Correlation between fatty acid profile and anti-inflammatory activity in common Australian seafood by-products. Microalgae have emerged as a source of bioactive molecules such as glycolipids with potent anti-inflammatory properties.
Results
Analysis of IL-10 production revealed increased levels in the TPA group compared to sham (p<0.05). Pretreatment with MGDG cream (100 mg per site containing 200 μg MGDG) attenuated the formation of macroscopic lesions (Figure 5a) and significantly reduced skin edema (p<0.001) when compared to the cream-TPA group (Figure 5b).
Discussion
On the contrary, the deposition of the fluorophore from lipid ointment on the skin was very low. The distribution of the drug in the internal oil phase (higher logP) is due to the hydrophobic characteristic of the drug.
Materials and Methods 1. Glycolipids
A specific piece of skin was cut and used for the permeation study after washing with distilled water. MGDG cream formulation (100 mg per site, containing 200 μg of MGDG dissolved in ethanol at 10 μg/μL), Dex cream (100 mg per site, equivalent to 200 μg of compound dissolved in ethanol at 10 μg/μL ) or with a comparable ethanol volume cream) were applied to the shaved skin of the back of the animals on an area of 1 cm2 using a syringe.
Conclusions
Role of PKC/ERK1/2 signaling in the anti-inflammatory effect of the tetracyclic triterpene euphol on TPA-induced skin inflammation in mice.Eur. The role of various inflammatory and tumor biomarkers in the development of carcinogenesis associated with ulcerative colitis.
Fucoxanthin-Containing Cream Prevents Epidermal Hyperplasia and UVB-Induced Skin Erythema
Introduction
Effects of fucoxanthin (FX) on pro-inflammatory cytokine production in THP-1 macrophages and HaCaT keratinocytes. Figure 4 shows that the results of mice pretreated with Dex confirmed the anti-inflammatory effect of this corticoid (p<0.001).
Materials and Methods 1. Cell Culture
Finally, the in vitro anti-inflammatory, antioxidant and protective activity of FX was confirmed in human keratinocytes. Molecular characterization and anti-inflammatory activity of galactosylglycerides and galactosylceramides from the microalga isochrysis galbana.J.
Protective Effects of Fucoxanthin on Ultraviolet B-Induced Corneal Denervation and Inflammatory
Discussions
In the present study, we showed that oral pretreatment with fucoxanthin promoted Nrf2 expression. Activated neural cells in the trigeminal ganglia and eye-swiping behavior were also reduced and UVB-induced inflammatory pain was suppressed in fucoxanthin-treated groups.
Materials and Methods 1. Experimental Animals
Otherwise, pp38 signaling pathways are associated in the regulation of Nrf2-mediated cytoprotective effect [51]. Inhibition of ultraviolet B-induced expression of the proinflammatory cytokines TNF-α and VEGF in the cornea by fucoxanthin treatment in a rat model.Mar.
Fucoxanthin and Rosmarinic Acid Combination Has Anti-Inflammatory Effects through Regulation of
HaCaT Keratinocytes
Materials and Methods 1. Compounds
Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes. Biochem. Rosmarinic acid inhibits nicotine-induced C-reactive protein production by inhibiting the activation of the NLRP3 inflammasome in smooth muscle cells. Antioxidant and anti-inflammatory effects of shungite against skin damage by ultraviolet B irradiation in hairless mice. Oxid.
Chlorpyrifos induces the NLRP3 inflammasome and pyroptosis/apoptosis via mitochondrial oxidative stress in human keratinocyte HaCaT cells. Toxicology.
Frondanol, a Nutraceutical Extract from Cucumaria frondosa, Attenuates Colonic
Materials and Methods 1. Chemicals and Reagents
- ELISA
Costastaxanthin inhibits colitis and colitis-associated colon carcinogenesis in mice via modulation of the inflammatory cytokines.Chem. Dextran sulfate sodium-induced inflammation is enhanced by intestinal epithelial cell chemokine expression in mice.Pediatr. Preventive and therapeutic effects of blueberry (vaccinium corymbosum) extract against dss-induced ulcerative colitis by regulation of antioxidants and inflammatory mediators.J.
Perilla frutescens extract ameliorates dss-induced colitis by suppressing proinflammatory cytokines and inducing anti-inflammatory cytokines.Am.
Correlation between Fatty Acid Profile and
Anti-Inflammatory Activity in Common Australian Seafood by-Products
Therefore, the by-products of the seafood industry can be a source of high-quality anti-inflammatory fatty acids. The anti-inflammatory activities of lipid extracts from predatory molluscs remain to be tested. The main ingredients of the well-known anti-inflammatory nutrient Lyprinol® and Biolane®, lipid extracts of the New.
The commercially available marine nutraceuticals Lyprinol® (BLACKMORES®, Alexandria, Australia) and Deep Sea Krill oil (Swisse, Collingwood Melbourne, Australia) were used as reference anti-inflammatory nutrients.
Hirsutanol A Attenuates
Lipopolysaccharide-Mediated Matrix
Expression and Cytokines Production and Improves Endotoxemia-Induced
- Materials and Methods 1. Materials
HA pretreatment significantly reduced the LPS-induced reduction in time spent in open space (p < 0.001). Similarly, in the current study, pretreatment with MEK inhibitor PD98059 significantly prevented LPS-induced ERK1/2 phosphorylation in THP-1 cells. Blocking STAT3 activity could reduce LPS-mediated inflammatory cell infiltration and damage in the lung [30].
Effect of HA on the number of CD68+inflammatory cells in the hippocampus after systemic LPS challenge.
Zoanthamine Alkaloids from the Zoantharian Zoanthus cf. pulchellus and Their Effects
Materials and Methods 1. General Experimental Procedures
Anti-inflammatory effects of a Mytilus coruscus α-D-glucan (MP-A) on activated macrophage cells via inhibition of the TLR4/NF-κB/MAPK pathway. For example, a glycogen polysaccharide extracted from Perna canaliculus has been shown to exert anti-inflammatory activities [7], and similarly, a heparin-like substance isolated from the marine mollusk Anomalocardia brasiliana has been shown to bind antithrombin. III (ATIII) and thus exert a strong anticoagulant effect [8]. In the present study, we isolated a high molecular weight α-D-Glucan, named MP-A, from hard-shelled mussels (Mytilus coruscus).
In the current study, LPS-induced THP-1 cells were used as an inflammatory model to investigate the effects of MP-A immunomodulation of THP-1 macrophages.
Materials and Methods 1. Reagents and Antibodies
When macrophages are activated by LPS, the TLR4 signaling pathway is initiated, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and activation of the transcription factor nuclear factor-kappa B (NF-κB) [21], and the induction of pro-inflammatory factors including NO, inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, and cyclooxygenase-2 (COX-2), etc. TNF-α and PGE2 were measured using a commercial ELISA kit according to the manufacturer's instructions. After three final washes (TBST buffer, 5 min), specific proteins were detected using an enhanced chemiluminescence kit (ECL, Millipore, Billerica, MA, USA) according to the manufacturer's instructions. β-actin was used as a loading control.
The affinity of MP-A for recombinant human TLR4, TLR2 and Dectin-1 was examined via SPR and performed using a BIAcore 3000 (GE Healthcare, Uppsala, Sweden) according to the manufacturer's instructions.
Results
Compared with control cells, LPS treatment significantly increased TNF-α and PGE2 production in THP-1 cells. In contrast, MP-A treatment inhibited TNF-α production by 60%. inhibition was achieved by treating cells with 200 μg/mL MP-A for 6 h. Figure 3. Effect of MP-A on TNF-α and PGE2 production and iNOS and COX-2 protein expression in LPS-stimulated THP-1 cells. ,D) MP-A inhibition of iNOS and COX-2 protein expression as assessed by Western blotting analysis in LPS-stimulated THP-1 cells.
MP-A prevented LPS-induced activation and the nuclear translocation of NF-κB in THP-1 cells NF-κB regulates many molecules of the different stages of the inflammatory response.
Discussion
Various inflammatory diseases upregulate pro-inflammatory cytokines (such as TNF-α and IL-1β) and inflammatory mediators (such as NO and PGE2) via the NF-κB and MAPK signaling pathways in macrophages [45,46] . Anti-inflammatory activity and mechanism of a lipid extract from hard-shelled mussels (mytilus coruscus) in chronic arthritis in rats. March. Anti-inflammatory activity of glycogen extracted from perna canaliculus (nz green-lipped mussel). Actions of agents.
Tumor necrosis factor (tnf) in inflammatory bowel disease: gene polymorphisms, animal models and potential for anti-tnf therapy.J.
Protective Effect of Pyrogallol-Phloroglucinol-6,6- Bieckol from Ecklonia cava on Monocyte-Associated
Results and Discussion
Previous studies on various biological properties of phlorotannins including antioxidant [17], anti-inflammation [18], anti-neurodegeneration [19], anti-cancer [20,21] and anti-cardiovascular disease [22] of E. Trans-migrating monocytes differentiated into macrophages of pro-inflammatory (M1-type macrophages) or anti-inflammatory (M2-type macrophages) types (Figure 2C-F). Furthermore, monocytes treated with PA-BSA contained elevated levels of inflammatory factors, including inducible nitric oxide synthase (iNOS), CD80, TNF-α, and interleukin-1β (IL-1β) ( Figure 2C,D ) and low levels of anti-inflammatories such as arginase-1 (Arg-1), CD206, transforming growth factor beta 1 (TGF-β) and interleukin-10 (IL-10) ( Figure 2E,F ) and the results were similar in Raw 264.7 (Figure S3B–E).
These proinflammatory cytokines induce the expression of adhesion molecules in ECs and recruit leukocytes, which are important components of the pathogenesis of vascular inflammation [26,27].
Materials and Methods 1. Materials
The lower chamber was filled with 500 μL of low-serum medium containing DK, PHB, PFFA, or PPB and 0.25 mM PA-BSA and incubated for 48 h in a 5% CO2 incubator. To analyze monocyte-associated EC viability, 5000 EC were seeded into wells of a 96-well culture plate (Thermo Fisher Scientific) and incubated for 24 h in a 5% CO2 humidified incubator at 37 °C. To analyze monocyte-associated VSMC proliferation, VSMCs were seeded in a 96-well culture plate (Thermo Fisher Scientific Inc.; Waltham, MA, USA) at 5000 per well and incubated for 24 h in 5%.
ECs were seeded at a density of 105 per well in a 100 mm culture dish (SPL Life Science; Pocheon, Korea) and incubated for 24 h in a 5% CO2 humidified incubator at 37 °C.
Conclusions
High glucose induces vascular smooth muscle cell proliferation by up-regulating the expression of the serine/threonine-protein kinase proto-oncogene Pim-1.Oncotarget. Kim, T.H.; Bae, J.S. Ecklonia extracts inhibit lipopolysaccharide-induced inflammatory responses in human endothelial cells. Food Chem. Akt Regulates Vascular Smooth Muscle Cell Survival through Inhibition of FoxO3a and GSK3.J.
Regulation of Vascular Smooth Muscle Cell Phenotype in Three-dimensional Coculture System by Jagged1-Selective Notch3 Signaling.Tissue Eng.
6-Bromoindole Derivatives from the Icelandic Marine Sponge Geodia barretti: Isolation and
Materials and Methods 1. General Produres
Compounds 2,3 and 4 showed anti-inflammatory properties by inhibiting DC secretion of IL-12p40 with different effects on IL-10, and the anti-inflammatory effect of compounds 2 and 3 was confirmed by inhibition of T cell IFN-γ secretion. matured and in co-culture. the presence of compounds. It is increasingly recognized that low-grade subclinical inflammation is an important pathogenic factor in many chronic diseases that until now have not been considered to be inflammatory in nature. Therefore, the discovery of two new 6-bromoindole derivatives with anti-inflammatory effects is important as they can be used in the development of treatments for diseases with inflammatory components.
Lipophilic fractions from the marine sponge Halichondria sitiens reduce secretion of pro-inflammatory cytokines from dendritic cells and reduce their ability to induce a Th1-type response by allogeneic CD4+ T cells. Pharm.
Prostaglandins in Marine Organisms: A Review
Structure, Biosynthesis, and Activity of Prostaglandins in Mammals
Prostaglandin E2 synthesis is a key event in the development of the three main signs of inflammation: swelling, redness, pain, and fever. PGD2 activity has been mainly associated with inflammatory conditions, being involved in important immunological functions. The emerging role of PGF2α in acute and chronic inflammation has opened new opportunities for the design of new anti-inflammatory drugs.
The PG A and J series contain anα,β-unsaturated carbonyl group within the cyclopentenone ring, which appears to contribute to the anti-inflammatory activity of these PGs [ 35 ].
Prostaglandins and Derivative Molecules in Marine Organisms
One of the first studies on marine invertebrates other than corals was a comparative analysis performed by Christ E. Indeed, PGE2 and PGF2α were identified in the ovary of the shrimpMarsupenaeus japonicusSpence Bate, 1888, where they participate in ovarian maturation (Table 2) [64] while in the crab Carcinus maenas Linnaeus, 1758 they are produced in blood cells after a stimulus induced with a calcium ionophore, in the presence of exogenous fatty acids (FA) [72]. Following this study, PGE2 was isolated and subsequently identified in the gastrointestinal tract and in the skin of the shark Triakis scyllia (Müller & Henle Figure 5b).
The presence of PGA, PGE and PGF series and their esters has also been documented in cyanobacteria of the taxaOscillatoria and Microcystidaceae [89].
Marine Cyclopentenone Prostaglandins
