JOURNAL OF SCIENCE OF HNUE Natural Sci., 2013, Vol. 58. No. 3. pp. 24-32 This paper is available online at http://stdb.hnue.edu.vn
NGHIEN CUU XAC DINH HAM L U O N G M O T SO THUOC KHANG SINH TRONG THUC PHAM BANG PHUONG PHAP
SAC KI LONG HIEU NANG CAO (HPLC)
Tran Thi Hong Van, Dao Nguydn Thu Ha, Tran Minh Dflc va Nguyen Quang Tuyen Khoa Hda hpc. Trudng Dai hpc Supham Hd Npi
Tom tat. Bai bao trinh bay quy trinh phan tich xac dinh ddng thdi ham Ifldng ba chat khang sinh amoxicilin, oxytetracycline, ofloxacin bang phfldng phap sac ki long hieu nang cao (HPLC). Ket qua nghien cflu cho thay cd the sfl dung phUdng phap HPLC de xac dinh ddng thdi ham Ifldng ba chit khang sinh amoxicilin, oxytetracyciine, ofloxacin trong thflc pham vdi dp chinh xac cao. Dfla tren cac dilu kien tdi Uu da khao sat, quy trinh phan tich dfldc flng dung de xac dinh ddng thdi ham lUdng amoxicilin, oxytetracycline, ofloxacin trong mpt sd mau thuc pham nhfl thit ga, thit ldn ban tren thi trfldng. Ket qua phan tich cho thly ham Ifldng amoxicilin va oxytetracyclin trong mpt sd mau thit ga va thit ldn ban tren ihi trfldng cao gip nhieu lan so vdi tieu chuan cho phep ddi vdi thflc pham.
TUkhda: HPLC, antibiotics, amoxicillin, oxytetracycline, ofloxacin.
1. Mol dau
Trong chan nudi, khang sinh (KS) dfldc sii dung vdi 3 muc dich: dieu tri benh, phdng benh va diing nhfl chit kich thich sinh trfldng. Tdy theo muc dich sii dung ma lieu Ifldng va phfldng thflc su: dung KS cd khac nhau. Viec sut dung KS khong dung each trong chan nuoi din den mpt hau qua rat nghigm trpng la lam tang hien tfldng khang khang sinh cua cae Ioai vi khuan gay benh tren ngfldi va vat nuoi [ I -3]. Hien nay, tinh trang sfl dung thudc khang sinh trong chan nudi d Viet Nam rat khd kie'm soat. Ngfldi nong dan vi mudn tang nang suit va san Ifldng da sii dung khang sinh tran lan, khong dung chi din, lieu Ifldng,... din den dfl Ifldng thudc khang sinh trong eac san pham chan nuoi qua ldn, vfldt gidi han an toan cho phep [4, 5]. Cac chat khang sinh tdn dU lai trong cac san pham chan nuoi nhfl thit, trflng. siia,... se gay tac hai cho ngfldi tieu dimg. Lfldng khang sinh nay se tich tu dan trong cd the' va con ngfldi se rat dd bi "nhdn" thudc. mat kha nang chdng choi vdi benh tat [4-6]. Vi vay, viec phan tich, xac djnh chinh xac ham Iddng cac loai thudc
Ngay nhan bai: 22/4/2013. Ngay nhan dang: 10/6/2013.
Tac gia lien lac: Trin Thi Hong Van, dia chi e-mail: [email protected]
Nghien cifu xdc dinh hdm lupng mpt so thudc kbdng sinh trang thifc phdm...
khang sinh trong cac san pham ehan nudi la vd cung quan trpng va co y nghia ldn ddi vdi vin dg an loan thflc pham.
Trong bai bao nay chung tdi trinh bay quy trinh phan lich dinh Ifldng mpt sd thudc khang sinb (amoxicilin, oxytetracyclin, ofloxacin) thfldng dfldc sfl dung trong thflc an chan nudi bang phfldng phap sac ki long hicu nang cao (HPLC) [7-9| va flng dung dc xae dinb biim Ifldng cac thudc khang sinh nay trong thflc pham, chii yeu la trong thit gia sue va gia cam.
2. Noi dung nghien cii'u 2.1. Thtfc nghiem
* Hda chdt
- Cac chli chuan: amoxicilin, oxylclracyclin, ofloxacin dcu la chiit chuan tinh khiet cua Vicn kiem nghiem - Rp Y tc.
- 'Hit ca cac hoa chit va dung moi sfl dung trong nghign cflu ddu dat dp tinh khidl phan tich.
* Thiet hi
- May sac kf Idng hicu nang cao cua hang Shimadzu (Nhat Ban), cpl ODS C18, detector bap thu tfl ngoai UV-VIS, bdm cao ap SPD 20A.
- Bp loc dung moi va bp Ipc mlu vdi dfldng kinh 16 mang Ipe 0,45 //.m.
- Can phan tich Startorius co dd chinh xac 1 0,1 mg ciia Dflc - May pH met TOA pH METER HM - 16S cua Nhat Ban
* Quy trinh lay vd xd li mdu thit gd vd thit Uln - Ldy vd xi^ If .so hp mdu
Thit ga va thit Idn dfldc mua tai cac chd tren dia ban Ha Npi, dfldc xay nbuygn va bao quan trong lu Ianh d -18 "C.
- Quy trinh xd If mdu
+ Can chinh xac 10,0 g mau da dfldc xay nhuyen cho vao binh eelen.
-I- Thgm 15 mL dung dieh axetonitril, nghidn va lac dcu irong 2 gid. Lpc lly dich irong sang cdc thuy tinb. Lap lai bfldc nay 2 lln nfla dc cd ibc chidt dfl Ifldng khang sinb trong mau vdi hicu suit tdi da cd the.
- Lain sgch dich chief
+ Sii dung cpt silica gel de lam sach dich chiet. Hoat hda cdl lan Ifldt bang 5 mL metanol va 5 mL nfldc ell. Loai bd dung dich chay qua col.
-I- Cho dich chidt chay qua cpt. Ttrong giai doan nay, cac khang sinh trong mau se dflde h i p thi Ien be mat cac hai rin chfla trong cpt. Didu chinh cho dich nay chay ra khdi cpt vdi tde do cham, t i ^ g gipt mot.
-I- Riia cpt bang 5 mL nfldc cat 2 lln, loai bd dich chay ra khoi cot, lam kho cot.
Tiep theo riia giai bang 10 mL metanol vdi tdc dp cham, tiing gipt mot. Thu djch ra khoi
TrSn Thi Hong Van, Dao Nguyin Thu lia, Tran Minh Dilc va NguySn Quang Tuyen cot, CO vS duai 1 mL roi dinh mile vao binh I mL bang metanol.
2.2. Ket qua va thao luan
2,2.1, Khao sat dicu kicn dinh luong dong thbi ba chat khang sinh bang phuang phap HPLC
Qua nghien ciiu lfnh chai li hoa ciia 3 chk khang sinh (amoxicilin, oxylclracyclin, ofloxacin), ket hop vdi cac dicu kicn trong tai lieu Iham khao vii didu kicn co san cua phong Ihi nghiem, chung loi Ivta chon kieu sac kf phan bo pha dao, sijt dung cot silica CIS.
Trong qua irinh Ihttc nghiem chiing toi da lien hanh khao sal cac didu kien sac ki nhiT: lua chon pha dong, van t6c dong, budc song phal hicn, the tich tiem.
* Khdo sdt lUa chpn pha dpng
Chiing toi dii ticn hanh khao sal mSu chuan h6n hdp 3 loai khang sinh trong ciing didu kicn sac kf: toe do dong la 0,75 mL/phul, the tich ticm la 10 /^.L tren he pha dong vdi thanh phSn gdm metanol - axit oxalic - acelonilril vdi cac ti lc khac nhau, lai A = 254 nm.
Ket qua khao sat cho lhay: vdi pha dong la metanol - axil oxalic - axelonilril theo ti le 20:
55: 25 {%), sac ki dd cho hinh dang pic gpn, Ihdi gian luu vila phai. Vi vay chung tdi chon pha ddng la melanol - axit oxalic - axetonitril vdi ti lc 20: 55: 25 (%).
* Khdo sdt lua chpn budc sdng
De lua chpn budc sdng thich hdp phat hicn ddng thdi 3 loai khang sinh, chiing toi tien hanh quet phd UV cua cac dung dieh chuan rieng re eiia tUng khang sinh tren dai sdng 190 - 400 nm (Hinh 1, 2 va 3).
Hinh I. Pho hap thu UV cua oxytetracyclin d ln~ 4,928 phut
Htnh 2. Pho-hdp thu UV cia amoxicilin d In = 2,604phiit Ket qua cho IhSy budc sdng hap thu cUc dai lan lUdt la: amoxicilin - 231 nm;
oxytetracyclin - 269 nm va ofloxacin - 297 nm. TO kdt qua quet phd' UV, chiing tdi nhan thay can phai lUa chpn mot budc sdng trung gian sao cho dp hap thu cua ca 3 loai khang sinh ddu IUdng ddi ldn dc ket qua phan tich cd dp nhay cao. Tai budc sdng A = 240 nm do hap Ihu ciia cii 3 khang sinh deu tUOng ddi Idn; dudng ndn thang, ca 3 pic xuat hien vdi Ihdi gian luu phCi hpp, cac pic ddu, can xiJng (Mnh 4). Vi vay budc sdng dupe chpn de nghien ciJu dinh lupng hdn hpp 3 khang sinh tren la 240 nm.
26
Nghien cihi xde dinh hdm lupng mdt sd thudc kbdng sinh trong thi/c phdh...
Hinh 3. Phd hdp thu UV cua ofloxacin dtp = 1,736phiit
Ilinh 4. Sdc kido cua hon hdp ofloxacin, amoxicilin, oxytetracyclin d bUdc sdng 240 nm
* Khdo sdt lUa chpn toe dp dong
Chung tdi ticn hanh chay sac ki cua dung dich mlu chua'n hdn hop d cac tdc dp khac nhau. Kdt qua cho thly vdi tde dp dpng 0,75 mL/phut cac pic dfldc tach tdt, ap suit cpt vfla phai va thdi gian Iifu ngin. Vi vay chung toi chpn tdc dp dong la 0,75 mL/phut de nghien cflu dinh Ifldng cac chat.
Tlf cae kdt qua khao sat, chiing tdi da xay dflng quy trinh sic ki co kha nang phan tich tdt cac khang sinh tren nhfl sau: Cdt tach; cot silica C18 (5 fim, 4,6 cm x 150 em);
Pha dpng: metanol - axit oxalic - axetonitril theo ti le 20%: 55%: 25%; Tdc dd dong: 0,75 mUphut; The' tich tiem: 10 //L; Detector UV: bfldc sdng 240 nm; Nhict dp pban tfch: nhiet dp phong.
Qua thflc nghiem vdi didu kien sac ki nhfl trgn, 3 chit dfldc tach ra hoan toan, cac pic can ddi va gpn, thdi gian Iflu ciia 3 chit hdp li dam bao tbdi gian phan tich.
2.2.2. Danh gia phiidng phap dinh lifdng - Chudn bi hdn hpp dung dich chudn:
Pha hdn hdp dung dich chuan gde chfla ddng thdi cac boat chit amoxicilin, oxytetracyclin, ofloxacin: Can chinh xac 0,0367 g amoxicilin; 0,0323 g chit chuan oxytetracyclin va 0,0418 g ofloxacin rieng re. Hoa tan oxytetracyclin va ofloxacin trong dung dich HCI 0,01 M; boa tan amoxicilin trong dung dich H3PO4 pH ^ 3,5; trpn deu hdn hdp rdi rdt vao binb dinh mflc 50 mL, dinh mflc tdi vach bang nfldc cat hai lln.
- Khdo sdt tfnh thich hpp cua he thong sdc ki:
De danh gia mot phep dinh Ifldng bang HPLC mdi xay dflng, trfldc het ta phai tidn hanh khao sat tinh thi'ch hdp cua he thdng sic ki. Vice khao sat tinh thich hdp cua he thdng sac kf dfldc tidn hanh qua viec tigm lap lai 7 lln dung dich hdn hdp chuan dfldc ehuan bi nhfl d phin 2.1. Ket qua tfnh toan cho thly cac dai Ifldng dac trflng nhfl: sd dia li thuydt (N), he sd bit ddi (AF) va dp phan giai (Rs) cua ba pic deu nam trong gidi han cho phep. Vay kha nang tach ba chit ciia he thdng sac ki cd tinh thich hdp tdt. Yeu cau ve dp lech chuan tfldng ddi ndi chung thlp hdn 2% la chip nhan dfldc. Tfl ket qua tren cho thay, RSD% ciia thdi gian Idu, dien tfch pic, dp phan giai gifla 2 pic ddu nhd hdn 2%, chflng to 27
Trin Thi Hong Van, Diio NguySn Thu Ila, Tran Minh Oflc va Nguygn Quang Tbydn he thdng sac ki cd do dam bao linh thi'ch hdp ddi vdi ca ba thanh phan phan tich.
2.2.3. Khau sat do tuyen tmh cua phu'ong phap va \ay difng diTcfng chuan - Khdu sdt khodng iwng dt) luyen ifnb
Chung ldi khao sat sfl phu thupc luydn U'nh giiia ndng dp cac chit can dinh lfldng vdi dien tich pic tren cac chat chui'n amoxicilin, oxytetracyclin va ofloxacin.
Ket qua khao sat cho thly khi ndng dp ciia amoxcicilin bien thien trong khoang 0,0188 > 1,2040 mg/mL, ciia oxytetracyclin bien thien trong khoang 0,0215 > 1,3760 mg/mL va ciia ofloxacin bidn thign trong khoang 0,020 > 1,280 mg/mL, co sif tfldng quan tuyen tfnh gifla ndng dp cac chat vdi dicn lich pic IUdng flng the hien qua cac phfldng trinh hdi quy vdi cac he sd Ifldng quan dcu rat gin 1. 'Iren cd sd dd, chung tdi chpn ndng dp dinh Ifldng cac khang sinh Irong mau nam trong khoang tuydn linh da khao sat.
- Xdy dung duifng chudn
Dfldng chuan ciia eac chit khang sinh (amoxicilin, oxytetracyclin va ofloxacin) dfldc bicu didn trgn cac Hinh 5, 6 va 7 bang phin mdm Microsoft Excel.
Hinh 5. Dudng chudn cda amoxicilin Ilinh 6. Dudng chudn cda oxytetracyclin
NDngdolmj/oill
Ilinh 7. Dudng chudn cua ofloxacin
Dg kicm tra dp chinh xac ciia dfldng chuan da xay dflng, chung toi pha dung dich chuan cua eac khang sinh d hai ndng dp khac nhau. Bam tifng dung dich vao thiet bi IIPLC cho may chay dung thdi gian luu se xult hien pic sac ki, ghi kdt qua dien ti'ch pic;
lap lai 7 lan. Diing dfldng ehul'n da xay dflng va thay dien tfch pic vao phfldng trinh dflcJng 28
Nghien cihi xdc dinh hdm lupng mat sd ihudc khdng sinh irong ibuc phdm...
ehul'n xac dinh dfldc ndng dd C,,. So sanh C, da tim dfldc theo dfldng ehul'n vdi gia tri thflc da pha vdi dp thdng kc cho trfldc de kid'm tra dp chfnh xac cua dfldng chua'n thu dfldc.
Dp chi'nh xac ciia dfldng chui'n dfldc danh gia qua gia tri dp lech chuan S va dp lech chua'n tfldng ddi RSD (%), Kdt qua thu dfldc cho thly RSD (%) dcu thlp dfldi 2% chflng td dfldng chuan cd dp chfnh xac cao gifla cac lln phan lich.
2.2.4. Khao sat do lap lai va do dung cua phu'dng phap
Chung tdi ticn hanh khao sat d 3 mflc ndng dp, d moi mflc ndng dp tidn hanh dp 6 lln de khao sat dp lap lai eua difin tich pic, sau do danh gia dp phan tan cua sd lieu dfla vap gia tri dp lech chuan S va dp lech chuan tfldng ddi RSD (%). Ket qua khad sat ehd thly, dp lech chuan tfldng ddi RSD% deu thlp va sai sd vdi cac infle ndng dp cua cac khang sinh la tfldng ddi nho. Nhfl vay, vdi chfldng trinh sle ki da chon. phfldng phap dinh Ifldng ba khang sinh co dp lap lai cao.
Dp dung ciia phfldng phap dfldc xae dinh bing phfldng phap them chuan (them khdang 20%). Thgm vao mau thfl mpt Ifldng chit chuan sao cho tdng ndng do cua chiing vin nam trong khoang tuyen tinh da khao sat. Tidn hanh chay sic ki dung dich chuan, dung djch ihii va dung dich them chuan, dfla vad dien tfch pic eiia cac dung dich nay linh ti Ie thu hdi Ifldng chit chuan thgm vao theo cong thflc:
H(%) = § (I) trong dd: C la ndng dp tim Iai trung binh, Co la ndng dp chuan them vao nen.
Ddi vdi mdi khang sinh, ticn hanh lap lai thflc nghicm 5 lln d 3 mflc ndng dp thap, trung binb va cao (Bang I).
Bang, Ch3t khang
sinh
Amoxicilin
Oxytetracyclin
Ofloxacin
. Ket gud xdc dinh do dting cua 3 khdng Nong do chuan
them vao nen (mg/mL) 0.037625 0,1505
0,602 0,043 0,172 0,688 0.04 0.16 0,64
Nnng do tim lai trung
binh (mg/mL) 0.037326 0,151374 0,605758 0,0428 0,1719 0.6853 0,0404 0,15995 0.6399
sinh tren thiil bi HPLC Do lech chuan tuong doi RSD
(%)
1.132 1.020 0.830 1.793%
0,887 0.433 1,025 0,731 0,283
He so thu hoi H (%) 99.21 100,58 100,62 99,57 99,93 99,61 101,00 99,97 99.99 Kdt qua cho thly, d lc thu hdi trung binh eua moi khang sinh ddu cao: amoxicilin 99,21% - 100,62%, oxytetracyclin 99,57%- 99,93% va ofloxacin 99,97% - 101,00%. Mat khac cac gia tri dp lech chuan tfldng ddi ciing nhfl sai sd tfldng ddi ddu thap, nhd hdn 2%.
Nhfl vay phfldng phap da khao sat dat yeu clu ve dp diing.
29
Trim Thi Hflng Vfli). Dim NguySn Thu llii. Tran Minh DUc vil'NguySji Quang Tuyen 2.2.S, Xac dinh ham Itfdng cac chai khang sinh Irong thtfc pham
* Doi llidng nghien ciiu
Cac mSu Ihuc phiim dupc nghien ciiu la cac niSu thit ga va thit Ipn dupc ban tai cac chd d thanh pho lia Npi. MSu dupc xii li va lam sach, ticp dd dupc lpc qua mang Ipc 0,45 lim. Mau sau khi xii li dupc lien hanh chay Ircn Ihict bj HPLC theo cac didu kien phan lich da chpn. Xac dinh va ghi lai gia trj dien tich pic Ircn sac ki dd se tinh dUdc nong do ba loai khang sinh trong mSu tii phUPng Irinh dudng chuan; lii dd linh dupe ham lupng ba loai khang sinh Irong miiu thit ban dflu theo cong thiJc:
C = C,„xli«« (2,
111
trong dd: C la ham Ifldng tifng khang sinh trong mlu thit ga hoac thit Idn ban dlu (mg/kg);
C,„ la ndng dp iflng khang sinh trpng dung dich mlu sau khi xii If (mg/mL); m la khdi Ifldng mau phan tfch.
1= Xdc tlinh hdm lUOng khdng sinh trong cdc mdu thit gd vd thit lan Mdu I: Mau thit ga mua ngay 12/10/2011 lai chd Nghia'Ian (xult xfl: trai chan nuoi ga Sdn Tay). Kdt qua phan tich cho thly trong Mdu I chi cd amoxicilin vdi ham Ifldng xae dinb theo phfldng phap dfldng chuan la 4,155 mg/kg, so vdi ticu chuan cho phep TCVN (0.2 mg/kg [ 1 ]) cao gip khoang 20 lan. Hdn nfla, sac kf dd eua Mdu I cho thay mlu cd nhidu tap chil cd the gay nhidu ket qua phan tfch. Vi thd, chting tdi tidn hanh phan tich mlu theo phfldng phap thgm ehuan dd loai trfl cac anh hfldng cua nen, cho kdt qua phan tich chinh xac hdn.
De ticn hanh pban ti'ch theo phfldng phap them chuan chiing toi chuan bi mpt day 4 dung dich chuan amoxicilin cd ndng dp khac nhau. Lan Ifldt them vao mdi dung dieh chuan ndi tren 50 //.I dung dich Mdu I. Sau dd ticn himh chay sac ki4 dung dich thu dfldc.
Tfl kgt qua thu dfldc chung tdi bicu dicn sfl phu thupc ciia dicn tich pic vap nong dp amoxicilin thgm chuan bing phin mem Microsoft Excel, thu dfldc dfldng them chuan.
Tfl phfldng ninh dfldng them chuan cd dang ij -- u.r |- /; chung toi tinh dfldc ndng dp amoxicilin trong mlu (:r - - ) la 0,02948 mg/mL- tfldng flng vdi ham Ifldng amoxcicilin
a
trong mau thit ga ban dlu la 2,948 mg/kg (theo eong thflc (2)).
Mdu 2: Mlu ihit ga mua ngay 19/10/2011 tai chd Cdng Vi (xuat xfl: trai chan nuoi ga Sdn Tay). Kdl qua xac dinh ham Ifldng 3 chat khang sinh trong Mdu 2 theo phfldng phap dfldng chuan cho thay Irong Mdu 2 col loai khang sinh la amoxicilin va oxytetracyclin.
Ham Ifldng amoxicilin trong mau la 8,431 mg/kg. so vdi tieu chuan TCVN cho phep (0,2 mg/kg) cao gip hdn 40 lan; ham Ifldng oxytetracyclin la 5,200 mg/kg, so vdi ticu chuan TCVN cbo phep (0,2 mg/kg) cao hdn gip khoang 25 lln. Sac ki dd ciia Mlu 2 cho thly mlu cd nhicu tap chit cd the gay nhicu kdt qua phan tich. Vi thd, chiing toi tidn hanh phan lich mau theo phfldng phap thgm chui'n dc loai trtf cac anh hddng cua ndn, cho kdt qua phan tich chinh xac hdn.
Bang phfldng phap them chuan chiing tdi xac dinh dfldc ndng dp amoxicilin trong 30
Nghien cifu xdc dinh hdm tupng mdt .\d ihudc khdng xinh trong ihuc phdm...
Mlu 2 la 0,07958 mg/mL - tfldng iing vdi ham Ifldng amoxicilin tidng mau thil ga ban dau la 7,958 mg/kg va ndng dp oxytetracyclin la 0,04799 mg/mL - ifldng flng vdi ham Ifldng oxytetracyclin trong mau thit ga ban dau ta 4,799 mg/kg.
/t^flwi.Mau thit ga mua ngay 22/11/2011 tai chd Nghia Tan (xult xfl: trai chan nudi ga c l u Didn). Ket qua phan tfch theo phfldng phap dfldng chuan cho thly trong Mdu 3 ehi ed I khang sinh la oxytetracyclin vdi ham Ifldng la 6,880 mg/kg, so vdi ticu chuan chd phep TCVN (0,2 mg/kg) cao hdn gip khoang 35 lln. Sac ki dd cua Mdu 3 eiing cho thly mlu cd nhicu tap chat cd the gay nhieu kcl qua phan tfch. Vi ihd, chung toi tign hanh phan tfch mlu theo phfldng phap them chuan dc ed kcl qua pban tich chinh xac hdn.
Bang phfldng phap them chuan chung tdi xac dinh dfldc ndng dp pxytetracyclin trong M l u 3 la 0,04947 mg/mL - tfldng flng vdi ham Ifldng oxytetracyclin trong mlu thit ga ban dlu la 4,947 mg/kg.
Mdu 4: Mau thit Idn mua ngay 19/10/2011 tai chd Nghia Tan (xult xfl: trai chan nudi Ung Hda). Ket qua phan tfch theo phfldng phap dfldng chuan cho thly irdng Mdu 4 cbi cd 1 khdng sinh la amoxicilin vdi ham Ifldng la 2.195 mg/kg, so vdi tigu chuan eho phep TCVN (0,2 mg/kg) cao hdn gip khoang 10 lln. Siie ki dd cua Mdu 4 cung cho thay mau cd nhicu tap chit eo the gay nhicu kdt qua phan tfch. Vi thd, chung toi tidn hanh phan tich mlu theo phfldng phap them ehuan de Ioai trfl cac anh hfldng eua nen.
Ndng dp amoxicilin xac dinb bing phfldng phap them chui'n la 0,01963 mg/mL - tfldng flng vdi ham Ifldng amoxcicilin trong mlu thit ldn ban dlu la 1,963 mg/kg.
So sanh kdt qua phan tich thed 2 phfldng phap, chung tdi nhan thly phfldng phap them chuan cho kdt qua chinh xac hdn so vdi phfldng phap dfldng chuan do loai bd dfldc cac ydu td anh hfldng eua nen mau. Trong mpt sd trfldng hdp, sfl sai lech cua phfldng phap dfldng chuan so vdi phfldng phap them chuan la khdng dang ke, nam trong gidi ban eho phep (< 15%) thi cd the chap nhan kdt qua xac dinh theo phfldng phap dfldng chuan.
3. Ket luan
Tidn hanh thflc nghicm trgn thict bi sac ki Idng hieu nang cao (HPLC) dc xay dflng phfldng phap dinh lUdng ddng thdi cac khang sinh amoxicilin, oxytclraeyclin. dfloxacin trong eac mlu thflc pham, chung toi da Ihu dfldc cac kdt qua sau:
- Co the xae djnh ddng thdi cac khang sinh amoxicilin, oxytclraeyclin, ofldxacin trong eac mau thflc pha'm bang phfldng phap sic kf long hieu nang eao (HPLC) dat dfldc dp chinh xac cho phep.
- Kdt qua khao sat dp tuyen tinh cho thly cd sfl tfldng quan tuyen tinb chat che gifla ndng dp va dicn tfch pic cac boat chit, cho phep ap dung quy Irinh phan tich dc xac dinb bam Ifldng cac khang sinh trong cac mau thflc pham vdi cac ti le ham Ifldng khac nhau.
- Ap dung phfldng phap HPLC phan tfch mot sd mau thflc pham da phat hicn mau thit ga, thit ldn ban tren thi irfldng cd chfla chit khang sinh amoxicilin va oxytetracyclin cao hdn ticu chuan cho phep nhidu lln.
Tran Thj Hong Van, Diio Nguyen Thu Ha, Tran Minh Dlic vii NguySn Quang Tuyen TAI LIEU THAM KHAO
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ABSTRACT
The quantitative determination of the presence uf three antibiotics in chicken and pork using High Performance Liquid Chromatography (HPLC)
In this study, a simultaneous quantitative determination of three antibiotics (amoxicillin, oxytetracycline and ofloxacin) was made using the High Performance Liquid Chromatography (HPLC) technique. The results show that an HPLC analysis can be used to determine the content of amoxicillin, oxytetracycline and ofloxacin present in food simultaneously and with high precision and accuracy. Under optimal conditions, Ihc process was applied to determine, simultaneously, the content of amoxicillin, oxytetracycline and ofloxacin present in chicken and pork meat that was being sold in the marketplace. The results showed that the content of both amoxicillin and oxytetracycline found in the some samples of chicken and pork meat were much higher than allowed by law.
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