HOA HOC-CONG NGHE TH!fC?HAM
KHAO SAT KHA NANG
KHANG OXY HOA CUA DICH CHIET Tl/
VO QUA THANH LONG R U O T DO (HYLOCEREUS POLYRHIZUS)
• HUYNH TH! HONG HOA - NGUYEN XUAN THj DIEM TRINH - LE TH! KIM NGAN - T R U O N G TH! NGOC THUY
T 6 M TAT:
Bai vie't nghian ctfu v^ kha nSng chi6't tach cung nhu" Ichang oxy hoa cira dich chie't iCf v6 qud thanh long ruOt d6 bang phi/dng phdp DPPH. Khao sat dUdc tie'n hanh vdi cdc dieu kien ngam chie'i dung m6i nhiTsau: nhiet do say nguyen lieu thich hop 60°C, thcfi gian sa'y la 30 phut, ty le dung moi/
nguyen lidu \h 25/10 (v/w), ty le dung moi ethanol/ nu'dc thich hdp la 60/40 (v/v), th5i gian ng3m chi£'t4gi&, nhi6t dO ngam chie't 5 nhiet dp phong, tie'n hanh ngam chie't I lan, pH 4 - 6 lam dich chifi't on dinh. Ke't qua cho tha'y, dich chiel cd kha nang khang oxy hoa, do gidm ha'p thu Id 46%
vdi n6ng d6 theo trolox la 405|iM trolox/lit dich chiS't (^irnol TEAC/L).
TiJf khoa: Dich chie't, vd, thanh long mot dd, khang oxy hda.
l.D§tva'nd^
Trdi thanh long rupt dd (Hylocereus polyrbizus) chu'a sdc to cho'ng oxy hda betacyanin. thuoc nhdm sdc to' ii; nhien betalain, lan trong mfdc, diTdc iim Ihay trong hoa, trdi. Id va cu cua nhieu loat thu'c vat khde nhau nhd: hoa xtfdng rong, vd thanh long, hoa giay, rau va cii den,.. [1]. Do cd mau sac sac s3 va phong phii. sac lo' betacyanin dtfdc stf dung lam chat tao mau trong cong nghiep ihtfc pham. Ngoai lac dung la cha'l mau thien nhien dtfdc stf dung an to^n trong thtfc pham, betacyanin cdn la cac hdp chat polyphenol cd boat tinh chd'ng oxy hoa, phdng ngilfa va lam giam nguy cd benh tim mach, lieu
<lifftng, viem khdp va ung thtf... Nh& nhtfng dac tinh sinh hoc quy bau dd nen betacyanin dtfdc tfng dung nhilu trong dtfdc pham. So vdi da so' cdc chat mau thien nhien, betacyanin la cha't mau cd dp ben kem hdn, n6 chi the hien tinh ben trong mdi trtfdng acid.
Bp ben ciia cac betacyanin phu thuoc vao nhieu
>eu i6 nhtf: dp pH, nhiet dp. ion kim loai. ox\. anh
^ng. enzyme [2].
Theo "Nghien cdu tren ihdnh phdn linh chd'i bda ly ciia vd trdi ihanh tcmg (liylocerreusi polyrhizus)" cua }am'[\ah va cong stf (2011) |3]
cho ke't qua vd chtfa ham kfcfng cao cac sdc to' betacyanin (150.46 ± 2.19 mg/100 g) va peclin 10.8%.
Hien nay, Bmh Thuan dang xay dtfng mdi dtf an san xual thanh long ddng hop xua't khau vdi cdng suat khoang 7.000 ta'n/nam. Khi day chuyen di vao hoat dong se thai ra mot khoi Itfdng ddng ke vd qua Ihanh long (tren 2.000 lan/ndm) [ 1 ], dong nghia vdi viec thai bd mot nguon ldn chat cho'ng oxy hda.
Dieu nay khdng chi gay 6 nhi§m moi irtfdng ma cdn la stf lang phi v'l vd thanh long cd chtfa cha'l cho'ng ox\ hda ttf nbie n quy gia.
Nghien ctfu dtfa ra quy irinh chie't tach djch chie't ttf vd thanh long rudt dd (Hylocereus polyrhizus) bdng phtfdng phdp ngam chiel va khdo sat kha nang khdng oxy hda cua tlich chiel bdng phtfdng phdp DPPH (l.l-Diphenyl-2- picryihydrazyl).
TAPCIICgiUTIIirilllG
2. Do'i ttf<?np vil phtfcfng phap nghien cihi 2.1. Do'i tUOng nghien cihi
\ghicn ctfu stf dung nguyen lieu dims de ngam chiet la \d qua thanh long rudt dd (Hylocereus polyrhizus). Qua ihanh long rudt dd (so' Itfdng 20 kg. chpn nhtfng qua vtfa chin, vd cd mau dd hdng dam) dtfdc thu mua d vu'dn a'p Thifen Chanh. \a Phu'dng Thanh, huyen Cang Long, tinh Tni \inh.
NL:ii>cn lieu sau dd du'de sd che va bao quan lanh dcn khi stf dung.
2.2. Phififngphdp nghien cdu
2.2.1. Xdc djnh ham luang dm Irong nguyen Heu:
Hdm iif0ig dill nguyen lieu difgc .xdc dinb theo Difgc dien \'i('t Nam, iheo phuang phdp sd'y.[4]
2.2 2. Xdc djnh dien kien thich help tien hdnh ngdm chiel:
Vd qua thanh long itfdi dtfdc ciit thanh mieng nhd (2 - 3 mm), sau dd trdn deu, can chinh xac Ihiinh nhieu mau nhd roi cho vao tiii, bit kin. bao quan lanh den khi chiei.
Khdi IiTdng nguyen lieu vd qua thanh long rupl dd cho moi lan thi nghiem la 40g. Thi nghiem bd' tri ngau nhien \ di I hodc 2 nhan id' thay doi. cdc nhan Id' cdn lai cd dinh trong sud'l qud trinh thi nghiem.
Lifa chpn cac thdng so' td'i tfu dtfa vao dd ha'p ihu
ctfc dai dtfdc xac dinh bhng md> quang pho UV-Vis... Ket qua to'i tfU ciia thi nghiem irtfdc dilng lam cd sd cho thi nghiem sau.
De xdc dinh dieu kien ihlch hop chie't tdch djch chiet vd thanh long bhng phtfdng phap ngam chiel, tie'n hdnh cdc thi nghiem nhu'sddd 1.
2.2.3 Khdo sdt khd ndng khdng o.xy bda cua dich chiet bdng pbucJng phdp DPPH
DPPH (1.1-diphenyl- 2-picrylhydrazyl) la hdp chd'l cd miiu tini. dtfdc phal hien ct btfdc sdng 517 nm. Klii cac dien ttf le cua cac gd'c ttf do DPPH k6'l hdp vdi hydro ttf cha'l chd'ng oxy hda se hinh thdnh nen DPPH-H. luc nay mau sac chuyen ttf mau tim sang mdu vang. Stf bie'n doi mdu nay ttfdng tfng vdi Itfdng electron ket hdp vdi DPPH. Do dd, kha ndng lam sach gd'c ttf do cua mdt chat cdng cao lhi stf hd'p thu quang pho dtfdc do d 517 nm c^a phdn tfng DPPH cd gia tri cang giam va ngtfdc lai.[5]
Trolox (6-hydroxy-2,5,7,8-tetramethylchroman- 2-carboxylic acid. Vitamin E) la cha't chd'ng oxy hda difdc cung ca'p bdi Hoffman-La Roche, thtfdng dtfdc stf dung de so sanh kha nang tam sach gd'c ttf do cua cac chat can dtfdc khao sdt. [5]
Li/dng mdu can thie't de phan tfng vdi mdt ntfa Itfdng DPPH (hay dp hap thu 50%) dtfdc gpi Id
Sd dd 1: Khao sat diSu kien thich hpp ngam djch chie't vo thanh long
^ •-('Nhiet do phdng Say (40'>C), Say (60°C) _
<
Nhiel do say288 So 7 - Thang 4/2020
KdAOqC-CONGNGHE THirc PHAM
hftjng ttfdng doi Trolox phdn tfng. Khi dd, hoat linh ch^ng oxi hda cua mSu co the dien ta bdng thuat ngi? la so micromole bang Trolox/lOOg mau, hay ddn vj Trolox/lOOg, hay TE/lOOg. (TE: trolox equivalent).
DifSng chuan DPPH dtfdc tinh theo pM Trolox iheo phtfdng uinh dtfdng chu^n Id y = 0,1114x + 0.8425 (R= = 0,993).
Phdn u'ch boat linh chong oxy hda DPPH (2.
2-Diphenyl-1 -picrylhydrazy)
Ket qud hoal tinh khdng oxy hda du'de bieu diln bSng gia tn EC50 (Effective Concentration of 50%) difdc tinh dtfa tren phifdng trinh tuye'n tinh ciia dich chiet khao sat.
Chud'n bi thuo'e Ihtf:
Hda tan DPPH trong methanol tdi nong dp 0,15 mM (dung dich A);
Pha loang dung dich A bang methanol cho idi Jat dp hap thu 1,1 ± 0,02 d 517nni (dung dich B).
Dung dich B dtfdc chuan bj mdi cho mdi phep thtf.
Dtfng dtfdng chuan trolox IOO; 200; 300; 400;
500; 600,700 pM.
Kha ndng bdt cdc go'c ttf do DPPH cua mdu tlitf (%) ttnh bhng cdng thu'c:[4]
%D0 gidm hd'p Ihu = [ U :^™?!U!k]x 1D0[2.1]
Trongdd: \amp\e '^ do hd'p thu cija mdu pha vdi DPPH; A^.„„,^„| la dp ha'p thu cua DPPH khdng cd pha mdu (dung dich B).
3. Ket qua va thao luan
3.7. Hdm luang dm trong vo qud thanh long Vd qua thanh long chtfa ham Itfdng nu'dc khd cao (49.79%), do dd rd't dl bi htf thd'i. VI the, v6 sau khi bdc Idch nen tien hanh sa'y va ngam chie't ngay de trdnh dnh htfdng de'n qua trinh chiet.
3.2. Quy trinh dieu che dich chiet tii vo qua thanh long
Ket qud khdo sat cdc dieu kien anh hifdng den quy trinh ngdm chiet dtfdc trinh bay trong Ba'^ng I.
dung mdi stf dung la ethanol.
^ 3.3. Klid ndng khdng oxy hoa ciia dich chiet tii vo qua thanh long
Ket qud khdo sat kha nang khang oxy hda cua dich chiet ttf \d qua thanh long rudt dd difdc the hien d Bang 2.
Bang 2. Ket qua phan tram gidm dp hap thu theo nong dp trolox
STT
1 2 3 4 5 6 7
N5ng do trolox (HM)
100 200 300 400 500 600 700
PhantramdOgiSmdOhapthu
(%)
11.98 23,12 34,26 45,40 56,54 67,68 78,82
Phan trdm dp giam ha'p ihu cua dich chie't la 46%, nong do theo trolox la 405pM trolox/lit dich chie't (pmol TEAC/L).
Ke't qud cho tha'y dich chie't vd qua thanh long rudt dd cd do giam hd'p thu 46% khi phan tfng bdi gd'c ttf do DPPH. nghia la dich chie't ttf vd qud ihanh long rudt dd co kha nang khang oxy hda.
Bang 1. Ket qua khao sdt cdc yeu to'anh hu'dng qud trinh ngdm chie't
;
Oieti ki&n ngam chiet djch chiel Wvo qua thanh longnjotdo
____
Cac yeu to Nhiet dp say nguyen lieu vd thanh long Ty le dung moi ngam chiet (V/v) ethanol/ nudc Ty le dung mdi nguyen lieu (mL/g) Thfii gian ngam chiet Nhiet dp ngam chiet Solan ngam chiet
Khao sat pH anh hudng den djch chiet
i Dieu ki$n thich hdp sau khi khao sat 60-C
' 60:40
• 25/10 1 4 gid
Nhiet dp phdng Ngam chiet 1 lan pH = 4-6 dich chiet d'n dmh
TAP CIJ CONE TIlTdNG
Hinh theo
1: K4i qua phdn trdm giam dp hd'p thu nong dp trolox
0.ni4)(+0.8435 R' = 0.993
200 400 600 N^ng d9 trolox (pM)
4. Kct luan
Ngbicn ctfu da thanh cdng trong \ic>.' chiei uich vd khao sal kha nang khang oxy hda cua djch chiet ttf \ 6 qud tiianh long rudt dd bdng phtfdng phap DPPH.
Ngodi MCC phdt trien cdc san pham che bid'n ttf thanh long thi van de nghien ctfu tdch chiel chd't chd'ng oxy hda cd trong vd qua thanh long nhdm giai quye't Itfdng phe thdi da md ra nhffng tfng dung trong cdng nghiep thtfc phd'm, dtfdc pha'm hien nay, dong thdi gdp phan tao dau ra on dinh cho llii trtfdng thanh long rudt dd •
TAILlfUTHAMKHVO:
1. Tran Thi Thdy Du'dng. Tu'dng Le My Tii. Diing Ki&u Nhung vd cpng sif (2014), Khao sal thdnh phan hda hoc vd hoal linh sinh hpc cua ttnh dau ngai say (zingiber montanum), Tcip chi Khoa hgc Trifdng Dgi hoc Can ThO, 35 (2014). 131-138.
2. N!;ii>'en Thi Dau (2010). .\ghien ei'Oi ehiel .xud't clid'i mau Betacyanin tif bo qua thanh long Hylocereus spp, Luan
\jn lot nghiep.
.l.Janiilah \;i cpng stf (2011). Physico-chemical characierisucs of red piiaya (Hylocereus polyrhizus) Peel, Iniemaiionu! FOCHI Research Joumal IS. 279-286.
4 N^ii>C-n Cdng Thiiy Tram. Nmh The Sdn. Ngu\en Manh CifcJiig va cong siT (2017), Danh gid hoat linh chong ii\> hoa in vitro ciia cAc phan doan chiel xuat va hdp chat phan lap td qua cay dtfa dai (pandanus odoratissimus!.
F.). Tup chi Y Ducic hoc Qudn sU. 6.
5. Bd Y le t20l7). Difcfc dien \ ici .\ani V. \ \h Yhoc.
Nfid) nh3n bai: 19/3/2020
Ngd> phan bi?n danh gia va su'a chffa: 29/3/2020 Ngin cha'p nh§n dang bai: 9/4/2020 Thdnglin rdc gid:
l.ThS. HI VNH n u HONG HOA :. r h s . N c i v K w i "\N THI DIEM TRINH Giang vien trtfdng Dai hpc Tra ^'inh 3.Lt:THIKlMN(;VN
4.TRl'0Nt; 1 HI NGOC THUY Sinh vien trtft^ng Dai hpc Tra \ inh
290 So 7 - Thang 4/2020
HOA HDC-CONG NGHE THlfC PHAM
AN INVESTIGATION OF THE ANTIOXIDANT ACTIVITY OF EXTRACT
OF HYLOCEREUS POLYRHIZUS. HUSK
• Master. HUYNH THI HONG HOA Lecturer, Tra Vinh University
• Master. NGUYEN XUAN THI DIEM TRINH Lecturer, Tra Vinh University
• LE THI KIM NGAN Student, Tra Vinh University
• TRUONG THI NGOC THUY Student, Tra Vinh University
ABSTRACT:
This research is to extract and analyze the antioxidant activity of hylocereus polyrhizus by DPPH method. The research vi^as conducted with following solvent extraction and extraction condition: the suitable material drying temperature at dCC, the drying time of 30 minutes, the solvent / material ratio of 25/10 (v/w), the percentage of ethanol / water .solvent of 60/40 (v/v), the extraction time of 4 hours at the room temperature and the pH of 4 - 6.. The research's results show that the extract exhibits antioxidant capacit, the absorption reduction is at 46% widi a trolox concentration of 405pM trolox / liter (TEAC/L molar).
Keywords: Extract, husk, red flesh dragon fruit, antioxidant.
