LKNG DUNG CUA NO TRONG CHE BIEN THUY NGHIEN CLFU TONG HQP DUNG DICH TRUNG PEROXOCACBONAT KHONG BEN VA TRIEN VONG

TAP CHI KHOA HOC VA C O N G NGHE Tap48, s6 2, 2010 Tr. 47-56

NGHIEN CLFU TONG HQP DUNG DICH S A T TRUNG

PEROXOCACBONAT KHONG BEN VA TRIEN VONG LKNG DUNG CUA NO TRONG CHE BIEN THUY S A N

N G O Q U O C BUU,

NGUVeN HOAl

CHAU, NGUYEN

VAN

HA

1. M O DAU

Hien nay trong linh vuc che bien thiiy san (CBTS) viec sir dung cae chat sat trung cd chiia clo bj kiem soat ngay cang nghiem ngat. Thang 5-2008 tai My, Td chiic Luong - Ndng Lien hiep qudc (UN FAO) da td chuc cude hdi thao qudc te ve cac chat khCi' triing chii'a clo nham danh gia lai cac dac tinh khii' trung cua ehiing [1]. Tai day eac nha khoa hpc da cho thay cac chat sat triing chiia clo cd the de lai du lugng tren san pham che bien, cd kha nang hinh thanh san pham phu halogen hu'u ea ddc hai tren be mat thjt ea, ma trude day khdng phat hien dugc do dp nhay ban che ciia phuong phap phan tich. Ngudi ta ciing da dua ra khuyen cao che tao cac chat sat trung mdi khdng chiia clo [ 2 - 5 ] .

Trong khi dd d nude ta hau het cac co sd CBTS cho den nay van su dung canxi hypoclorit Ca(0Cl)2 dang ran, cd ham lugng clo boat tinh tren dudi 10% de khu' triing nudc, dung cu, san pham va nha xudng. Tuy nhien, canxi hypoclorit do dp hda tan kem nen thudng de lai can tren san pham, ddng thdi trong qua trinh pha che thanh dung djch ^ khi clo thoat ra manh, gay d nhiem mdi trud'ng, lam anh hudng ' ^ ' ^ ^ den siie khde cdng nhan.

Trong linh vuc khii' trung, cae hgp chat hda hgc cd hoat tinh khir triing cao nhung cd ddc tinh thap ddi vdi ddng vat mau ndng va than thien vdi mdi trudng la cac dung dich nudc boat hda dien hda [6]. Cdng nghe boat hda dien hda (HHDH) dugc phat minh bdi kT su ngudi Nga V. M. Bakhir nam 1972 va nam 1984 dugc Vien HLKH Lien Xd cii thira nhan nhu mdt hudng phat trien mdi trong ITnh vuc dien hda iing dung. Trong khoang 1/4 the ki trd lai day cac thiet bj san xuat dung djch HHDH diing cho muc dich khir triing, cd ten ggi chung la thiet bj STEL, da dugc thuo'ng mai hda va iing dung rdng rai tai nhieu nudc tren the gidi, trong dd cd Viet Nam [7 - 11].

Hoat hda dien hda la mdt qua trinh dien hda trong dd dung djch nudc cd dp khoang hda thap [12], dudi tac dung ciia mpt dien trudng don cue trong budng dien hda ddng chay cd mang ngan luong cue (FEM-3, cac Hinh 1 va 3), dupe dua len trang thai kich thich gia ben vdi cac tinh chat hda - li dj thudng, thay ddi theo thdi gian, va tu phuc hdi vk trang thai can bing vdi thdi gian keo dai nhieu gid. Dung djch thu dugc tai ldi ra cua khoang andt dugc ggi la anolit, tai ldi ra tir khoang catdt la catolit.

Anot

M^ng ngan

Hinh 1. Buong dien hoa hoat hoa FEM-3

f

^

r h

1

0

^

^

1 0

^

2 0

^ Vi_

2

.3 0

^

4 0

-////.

5 0

p////

6 0,

c -a

Nguyen li boat ddng cua cac thiet bj HHDH kiSu STEL dua tren nguyen tic nay.

Vien Cac He thdng va Cdng nghe dien hda Vitold Bakhir (VCHTDH&CNVB, Hucmumyrn SjieKmpoxiLMunecKux CucmeM u Texuo.ioauu B. Eaxupa) thudc Bd Y te LB Nga la noi chuyen nghien ciiu va phat trien cac he thdng dien hda mdi, dac biet la cdng nghe HHDH. Tai noi day cac nha khoa hpc da thiet ke che tao nhieu loai thiet bj STEL khac nhau, trong sd dd thiet bj STEL-PEROX dugc xem la mdt phuong tien diet khuin huu hieu va than thien mdi trudng nhat nhd da tao ra dugc dung djch pecaebonat vdi boat tinh khang khuin dac biet cao nhung khdng chiia cac hpp chat clo ddc hai.

Diem co ban ciia quy trinh tdng hgp dung djch pecaebonat trong thiet bj STEL-PEROX la dung djch cacbonat diu vao cd ndng do rat thap (0,4 - l,Og/l), chi tuong duong do khoang hda cua nude an udng.

Hinh 2 chi ra su phu thupc dp dan dien eiia cac chat dien li vd co' khac nhau vao ndng dp ciia chiing trong dung djch nudc (dudng eong 1). 0 dung djch dien li loang (< 0,1 mol/1) mdt thay ddi rat nhd ve ndng do ctia chat tan cd the gay ra sir bidn ddi dang ke cua dp dan rieng, khang djnh su cd mat cua mpt lugng Id'n cac phan ttr nudc khdng tham gia vao qua trinh hinh thanh cac ldp vd hydrat bao quanh cac hat mang dien tich. Ddi vdi trud'ng hgp dung dich dien li cd ndng dp dam dac hon (> 0,1 mol/1) cd the thay nhirng bien thien Id'n ve ndng dp cung khdng lam cho dp dan dien eua dung djch thay ddi bao nhieu. Dieu do chiing td cac ldp vd hydrat bao quanh cac hat dien tieh bat dau tuong tac vdi nhau va miic dp xam nhap lan nhau giira ehiing tang theo sir gia tang ndng dp cua cac chat dien li. Y tudng nay dugc the hien bang dudng eong 2 tren hinh 2 trong dd cd the thay khoang each giiia cac tam ion cua cac chat dien li phu thudc vao ndng dp cua chiing trong dung djch, ddng thdi ndng dp cang tang, khoang each giiia cac ion giam cang it.

Dud'ng cong 2 dupe xem nhu mdt bieu thj dac thii cua tinh chat cau triic ciia dung djch dien li [13]. Khai niem "dung djch cau triic - structurized solution" bao ham tinh trat tu ben virng tuo'ng ddi theo thdi gian va khdng gian ciia cac vj tri ciia cac hat dien li va cac phan tu' nudc trong dung djch so vol vj tri cua mpt hat dugc chgn ngau nhien. Theo djnh de ndi tieng cua L.

Pauling ve tinh trung hda dien tich cila cae ion hydrat hda, thi dien tich cua mdt ion dugc tai phan bd lai tren cac nguyen tir hydro cua cac phan tir nude bao bgc xung quanh ion, va sir trung hda va tai phan bd dien tieh nay dien ra trong qua trinh hydrat hda ion. Sir tai phan bd dien tich cua ion cdn tac dpng den cac phan tu' nudc nam d xa hon. Nhu vay rd rang sir djch chuyen mpt phan mat dp dien tir cac phan tir nudc nam gan ion nhat se lam cho cac phan ttr nude nay bj thieu hut mat dp dien ttr, nhung sir thieu hut nay sau dd dugc den bu bang sir djch chuyen mat do dien tu cua cac phan tu nude diing sau, va cii the tiep tuc eho den khi cac hat ion hydrat hda dupe trung hda dien tich va trd thanh eac hat cd ciu tnic ben vung, dupe gpi la "aquacomplex".

Ddi vdi cac hat anion sir djch chuydn mat dp dien tich cung dien ra tuong tu nhung theo hudng ngugc lai. Nguyen nhan cua cac djch chuyin dd la xu hud'ng dat tdi trang thai can bang vdi nang lu'png cue tieu. Do vay sir phdi tri cae phan tu nudc xung quanh hat tan la yeu td quan trpng ddi vdi qua trinh ciu triic hda dung dich, cu thd la qua trinh hydrat hda cae hat tan.

Nong dQ, mol/1 "

Hinh 2. Dp din dien long quat ciia hau het cac chit dien li vo ca va khoang each giua cac ion

trong dung dich phu thuoc vao nong do cua chiing

Oj . Oj . HjO, H*. C,Oi"

TH2O

Trong qua trinh tdng hpp dien hda dung djch pecaebonat tii' dung djch cacbonat (hoac bicacbonat) loang dieu cue ky quan trpng la qua trinh hydrat hda cac hat tieh dien phai duge thuc hien tai viing sat bd mat dien cue, noi cud-ng do dien trud'ng ciia ldp dien kep dat gia trj hang tram nghin von/cm, nhim ciu true hda dung djch mdt each cd djnh hudng. Nhu vay cd the ndi, dp khoang hda thip eua dung djch dien li cd anh hudng quyet djnh ddn dp ben cua cac phiic phan tir aquacomplex vdi lien ket hydro duge tao ra trong qua trinh boat hda dien hda. So do nguyen li cua qua trinh tdng hgp dien hda dung djch pecaebonat dugc md ta tren Hinh 3. Dung djch cacbonat natri loang dugc cho chay qua khoang andt cua budng dien hda FEM-3, trong khi tai khoang catdt ddng nudc tinh khiet dugc cho ehay ngugc chieu. Luu lugng ddng nudc di qua khoang catdt phai dam bao ldn hon luu lugng dung djch nudc mudi di qua khoang andt nham thiet lap eac dieu kien ma tai dd ddng dien di qua mang ngan cua

budng dien hda dugc tao ra hoan toan bdi qua trinh van chuyen cation tir viing andt sang viing catdt. Ddng thdi ap suat du trong khoang andt (0,1 at - 0,5 at) ciing tao dieu kien thuan lgi cho qua trinh van chuyen cation va can trd cac anion hydroxyl xam nhap tir khoang catdt sang. Vdi ap suat < 0,1 at khdng the ngan chan cac ion hydroxyl chay ngugc tir khoang catdt sang khoang andt, do dd qua trinh dxy hda cac ion cacbonat trong viing andt bj ban che. Nhung khi ap suat >

0,5at, ngoai cation kiem, anion cacbonat tir khoang andt cd the bj day sang khoang catdt lam giam hieu suat tao cac hgp chat peroxo (tinh theo ddng dien).

t H2O. Na*.

OH". H2

Hinh 3. Sa do nguyen li tong hop dung djch percacbonat trong buong dien hoa

hoat hoa FEM-3

Catolit PEROX

NajCOs (Dung djch loang)

!

fi

jf .J Duong nuac

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Anolit PEROX

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Hinh 4. So do mach dien va thuy lire cua thigt bi STEL-PEROX thu nhan - dung djch anolit-PEROX tu dung dich cacbonat kigm loang

Tren ca sd cac modun FEM-3 dirge nhap to LB Nga va so dd cdng nghe do VCHTDH&CNVB cung cip, Vien Cong nghe mdi tnrdng da thidt ke chd tao thanh cdng thiet bj STEL-PEROX cho phep thu nhan dung djch peroxocacbonic (cdn dugc ggi la anolit -PEROX)

cd boat tinh khii trimg cao, than mdi trudng va tac dung keo dai. Hinh 4 trinh bay so dd mach dien va he thdng thiiy lire cua thilt bj STEL-PEROX.

Cac phan iing hda hgc co ban dien ra trong qua trinh dung djch cacbonat natri loang lan lugt chay qua cac khoang andt ciia 8 budng FEM-3 dugc mac ndi tiep nhau de cudi cung thu nhan dung djch percacbonat trung tinh cd boat tinh khu triing cao da dugc md ta chi tiet trong cdngbd [12].

Bao cao nay trinh bay ket qua nghien ciiu dieu che dung djch peroxocaebonat (anolit PEROX) tren thidt bj dien hda boat hda STEL-PEROX va trien vgng iing dung nd dd khir trimg trong che bien thiiy san.

2. PHAN THtrC NGHIEM Qua trinh tdng hpp dung djch anolit PEROX dugc thuc hien tren thidt bj STEL-PEROX do Vien CNMT thiet kd lip dat dugc the hien tren Hinh 5.

Phd hip thu ciia dung djch anolit PEROX dugc do tren may quang phd UV-VIS GBC Instument-2885.

2.1. Moi tru'O'ng nuoi cay vi sinh

- Cac dung djch sinh li dieu che tii' pepton (Merk);

- Chromocult va SSagar (Merk) lam mdi trudng de phan lap E.coli va Salmonella;

- Mdi trudng PCA (Merk) de phan tich djnh lugng vi sinh.

2.2. Thu thap mau vi sinh Hinh 5. Thiet bj STEL-

PEROX cong suit 201it/gid do Vien CNMT che tao Cac mau vi sinh thu thap to nudc nudi, cac be mat cdng cu

lao ddng va san pham trong xi nghiep che bien thuy san.

Boi tiegng khdo sdt: Muc ddng lanh, mat ban inox, ban da hoa.

Vi khuan khao sdt: TPC, coliform. Salmonella va E.coli - cac loai vi khuan thudng gap trong CBTS

2.3. Khao sat tinh nang sat khuan cua anolit-PEROX tren be mat cong cu lao dong va tren hai san

y mau in tich

Mirc dong lanh

Diing khan tam anolit lau

sach

— •

Lay mau phan tich ^^

•4— Riia nudc thudng ^•4—

Riia nudc thudng ^{— •}

Vat dau, boc vo, lot bo noi tang

mau phan tich

t

Trang len be mat inox, da

hoa

<—

• Xay

nhuyin ^{— •}

Bo sung E.

coli va Salmonella

t

Pha loang trong pepton, li 37°C 24 gid Hinh 6. Sa dd gay nhiim va liy miu phan tich vi sinh tren be mat ban inox va ban da hoa

Quy trinh gay nhiem va lay mau phan tich vi sinh tren be mat ban inox va ban da hoa dugc the hien tren so do khdi (Hinh 6). Tae nhan khu trung la dung djch anolit-Perox hoac anolit ANK [11].

2.4. Khao sat hoat tinh khang khuan cua dung djch anolit-Perox tren san pham hai san

Ddi tugng vi khuan khao sat van la tdng tap khuan, coliform. Salmonella va E.coli. Quy trinh xae djnh boat tinh khang khuan ddi vdi cac loai vi khuan neu tren dugc md ta tren Hinh 7. Tuy nhien, rieng ddi vdi trud'ng hgp phan tich djnh tinh Salmonella, 25g lugng can mau djch xay dugc pha trong 225ml djch Selenite Enrichment Broth Leifson.

3. KET QUA VA THAO LUAN

3.1. Cac thong so hoa-li cua dung dich anolit-PEROX

Mu'c dong lanh

i

Vat d^u, rau, lot bo noi tang

i

Rda sach Chia lam 3 phan deu nhau Rii'a nirfi'^rT^am A-Pcrox NgSnTTt^NK

^~~Xa^hiiy 4.

Can lg cho vao ong nghiem lOmI Pepton

4.

Phan tich vi sinh hoc

PTmh 7. Quy trinh khao sat hoat tinh sat khuan ciia dung dich anolit-Perox

va anolit ANK co ciing nong do clo boat tinh tren san pham hai san

Cac thdng sd kT thuat co ban cua thidt bj STEL-PEROX va cac chi sd h'-bda cua cac dung djch anolit va catolit thu dugc tren thidt bj duge trinh bay tren bang 1, trong dd cd the thay dp khoang hda ciia dung djch anolit PEROX thap, tuong duong dp khoang hda ciia nudc an udng.

Bdng 1. Cac thdng sd kT thuat cua thiet bj STEL-PEROX va cac chi sd li - hda ciia cae san pham hoat hda

Dung dich HHDH Anolit Catolit

[NajCOj], g/1 (nong do

ban dau) 12 H2O

Dong dien

(A) 20-21

Dien ap (V) 38-40

Cong suat

(1/h) 20-22 58-60

TDS (mg/l) 620-750

pH 6.6-6.7

9-10

The oxy hoa khu ORP

(mV) +700 - +800 +250 - +350

[Chat oxy hoa]

(mg/l) 60-65

Bdng 2. Bien thien ciia cac chi sd li-hda ciia dung djch anolit PEROX phu thudc vao thdi gian bao quan (trong can day nap kin)

Thdi gian bao quan (ngay) 1

2 3 4 5 8 9

pH 6,68 6,87 6,90 6,95 6,95 7,00 6,74

The dxy hda khii (mV) 786

770 771 754 748 726 710

[Chat dxy hda] (mg/l) 67,4

61,8 57,5 57,5 56,8 51,5 49,7

Bien thien ciia cae chi so li - hda cua dung djch anolit PEROX trong thdi gian bao quan 9 ngay trong binh day nip kin dugc chi ra tren bang 2. Co thd thiy ndng dp cac chit oxy hda va thd dxy hda khu eiia dung djch anolit PEROX suy giam tuong ddi cham sau 9 ngay bao quan (ORP giam 10%, trong khi ndng do cac chit dxy hda giam 25%).

Hinh 8 cho thiy anh hudng eua ddng dien len the dxy hda khu va dp khoang hda cua cac dung dich HHDH (anolit PEROX va catolit PEROX). Dang ehii y la khi ddng dien phan tang thi dp khoang hda cua anolit giam trong khi dp khoang hda ciia catolit tang.

Phd hip thu UV-VIS ciia dung djch anolit PEROX vira mdi dieu che va sau thdi gian bao quan duge din ra tren Hinh 9. Kdt qua cho thay sau 9 ngay giu trong binh kin tat ca cae thanh phin gia bdn vdi thdi gian sdng ngan da phan hiiy hoan toan va dinh hap thu cue dai dich chuyen ve phia budc sdng dai. Didu dd mdt lan nua khang djnh tinh chat gia ben cua dung djch HHDH.

1 1 900

^ 700 E 500

o ^°°

1

_^_^,...

I"

b))

^?'^^---ii-" ^ • - ^ . ^ » V . U . U . . L

^'"- —. ,

3 5 10 15 20 25 A

Hinh 8. Anh hudng cua dong dien dien phan len do khoang hoa va the oxy hoa khu cua anolit PEROX va catolit PEROX

200 250 300 330

Wavelength (nm)

•Anolit mai diSu chg; •-Anolit sau 9 ngay bao

Hinh 9. Phd hap thu cua anolit PEROX mai dieu che va sau thdi gian bao quan 9 ngay Kit qua khao sat thdi gian luu giir boat tinh ciia dung djch anolit PEROX chi ra rang qua trinh phan hiiy axit pecacbonic, nghTa la qua trinh suy giam boat tinh, dien ra cham: sau 30 ngay bao quan hoat tinh khang khuan cua dung djch chi giam khdng qua 20%. Su suy giam boat tinh nay thap hon nhieu so vdi cac dung dich anolit dugc dieu che tu' mudi NaCI tren thiet bj STEL [11, 13]. Sd dl dung djch anolit PEROX cd trang thai gia ben keo dai khae thudng la do qua trinh dien phan dung djch cacbonat kiem cyc loang da dupe thuc hien tai khu vuc can ke ldp dien kep, nhd dd cac chat dxy hda tao ra da dugc bao bpc bdi mdt ldp vd hydrat nhieu ldp dudi dang phiic qua trung hda dien tich.

3.2. Khao sat hoat tinh khang khuan ciia dung djch anolit PEROX

Dung djch anolit-PEROX da duge nghien ciiu boat tinh khang khuan tren mpt sd vi khuan gay benh thudng gap trong che biln thiiy san. Hoat tinh khang khuan in vitro cua anolit PEROX ddi vdi E.coli va Salmonella spp. dugc trinh bay tren bang 3. Ket qua cho thay sau 5 phiit tilp xuc vdi dung djch anolit-PEROX ndng dp 50 mg/l E.coli bj tieu diet hoan toan tren cac mlu huyen phu cd dp pha loang khac nhau, trong khi Salmonella bj vd hieu hda hoan toan sau 10 phut phoi nhiem.

Bdng 3. Hoat tinh kbang khuan in vitro cua dung djch anolit PEROX ([Cac chat dxy hda]

= 50 mg/l) ddi vdi ^.co//va 5'a/7«o«e//<7 d muc dp pha loang khac nhau

Mau thi nghiem

Mtfc do pha loang

10° 10' 10^ 10' 10" 10=

Nong do cac chat oxy hoa = 50 mg/l E.coli (cfu/ml)

Mau ddi chiing (khdng cd anolit)

Phoi nhiem 5 phiit Phoi nhiem 10 phiit Phoi nhiem 15 phiit

* 0 0 0

* 0 0 0

* 0 0 0

» 0 0 0

» 0 0 0

10^

Salmonella Mau ddi ehii'ng (khdng cd

anolit)

Phoi nhiem 5 phiit Phai nhiem 10 phiit Phoi nhiem 15 phut

*

» 0 0

*

» 0 3

*

» 0

1

» - 1 7 0 0

0 0

284 28

Khong dem dugc.

Bdng 4. Hieu qua khOr triing ciia dung djch Anolit-Perox tren san pham muc so sanh vdi dun^

djch anolit ANK cd ciing ndng dp clo boat tinh 50 mg/l

Mau

Ddi chirng (rua nudc thudng)

Ngam A.-Perox sau 15 phut Ngam A.-ANK sau 15 phiit

TPC cfu/g 1,9 X 10"*

3,2 X 10' 5,2 X 10'

E.coli cfu/g

<10

<10

<10

Coliform cfu/g

27

<10 10

Salmonella / 2 5 g

+ - - Hinh 10. Coliform a do pha

loang lO' trong moi truorig Chromocult ciia mau mu'c ngam

20 phiit trong Anolit-Perox 50 ppm (a) va ngam trong Anolit-

ANK 50 ppm (b) so sanh vdi mau doi chirng ngam trong

nuac may

Bang 4 the hien kha nang diet khuan cua anolit-PEROX tren san phim myc ciia Viet Nam.

sd lieu thyc nghiem dan ra cho thay mat dp vi khuin tren san phim myc da giam dang ke sau khi muc dugc ngam vao dung dich anolit PEROX ndng do 50 mg/l 30 phiit. Cu the la thanh phan TPC giam 9 lan, E.coli va Coliform - 2 lan trong khi Salmonella bj loai trir hoan toan (theo kit qua phan tich djnh tinh). So sanh hoat tinh khu triing eua anolit-Perox vdi anolit ANK cd cung ndng dp dan ra tren hinh 10 thay boat tinh cua chiing tuong duong nhau. Kit qua thu dugc chi ra rang ddi vdi cac chiing vi khuan khao sat (TTK, Coliform vd Salmonella) boat lyc diet kliuan cua dung dich anolit-Perox trong mpi trudng hpp la tuong duong so vdi dung djch anolit ANK.

Bdng 5. Ket qua khir trung mat ban inox bang anolit-Perox; cac mau vi sinh deu dupe lay sau 30 phiit tiep xuc

Mau 1 2 3 4 5 6 7 8 9

TPC cfu/cm^

2,0 X 10' 2,9 X 10' 2,5 X 10' 2,8 X 10' 1,0 X 10' 5,8 X 10' 2,6 X 10' 1,6 X 10' 8,0 X 10"

E.coli cfu/cm' 1,8 X 10' 4,6 X 10' 3,7 X 10'

0 4 2 0 0 0

Colifrom cfu/cm^

9,8 X 10' 2,0 X 10' 1,6 X 10"

2 15

4 0 0 0

Salmonella efu/cm^

3,7 X 10' 4,3 X 10' 3,2 X 10'

0 3 1 0 0 0

Ghi chu: Mau tir 1^-3: be mat ban inox dugc trang dich muc co bo sung them Salmonella va E. coli.

Mau tir 4->-6: be mat ban inox duoc trang dich myc co bo sung vi khuan va rira bang nudc may.

Mau tir 7-<-9: mau be mat ban inox dugc trang djch myc co bo sung vi khuan, riia bang nudc may va sau do lau sach bang khan nhiing anolit-Perox 60 ppm.

Bdng 6. Ket qua khir triing mat ban da hoa bang anolit-Perox; cac miu vi sinh diu dugc liy sau 30 phut tiep xiic

Mau 1 1 2 3 4 5 6 7 8 9

TPC cfu/em^

4,7 X 10' 2,7 X 10'

1,3 X 10' 1,3 X 10' 3,1 X 10' 3,5 X 10'

22 13 22

E.coli efu/cm' 4,0 X 10' 7,0 X 10' 2,1 X 10' 1,1 X 10' 2,3 X 10' 2,7 X 10'

0 0 0

Colifrom cfu/cm' 1,9 X 10"

2,9 X 10"

6,3 X 10' 4,4 X 10' 5,7 X 10' 4,0 X 10'

0 0 0

Salmonella efu/cm' 9,0 X 10' 8,0 X 10' 3,3 X 10'

9 25

4 0 0 0

Ghi chit: Mau tir l->-3: be mat ban da hoa dugc trang dich myc co bo sung them Salmonella va E.coli.

Mau tir 4->-6: bl mat ban da hoa dugc trang dich myc co bd sung vi khuan va riia bang nuoc may.

Mlu tir 7^9: mlu bl mat ban inox dugc trang djch myc co bd sung vi khuin, rira bang nudc may va sau do lau sach bang khan nhiing anolit-Perox 60 ppm.

Ket qua khir triing mat ban da hoa va inox trong xi nghiep CBTS duge dan ra tren bang 5 va 6 cbo thay E. coli, Coliform va Salmonella da bj tieu diet hoan toan sau khi bl mat ban inox cQng nhu ban da hoa rua bing nude may va sau do lau sach bang khan nhiing anolit-Perox 60 ppm. Ddng thdi ket qua cung cho thay ban inox de lam sach hon so vdi ban da hoa: eac mau 4 - 6 cua ban inox sau khi dugc rua bang nude may sd lug'ng E.coli, Coliform va Salmonella da giam gan bet, cdn trong trudng hgp ban da hoa thi cac loai vi khuin nay chi giam khoang 2 bac.

Ddi vdi tdng tap khuan su khac biet nay ciing tuong ty.

4. KET LUAN

Cac dac tru'ng h'-bda va boat tinh khang khuan cua dung djch pecaebonat thu dupe tren thilt bj STEL-PEROX dugc thiet ke lap dat tai Vien Cdng nghe mdi trudng tren ca sd cac mddun FEM-3 nhap tir LB Nga da dugc nghien eiiu. Sd lieu thyc nghiem thu dugc chi ra rang ndng dp khoang hda cua dung djch dien phan cang thap, dp ben eiia cac phiic peroxocaebonat hydrat hda (aquacomplex) cang tang do chiing cang dugc trung hda dien tich. Nhd vay boat tinh gia ben cua dung djch dugc keo dai dang ke: ORP chi giam khoang 10% va ndng dp cae chat dxy hda - 25%

sau thdi gian bao quan 9 ngay.

Kit qua nghien ciiu eho thiy thilt bj STEL-PEROX cd kha nang tao ra dung djch khdng dpc hai do khdng tao ra cac chat chiia clo, nhung cd hoat tinh khang khuan cao, cho phep tieu diet cac loai vi khuin gay benh thudng gap trong CBTS nhu E. coli, Coliform, Salmonella. Vdi mat dp 10* cfu/ml E.coli bj tieu diet hoan toan sau 5 phut tiep xuc vdi dung djch anolit PEROX ndng dp 50 mg/l va Salmonella - sau 10 phut tiep xiic.

Dung djch anolit-Perox dugc san xuit tren thilt bj STEL-PEROX, mac dii cd gia thanh cao hon anolit-ANK, nhung hiia hen la mdt giai phap hQu hieu de khu triing san pham CBTS cd yeu cau cao ve du lugng chat khu triing cdn lai tren san pham sau xu li.

TAI LIEU THAM KHAO

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5. Baxnp B. M. Hoa pea. npoc}). B. M. Baxnpa - 3jieKTpoxHMHHecKaa aKXHBauHH:

yHHBepcajiLHbiH HHCxpyMeHT sejienoii XHMHH. M . , HHCXHTyr SjieKxpoxHMHHecKHX

CHCTCM H TexHOJioPHH BHTOJiBfla Baxnpa, 2005, 176 c.

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3BOJiK3nHa coBepmeHCTBOBaHHa H anajTHS nepeneKXHB npHMeneHHa, McflHUHHCKHii anitiaBHT. BojibUHua (2) (2008) 52-58.

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Nguyin Van Ha, Nguyin Hda Chau Nguyen Minh Tuan - Nghien ciiu iing dung dung dich HHDH dl khir trung nude va dung cu trong san xuat tdm gidng. Tap chi Khoa hoc va Cdng nghe 46 (6A) (2008) 227-233.

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SUMMARY

ELECTROCHEMICAL SYNTHESIS AND PERSPECTIVES OF APPLICATION OF PEROXOCARBONATE SOLUTION FOR DISINFECTION IN FISHERY PROCESSING

ENTERPRISES

STEL-PEROX is the latest model among the series of well-known STEL devices which produce electroehemieally activated (ECA) solutions widely used for disinfection in many countries over world, including Vietnam. STEL-PEROX devices stand out as the most effective and environmentally-friendly means against microbes by generating activeted percarbonic acid from highly diluted sodium carbonate solution.

Physico-chemical and functional characteristics of ECA solutions generated on a STEL- PEROX device designed and set up in Institute of Envonmental Technology-VAST using Russian electrolytic moduls FEM-3 have been evaluated. It was shown that this device produces actually non-toxic solutions but highly effective disinfectants that destroy all forms of pathogenous microorganisms such as E. coli Coliforms, Salmonella ete.

The experimental data showed that the low general mineralization of the anolyte PEROX provides a high stability of the molecular complexes which are hydrated, transforming into electroneutral aquacomplexes. That means that the lower the concentration of ions in solution, the greater the stability of the aquacomplexes, hence the longer the bactericidal activity of the anolyte PEROX.

Bia chi:

Vien Cdng nghe mdi trudng,

Vien Khoa hpc va cdng nghe Viet Nam.

Nhdn bdi ngdy 12 thdng 6 nam 2009