• Nghien CLFU - Ky thuat
structures were characterized by melting point, thin layer chromatography and IR, MS, 'H-NMR. " C - NMR spectra. The influences of the obtained compounds on the activity of cancer cells including liver cancer (Hep-G2) and lung cancer (Lu), were investigated. Notwithstanding, the experimental anticancer activity was not significant
T d i l i ^ u t h a m k h d o 1. Demlrayak S., Mohsen U. A., Ahmet C: "Synthesis and anticancer and anti-HIV testing of some pyrazino[1,2- a]benzimidazole derivatives', European Journal of Medidnal Oiemistry. (2002). 37, p. 255-260.
2 Singh J,. Graver P. and Pathak D. P.:
Synthesis, anticonvulsant activity and comparative QSAR study of some novel 1,2,5-trisubstituted benzimidazole derivatives, Ada Pharmaceutica
Sciencia, (2010), 52, p. 510-521.
3. Ramla M.M. Omar M. A. Khamryb A. M. and Dlwania H.: Syndesis and antitumor activtty of 1- subsliluted-2-methyl-5-nitrobenzJmldazoles, Boorganic &
Medicinal Chemistry, (2006). 14, p.7324-7332.
4. Hasan K., Dumnaz R., Orhan E. and Selami G.:
Synthesis, antibacterial and antifungal activities of electran-rich olefins denved benzimidazole compounds, // Farmaco. (2003), 58, p. 431-437.
5. Ramla M. M., Omar M. A., Tokudab H. and Diwania H • Synthesis and inhibitory activity of new benzimidazole derivatives against Burkltt's lymphoma promotion, Bioorganic & Medicinal Chemistry, (2007), 15. p.6489-6496.
6. Refaat H. M.: Synthesis and anticancer activity of some novel 2-sub5tituted benzimidazole derivatives, European Journal of Medicinal Chemistry, (2010),45, p. 2949-2956.
Nghien cii'u anh hirong cua cao hac phu, cao bach phu, cao kho phu tu toi chii'c nang
gan, than tren tho thuc nghiem
D a t v a n d e
Hac phu. bach phy vd cao khd phy ti> da du'Q'c nghien ci>u che bien d quy mo san xudt thi diem tip phy t i j thu tu- cdy 6 ddu trong 6- Sa Pa (Aconitum carmichaelii Debx.). CSc sdn pham thu du'Q'c cd chdt lu'p'ng on djnh, ed tdc dyng cu'&ng tim, du'Q'c sdr dyng trong edc thang thu6e hodc Idm nguyen li$u bdo c h ^ sdn phdm c6 phy ti>'''. Trong nhi>ng nghien ctPu tni'd'c ddy cho thay cao hac phy, cao bgch phy, cao kho phy ti> cd tdc dgng gay tdng bien dO co bdp tim, tang Iu-u lu-png mgch vdnh nhu-ng khong dnh hu'dng den tan s6 trdn tim tho c6 Igp'^^vd khong Idm thay d6i tinh trgng chung, trpng lu-Qng ca t h ^ vd chil-e nang tao mau trdn thd khi dCing lien tuc 28 ngdy*^. Tuy nhien, ed cau hdi du-QC dgt ra II0U khi dCing edc cao nay cd dnh hu'd'ng den chi>c nang ciJa edc c o quan quan trQng khde nhu' gan vd thgn hay khong. Vi vgy, dk cd them
34
Bui Hong Cirdng', Nguyin Trpng Thong' Tnrang Dgi hgc Dufrc Hd NQI
^Tnrang Dgi hgc YHd Ngi
CO sd' khoa hpc trd Id-i cho cdu hdi trdn, d^ tdi Nghiin ci>u anh huvng cua cao hie phii, cao b^ch phy, cao khd phy td' ti/i chvc nang gan, th$n tr&n tho d u v c ti^n hdnh.
D o i l u ' p ' n g v a p h u ' O ' n g p h a p n g h i e n CIFU
M i u nghidn CIPU Hie phu, bgc/i p/jy
NguySn li$u du'Q'c bdo c h ^ dgng cao khd:
Ndu 3 l^n vd'i nu'dc, thd'i gian soi moi lan 1 gid.
G Q P cdc djch chl^t cd d i n dgng cao Idng 1:1.
£)4 l^ng logi tgp, ggn djch trong. c6 cdch thu^
d6n d$c, sdy khd, xay bdt, 1 g cao kho tu-cng Crng 5g du'Q'c ll§u. Khi thi> hoa tan Igi bdng nu'dc den the chdt thich hp'p.
Cao khd pht^ tu-
DU'Q'C bdo c h i 6- quy md sdn xudt thf d i i m tCr phy tu- phiin'^', 1g cao tu-ang u-ng 5 g du'Q'c ii§u.
TAP CHI DirgtC H Q C - 05/2012 ( S 6 433 N A M 52) i
• Nghien CLFU - Ky thuat
Khi thu" hod tan cao khd phy t i i vdo nude vu-a du den the chat thich h(?p.
Dong vgt thi nghidm
Thd Chung Newzealand White, long trang, trpng lu'p'ng 1,8 - 2,5 kg do Trung tdm Chan nudi - Vi$n Kiem nghlgm cung cap.
Tho du'Q'c nuoi trong i ^ i u kl#n day du thd'c an va nu'dc uong tgi Phdng thi nghidm Bd mon DU'Q'C 1^, Tru'dng Bgi hpc Y Hd Ndi tCr 3 - 7 ngdy tru'de khi nghiSn c d u va trong sudt thdi gian nghidn c d u .
Hod chat va di^ng cy thf nghidm Kit dinh lu-o-ng cdc enzym vd chdt ehuyin hoa trong mdu: ALT (alanin aminotransferase), AST (aspartat aminotransferase), bilirubin todn phan, albumin, cholesterol va creatlnin eua hang Hospitex Diagnostics (Italy) va hang DIALAB GmbH (Ao), djnh lu-Q-ng tren mdy Screen master cua hdng Hospitex Diagnostics (Italy); cdc hod chdt Idm tidu ban md b^nh hpc dgt tieu chuan phong thf nghiem do Phdng Giao tdi Tru'dng Dgi hpc Y Hd NQI cung c i p .
Phirong phap nghien CLFU
Thd du'Q'c chia Idm 7 Id, mdi Id 10 eon, m5i con nhot rieng mot chuong.
- Lo chdng: u i n g nu'dc cat 2 ml/kg/ngdy.
- Lo t h d 1, 2, 3: uong cao kho hac phy, eao khd bgch phy, cao khd phy t d tu'ong dng, l i i u 60 mg/kg/ngdy (liiu cd tdc dyng tu-ang du-ang trdn ngu'di, tinh theo h^ s6 3).
- Lo thd 4, 5. 6: u i n g eao kho hac phy, cao khd bgch phy, cao khd phy t d tu-cng dng, l i i u 300 mg/kg/ngdy ( g i p 5 l i n Id trj 1, 2, 3).
Thd du'Q'c u i n g nu'dc hogc t h u i c trong 4 tuan l i i n , m6i ngdy mdt ldn vdo b u i i sdng, vdi the tfch 2 ml/kg. Danh gid chdc ndng gan thdng qua dinh tu'Q'ng mQt so enzym vd c h i t c h u y i n hod trong mdu (ALT. AST. bilirubin todn p h i n , albumin vd cholesterol); ddnh gia chdc nang thdn thdng qua djnh lu-gng creatlnin h u y i t thanh d cdc thdi d i i m tru'de u i n g thuoc, sau 2 tuan va sau 4 tuan uong t h u i c '^^l Sau 4 tuan uong t h u i c , thd du'Q'c mo de quan sdt dai t h i todn bo cac ea quan vd kiem tra ngau nhidn c i u true vi the gan, thdn cda 30% so thd d moi Id. Cdc xdt nghiem vi t h i du'Q'c thyc hign tgi BQ mdn Giai phau benh - Tru'dng Dai hpc Y Ha Npi.
X d ly s 6 li#u bang phu'ong phap thong ke y sinh hoc, k i t qua thi nghlgm duQC b i i u thj bang trj so trung binh cpng/trd sai s6 chuan ( J i SE).
K e t q u a n g h i e n CLPU
Anh hu'dng cua cao hac phu, cao bach phu, cao phu t d tdi ctidc nang gan tho K i t qua nghien c d u du-QC t h i hidn qua cac bang 1-5.
Bdng 1 : Anh hudng cOa cao hie phy, cao b^ch phi^, cao kho phy f d din hoat dd AST trong mau tho Mau nghiin cuv Thdi gian Ho^t do AST (Ul^) p (t- test Student
Cao hSc phg
Cao bach phu To T,4 p (tapdc - sau)
Ta p (tru-d-c - sau)
To T u p (tru'de - sau)
T„
p (tru'd'c - sau) To T u p (tnr&c - sau)
T28
Lo chirng 38,12 ±2.42 44,12 ±3,50
>0,05 43,41 ± 6,56
>0,05 L6 chirng 47,26 ±6,82 31,76 ±3,81
>0,06 32,76 ±1,28
>0,06 L6 Chiang 38,12 ±2,42 44,12 ±3,50
>0,05 43,41 ± 6,56
L6 thir 1 36,49 ± 3,29 47,90 ±4,16
>0,05 46,26 ± 6,65
>0,05 Ldthir2 42,61 ±5,46 39,46 ± 5,07
>0,05 35,71 ± 2,00
>0,05 L6thti-3 37,97 ±1,95 46,94 ±5,16
>0,05 43,86 ± 6,22
LSthv4 40,37 ± 2,95 45,76 ± 3,67
>0,05 36,92 ±4,74
>0,05 LothO'S 42,20 ± 3,78 41,71 ±8,24
>0,05 43,21 ± 4,80
>0,05 L6thir6 42,04 ± 2,67 41,10±6,45
>0,05 39,16 ±4,11
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>o,a5
>0,05 p (tru'de - sau)
Ghi chu: To: Trucrc udng thudc, Tu: Sau 2 tuin uong thudc, TIB'. Sau 4 tuin uong thudc
TAP CHi DirOC H p c - 05/2012 (SO 433 NAM 52)
• Ngiiien ciru - Ky thiuat
Bang 2: Anh huifng cOa cao hie phu, cao b^ch phu, cao l<h6 phu tCr din ho^t dO ALT trong miu thd
Mau nghl6n ciru Thirl gian Ho^t aj ALT (Ul/I) p (t- lest Student)Cao hSc phu
Cao bach phg
Cao kh6 phy tu' TO T14 p {\nrirc - sau)
T2e p (tru'&c - sau)
TO T14 p (thuo'c - sau)
T28 p (truO-c - sau)
TO T14 p (tnyirc - sau)
T28
L6 chimg 57,50 ± 2,07 58,8111,59
>0,06 62,79 t 5,00
>0,05 L6 chirng 64,69 ± 5,04 55,84 ± 2,28
>0,06 56,78 ±1,22
>0,05 L& chimg 57,50 ± 2,07 58.81± 1,59
>0,05 62,79 ± 5,00
L6 thir 1 57,88 ± 2,75 61,31 ±2,91
>0,05 63,93 ± 5,75
>0,05 L6thir2 59,39 ± 3,39 60,12 ±2,75
>0,05 57,67 ±1,38
>0,05 L6thir3 59,92 ±1,90 63,47 ± 3,65
>0,05 65,68 ±6,17
L6 thir 4 60,78 ± 3,07 66,26 ± 3,03
>0,05 56,40 ± 3,57
>0,05 L6 thir 5 59,84 ±2,10 60,93 ±4,55
>0,05 62,33 ± 2,74
>0,05 Ldthire 59,06 ±1,85 62,79 ± 4,41
>0,05 59,44 ± 2.75
>0,05
>0,05
>0,05
>0,05
>0.05
>0,05
>0.05
>0.06
>0.05 p(tru'6'C-sau)
Bdng 3: Anh hu^ng cua cao hie phy. cao b^ch phy, cao kho phy tu" din ndng d0 bilirubin toan
phin trong m^u thdMiu nghien ciru Thoi gian Bilirubin toin phan (mmol/l) p (t- test Student
Cao hac phg
Cao b^ch phg
Cao kho phy ti>
TO T14 p (tru'de - sau)
T28 p (tru'de - sau)
TO T14 p (tru'de - sau)
T28 p (trudc - sau)
TO T14 p (tru'de - sau)
T28
L6 chirng 12,39 ±0.10 12,27 ±0.16
>0,05 12,39 ±0,12
>0,05 L6 chimg 12,43 ±0,10 12,34 ±0,14
>0,05 12,30 ±0,14
>0,05 t d chimg 12,39 ±0,10 12,27 ±0,16
>0,05 12,39 ±0,12
L6 thir 1 12,43 ±0,11 12,46 ±0,16
>0,05 12,28 ±0,14
>0,05 L6 thir 2 12,41 ±0,13 12,19 ±0.18
>0.05 12.37 ±0.13
>0.05 L6thi>3 12,43 ±0.18 12.20 ±0.19
>0,05 12,32 ±0,12
i.au>i>4 12,24 ±0,12 12,41 ± 0.08
>0.05 12.18±0.16
>0.05 l.dtlll>5 12,33 ±0,13 12,22 ±0,12
>0,05 12,34 ±0,10
>0,05 L6thU'e 12,25 ±0,10 12,25±0,13
>0.05 12.34 ±0,13
>0,05
>0.05
>0.05
>0.05
>0.05
>0.05
>0.05
> 0.05
>0.05 p (tru'de - sau)
T^P CHI DlTQC HQC - 05/2012 (SO 433 NAM 52)
• Nghien ciJu - Ky tiiuat
Bang 4: Anh miu tho
Mau nghtSn ctj^
Cao hac phu
Cao bach phy
Cao khfi phg tCp
hu&ng cda cao
Thdi gian
TO T14 p (tru'de - sau)
T28 p (tru'de - sau)
TO T14 p (tru'de - sau)
T28 p (tru'de - sau)
TO T14 p (trude - sau)
T28
hie phu, cao b^ch phu, cao khd
L6 chu'ng 3,77 ± 0,23 3,70 ±0,15
>0,05 3,41 ±0,12
>0.05 L6 chirng 4,41 ±0,36 5,10 ±0,19
>0,05 3,96 ± 0,25
>0,05 Ld chirng 3,77 ± 0,23 3,70 ±0,15
>0,05 3,41 ±0,12
Albumin (g/dl) L6 thir 1 3,38 ±0,19 3,89 ± 0,27
>0,05 3,58 ± 0,20
>0,05 L6 thir 2 4,30 ±0,18 4,98 ± 0,32
>0,05 3,72 ± 0,27
>0,05 t a thir 3 3,29 ± 0,08 3,79 ± 0,34
>0,05 3,83 ± 0,22
phu tir din ndng d0
L6thir4 3,40 ±0,10 4,01 ± 0,24
>0,06 3,82 ± 0,22
>0,05 LothirS 4,14 ±0.14 4,76 ± 0,44
>0,05 3,75 ± 0,08
>0,05 Lo thir 6 3,56 ±0,13 3,83 ± 0,27
>0,05 3,84 ± 0,24
p(t-
atbumin trong
test Student)
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
p (tryac - sau) >0,05
Bang 5: Anh huong eua cao hie phy, cao bach phy, cao kho phy (d din nong do cholesterol trong mau tho
Mau nghien cuv Thai gian Cholesterol (mmol/l) p(t- test Student)
Cao hae phy
Cao bach phu
Cao kho phg tO To T u p (tru'de - sau)
T i , p (tru'de - sau)
To T u p (tru'de - sau)
T2B p (tryde - sau)
To T u p (tru'de - sau)
T28
L6 chOng 2,37 ± 0,09 2,48 ± 0.08
>0.05 2.50 ± 0.08
>0.05 L6 chirng 2,41 ± 0,07 2,60 ±0,17
>0,05 2,35 ±0,17
>0,05 Lo chimg 2,37 ± 0,09 2,48 ± 0,08
>0,05 2,50 ± 0,08
Ld thir 1 2,49 ± 0,09 2,64 ± 0,09
>0,05 2,62 ± 0,09
>0,05 L6thi'2 2,30 ± 0,07 2,56 ±0,14
>0,05 2,39 ± 0,09
>0,05 LdthtS 2.38 ±0.11 2.60 ±0.11
>0,05 2,46 ± 0,08
Lo thi' 4 2,57 ± 0,07 2,56 ±0,12
>0,05 2,55 ±0,10
>0,05 L6thir5 2,36 ± 0,09 2,30 ±0,13
>0,06 2,27 ± 0,09
>0,05 LothiH 2,63 ±0,13 2,69 ±0,12
>0,05 2,67 ±0,10
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05
>0,05 p (tru'6'c - sau)
J TAP CHJ D i r g e HOC - 05/2012 (S6 433 NAM 52)
0 Nghien CLFU - Ky thuat
Nh$n x6t: Sau 2 tudn vd 4 tudn u i n g cao hde phy, cao bgch phy. cao khd phy t d , t i t ed cdc xdt nghidm ddnh gid chdc ndng gan (ALT, AST, bilirubin todn p h i n , albumin, cholesterol) d cd Id t h d 1, 2, 3 ( u i n g l i i u 60 mg/kg/ngdy) vd Id t h d 4, 5, 6 ( u i n g l i i u 300 mg/kg/ngdy) d i u khdng cd si^ khdc bi$t cd y nghTa so vdi Id chdng vd so
sdnh glda hai thdi d i i m tru'de vd sau khi u i n g t h u i c (p > 0,05).
Anh h u ^ n g cua cao h i c ph^, cao bgch phg, cao phg t d tdi chi>c nang th^n thd
Anh hu'dng eiia eao hdc phy, cao bgch phy, cao phy t d tdi ehde ndng thdn thd dup'c ghi trong bdng 6.
Bdng 6: Anh huimg cOa cao hie phy. eao b^eh phy, cao khd phy tO' din ndng dd creatlnin trong mdu thd
Miu nghl6n cdu Thdi gian Creatlnin (mg/dl) p (t- test Student) L6 chimg
Cao hie phy
p (trudc - sau)
p (tru&c-sau)
L6 chCmg
p (tru'de - sau)
p (tru'de-sau)
L6 chOng
p (tru'de-sau) T28
p (tru'de-sau)
>0,05
NhSn xdf; Sau 2 t u i n vd 4 t u i n uong cao hdc phy, cao bgch phy, cao khd phy t d , d ed Id t h d 1, 2, 3 ( u i n g lieu 60 mg/kg/ngdy) vd Id thd 4, 5, 6 (uong l i i u 300 mg/kg/ngdy), n i n g dO creatlnin trong mdu thd khdng cd SI.T thay doi khdc bi@t so vdi Id chdng vd so sdnh gida hai thdi d i i m tru'de vd sau khi u i n g t h u i c (p > 0.05).
Anh hu'dng cua cao hSc phg, cao bgch phu, cao phg t d ve md b$nh hpc d i n gan, than thd
Dai thi
Trdn t i t ca cac thd t h y c nghi#m (cd 16 chdng va 6 Id thd). khong quan sdt t h i y cd thay d i i benh ly ndo v i mgt dai t h i cOa cdc co' quan tim, phoi, gan, Idch, tyy, thdn vd hO thong tidu hod cua thd.
VI thi Hlnh th&i vi thi gan
Cao hdc phy d cd hai l i i u t h u i c da thd trdn thd d i u cd b i i u hi^n t i t , lam tdng d y tni' glycogen trong t i bdo, nhu'ng d lieu cao g i p 5 l i n l i i u thu'dng dung tren ngu'di (300 mg/kg/ngdy) da gdy t i n thu'ong thodi hda t i bao gan d 1/3 m i u b^nh p h i m .
Cao bgch phy dCing tren thd lieu 60 mg/kg/ngdy (lieu cd tdc d y n g tu'ong du^ang trdn ngu'di) khdng gdy t i n thu-cng t i bao gan n h y n g d l i i u cao g i p 5 lan l i i u thu'dng dung trdn ngu'di (300 mg/kg/ngdy) gay t i n thu'ong thodi hda h i e nhg t i bdo gan d 2/3 m i u bdnh pham.
T ^ CHf DirOC HQC - 05/2012 (S6 433 N A M 52) I
• Nghien CJLJPU - Ky thuat
Cao phu t d dCing tren thd lieu 60 mg/kg/ngdy (lieu ed tac dyng tu-cng du'O'ng tren ngu'di) khdng gay ton thu-o-ng te bdo gan nhu-ng d l i i u cao gap 5 lan lieu thu'dng dCing trdn ngu'di (300 mg/kg/ngay) gdy t i n thu'ong thodi hda gan d 2/3 m i u bdnh pham.
Hinh thai vi thi th$n
Cd hai lieu cao hac phy. cao bgch phy, cao phu t d deu khdng anh hu'dng ro rgt d i n c i u tnic vi t h i thgn thd.
Ban luan
Theo y hpc c i truyen, phy t d che cd tfnh dgi nhigt. cd dQC, l i i u eao cd tdc dyng h i i du'O'ng cdu nghjch, trj chdng thodt du-ong, vong du'O'ng {tn,jy tim mgch c i p ) , dung l i i u t h i p , keo dai cd tdc dyng bo du'ong, bo hda ( b i tdm. thgn du'O'ng). Day Id vj t h u i c cd hogt tinh mgnh, Id mOt trong b i n dau vj (sdm, nhung. que, phu).
nhu'ng ed the gay ngp dpc n i u c h i bien va s d dyng khdng dung. K i t qua nghidn cdu cho thay ca 3 san pham cao hac phy, cao bgch phu, eao phy t d dung d lieu tu'ong du'O'ng lieu s d dung' thdng thu'dng tren ngu'di khdng gay anh hu'dng den chdc nang gan. than. Tuy nhidn, d l i i u gap 5 lan heu ttiu-dng dung tren ngu'di. trong 4 t u i n deu gay ton thu'cng thodi hda nudc, thodi hoa hgt, thodi hda hdc nhg te bdo gan. Mae du ed t i n t h u c n g tren vi t h i gan d mpt so thd u i n g liiu gap 5 l i n lieu d y kien trdn ngu'di nhu'ng cac xet nghigm sinh hda chu'a thay s y thay doi ro rgt ed the do thuoc t h d gdy ra ton thu-ang d mdc dp nhe va khu tni nen cdc vung khdng t i n thu'ong cd hogt dpng bCi t r d . K i t qua ndy eung cdnh bdo can h i t s d c than trong khi s d dyng phu t d d lieu cao trong thdi gian dai.
Ket luan
Mau nghien c d u cao hac phy, cao bgch phu, cao phy t d cho thd u i n g lieu 60 mg/kg/ngdy (lieu tu'Q'ng d u o n g vdi l i i u dung d ngu'di) trong 4 tuan liin, t i t ca cac chf so theo doi v i chdc nang gan, chdc ndng than va md benh hQC gan. thdn d i u nam trong gidi hgn binh thu'dng, khong cd s y khde biet rd ret so vdi Id chdng. Khi eho thd u i n g cao hdc phu, cao bach phy, cao phy t d l i i u eao gap 5 l i n lieu thu'dng dOng tren ngu'di (300
mg/kg/ngdy) trong 4 tuan l i i n , tat ed cdc ehi so theo ddi ve ehdc ndng gan. chdc nang thdn vd md bgnh hpc thdn d i u nam trong gidi hgn binh thu'dng, khdng ed s y khac bigt rd rgt so vdi 16 chdng, nhu-ng trdn md bgnh hpc gan cd b i i u hign t i n thuong thodi hda nude, thoai hda hgt, thodi hda h i e nhe t i bdo gan.
Ldi c d m o n : Cong trinh nay thupc Oe tai h i try kinh phi Nhd n u d c theo Nghj djnh 119/1999/NO-CP Ciia Chinh phii. Chiing toi xin chan thdnh edm on Cdng ty CP Traphaco vd Bd Khoa hpc vd Cong nghg da tai try kinh phi, giiip chiing t6i hodn thdnh cong trlnh ndy.
S u m m a r y
Experimental effects of the extracts from Heishunpian. Baifupian and Aeoniti on the liver and kidney functions were studied in rabbits. Each separate individual extract, at the daily oral dose of 60 and 300mg/kg body weight in a course of 4 weeks on end, no changes were observed in the plasma levels of AST. ALT, bilirubin, albumin, cholesterol, and creatlnin in rabbits. After 28 consecutive days' administration of the extracts, no morphologic damages were seen on macroscopic organs and microscopic kidney images; but, on microscopic liver images, the liver cytolysis at various levels was seen in the group of rabbits given 300mg/kg body weight per day.
Key words: Heishunpian, Baifupian, Extractum Aeoniti. Aconitum carmichaelii Debx.
Tai lieu tham khao
1. Bui Hing Cudng, Phung Hoa Binh: Che bien hac phu. bgch phg va bSo c h i cao kho phu t d d quy mfl pilot, T^p chi Duac hgc (2010), (8) tr. 32-37.2. BCii Hing Cudng, Nguyen Trpng Thong.
Nghien cO-u doc tinh c i p va anh hu'dng cua cao hac phy, cao bach phg, cao khd phg td tdi tinh trang chung vd chdc nang tgo m^u tren dpng vdt thyc nghi?m, T$p chi Dugc hgc, (2011), (3) tr. 49-54.
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