Nghien CLFU - Ky thuat
Tai lieu tham khao
1. Hutchison I et al. 14. J. Med. Chem., 2001, 44 (9), 1446-55.
2. Matysiak J et al. J. OAAC. Int, 2004, 87 (3), 579-86.
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5. Stephenson KA et al. Bioeonjug Chem., 2007, 18(1), 238-46.
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7. Nam NH, Dung FTP, Ganh DTK, Thao TP, NgpcNA. Tap chi Dugc hoc 2008, 12, 31-35,.
8. Dung FTP, Thao TF, Nam NH. Tap chi Dugc ftoc 2009, 2,29-33,.
9. Dung FTP, Thao TF, Nam NH. Tap ehi Dugc hge 2009, 5, 43-47,.
10. Larry M, Kandel J. Microbiology: Essentials and applications. Inc New York. 1996.
11. Skehan F, Storeng R, Boyd MR. Joumal of National Cancer Institute 1990, 82, 1107,
12. Wu L, Smythe AM, Shoemaker RH. Cancer Research 1992, 52, 3029.
Thanh phan hoa hoc cua tinh dau la re xanh {Cinnamomum tonkinensis (Lecomte)
A. Chev.) & Viet Nam
Nguyen Thi Hien', Le Cong SonS Do Ngoc Dai^
TrSn Dinh TliSng', Tran Huy Tliai^
'Trudng dgi hoc Vinh
^Vien ST&TNSinh vat, Vien Khoa hoc Viet Nam
IVIo'dau
Chi long nao {Cinnamomum) Id mpt chi Idn trong hp long nao (Lauraceae) co g i n 250 lodi phan b i nhiiu d rung mua nhiet ddi thudc cdc viing D6ng Nam A, Trung Quic, An Dg...'^'. &
nude ta, s i lodi thugc ehi long nao {Cinnamomum) r i t phong phu vd da dang vdi 40 lodi '^', r i t nhiiu lodi trong ehi long nao Id cay tinh d i u , cay thuic, cay gia vj, cay l i y g i eo gid trj va lam nguyen lieu d i u eho e6ng nghiep '^l Tinh d i u eua ehi long nao {Cinnamomum) Id d i i tugng nghien ciru cua nhiiu phong thi nghiem tren t h i gidi va thudng dugc dung Idm nguyen lieu ting hgp hCpu ea do ehiing co hdm luang eao eae hgp chit nhu linalool, camphor, einnamaldehyt, eugenol, safrol, metyleugenol, terpinen-4-ol, a-terpineol... '"l
Cay re xanh {Cinnamomum tonkinensis (Lecomte) A. Chev.) thupc hp long nao (Lauraceae) phan b i d Thanh Hoa, Nghe An, Ha Tinh, Thua Thien H u i (Bach Ma) vd Van Nam (Trung Quic).
Cay g i cao khoang 12m, mpc rai rde trong omg nguyen sinh, ven rung, d dp cao 800-1500m. Cay ndy Id nguon eung c i p g i tit, b i n de diing trong xay dung va dong do gia dung '^'^l
Trong chuang trinh nghien cuu mpt each he thing cua chung t6i v i mat hod hge nhim phuc vu cho viec tim kiim cae loai tinh d i u vd cdc boat ehit mdi gop phin eho c6ng tdc diiu tra ea ban nguin tdi nguyen thien nhien phong phu eiia viing B i c Trudng San, djnh hudng cho viec su' dung nguon tdi nguyen ndy, thdnh phin hod hge cua cay re trung Id d i i tugng nghien cuu d i u tien cua ehiing tdi v i ehi nay. Han nii'a sau khi tim kiim tren mang vd tham khao cdc tdi lieu chiing toi ehua thiy mpt eong trinh nao d nude ta vd tren t h i gidi nghien ciPU v i tinh d i u cay re xanh {Cinnamomum tonkinesis (Lecomte) A. Chev.).
Phin thye nghiem Nguon nguyen lieu
La cay re xanh {Cinnamomum tonkinensis (Lecomte) A. Chev.) duac thu hai d V u d n Q u i c gia Vu Quang, Hd TTnh vao thang 1 nam 2010.
28 TAP CHI DUOC HOC - 9/2010 (SO 413 NAM 50)
N g h i e n CLFU - Ky t h u a t
Tieu ban cua loai nay da duac so mau tai Vien sinh thai va Tai nguyen sinh vat, Vien Khoa hoc Cdng nghe Q u i c gia Viet Nam va luu trip d Trudng Dai hpc Vinh.
Tach tinh dau
La tuai (2kg) duac c i t nho vd chung c i t bing phuang phap chung c i t loi c u i n hai nude trong thdi gian 3 gid d dp s u i t thudng theo tieu chuan Duac dien Viet Nam '®'. Ham lugng tinh d i u la tinh theo nguyen lieu tuai Id 0,75%. ,
Phan tich tinh dau
Hod tan 1,5 mg tinh d i u da duge lam khd bang natrisunfat khan trong 1ml hexan diing eho s i c ky.
Sac ky khi (GC): Dugc thuc hien tren mdy Agilent Technologies HP 6890N Plus g i n vao detector FID cua hang Agilent Technologies, My. Cpt s i c ky HP-5MS vdi ehieu ddi 30 mm, dudng kinh trong (ID) = 0,25 mm, Idp phim mong 0,25pm da duge SLP dung. D i i u kien phan tieh nhu cdng b i trong cdc bai bdo trude day cua chiing tdi '^'. Khi mang H2. Nhiet dp buing bam m i u (KT thuat chuang trinh nhiet dp-PTV) 250°C. Nhiet dp detector 260°C.
Chuang trinh nhiet dp buing d i i u nhiet: 60°
C (2 phiit), tang 4°C/phiit cho d i n 220°C, dung d nhiet dp nay trong 10 phiit
Sac ky khi-khoi pho (GC/MS): Viec phan tieh djnh tinh dugc thuc hien tren he thing thiit bj s i c ky khi vd p h i ky lien hgp GC/MS cua hang Agilent Technologies HP 6890N. Agilent Technologies HP 6890N/ HP 5973 MSD duge lip vdi cpt tdeh mao quan vd van hanh s i c ky nhu d tren vdi He lam khi mang.
Viec xae nhan cdc c i u tip dugc thuc hien bing each so sanh cdc dip kien p h i MS cua ehung vdi p h i chuin da duge cdng b i eo trong thu vien Wiliey/Chemstation HP. Trong mot s i trudng hgp duge k i i m tra b i n g cae ehdt trong tinh d i u da biit hoac c h i t chuin '^"^^'.
K § t q u a v a t h a o l u a n
Thanh phin hoa hge eua tinh d i u Id cay re xanh {Cinnamomum tonkinensis (Lecomte) A.
Chev.) d Hd TTnh da duge nghien euu. Bing phuang phdp s i c ky khi (GC) vd s i c ky khi khii p h i (GC/MS), cho thiy cd han 40 hgp ehit, trong do 31 hgp chit da duac xae djnh chiim 95,2%
cua ting ham luang tinh d i u . Thanh phin chinh cua tinh d i u la cinnamyl aldehyd (32,6%), p- phellandren (14,7%), a-pinen (12,5%), linalool (11,3%). Cac c i u tip khac it ban la a-pinen (7,5%),
TAP CHI DUQC HOC - 9/2010 (SO 413 NAM 50)
1,8-cineol (2,5%), p-oeymen (1,6%), myrcen (1,9%), benzyl benzoat (1,6%), P-earyophyllen (1,4%), sabinen (1,1%) vd a-terpineol (1,1%) (xem bang 1), Cac chit eon lai phan Idn eo hdm lugng tu 0,1% d i n 0,9% (xem bang 1).
Bang 1: Thinh phin hoi hoc cua tinh diu li ciy re xanh (Cinnamomum tonkinensis) a Hi TTnh
TT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Hgp chat a-thujen a-pinen camphen sabinen P-pinen myrcen a-phellandren a-terpinen p-ocymen p-phellandren 1,8-cineol (E)-P-ocimen Y-terpinen a-terpinolen linalool borneol terpinen-4-ol a-terpineol cinnamyl aldehyt bornyl acetat eugenol a-copaen P-elemen P-caryophyllen a-humulen bicyclogermacren (E)-nerolidol spathoulenol caryophyllene oxit Guaiol
benzyl benzoat
Kl 930 939 953 976 980 990 1006 1017 1026 1028 1034 1052 1061 1090 1100 1167 1177 1189 , 1219
1289 1359 1377 1391 1419 1454 1500 1563 1578 1583 1601 1760
TyleVo 0,3 12,5
0,6 1,1 7,5 1,9 0,7 0,1 1,6 14,7
2,5 0,1 0,2 0,1 11,3 . 0,1
0,3 1,1 32,6
0,2 0,2 0,1 0,1 1,4 0,2 0,6 0,5 0,6 0,3 0,1 1,6 vet < 0,1
Kl = Kovats index
H i u h i t cac lodi thugc chi long nao {Cinnamomum) d i u cd chira tinh d i u hoac huang tham, song hdm lugng vd thdnh p h i n hoa hge cua tinh d i u d m i l loai thudng khae nhau. Cdc hgp c h i t dae trung ehu y i u Id d eae loai thupc chi ndy Id (E)-cinnamyl aldehyd, camphor, eugenol vd safrol... nhung d i i vdi cay
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• N g h i e n CLFU - Ky t h u a t
re xanh {Cinnamomum tonkinensis (Lecomte) A.
Chev.) thdnh phin hea hge ehu y i u cinnamyl aldehyd. Day la mpt nguin tinh d i u mdi chira cdc hgp c h i t cinnamyl aldehyd va kh6ng the thay t h i bing eae san p h i m t i n g hgp.
K i t luan
Hdm lugng tinh d i u Id cay re xanh {Cinnamomum tonkinensis (Lecomte) A. Chev.) thu hdi d Hd TTnh la 0,75%. Bing phuang phdp s i c ky khi (GC) va s i c ky khi k h i i p h i (GC/MS), tip tinh d i u la cay re xanh {Cinnamomum tonkinensis) d Ha TTnh, han 40 hgp c h i t dugc tdch ra, trong do 31 hgp c h i t dugc xae djnh chiim d i n 95,2% cua t i n g ham lugng tinh d i u . Thanh phin chinh ciia tinh d i u la cinnamyl aldehyd (32,6%), p-phellandren (14,7%), a- pinen (12,5%), linalool (11,3%).
Summary
From Cinnamomum tonkinensis collected in Vu Quang National Pari< (Ha Tinh province, Vietnam) in Juanary 2010, the leaf essential oils were steam-distilled (yielding 0.75%) and analyzed by Capillary GC and GC/MS. Thirty one components were identified accounting for more than 95.2% of the oil. The major constituents were cinnamyl aldehyde (326%), ^phellandrene (14.7%), a- pinene (12.5%), linalool (11.3%); the minor group of the predominant compounds included f3-pinene (7.5%), 1,8-cineole (2.5%), p-ocymene (1.6%), myrcene (1.9%,), benzyl benzoate (1.6%,), ^ caryophyllene (1.4%), sabinene (1.1%,) and a- terpineol(1.1%).
Tai lieu tham khao
1. Wu Zhengyi & Peter H. Raven (eds), Flora of China, Vol. 7 Lauraceae, Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis. (2003). 60-86.
2. Fham Hoang Ho, Cay co Viet Nam, Q u y i n I, Nha xuat ban Tre, TP H6 Chi Minh (1999).
3. La Dinh M&i va mot s6 tac gia khac, Tai nguyen thuc vat CO tinh dSu a Viet Nam, Nha xuit ban Nong nghiep. Ha Noi (2001).
4. Lavy/rence B. M., Essential Oil as Sources of Natural Chemical. 12"" Int. Congerss of Fragrances, Flavors and Essential Oil, Sept/Oct., Australia, 16 (1992)24.
5. Nguyin Kim Dao, Danh luc cac loai thuc vat a Viet Nam, Nha xuit ban Nong nghiep, Ha Npi (2003), Tap 2, 73-82.
6. Dugc dien Viet Nam, Nha xuit ban Y hge, Ha Npi (1997).
7. Tran Dinh Thang, Hoang Van Luu, and Nguyen Xuan Dung, Chemical composition of the leaf oil of Canarium bengalense Roxb. from Vietnam, Joumal of Essential oil and Bearing Plants, 7(1) (2004) 43-48.
8. Heller S. R. and Milne G. W. A., EPA/NIH Mass Spectral Data Base, U.S. Govemment Printing Office, Washington D. C. (1978, 1980, 1983).
9. Stenhagen E., Abrahamsson S. and McLafferty F. W., Registry of Mass Spectral Data, Wiley, New York (1974).
10. Svi^igar A. A. and Siverstein R. M. , Monoterpenens, Aldrich, Milwaukee (1981).
11. Adams R. P., Identification of Essential Oil Components by Gas Chromatography/ Quadmpole Mass Spectrometry. Allured Publishing Corp. Carol Stream, IL (2001).
12. Joulain D. and Koenig W. A., The Atlas of Spectral Data of Sesquiterpene Hydrocarbons, E. B.
Verlag, Hamburg (1998).
T o n g q u a n v e . . . (nip theo trang 58)
Cong nghe, de dugc hudng cdc ehfnh sach uu dai hien hanh cho ede d i i tugng nay.
Cho phep Bp Y t i triin khai thi diem viec t i chirc cae loai hinh lien doanh lien k i t giQ'a cdc dan vi nhd nude va eae doanh nghiep trong viee thdnh lap cac Tmng tam thip tuang duang sinh hpc (BA/BE-Bbavailability/BioEquivalence Testing Centres) trong k i hoaeh khuyin khieh san xuit thuic g i c (Generic dmgs) de cung irng cho thuic Bao hiem Y t i va cdc chuang trinh thiu quie gia.
Bp K i hoach vd D i u tu chu tri p h i i hap vdi Bp Y t i , Bp C6ng Thuang, Bp Tai chinh vd eae Ban nganh lien quan khin truang ban hdnh eae ea e h i chinh sdch uu dai cu the cho cdc Du an d i u tu san xuit Vaccin vd Sinh p h i m Y t i v i :
30
gia k i hcaeh eua ede loai vaccin trong Chuang trinh tiem ehung md rong; ehinh sach v i t h u i cho nguyen lieu d i u vdc, v i vay v i n vdi lai suit uu dai; bao hp cae san p h i m d i u ra eua Du dn san x u i t vaccin sinh p h i m y t i .
Cho phep Bp Y t i phii hgp vdi cdc B6 nganh lien quan phat dpng eae Chiin dich "Ngwai Viet wu tien dung thudc Viet' (thuic duac d i u tu san xuit tai Viet Nam) trong cae ea sd kham vd diiu trj edng lap vd tu nhan tren pham vi ea nude, theo dung Nghj quyit eua Bp Chfnh tri; Ban hdnh ede Quy djnh phdp ly dp dung ehung cho ede Ca sd khdm chQ'a benh cdng lap v i viec uu tien xet thiu eho cdc san phim dat tieu chuin theo quy djnh va duac diu tw san xuit trong nwac.
TAP CHI DU^OC HOC - 9/2010 (SO 413 NAM 50)
