem trudc r i i sau dd 7 ngay bd dugc tidm PGF2a.
Lgi t h i ciia vide tidm GnRH rii sau dd tidm PGF2a d ch6 tidm GnRH gdy n,ing tn>ng hoge gdy hodng thi hoa cde nang ddc tdn, hlnh thdnh mOt sdng nang mdi vd tCr sdng nang mdi ndy mgt nang phdt triin thdnh nang dgc tdn. Bdi nang dOc tdn hinh thdnh tixjng vdng 8 ngay vd qud frinh ddc tdn nang keo dai 4 ngdy nu-a (Roche ef al., 1991), tilm PGF2a vdo ngdy thir 6-7 sau GnRH gdy tiiodi hda t h i vdng cung id iOc nang dpc tdn d giai doan egn n,ing tn>ng se cho n,ing tnJng.
Xir li nhu vdy se lam gidm giao dgng khoang tiidi gian din tiidi diim dOng due d' tid sCra (Wolfenson ef al.. 1994 ) vd d bd cdi tg (Schmitt ef al.. 1996) Idm cho khoang tiidi gian tO' khi tidm PGF2a din ddng dgc Id 3-5 ngdy. Bd dugc lidm theo cdng ttiuc ndy cd tJ lg ddng dgc (82,08%) thip hgn so vdi edng thirc 1, nhung sy khdc nhau ndy khdng ed J nghTa thing kd. Bd tidm ttieo cdng thuc ndy niu dugc kdt hgp ttieo ddi dpng dgc chgt chd tuy ed mit nhilu tiidi gian han cho vide theo ddi bd ddng dgc d l TTNT song tJ le bd thg thai Igi dat dugc rit cao (80,28%).
Vdi ket qua nh^dn Oaqfc, chung tdi nhdn d|nh cd 5 cdng thO'C hormone sinh sdn dua vdo u-ng dgng d l gay ddng dgc ding logt diu cho ket qud tit. Vdi ttiuc t l , dan bd cd thdi gian nghf sau dd hgn ba thdng cao nhu v§y neu dai bd dpng dgc tv nhidn rdi mdi TTNT thi so bd dpng dgc va thg thai se kdm han rat nhilu. Vi vgy, gay DDDL kit hgp vdi TTNT se Id mdt gidi phdp hO'U hidu de giOp ndng cao nang suit vd chit lugng ddn bd. Tuy nhidn, ndn sir dgng cdng thirc 1(PGF2a - PGF2a + PMSG) d l gdy ddng dgc cho bd, vi hai ioai honnone PGF2a va PMSG cd sin d thj tnrdng cOa Vidt Nam gii tiidnh rd han cdc logi hormone khdc.
K£T LUAN
Kit qud gdy DDDL fren bd vdng i^a phuang vd bd iai sind kit hgp TTNT cho thdy: Quy trinh tiem bd 2 mui PGF2a cdch nhau 11 ngdy kit hgp tidm PMSG d mui tidm ttiir hai tgi hai finh Thdi Binh vd Dign Bidn cho ty Id ddng dgc la 84 90 v^
90%. ty Id thg tiiat id 82.88 vd 68,5 %. Tgi tinh Dien Bien. quy trinh tidm bd 2 mui PGF2a each nhau 7 ngay kit hpp tidm HCG dgt ty lg dOng dgc va tJ lg Uig ttiai Id 57,1 va 75%; quy trinh tiem 2 mui PGF2a cdch nhau 7 ngdy kit hgp tigm FSH dgt ty Id dgng dgc va tJ lg tiig ttiai Id 80,8 vd 81%; quy frinh tidm GnRH sau 7 ngdy tidm PGF2a vd 48 gid sau PGF2o tidm nhac ^'GnRH eho tJ lg dOng dgc ia 62 %, tJ Id thg thai Id 69.4 %. Tgi finh Thai Binh. quy trinh tidm GnRH sau 7
?Sl ! r £ ^ '^ ^^"^ '^^^ '^ ^ ^ ' ° ^ "''''• *^ '^ t^'y ^^' 1^ S°'28 %• Gdy DDDL kit hgp TTNT Id phugng phap cd ttie ap dgng dd giup nang cao nang suit va chat lugng dan bd vd cdng tiii>c 1(PGF2a - PGF2a + PMSG) la quy trinh gdy ddng dgc cho bd phii hgp vdi thg-c tidn d Vigt nam.
TAJ LI$U THAM KHAO
^^l?,**,^' ^'^^"^ ^' ^^^^'^ ^'^ f ^ ° ^ ' - ^^^ °f prostaglandin (PGF2a) to induce oestnis in postpartum Sahiwal cows. Pakistan Vet J., 20(2)' 63-66
Berardinelli JG, PS.Joehi and SA Tauck (2007). Conception rates to artifkaal insemination in primipanous, suckled cows exposed to the btostimulalory effect of txiils before and diaing a gonadotropin-releasing tiomnone-based estrus synchronization protocol. J. Arum Sd 85: B48-e5Z
HenricksDM, JR Hill (1978). Effects of PMSG and PGF2a on gonadal hormones and reproductiOTi in Uie beef heifer Joumal of animal sdence, 46(5}-1309-1315.
Lauderdale JW. Segun BE, Stellftig GN. Chenault GR. Thatcher WW. Vmcenl OK. Loyancano AF (1974). Fertlity of catties following PGF2a injectmn. JAnhn Sd. 38:964-967.
Larson JE, GC Lamb. JS Stevenson. SK Johnson, ML Day. TW G e » y , DJ Kesler, JM DeJamelte, FN Schnck. A DiCoslanzo and JD Arseneau (2006). SynchrcKiization of estrus in suckled tieef cows for detected estnjs and artificial insemination using gonadotropin- releasing homione, prostaglandin F2a and progesterone. J. Anim. Sd., 84. 332-342.
n dairy cows using PGF2a and GnRH. Theriogent^ogy. 44- Rodne JF, Boland MP (1991). Turnover of dominant follicles In catile in different reproductive status. Theriogenotogy. 35:81-90 Schmitt EJP, Diaz T, Drost M, Fredrksson EW, Thatcher WW (1996). Use of gonadotrpin-releasing homione or human chorionic gonadotropin for timed insemination in cattle. J Anim Sa, 74:1084-1091.
Stevenson JS, KE, Thompson W, L Fod>es, GC Lamb, DM Grieger and LR Corah (2000). Synchronizing estius and (or) ovulation In beef cows alter combinations of GnRH, nOTgeslomet and prostaglandin F2alpha witti or witiiout timed insemination J. Anim. Sd.. 78- 1747-1758
Taponen J (2009). Fixed-time artiiicial insemination in beef cattle. Ada Veterinaria Scandinavica, 51(48). 1-6.
Twagiramungu H, Guitbauit LA, Proulx J, Vilteneuve P, Dufour JJ (1992). Influence of an agonist of gonadotropin releasing (buserelin) on estrus synchronization and fertility in tieef cows. JAnimSd, 70:1904-1910.
Vang ND (2003). The Viebiam National Country report on Animal Genetic Resources. Nxb Nong nghidp. 1-103.
Wolfenson D, Thatcher WW, Savio JD, Badinga L, Lucy MC (1994). The effect of a GnRH analogue on tiie dynamics of follicular development and synchronization of estrus in lactatlng cyclic dairy cows. Ttieriogenology, 42:633-644.
APPLICATION OF ESTRUS SYNCHRONIZATION FOR ARTIFICIAL INSEMINATION OF BEEF COWS IN DIEN BIEN AND THAI BINH PROVINCES
D o a n V i e t Binh*, D o V a n T h u , Le V a n Ty, L e T h i H u e , Tran D a n g K h o i , V o T h i Ninh Institute of Biotechndogy. VAST
SUMMARY
Local yellow cattle breed still made up a high percentage of total catUe population in Vietnam. These breed should be inseminated with exotic breed to increase their genetic ment. As the catde bred in smallholder fermer^ display estrus sporadic and uncoacentrated, s^lication of artificial insemination (AI) tends to limit to areas in close proxinuty to urban aty. Therefore, solutions should l>e fiiiind to enhance the possibilities for using artificial insemination. This paper reports the application of estrus synchronization for artificial insemination of beef cattle in Dien Bien and Thai Binh provinces, where die rate of artificial insemination is still very low. A total of 738 local yellow and Sind crossbreed cattle (214 cattle in Dien Bien and 524 cattle in Thai Binh province) were assigned randomly into one of five experimental groups. Group 1, in which cows received 2 ml of PGF2a, followed in 11 d witti 2 ml PGF2a and 500 UI PMSG on the same day. Group 2 and 3 includes cows received 1000 IU HCG or 12nig FSH instead of PMSG as in the groupl. Group 4, m which cows were administered lOOpg GnRH, followed in 7 d with 2ml of PGF2a At 48 h after PGF2a, a second GnRH was administered. Group 5 contains cows were injected GnRH and PGF2a as group 4 but not repeated GnRH mjections as group 4. After the last injection in each group, cows were observed for estrus twice daily and those displaying estrus were artificial inseminated. At the end of experiment, rate of detected estrus in Che group I is 90 % in Dien Bien, 84.90 % in Thai Binh and rate of conception achieved 68,5 %in Dien Bien and 82,88 % in Thai Binh. The rates of eslnis and conception ID the group 2 are 57,1 % and 75% while those of group 3 are 80,8 % and 81%; group 4 are 62 and 69,4% together with the results of group 5 are 82,0S and 80,28%, respectively. The results showed that synchronization of estrus has the potential to enhance the possibilities for usmg AI and to help improving productivity and quality of beef cattle
Keywords: Artificial insemination, cow, estms synchronisation.
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