Ket qua nghien c d u khoa hpc B V T V - S o 1/2018
kha ndng phdng trd bdnh rung Id cao d mdc d | khdc nhau d Thda Thidn Hue. Thuoc Score 250EC (hi$u lyc 64,4 - 69,9%) vd Tilt Super 300EC (hi$u lyc 55,4 - 69,4%) Id hidu lyc eao d ca hai vfing sinh thdi (vCing gd d l i d thj xd Huang Trd va vCing nui d huydn Nam Odng).
Sd dyng thulc Score 250EC, nong dd 0,1% vd Tilt Super 300EC, nong dd phun 0.05% d l phdng tri b#nh njng la eao su tgi Thda Thidn H u l .
d n nghidn cdu thdi gian va so lan phun thulc phCi hpp de tang hieu qua phdng trd b$nh n,ing Id cao su cOa cac loai thuoe.
Lm cim ort: Biy li kit qui cOa di tdi Khoa hoc vd COng ngh$ cip tinh duvyc ngin sich nhd nude tinh ThCra Thiin Hui diu tw, md s6 TTH.2015-KC.08.
TAI LIEU THAM K H A O
1 Campbell, C. L, L V. Madden, 1990. Temporal analysis of epidemics 1: description and comparison of disease progress cun/es, in Introduction to Plant Disease Epidemiology, John Wiley, New York, pp.
161-2021,
2. Ishii, H . Yano, K, Date, H., Fumta, Y., Sagehashi, Y., Yamaguchi, T., Sugiyama, T., Nishimura, K. Hasama. 2007. Molecular characterization and diagnosis of Qol resistance in cucumber and eggplant fungal pathogens.
Phytopathology, 97:1458-66.
3. Manju, M. J, 2006. Chemical control of Corynespora leaf fall disease. Corynespora leaf disease of Hevea brasiliensis Strategies for management (C. Kuruvilla Jacob, P Snnivas, 0. Bindu Roy). Rubber Research Institute of India, Kottayam, Kerala. India, p. 102-108.
4. Nguyen Thj Thu Thuy, Phan Th| Mpng Mo", Trin E)dng Hod, 2016 Nghiin cdu xdc i^nh tdc nhSn va budc diu danh gia hi^u qua mpt so thuoc hod hpc dli vdi benh njng Id cao su tai Thda Thidn Hul. T9p chl Bao v$ thiyc v$t. S6 4 (267): 21 - 27.
5. Phan Thdnh Dung, 2004. Ky thu$t b§o v$ thuc v$t cay cao su. Nha xult ban Ndng Nghiep.
6. Phan Thanh DQng, Phan Binh Thao, 2012. Quy trinh ky thuat cay cao su. T|p dodn cdng nghiep cao su Viet Nam, trang 138.
7. Srinivas P. and Idicula S.P , 2006. Fungicides in Corynespora leaf fall disease management. A Laboratory Manual for International Training on Strategies for Management of Corynespora Leaf Fall Disease of Hevea Brasiliensis (C.K. Jacob, P. Srinivas.
C. Bindu Roy). Rubber Research Institute of India, Kottayam, India, p. 40 - 49
8. Takeuehi. T., Kubo, C , H. Ishii. 2006. Sensitivity of Chiba Prefecture isolates of Corynespora cassiicola, the cause of Corynespora leaf spot on cucumber, to several fungicides. Annal report of the Kanto-Tosan Plant Protedion Society, 53: 55-60.
Phin bl^n: TS. Nguydn Thj Nhung
NGHIDN CLfU K H A NANG LPC C H ^ C O A NANO D C ) N G - SILICA CTC C H ^ s y PHAT TRI^N CUA N A M Pyricularia oryzea G A Y B E N H DAO ON HAI LUA Inhibition Effects of Copper - Silica Nanoparticles Against Rice Blast Disease
Caused by Pyricularia oryzae
Nguydn Thj Thu Thiliy^ Dd Thj Sen^ vd T r i n Thdi Hda^
Ngdy nhin bii: 03.12.2018 Ngdy chip nhin:15.12.2018
Rice blast disease, caused by Pyricularia oryzae. is the most serious biotic threat to rice (Oryza sativa L.) production worldwide. It causes severe yield losses in Vietnam especially in epidemic years. The fungus is highly variable so disease control is a challenge. In this study.
1. Khoa Ndng hpc, Trudng 9gi Hpc Ndng Ldm, Dgi hpc Hul
2. Khoa Hda, Trudng Dgi Hpc Khoa Hpc, Dgi hgc Hul 3. Ciy ban nhdn dan huypn Nui Thdnh, ti'nh Quang Nam
the effect of copper-silica nanoparticles (20-30 nm) against rice leaf blast fungus was evaluated under conditions both in vitro and in vivo. The application of five concentrations of copper-silica nanoparticles to the culture of P. oryzae showed significant inhibition of both
K i t qua nghien c d u khoa hpc B V T V - S o 1/2018
hyphal growth and number of colonies formed in a dose-dependent manner. Number of spores/ml decreased with in all treatment Copper-silica nanoparticles exhibited strong inhibition on the mycelium growth and spore production of P. oo^ae at 80 and 100 ppm. Under nethouse conditions, copper-silica nanoparticles were sprayed in concentrations 0, 20, 40, 60, 80 and 100 ppm on rice seedling leaves at 1 day after artificial inoculation with spore suspension). Damaged leaf area (%) indicated that the application of 80 and 100 ppm copper-silica nanoparticles was highly efficient before and after inoculation (9,37 and 7,67%), respectively compared to the untreated plants of 75,77%. The cun^ent study results offered a potential direction in the use of copper-silica nanoparticles for control blast nee diseases in crops.
Keywords: Copper-silica, nanoparticles, rice, Pyricularia oryzae 1. DAT V A N D E
Pyricularia oryzae thudc Idp n i m tiii (Ascomycefes), Id tdc nhdn gdy bdnh dgo dn vd Id mdt trong nhdng nguydn nhdn gdy thidt hgi kinh t l nhidm trpng nhit trdn todn t h i gidi (Ou, 1980). Khi cdy Ilia bi nhiem bpnh, tat cd cdc md Id ed the bi nam tan cdng. dde bipt Id khi bdnh gdy hgi trdn bdng cd t h i dan den m i t hodn todn ndng suit cCia ICia gao. Thidt hgi trung binh khoang 20 - 60%. d nhdng viing nhidm ndng, cd the mat hodn todn ndng suit (Zeigler vd cs, 1994). Thulc trd n i m Id bidn phdp chu yeu thudng dup^; s d dung d l kilm sodt bpnh dgo dn. tuy nhidn, s d dyng thulc didt n i m thudng gdy ra hidn tup'ng khdng thude, dong thdi dy lupng cOa thuoc cdnanh hudng d i n sdc khoe con ngudi vd gdy d nhidm mdi tordng (Minh Tuong Levdes, 2010).
Nghidn cdu vd dng dyng cdng nghd nano trong cdc ITnh vyc khdc nhau cija ddi song Id mdt trong nhdng hudng dang dup'c dgc bidt quan tdm hidn nay. Cdc san pham ngno thudng khdng ddc, phdn huy sinh hpc vd thdn thidn vdi mdi tardng. ChCing c6 t h i dng dyng tnang ndng nghldp nhd cdc hogt tinh smh hpe nhu: kfch thich s y nay m i m vd sinh trudng thyc vdt, Idm tdng hdm lupng chlorophyll, tdng k h i ndng hap thu dinh dudng cua cdy, idm giam stress....
(Belanger vd cs. 1995). Ngodi ra, ehung edn cd hogt tinh khdng nam, khdng khuin, khdng virus... vd dup'c dng dyng nhu Id thuoe bao vd thye - BVTV (Thurman vd cs. 1989; Young vd es, 2009). Nhieu nghidn cdu dd cho t h l y r i n g nano bgc, nano d i n g , nano titan dioxit duac s d dyng de bao quan ndng san. kieh thfch sinh trudng, phdt trien cua cdy trong, tdng kha ndng phdn hod choi, mam hoa, kfch thfch nay mam, ra r l . tdng khd ndng de khdng vdi m^t so lodi n i m gdy hgi cdy t r i n g (Shimosaka vd cs, 1993, Suchada vd cs, 2014). Trdn t h i gidi dd cd r i t nhilu cdng trinh nghidn cdu thdnh cdng cdc hpp chit nano d l phdng trd djch hgi trdn cdy trong vd dupe dng dung r|ng rdi. Nano bac dupe b i l t d i n vdi m|t kha ndng tidu di$t n i m vd vi khuan hgi
cdy trong r i t hidu qua, nd c6 t h i phdng trd dupe bdnh thoi hgt vd dao dn trdn cdy lOa do nim Bipolaris sokorinlana vd Pyricularia oryzae gdy ra (Gerasimenko vd cs, 2004), Nano kem cd kha ndng de che s y sinh trudng vd gdy hgi cua nim Penicilium expansum gdy bdnh m6c xanh trdn cdc ndng san p h i m sau thu hogch (Abdel Mawgoud vd es, 2013). Nano titan dioxit dup'c sd dyng d l phdng trd b$nh d i m Id trdn cdy lua ml vd cdy ngd, cung nhu hgn c h l bpnh bgc Id lua do vi khuin Xanthomonas oryzae gdy ra (Mujeebur vd Tanveer. 2014). Oligochitosan dd dupe sd dyng de phdng trd nam bdnh n i m gdy thli Altemaria alternata trdn tdo d Trung Quic (Yan vdcs, 2011). Tuy nhidn, d Vidt Nam ndi chung vd d Thda Thidn H u l ndi ridng vide dng dyng'cdc hgt nano trong cdng tdc bao vd thye vdt vdn cdn nhilu hgn che. Chinh vl vdy, muc tidu eua nghidn cdu ndy Id xdc djnh kha ndng dc che cua nano ding-siiica d i n s y phdt trien cua nam gdy bdnh dgo dn Pyricularia oryzae.
2. VAT LIEU VA PHU'aNG PHAP NGHIEN CCTU 2.1 Vat li#u nghien c d u
- Nano ding-silica (ning d | 300ppm;100ppm) cd pH=5, kich thudc 20 -30 nm do khoa Hda, Trudng dgi hpe Khoa hpc H u l eung d p .
- Nguin Pyricularia oryzae dup'c phdn Idp td cdy lua bj b^nh dgo dn tgi Thua Thidn Hud vd dup'c bao quan d phdng nghidn cdu b$nh cdy.
b | mdn bdo v$ thyc vdt, trudng dpi hpc Ndng Ldm H u l .
- Thdi gian nghidn cdu: t d thdng 12/2016 d i n thdng 9/2017, tai khoa Ndng hpc, trudng dgi hpc Ndng Ldm Hue.
2.2 Phtnyng phap nghien c d u
- Phuong phdp nghiin cOv dnh hu^ng cOa ning do nano ddng-sllica khic nhau din si/sinh truing cOa nim Pyricularia oryzae:
Mdi trudng PDA cd b l sung dung djch nano ding-silica vdi cdc n i n g 6^ 20. 40. 60, 80 vd 100 ppm. Cdc khoanh n i m 7 ngdy t u l i cd dudng
t qua nghidn c d u khoa hoc B V T V - S o 1/2018
kfnh 6 mm Qiegc cay vao trung tdm dTa peptri (0=9m) chda mdi trudng, nudi cay trong d i l u ki$n nhidt dp 28''C. Theo ddi dudng kfnh tan nam P. oryzea sau 3 - 5 ngdy nudi c i y (Elamawi vd El-Shafey, 2013). Kha ndng khdng n i m eua nano dupe xdc djnh nhu sau:
Hi^u lyc (%) = ((D-d)/D) X 100
Trong dd D (mm) id dudng kinh khuin igc n i m trdn mdi trudng PDA khdng bo sung nano (dli chdng),
d Id dudng kfnh khuin Igc nam trdn mdi tnjdng PDA cd b l sung nano d cdc nong dd khdc nhau.
- Phuryng phip nghiin cCfU anh hiiang cOa ndng dO nano ddng-sllica khic nhau din s y hinh thdnh bio tO nim Pyricularia oryzae.
Sau khi tdeh dan bdo td, bdo t d nam Pyricularia oryzae dup-e nudi e l y trdn dia peptri (0=9m) chda mdi trudng bdt gao-agar d d i l u kipn 25°C trong 10 ngay. Cho vao dTa bdo t d n i m 10ml nude c i t vd trCing, dOng que thCiy tinh gat lay h i t bao t d trdn dTa, hOt dung djch bdo t d vdo mdt ong nghidm thOy tinh, pha loang den nong dd 10^ bdo td/ml. Lay SOOpI dung dich bdo td nam cho vdo I n g eppendof chda dung dich ngno ding-silica d cdc n i n g d l khdc nhau tgo thdnh 1 hon hpp cd t h i tich 1ml. 0 cdc ong ndy trong tu djnh dn d nhidt dd 28°C trong 24 gid.
Sau dd hilt 25 pi hon hpp e l y Idn dTa peptri (0=9m) chda mdi trudng PDA vd u d 28°C, sau 10 ngdy c i y bdo t d , cho 1 ml nude c i t vd trCing vdo cdc dig thi nghidm, dCtng que thOy tinh trgng deu trdn b i mdt dTa thu dup'c dung djch bdo t d , pha loang dung djCh bdo t d vdi nydc cat vd triing vdi ty Id 1:10. Hut 50ul dung djeh bdo t d cho vdo buong d i m hong c l u , k i l m tra so lupng
bdo t d dudi kinh hien vi vd tfnh todn s i lupng bdo t d nam trong 1ml dung dich (Elamawi vd El- Shafey, 2013).
- Phuvng phip ddnh gid hi$u qui h$n chS b^nh d^o 6n h?l lOa cda rjano ddng -silica trong diiu ki^n nhd lutii
- Hgt gilng lua HT1 dup'c gieo frong cdc ehdu nhya cd kich thdc 30x30x30cm. Thi nghipm dupe b l trf hodn todn ngau nhidn vdi 5 cdng thdc ed ning dd dung dieh nano ding-silica khdc nhau (0, 20, 40^60, 80,100 ppm). moi cdng thdc nhdc lai 3 l l n , mdi lan nhdc Igi g I m 3 chdu, Thdi dilm phun nano dong-siliea: 1 ngdy tn/dc khi Idy nhilm nam.
- D i t thjt nhp dupe xu ly vdi vdi, trpn d i u vdi phdn chuing, phan NPK, sau dd cho vdo ede chdu nhya vdi lup'ng d i t bdng nhau, m l i chdu gieo 20 hat gilng.
- Ldy nhilm bang phuang phdp phun: cdy eon d 4 t u i n tudi (4-5 Id), phun 20ml dung djch nano d cdc nong dd khdc nhau Idn cdy Iila, de 1 ngdy sau dd phun 30ml dng dich chda 10^ bdo td/ml vd 1 % gelatin cho 1 chau liia. Bdt cdc chdu nhya trong nhd ludi, phun giu I m thudng xuydn trong khoang 16-24h, va gid nhidt dd khoang 24- 26°C Cdy lOa se dup'c k i l m tra sau 7 ngdy Idy nhiem, ddnh gid khd ndng dc che sy phdt triln cua b^nh dya vdo thang d i l m eCia IRRI. 1996
+ Bdnh dupe phdn cap nhu sau:
C l p 1 : < 1 % d i d n t l e h l d b | h g i d p 3 1 den 5 % dipn tieh Id bj hgi d p 5: > 5 den 25 % didn tich Id bj hgi d p 7: > 25 den 50 % didn tich Id bj hgi d p 9: > 50 % di$n tfch Id bj hgi.
Chl tiiu dinh gii-
s i Id bj bdnh Ty 10 Id bi bdnh (%)
s i Id d i l u tra
(ni+3n3+5n5+7n7+9n9)
Chl s6 bdnh trdn Id (%) = CSB (%) = 3 ( N x K )
Trong 66:- n^, ns,..., ng lin tu^Id sd Id bi binh 6cdc cip b^nh;
-Nii tdng sd Id diiu tra; K li cip b$nh cao nhdt quan sit dwgv - Phwang phdp x d ly sd Hiu
Cdc s6 li$u thu thdp dup'c phdn tich phuang sai mOt nhdn to va sai khde gida cdc nghidm thdc dup'c x d 1^ b i n g phan m i m IWicrosoft Office Excel 2007 vd phan m i m Stastitix 10.0.
3. K^T QUA NGHIEN Ct>U VA T H A O LUAN
3.1 Anh h u d n g cua n i n g dp nano ding-silica ddn s u sinh trudng cua n i m Pyricularia oryzae
Anh hudng cua ning dd nano ding-silica d i n dudng kinh tan nam trong thi nghidm dupe trinh bdy d bang 1 vd hinh 1. K i t qua cho &ily d cdc ning d | nano ding-silica khdc nhau. kha ndng sinh trudng vd phdt trien cda spi n i m Pyricularia oryzae
K i t qua nghiSn cdu khoa hpc B V T V - 8 6 1 / 2 0 1 8
Id khde nhau. Kich thudc tdn n i m giam dan theo d cdng thdc d l i chdng, dudng kfnh nhd n h i t d chieu tdng nong dd nano ding-siliea (Hinh 1). ning dd 100 ppm. Hidu lye dc che d i n sinh O thdi gian 3 ngdy sau c i y : dudng kfnh tan trudng ciJa n i m P. oryzae d n i n g d | 20, 40. 60, n i m d cdc cdng thdc thf nghidm giao ddng t d 0 80, 100 ppm lan luyt id 28,71%, 50.20%.
d i n 59,24 mm, trong 66 dudng kinh Idn n h i t id 63,96%, 82,16% vd 100%>
Bdng 1. Hi^u IIFC dc c h l cua nano ding-silica ddn d i n sinh trudng cda nkm P. oryzae nudi cdy trdn mdi tru'dng PDA ndm 2017
N6ng 66 nano dong-silica (ppm)
0 20 40 60 80 100
DLF^nq kinh tan nam (mm) 3 NSC
59,24' 42,23°
29,5' 21,35°
10,5°
0.00°
5 NSC 80,00"
62,47°
45.31°
38,5°°
26,67°
9.5°
Hi^u Ig-c 3 NSC
0,00 28,71 50,20 63,96 82.16 100.00
jec ch4 (%) 5 NSC
0.00 21.91 43.36 51.88 66.66 88,13 Ghi chO. NSC: Ngdy sau ciy;
'C^c gid trj trung binh dutmg kinh ^n nim theo cCt c6 cdc chO cdi in thudng khdc nhau sai Miic d'P^0,05 0 giai dogn 5 ngdy sau cay: dudng kfnh h$ sp'i
nam giao ddng t d 0 d i n 80,00 mm. cde cdng Hide cd sy khdc nhau d mCrc cd ^ nghTa thing kd. Trong dd cdng thirc doi chdng cd dudng kfnh tan n i m cao nhat vd cdng ^ d c nong dd 100 ppm cd dudng kfnh tan nam thap nhit. Nhu vdy c6 the thly, nano dong-silica ed kha ndng dc c^e sy phdt triln dudng kfnh tan nam P. oryzae, kha ndng ire e h l ndy tot nhat d mdi trudng PDA cd t)6 sung 100 ppm nano ding-silica (Hinh 1). Theo nghidn cdu
cua Elamviri vd El-shafey, 2013 sd dyng nano bgc cd kfch thudc khodng 20-30 nm vdi ning dp 200 ppm dc c h l hidu qud sy phdt triln cua nim R oryzea trong d i i u kidn In vitro. K i t qud khdo sdt hidu lyc khdng n i m C. Gloeosporioldes gdy b$nh thdn thu trdn cdy dt trong dieu kidn in vitro c^o ttily trong khoang n i n g d$ b l sung c h l p h i m td 20 d i n 80 ppm chitosan-ngno silica d i u cd tdc dyng irc c h l sy phdt triln cua tdn n i m tuang dng 15,6 d i n 67.2% (Phan Binh DGng vd cs, 2017).
Hinh 1. Anh hvdng cua nano dong-silica d i n sinh trudng P. oryzae sau 5 ngdy nudi cay tren moi t r u w i g PDA
Ket qua nghien c d u khoa hpc B V T V - S o 1/2018
3.2 Anh hydng cua nano ddng-silica d i n si^ hinh thdnh bdo t d ndm Pyricularia oryzae
K i t qud nghien cdu anh hudng nano d i n g - silica den sy hinh thdnh bdo t d P. oryzae dupe trinh bdy d bdng 2.
Bdng 2. Anh hvdng cua nano ddng-silica d i n s ^ hinh thdnh bdo t d ndm P. oryzae ndm 2017
Nong 30 nana d6ng- silica (ppm)
0 20 40 60 80 100
BSo t i j P. oryzae ( 1 0 ' b 4 o » / m l )
94,53"
36,48°
29,72°°
13.24°°
7.56°
0.0°
hinh 2. K i t qua thf nghidm cho t h l y d t i t c l cdc n i n g d$ x d ly nano dong-silica d i u ed khd ndng hgn ehe n h i l m bdnh v u y t trdi so vdi d l i chdng. Cdng thdc x d ly nano dlng-silica d n i n g dp 80 vd 100 ppm cd y nghia n h i t vdi ty Id nhidm b$nh chi Id 27,54% vd 25,42%, trong khi cdng thdc doi chdng tJ 1$ nhilm bdnh cd t h i Idn tdi 98,34%.
Bdng 3. Anh hudng cOa nano ddng-silica d i n ti 1$ bpnh {%) vd chi s6 b^nh (%) d^o dn trdn
cdy tOa tgi Thda Thidn Hue ndm 2017
Cdc gid tri trung binh sd lugng bio tCr nim theo cot c6 cdc chQ- cii in thut/ng khic nhau sai khdc a Pi 0,05
S i lup'ng bdo t d P. oryzae nudi trong mdi trudng PDA b l sung nano ding-silica d cdc ning d | khdc nhau thi khdc nhau vd giam d i n theo chieu tdng nong dd nano dong-silica. Mdt dd bdo td trong ede cdng thdc thi nghi$m giao ddng t d 0 den 94,53x 10* bdo td/ml, mdt dd bao t d d edng thdc doi chdng dgl cao nhat vd eao han t i t ca cdc cdng thdc edn lai d mdc y nghTa thong kd.
S i bdo t d P. oryzae dupe hinh thdnh khi nudi c i y trong mdi tni'dng PDA cd bo sung 20 vd 40 ppm nano dong-silica lan l u p l 36,48x10* bdo td/ml vd 29,72x10* bdo td/ml vd ed sai khdc cd y nghTa thong kd so vdi s6 bdo t d dupe hinh thdnh d cdc mdi trudng PDA dup'c b l sung 60, 80.100 ppm nano dlng-silica. So bdo t d P. oryzae hinh thdnh khi nudi trong mdi trudng PDA bo sung 80 ppm (7,54x10*-bdo td/ml) vd 100 ppm (0 bdo td/ml) khdng cd s y khdc bi$t cd y nghTa, nhung cd sy khdc bi§t cd J nghTa thing kd khi so vdi cdc nong d | cdn Igi.
Qua k i t qua nghidn cdu cd t h i k i t ludn r i n g nano dlng-silica cd kha ndng de c h l s y hinh thdnh bdo t d cQa n i m P. oryzae vd khd ndng de c h l t i l nhit d mdi trudng PDA b l sung 80 vd 100 ppm nano dlng-silica.
3.3 Khd nang hgn c h l b^nh dao do hgi lua ciJia nano ddng-silica trong d i l u ki^n nha ludi K i t q u i khao sdt hi$u lyc phdng bdnh dgo dn trdn cdy lua dup'c trinh bdy trdn bang 3 vd
Nong 30 (ppm) 0 (a/c) 20 40 60 80 100
Ty 1$ benh
(%)
98.34°
56.34°
54.65°
42.94°
27.54"
25.42"
ChTso benh
(%)
75.77°
29.23°
26.42'- 21.30°
9.37"
7.67"
Hinh 2. T^ 1$ b#nh vd chi s6 b^nh d^o on trdn cdy liia sau khi x d ly phun nano dlng-silica d
cdc n&ng d$ khdc nhau tgi ThCFa Thien H u l (ndm 2017)
Ngodi ra, k i t qud theo ddi chi s i b$nh cho thiv d ning dO 20 ppm dd hgn c h l han 50% chl so b$nh trdn cdc cdng thdc thi nghidm dupe xd ly vdi nano ding- silica, cht so b$nh thip nhit d cdng thirc bo sung nano dlng-silica d nong d | 80 vd 100 ppm Id 9.37% vd 7,67%. B i l u ndy dup'c gidi,thfch Id do nano dlng-silica d ning dd 80 vd 100 ppm tham gia hidu qua trang hidu dng phli hpp tgo cde phytoalexin dupe xem nhu Id m|t chit khdng sinh trdn thyc vdt (Luan vd cs. 2005). Mdt khdc. cdc hgt nano silica d l ddng dupe hap Uiu vd tich IQy trong cdc md bilu bi tdng cudng c l u fade cho cdc md ndy d l chIng Igi sy xdm nhdp cDa n i m b$nh. Cudi cCing, hgt nano Idm gia tdng hogt ding sdn xult cdc
Kit qug nghidn cdu khoa hpc B V T V - S 6 1 / 2 0 1 8
hpp chit phenolic vd cdc enzyme nhu chitinese nhdm chIng Igi sy xdm nhdp cDa m i m bdnh (Bore!
vd cs, 2014).
4. K £ T LUAN VA Dt NGHj 4.1 K i t luan
- Trong d i l u kidn in - vitro, ngno dlng-silica d nong dd xd ly d nong d$ 100 ppm cd hi$u lyc dc che sinh trudng cua nam P oryzae cao n h i t sau nudi cay 3 vd 5 ngdy l l n l u p l Id 100% vd 88.13%
- Tren mdi trudng nudi e l y nhan tao dupe x d ly vdi nano dlng-silica d n i n g dp 80 vd 100 ppm s i lup'ng bdo t d n i m dupe hinh thdnh thap n h i t 7,56 xlO* bdo td/ml vd 0 bdo td/ml so vdi d l i chdng Id 94.53 xlO* bdo td/ml
- Trong dieu kipn nhd ludi, x d ly nano d l n g - silica vdi nong dd 80 vd 100 ppm d thdi d i l m 1 ngay trudc khi Idy bdnh cd tdc dyng Idm giam ty Id b§nh tu 70.8% d i n 72,92% vd Idm gidm chl s i b$nh t d 66,4% d i n 68,1%.
4.2 Dd nghi
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