Khao sat sy phan huy toluene va chlorotoluene
boi vi khuan Comamonas testosterone KT5 co djnh trong alginate
Ha Danh DiJc' Tnrong Dai ligc Dong Tliap
Ngay nh^n bai 31/12/2019, ngay chuyen phim bi^n 6/i.2O20; nga\ nhan phan hien 10 2'2020; ngay chap nh^n dang 17/2/2020
Toluene cung nhu' chlorotoluene la nhirng hp'p chat du'o'c sir dung pho bien \'^ thu'6iig du'9'c phat hi^n trong nioi triroTig bi 0 nhiem, nhat la moi tru-oTig nu-trc. \ i khuan Comamonas testosterone KT5 c6 kha nang phan huy hi^u qua cac chat nay. Khao sat kha nang lam sach toluene ciing nhu- chlorotoluene trong moi tru-<mg dat vik nv^c ciia vi khuan Comamonas testosterone KT5 dang ty do \a co djnh cho thay. KT5 du'o'c co djnh trong alginate phan huy c^c chat n^y vo^i loc d^ cao hon so voi dang ty do. \'i khuan co dinh con chju dyng tot Hon so voi dang ty do it dp pH qud thap hay qua cao. Ngoai ra, vi khuan co dinh trong alginate con co the phan huy tot hon dang ty do khi moi tnrcmg bj nhiem kim loai nang. Miic dp anh hu-oTig cua kim ioai nang den sy phan hiiy toluene la As^" ~ Cd^* ~ Hg^*
=:Cu^*> Pb^' = Ni-\
Tir fi/wa: alginate, chlorotoluene, Comamonas testosterone, kim loai nang. toluene.
Ciu sd plian loai: 1.5
Bit vdn d6
Toluene cung nhir chlorotoluenes diroc san xuat mpt lucmg Icni tren toan the giai vi chung dugc su dung plio bien de san xuat nhieu loai hoa chat nhu chat chong chay.
thuocnhupm, thuoc trir sau..., diing trong y te. san xual\ai.
polymer va nhieu san pham khac [1]. Ngoai ra, chiing con dugc sir dung irong chat tay rua va dung moi. Hang nam.
mot lugng lon cac hpp chat nay dugc thai ra moi truong.
gay 6 nhiem nguon nuac. Toluene va chlorotoluene dugc phat hien irong moi truong, bao gom dat, nuac mat va nuac ngam [2-7], ngoai ra chimg con dirge phat hien trong khong khi [8] va thuc ph5m [9].
Toluene va chlorotoluenes ia nhiing chat kha dpc nen viec tay sach chung la can thiet. Mpt trong nhiirng each tay sach chung la diing cac phuang phap sinh hpc. Phucmg phap nay an toan. re tien, than thien vol moi tmong va hieu qua cao so vai cac phircmg phap khac. Tren the gidi da co nhung nghien cum \h cac vi sinh vat c6 the phan hiiy dugc hgp chat toluene [1.10-20] nhimg chi co mot cong bo dugc thuc hien a Viet Nam [21].
M^c dii vay. da so cac khao sat truoc day deu thirc hien trong moi trucmg long va sir dung vi sinh vat dang la lirng ma it de cap den phan hiiy trong dat va sir dung dang co dinh. Mpt trong nhu:ng giai phap co dinh vi khuan la sir dung alginate. Alginate co nguon goc tu nhien. de phan hiiy
'Email- hadanhduc a ^nuiil com
trong moi truong, re tien, de su dung, khong dgc nen Ihircmg dugc diing trong y te, thuc pham cung nhu diing de co dinh vi sinh \at. C. lesloslerone KT5 dugc phan lap tir dal va biin. \a da dugc khao sal ve kha nang phan hiiy nhieu loai chlorotoluene [21 ]. Bai bao nghien cim sir phan hiiy ciia cac hpp chat nay boi vi khuan C, lesloslerone KT5 dugc co dinh trong alginate.
Noi dung va phiTdng phdn nghl6n ciiru Mdi tneimg nuoi cay vi icliuan
Vi khuan C. lesloslerone K.T5 dupc nuoi cay trong dung dich khoang chat co cac thanh phan (g/l) nhu sau: Na,HPO^
2,79; tCH,P^O, 1,00; (NH^^SO, 1,00; MgSO,-H,0 0^20 va 1.00 ml cac nguyen to vi lugng. Cac nguyen to vi lugng bao g6ra H,BO,0,30: CoCl,'6H,0 0,20; 2nSO/7H,0 0,10;
Na,MoO^ 2H,6 0.03; MnCl, 4H,0 0.03; NiCl,'6H,0 0,02: CuCl, 2'H,0 0,01. Do pH ciia moi truong dugc di^u chinh bang dung djch HCI va NaOH. Moi truong duac khii triing o nhiel do 121 "C trong 15 phiit truoc khi sir dung. Tat ca hoa chat deu dugc mua tir Sigma-Aldrich hoac Merck voi dp tinh kh\k >98%.
P/iiroTjgplidp CO dinli vi Ichiian trong alginate Vi khuan duoc nuoi cay trong moi tmong khoang nhu tren a 150 \/ph va o nhiel dp phong (tmng binh la 30"C).
Toluene (2 mM) \a yeast extract (1,0 g/l) dugc bo sung
I Khoa hoc Tu nhien
Determination of degradation of toluene and chlorotoluenes b> Comamonas testosterone KT5
immobilized in alginate
Danh Due Ha' t)iing Thiip Lnnfrsit)
Received .11 Dccemhcr201Q. at-cepicd 1" hchmarN 2020 Abstract;
Toluene and chlorulolucncs arc ^^idel^ used ingredients in industries and c o m m o n h delected in polluted environments, especially in wa.sfcwalcr. Comamonas testosterone KT5 thai effecti\el\ degraded these c o m p o u n d s ^va.s determined to disintegrate the toxic chemicals in liquid culture a n d soil. The results presented thai the bacteria immobilized in alginate degraded toluene a n d chlorotoluenes more elfrclivcly than those of free cells. Besides, the immobili/ed cells showed higher toleration In extremcU low and high pH levels.
Moreover, the immobili/.ed form reduced the efTect.s of heavy metals d u r i n g the degradation process. The effects of heavy metals on toluene degradation \Nere in o r d e r of A s ' = ( d ~ !![;• =< u = > P b = - = Ni- .
Hfvwords: alginate, chlorotoluenes. Comamonas testosterone, heavy metals, toluene.
dassificafion number: 1.5
n,6i inian- luc nay imng binh la .'.^> w i- ' •- ^^ ^.^
hdn- so khuan lac \uat hi^n tren lh,icli .ig.ul • au - m o r t m o n . long dugc K tarn a 8 000 v ph trong 0 phut- M khudn thu ducTc dugc chuyen ^ao mo, irumig k h o a n j khuiN d^u r6i dugc 1> tam l^n 2 nham lam sach hoa U u th^ con sot la, bam ^ ao ^, kl,u.in. \ , khuan sa,. do J L ^ C ho
%ao mo, irucme khoang moi. trgn deu \ a , dung ui^' a!e,naic da khu trung d^ thu dugc dung dich cuo, cung r > . 1 i r CFU/ml \ a 3 % a k m a i c . Dua vao so lua,ig v i k luan ban ddu ^d so lugng hat alginate [ao ra >6 lugng M khuan imng binh trong mm hat dirge \ac duih.
Qua Irinh CO dinh ^ i khudn dugc thuc hien bSng each:
d u n . dich chua ^, khuan va alginate duoc nho gipt vao dung dich chua ^^% CaCl, bring syringe. Dung dich 3 % C a C U a y duac khud\' dC-u bang khu:V lir o toe dp 500 y/ph trong suol qud trinh nho gigi Hal dugc lao ra, rua lai roi chuyen sang djch chua .v.. CaCl, vii bao quan trong 24 gia o nhiet dp 4"C dc hoan tai qua trinh co djnh.
Thi nghii'm phan hiiy toluene va chlorotoluene trong moi trurmg Idng
Olid trinh phan hiiy hoa chdl ciia vi khuSn dupc thuc hien o 150 \ ph \ Li nhiC'I dg phong voi n6ng dp hoa chal la 2 m M trong 12 g,o. D 6 , voi thi nghiem anh huong ciia kim loai den su phan huy. kim loa, nang bao gom As^' (As,05), Cd^"^
(CdC!,), Cu-' ( C u C V 2 H , 0 ) . Hg=' (HgCl.) va Ni"^^ ( N , C y dugc ihem \ a o moi Irucrng khoang vai nong do cac ion la 2 mM Lugng vi khuSn trong dung djch la 3,5>: 1 0 ' C F U / m l ap dung cho ca thi nghiem phan hiiy bai vi khuan lu do va
\ i khuan co djnh.
Xde dinh su' sinh tnrdng ciia vi Ichudn trong moi tru&ng long va trong alginate
So lugng V, khuan Irong moi truong long sau mpl Ihai gian u dugc xac dinh dua vao so lucmg khuan lac hinh thanh tren dTa agar. Phuang phap xac dinh so lugng vi khuan trong hat alginate dugc thuc hien theo mo ta ciia Schoebitz va ctv (2012) [22]. Mo, truong sau khi ii d u a c b6 sung N a N O , den nong dp 6% va ISc deu cho den khi hat alginate tan het (khoang 10 phut). Dung djch d u g c pha loang r6i Ira, dSu tren dTa agar chua moi trucmg khoang chSt da mo ta a tren va dem so khuan lac hinh thanh sau 24 gio ii a nhiet dp 30'^C.
Thi nghiem phdn hiiy toluene vd chlorotoluene trong
Dat duoc thu tir vuon cay trong T m o n g Dai hgc D6ng Thap o dp sau 10-50 cm, nai chua timg bi n h i i m toluene hay chlorotoluene Mau dat sau do dugc bop nho bSng tay, irrm deu ro, ray sang qua lam luoi thep cho phep nhCrna hai CO kich thuoc nhc> han 2 mm di qua. Mau dal d u g c xac dinh
!h_;ri- rhdn trucVc khi t,en hanh thi ngh,em (bang 1).
KHOAHOC \ \
Bdng 1. Th^nh phan cua d^t (tinh theo tri?ng luvng dat kho).
Thinh p h i n Cdt Phusa DSi sol Chit hihi CO Nim Phdlpho pH Kho, lirgng riSng Toluene v^ chlorotoluene
Ham lirvng 43.6=5,6%
34.7±7.7%
19,0±6.6%
2 l , 4 ^ ? . 5 g ; k g 1.2"=Ii4gl.a 1.13=0.2 gfl(g 6,6±0.7
l.56±0.3g/cm' 0
Doi vol thi nghiem phan huy hoa chat bang vi khuan tu do, vi khuan dugc lani giau trong moi trucmg khoang roi Ml vao dat va trpn deu. Tutmg ty, doi voi vi khuan co dinh trong alginate, hat alginate cung dupc trpn lan voi dat.
Lugng vi khuan dugc bo sung vao dat a nghiem thirc vi khuan co djnh va vi khuan lu do deu la 10'' CFU/g dat kho Dat dugc bao quan trong binh nhua, day kin bang nap. Dat dugc them nuac de co do am 25%. Hang ngay nap dugc ma ra chimg 2 phiit de O, co the khuech tan vao va CO, khuyet tan ra ngoai. Dat cung dirge lac deu 1 lan klioang 5 phiit de Irpn lai va de oxy khuyech tan xuong lop dat sau. Do day ndp thuong xuyen nen do am trong dat thay doi khong dang ke trong suot qua trinh u. Cac nghiem thirc doi chiing, alginate khong chua vi khuan duoc trgn vao dat.
Miiu dat dugc lay de phan tich hoa chat con lai sau mpt thai gian i,. Toluene va cholorotoluene trong dat dupc trich ly bing methylene chloride (CH,C!,)- 5 g dal dupc cho vao dung djch chua 15 ml methylene chloride, them nuoc de dugc 25 ml roi trgn deu, vortex trong 5 phiit. Mau dugc de yen trong vong 10 phiil. Phan methylene chloride phia tren tach khoi dung dich dupc chuyen sang ong tube mai.
Qua trinh nay dugc thirc hien 2 lan. Dung dich methylene chloride duoc lam sach bang 0.22 jim syringe filter. Hieu qua thu hoi trung binh cua toluene, 2-chlorotoluene (2CT), 3-chlorotoluene (3CT) va 4-chlorotoluene (4CT) tir dal tuong ung la 92.4. 93,0. 95,5 va 96,6%.
Scanning cdu true hen trong cua alginate Qua irinh chup hinh cau triic ben trong ciia hat alginate dupc Ihuc hien tai Da, hpc Chulalongkom, Thai Lan. Qua trinh scanning dugc thuc hien nho may Scanning Electron Microscope Analysis (SEM). Hat alginate dugc co dinh bang 1% osmium tetroxide (Op^) 1% trong 24 gia roi dupc nja lai bang nuoc nguyen chat. Mau dugc dehydrate hoa bang ethanol \hn lugt la 30. 50. 70. 90% va c6n tuyet d6,.
Mau sau do dugc lam kho bang CO,, dugc dinh khung bang vang-palladium, roi dugc scanning bang JEOL microscope (.ISM-541OLV, Jeol, Tokyo, Nhat Ban) a 15 kV.
Phircnig phdp plian tfch
Nong dp cua hoa chat dugc phan lich bang HPLC \a GC MS da dugc mo la theo nghien cihi [21]. Cac thi nghiem dugc lap lai it nhat 3 lan, so lieu dugc .\u 1\' bang phan mem Excel 2010 \a SPSS 22.0 voi miic do tin cay la 95% tir phan Iich Duncan tesl.
K^t qua va thao ludn
Khdo sdt khd ndng phdn hiiy toluene vd chlorotoluene ciia C. testosterone KT5
Cac hai alginate sau khi hinh thanh co hinh ciiu ho^c hoi bau due, vdi kich thude khoang 3 cm va cac hat co kich thude gan nhu nhau (hinh 1). Cac hat dupc bao quan d nhiet do 4''C neu chua sir dung ngay. Kiem tra su phal lan ciia \ i khuan ra dung dich trong qua trinh co dinh cho thay lugng VI khuan thoat ra ngoai moi trudng long la 3,6x 10'' CFU/ml, tire chi chiem 0,l%i. Hinh scanning ben trong hat alginate cho thay, chiing co cac khoang nho de moi trucmg co the khuech tan vao ben trong, vi khuan co the nhan dugc dugc 6\\ de hoat dgng. Tren hinh ch^ip, \i khuan dinh vao cac thanh alginate d cac klioang rong ben trong. Vi khuan sau khi dugc co dinh trong alginate, chung it di chuyen nhung thyc hien dugc cac hoat dpng khac, vi, chung il tiep xiic vdi cac chat trong moi trudng han.
Hinh 1. Hinh anh chup hat alg,nate (trai) va mat scan cat ngang cua hat alginate (phai). Hinh scan cho thay vi khuan du^p-c co dinh trong hat. Hinh anh hat alginate ben ngoai va scan cat ngang if dp phong dai tu'cng irng la 1 va 5.000 lan.
0 pH=7, vi khuan dupc co dinh trong alg,nate co toe dp phan hiiy cao hon so vdi vi khuan lu do cho dii chiing c6 so lugng nhu nhau. Tir hinh 2 cho thay, toe dp phan hiiy 2CT
\a 4CT bdi \\ khuan lo liing la nhu nhau, va cham hon so vdi toe dp phan hiiy 3CT va toluene. Sy phan hiiy toluene dien ra nhanh hon so vdi cac monochlorotolucnc, ket qua nay cQng duoc mo ti, tir cong bo trudc day dugc thuc hien bdi Due (2017) [21]. Sy phan huy toluene va 2CT. 3CT va 4CT dugc thyc hien do vi khuan co dinh co loc do khac biet khong dang ke. Sd lugng vi khuiin sau 12 gid ii tang len lir 1.22 den 1.84 liin so vdi ban diiu. Sau khi ii. so lugng vi khuan trong moi trudng c6 chat de phan buy hon co phan
I Khoa hoc Tu nhiin
nhicu hon so \ o i moi iruimg co chat k-. phdn hu>. nhung trong moi trucmg chung \ i khuan lu do \ 3 M khudn cd djnh khdng khac nhau dang kc (hinh 2B). Sau 12 gio u. mgi so
\ i khudn Irong hjl alginate phat lan ra moi iniiTig long So lugng nay dao dpng troni: khoang tu 16 den 2 " long si' \ , khudn.
Trudc da> J.T co cac dong \ i khuan phdn hiiv chlorotoluene nhu Hhodococ. in sp. OCT 10 II ]. Ruhionui sp [*S 12 12^1 BurkJiolJeria.PS]2\l-i\\2.Acljn>niohh-lcr sp, K\^ I |251 duoc cong bo C.K dong \ i kiiujn khac co kha n.ing ph.in liu\ lolucnc nlumg ktiong dc ^Jp ilcn phan hu>
chlorotoUicie nhu niol so dong dirot pti.ui k"ip NTI Jacob \ a Irshaid (2015) tir dat d nhiem [26]. mgt so dong \ , khudn phdn hiiy toluene phdn Igp tu phdn bo | 2 " j \.i moi d-'iy nhdl la Acincliihiiclcr /unn CH005 phdn lap Iir JJl o nhiem dau [2S]. Nhung sy phan hu> cua cdc ddng \ i khuan na> dugc thyc hivn a dang ly do \ a khong khao sal anh hucimg cua pH cung nhu kim loy, n,'ing den loc do phan hii\ \ lec khao sal kha nSng phan hii\ d tr^ng thai co dmh \ a anh hudng ciia moi Irudng ile,i sy phan hu\ giiip ta cd the ap d\ing trong Ihyc le
4nh tome . " . - * pH * " -VP'"'" '"'>; " ' " ; "'• " ' " "
| . h u>.,,. -.. phan Ino' toluene .v c c J o pi I kh.K n t a u cho
^ i , , . , , ; , l,oa chi. con 1... o cac J o pH 4>.a -hap h.n q "
. ; • . . , nh.Ou hem a J o pH - - (hinh 3). dicu na; . :..n.c 10 r C . - \ ' , khuan kha nh,y cani v CT, sir IhaN J o , cua pi I O ..K J c pH khac nhau, >oc dP cua ^ i khu,m co d,nh Jcu n ,,, h™ ,., >m d,,nE lu do va su .hay J o , pH ,1 lan, .ha> .>.-i toe JohLTn .0 vol dang nr do.
Tai ca c ic irutrnc hgp phan huy trong nui, trUOTg long da mo ta tren dav cho thiy. vi khuan d w c co Jinh^co toe J o cao h,m han dang tu do. Cass,dy va elv cho rang, sir
^,,^, ;.p ^,n. irirc uep >o, hoa chat khi dugc co d,nh trong alsmale g,up giam linh Joe ha, d i n v, kliuan. giup lam tang I6c do phan huy [29|.
100 I \ 1 kliii.ii
• \ ! k]„>.ll
III
Toluene 2CT
• \ 1 UU1.-U1 ut do
llll
Tolueue XT .KT -Hrr Hinh 2. Sy phSn hiiy toluene va chlorotoluene boi C testosterone KT5 (A) vd s4 lif^ng VI khu^n iy d?ng t^r do va co d[nh trong alginate (B). Thi nghifm diriTC tien hanh trong moi trmnig long cd d$ pH=7 voH ning d? hoa chat la 2 mM va thoi gian ii la 12 gic/.
• Vikluiau l\ido
pH = 5 |)H = 6 pH = 7 pH = 8 pH = 9 Hinh 3. Anh huwng cua do pH den sy phan huy toluene cua C.
(estosrerone KT5 is dang ty do va co dmh trong alginate. Thi nghifm dip^c tien hanh b nong do hoa chat la 2 mM va thm gian u la 12 gicr
.Anh hirifng cua kim loai nang den suphan hiiy toluene bdi vi khudn C. testosterone KT5
Cdc kim loai nang co the ton tai Irong dal hay trong n u d c
\ a anh hudng den cac hoat dgng ciia vi sinh vat. K.im loai nang co mat trong dal da dugc khao sat d mpt so dia p h u a n g nuoc ta [30]. Danh gia mirc do anh hudng cua kim loai nang den su phan huy toluene trong moi trudng long cho thay, vi khuan duac co dinh co toe dp phan hiiy nhanh hon so vai vi khuan tu do. Su co dinh trong alginate, Idp alginate bao ve cho \ , khuan giup chung giam d u o c su dgc bai cua kim loai nang so vdi tiep xiic trye ti^p trong mdi trudng long.
Trong so cac kim loai nang dugc khao sat. As'", C d ^ \ Hg-" \ a Cu-' CO mirc dp irc che tuong d u o n g nhau. Su phan hiiy toluene, Pb-" va Ni-" cung cd miic dp dpc doi vdi vi khuan tuang duong vii h hem so vdi cac kim loai neu tren (hmh 41 Nghien ciru Irudc day dugc tien hanh boi Lin va cs I2MI|4) cho thay, Mycobacterium sp. C H X Y l 19 duoc c6 dinh irong polyvinyl alcohol - alginate ciing co kha nang chiu dyng duac vdi nong toluene va mpt so chai hCru t o khac cao hon so vdi dang lu do [31]. Mycohm h '-mm sp
KHOAHOC \ . \ ttNGHGWm Vill
Doi chiing As{V) Cd(il) Hg(ll) Pb(l() NiOl) Ci<II) Hinh 4. Anh h u d n g ciia kim loai nang den s u phan huy toluene cua C. testosterone KT5 6* dang t y do va co dinh trong alginate.
Thi nghiem dirax; tien hanh if nong do hoa chat cung n h u kim loai n^ng la 2 mM va thdi gian u la 12 gid.
CHXYl 19 dugc cd dinh cung il bi anh hucimg bdi kim Ioai nang nhu Mn' . Ni-', Zn-' hem so vdi dang ty do. Ket qua nay cho thay sii dung dang co djnh co uu the hon khi xir ly 6 nhiem toluene trong cac dieu kien moi trudng khac nhau.
Phdn hiiy toluene vd chlorotoluene trong ddt bdi vi khudn C. testosterone KT5
Trong cong bo trudc day, C testosterone KT5 dang ty do da dugc khao sal ve kha nang phan hiiy toluene va 3CT va 34DCT trong dat, Trong bai bao nay, su phan huy cac chat nay ciing dugc thyc hien nhung cd so luong vi khuan cao hon. Ket qua cho thay, vdi lugng vi khuan cao hon thi toe dp phan hiiy trong dat cao hon. Chang han, trong bai bao trudc, so lugng vi kliuSn la 10'' CFU/g ddt kho. 78,6±4,7%
toluene bj phiin hiiy sau 15 ngay [21]. Trong thi nghiem nay.
Bang 2. S y phan huy toluene va chlorotoluene trong dat sau 7 ngay ciia v alginate.
phan tao ra sy khac biet ve toe dp phan hiiy.
Tir bang 2 cd the thay rang, sy chenh lech \e kha nang phan huy ciia \ i khuan co dmh \ ii ty do trong dal la khdng nhieu nhu trong mdi trudng long. Mac dii cd the chju dyng dugc dpc to ciia moi trudng tdt hon so vdi dang ty do, nhung vi khuan bj cd dinh go bd trong khoang khong gian hep hon nen khong duoc tiep xiic vdi moi trudng ngoai trong thdi gian dau. Sy kliuech tan hda chat trong dat bj han che cung la nguyen nhan lam vi khuan b\ co djnh kho tiep xiic vdi hda chat. Sau khoang 5-6 ngay. cac hai alginate co hien tugng vd ra hoac phan hiiy mot phan. Alginate cd ngudn goc tir cac loai tao niiu (Phaeophyceae) nen viec bi phiin hiiy la bmh thudng va gop phan phdng thich vi khuan ra mdi trudng ngoai. Toluene de phan hiiy trong dat hon so vdi cac chlorotoluene khac. Mpl nghien ciru trudc day cho thay, co dinh vi khuan Pseudomonas sp UG14Lr trong alginate de phan huy hoa chat irong dal lam giam kha nang song sot va phan hiiy so \di dang ty do [32]. Do am trong dat thiip lit yeu td chinh dan den sy giam sut nay [32].
6 mpt so nghiem thirc, sy phan hiiy ciia hoa chat trong dat chua khir triing nhanh hon diit da khu triing la do boat dong cLia vi khuan trong dat. Vi khuan da chimg viio moi tnrdng dat chua khir triing phan hiiy nhanh ban dat da khu triing d mpt so trudng hgp, chirng to vi khuan dugc chung CO su cong tac vdi vi khuan ban dja mil khong bj vi khuan bim dja canh tranh bay irc che, Trong cac nghiem thirc ddi chimg khong chiing vi khuiin, su giam lugng hda chat la khdng nhieu, chiing to vi khuiin C lesloslerone K.T5 phat huy dugc \ ai tro ciia chiing.
1 C. testosterone KT5 dang t y do va co djnh trong
Toluene vJ chlorotoluene con lai trong dat ( % ) ' Nghif m tbi>c Alginate khang co vi kliuan fl khuan lir da
Ddi khang khu iriiiig Dal da khu irimg Ddi klidng khir Irung
Vi khuiin CO djnh Ddt da khir iritng Dal khong khir Irimg Toluene
2CT 3CT 4CT
88,3+6,3"*
90.5±4,2''>
91,0*4,0"*
94,1 ±3,4"*
92,3*3,6'-"
96,5±2.7-*
95,0±2,2^'*
94.5±3,8"*
35.5*7,3'''^
68.5±8,7""
42.4±|],1''*
51,1*10,8^'"*''
41.2±8,8'"- 75.5±8,6'"»
65,4±10,2'8 6U±8,4''«
26.5±6,9'^
53.9^10,0'"
35,7*11,2'^
42,5±7,9"^"
40,2±7,7"
M . S i l l , ? ' ' ^ 44,7*8,7-*"
61,5±8,7'"^
•• cSc ch& in thuo'ng khac nhau di kem vm cac so the hien su khac nhau co y nghTa thfing ke trong cung mot hang va in hoa th^ hign trong cung mpt cot Sy khSc biet n^y co y nghia th6ng ke 6' mine p<0,05
\di 3,5x IC CFU/g dat thi toluene da hoan toiin bi phan hiiy trong dat sau 10 ngay, Tuy nhien, sy phan hiiy trong dat dien ra cham han rat nhieu irong moi trudng Idng. Dieu nay cung dugc xac nhan d bai bao trudc. Trong nidi irudng dat. sy di chuyen ty do ciia \i khuan. cung nhu su khuech tan cham ciia hda chat va oxy thudng lii nguyen nhan chinh dan den su phan huy cham hon d moi trudng Idng. Ngoai ra. cac yeu to khiic nhu thanh phan va ket cau ci,a dat. dp pH ciing gop
Ket luan
C. lesloslerone KT5 dupc phan lap tu dat bj d nhiem co kha nang iing dung de lay sach toluene va cac monochlorotoluene khac ton du trong dal va trong nudc. Vi khuan co dinh trong alginate the hien kbit nang phan hiiy vugl trpi so \di dang ty do trong dieu kien thudng hay khi dp pH thay doi \a sy nhiem dpc ciia kim loai nang.
Khoa hQC Tl/nhien
TJU U t I T U M KHAO
J | n I J o h i b w . k . H t n g c ^ s c ^ i 2 l l l ; i "I>i:f3diHon i^! Z-^M Mi>t'luc'-- ft) ,Jl'feJ.K,l,,ln^p LKT 111", (p," Mun-h.,-,: B^:-u-.hn. " i i f i - • ; : <-
\1\ L.ll ki.i,h (["JM ( i - . . . [wlluianii in water iJiti ili,:a ?itn ind anaK-iis- D i i w m ."v., Ti •• 15i;i pp 1 5 6 - ! ^ :
[l) KM ^.nl! W l ) . No.i',.jni t l - ' V ' . Xn^mk-al momimny ol Wi'-i.iii.iii'sgRmniJ water"..' I l t i l t "S ^ p ' i i . " *
|4] I Mani, R [ l o r d , J M a m n - \ k ' n ^ > i \ c n m r a i:iMl5i
"IX-iiTminjlinn ot"chlormj,i-ii Uiiuoni.-- in ravv and ir,-.!:L-J MJILT ^jmpic^
from thi.' I kihrcyal nver b\ <Uf^-d k v p .inppini: anaK-i> jnd y.i- chri'mjlro^rapliN•ma.'isspcclromoin dciecimn".^ ( i"^-m^u-yr i. I i r ' i l i . pp M-^i
I S M ) ^ikolanu. U (lollinopvulos. M N ki'.li..pi>ulou. G \ Ki>li>kMli.is, 11) Ickkjv i:it,i;i, "IX'terminaiion of uilaiilc otvamc compounds in surkiL-t nati'rs and treated » - .M,i:cr in iirii-ie", liuur Rt\.
.16(111 I T : S S > : ' ^ > ' N
| ( ' 1 J J WcMn<:k.J\\ Moll>. R l ^homaMW.^4l -The gR-undttata
•iiippK %uni->",j -i/H JI.;/,. l^,-/i^.^,^u,h -t. p p > : . ^ ' ( n I H 1 Ai'tenian K Harden. ) H I! i Lindi-r^. C I I I \U<m W Slooil I I'lKlll, ""i'lTM-k'n, urganii- pi'lkilanis in rncr water and ground wJii'f ol ilif \oilaTl.iiidv (ittnh.^pli.n fiA] pp :.«! Za'*
|N] S S Huli.imra i l'>
.ideiia-'.inKiiMati .Am/,
analv-i- ol M i l ' s vuni>\ data from . « i i i , r r : 4 5 - : ^ -
| 9 ] 1 ) 1 Hcikfv S R Icnveii.Ml nemmi:-Joni:s (I'WM. 'Puivo and trap fvirac,1011 wi,h lit. -M'^ dcii-niunaiionof volaiilir orizann. compounds in lable-rcud) k>i>ds".J J^n. Foixi Chem 4 . l | l l ) pp X i y O s " " ;
| m ] l l Bnnkmann. W ReinckiMW":!. •Degradation ofLhlorololucncs by m MMI lonsimck'd li\hnd •-irains prohleni' ot en/\mi' spciiiMi).
mducion and prL-\i'ntuni ol nj,/,i-pj[hna>". I i.MS \li,n.hiol It-ii. "Sill p p S l - r
| l l | H r IktiykT.t. \ PcititrL'w, J C Spainil'*'):). "BK\legradaiionol*
miMua's Hi" suhs|i,u,ed Ivn/L-nfv hv l\auit>m(mji sp *tniin JS15(r. ^ppl Fiiiinm W(,m/./,i/.5S(-i p p : : ! ' - : : - i 4
| i : | M \ H.iro. \ Loren/o l2iKin, "Mi'iaKilic engmeenng ofbaciena lor L-nMronnicnt.il applnaiions i-oii^iniaion ol" P^v/i./iw/iii/.u strains for bi.Hlcgr.idalion ol :-ehlorololuenf". J liwu.': 85(2) pp !|).?-1I3
| n | . H i U - ^ l i v . k l l T r a a . M H l l o C t W . - l . D e m d i l i o n o r v o l a i i l e hydrocarKins iVoni steam-cljssilit'd -olid l^a^le b> a niiMua' of aroma tic h\ dro^MrKm-dcLiradm^ baciena", Bwii. im,il I cn . 25(6). pp 4 ' i - l S . i
i l 4 H ) , V M a l t s e \ a . i r s o h , i i i i k i n j , L A C.olo\lL-va.M Schiomann.H.J knj.-kmiiss i l W 4 l , "Dienlai-ion l!>drolase from /f'w./w<>.</n cnihnipolu H.P punrk-iiioiiandprnpenieV. inh Mumbiol. IbliS) pv- --h^-^-J
ll.*;! r \ i s h i o . -V Pjk-1. V Wang. PC.K Lau i2fi()l|,
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l^l k Pollmann. S Beil, D H Pieper i : a i t | l "Tranforaiation of chlonnali-j K•n/c^e^ and toluenes h> TecA teiraJiloroK-n.^L-nc d i o w c e n a . e and T « H .-hlorokn/cnc dih\dr\\liol deh\drogenase of Siv/wiiy/iu s p ' s t r a m P ^ i : - , Vv-.' hy..:>, l / ; . r „ . „ (,-(«, pp Jn5--4()C3
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der Mcer H P ! k o h : . - . ^ i - * ; i t3;oir.in-fonti .•lo" •• • • • , , --.'. m- •
t'/irvn'"
iRXTLiK. -p,uJ'!d-ihfJiiraldih\an.\iib>drov\.'.-nv • -"^
l / , - i O i ^"(Sl j.p.V.r.-5?50 ^ . : < : P sarder. R \ l WiU's-h. P I o r r u a ' l . H ^^^;^,^'^„ , , , ^.„,. by
, l-w"] K "DciiT^dauon of 1 ;.4-irKhloro- and I : 4.^-ieli. i- •'• • ~ / ' . , : , . . . . , . . . : - , r a m s - . V r / £ ' . u / . i . l / . . - " - ' ' ^^<>' ^ ^ ^ ' • " ' " "
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coT-^Mffld." ippl l-inimii Ui."-hioI.-i2l-tl?? - - ' - ' - . t. II .lOiKi ••Mineralizalii'n o r , : , ] , . ^ , , J . > . R i : » . » « c \ " ' * ' ' , ' „ „ " r f c h l o r o a n d
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•ttlet
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