T»P CHI Y DLfgC HQC CAN THff - s 6 11-12/2018
9. Ottani F, Galvani M, Nicolini FA, Ferrini D, Pozzati A, Di Pasquale G, et al. Elevated cardiac ttoponin levels predict the risk of adverse outcome in patients with acute coronary syndromes. Am Heart J. 2000;140: 917-927.
10. Holzmann MJ, Sartipy U. Relation between preoperative renal dysfunction and cardiovascular events (sttoke, myocardial infarction, or heart failure or death) within three months of isolated coronary arteiy bypass grafting. Am J Cardiol. 2013;112: 1342-1346.
11. Berton G, Cordiano R, Cucchini F, Cavuto F, Pellegrinet M, Palatini P. Atrial fibrillation during acute myocardial infarction: association with all-cause mortality and sudden death after 7-year of follow-up. Int J Clin Pract. 2009;63: 712-721.
12. Piccini JP, Hranitzky PM, Kilam R, Rouleau JL, Whhe HD, Aylward PE, et al. Relation of mortality to failure to prescribe beta blockers acutely in patients with sustained venfricuiar tachycardia and ventticular fibrillation following acute myocardial infarction (from the VALsartan In Acute myocardial iNfarcTion trial [VALIANT] Registry). Am J Cardiol. 2008;102: 1427-1432.
(Ngdynhgn bai: 14/11/2017-Ngay duyet dang: 08/01/2018)
PHAN LAP VA N U O I CAY i t BAO G O C T R U N G M 6 TlT M O M 0 C H U O T N H A T T R A N G {SWISS)
Nguyin Thanh Tha/*, Trdn Ngpc V&\ Chi Thi Cdm H^
1. Khoa Huyet hoc - Binh viin Trung Ifang Hui 2. Bgi hoc Khoa hoc Hui
*Email: [email protected] TOM TAT
Bgt van de: Ti bdo gdc la te bdo chuyin biet, cd khd ndng tu ddi mdi thong qua phdn chia ti bao vd biet hod da ddng.Tibdogdc trung md tir m& cd tinh chdt ire chi - diiu hod miin djch.
tiet cdc cytokine va ede thu the miin djch dieu chinh vi mdi tnrdng ti-ong ghep. Vdi khd nang diiu biin mien dfch vd tiit cdc phdn tu chdng viem nin ti bdo goc trung md Id cdng cy hiiu qua trong diiu tii cdc binh mdn tinh. Muc tiiu nghiin cuu: nghiin cdu phdn lap sd luang ti bdo dan nhdn tir md md qua thdm dd ndng do collagenase vd thdi pan u collagenase. Boi tirgng va phirffng phdp nghiin cuu: Su dung chugt nhdt trdng gidng Swiss. Trong nghien cuu ndy, chung tdi khdo sdt ndng dg vd thai gjan u ciia collagenase cho viec phdn lap te bao gdc trung mo ma vd nudi cdy ti bdo gdc trung mo tu md. Ket qua: d mdi 1 mg md/mL collagenase it 50 phut thu dugc cdc te bdo dan nhdn cao nhdt: 4,77x105 ± 0,31x10^ ti bdo/g vd hiiu qud phdt tiiin ti bdo dgt trin 69,33%. Kit lugn: diing enzyme collagenase, it 50phdt, chimg toi thu nhgn dugc ti hao gdc trung md nhiiu nhdt
Tir khod: ti bdo goc trung md tu ma, enzyme collagenase, u, nudi cdy, biet hod.
ABSTRACT
ISOLATION A N D C U L T U R E OF A D I P O S E - D E R I V E D MESENCHYMAL STEM CELLS F R O M MICE(Swiss) A D I P O S E TISSUE
Nguyen Thanh Thuy, Tran Ngoc Vu\ Che Thi Cam H(^
I. Hue University, College of Sciences.
2. Hue Centi-al Hopital
TAP CHf Y DUQt HQC CXN THg - s 6 11-12/2018
Background: Stem cells are cells specialized cell, capable of renewing themselves through cell division and can differentiate into multi-lineage cells. Adipose-derived mesenchymal stem cells (ASCs) display immunosuppressive properties, immunomodulatory features, secrete cytokines and immune-receptors which regulate the microenvironment in the host tissue.
Multilineage potential, immunomodulation and secretion of anti-inflammatory molecules makes MSCs an effective tool in the treatment of chronic diseases. Objectives: The aim of this study was to investigate the concentration and the incubation time enzyme collagenase. Materials and Methods: research animal model mouse Swiss. In the present study, we have explored the concentration and incubation time enzyme collagenase for isolate ASCs and culture MSCs derived from adipose tissue ASCs. Results: 1 mgfats / ml collagenase. incubate for 50 minutes, we have
the highest monolayers: (4.77± 0.31)xl6^ cells / g and 69.33% cell ^owth efficiency.
Conclusions: Using enzyme collagenase. incubated for 50 minutes, we received the hightest mesenchymal stem cells.
Keywords: Adipose-derived mesenchymal stem cells, enzyme collagenase, incubation, in vitro, differentiate
L D^T VAN BE
Md ma cd khd nang tdng hgp va tiet mdt sd enzyme, ySu td tang trudng, cytokine, hormone tham gia diSu hda phan iing viSm. TS bao goc trung md tir md ma md ra tiSm nang ttong diSu trj do tinh chat lie che miSn dich va tinh miSn dich thdp. thich hgp cho cdc img dung lam sdng [7].
Te bdo gdc trung md la quan thS tl bao tdn tai rdt it d cac d cua chung tai cdc md vd cd khd nang biet hoa da dang ttong mdi trudng nudi cdy thich hgp [3], [6]. Thu nhan tS bao gdc trung md tu rat nhiSu ngudn khdc nhau [15]. Md mo cd tinh mSm deo cao vd kha nang biet hod thanh te bao mo, te bao xuong, te bao co, tl bao gan, tS bao thdn kinh va cdc tS bdo ndi md va biSu md [3]. Hieu qua te bao gdc tnmg md tir mo da dugc chiing minh ttong y hgc tai tao vdi sd lugng la 10^ tS bao/kg the ttgng [9], [10].
Bai bao ndy gioi thi$u mpt sd ket qua nghiSn cim vS phan lap, nudi cay va xdc dinh ddc tinh tS bao gdc trung md tir md md chugt nhat trang (Swiss) bang phuang phap enzyme.
II. PHU'ONG PHAP NGHIEN CUtJ
Doi tirgrng nghiSn cihi: Md mo trdng dudi da dugc thu tir chugt nhat ttang (Swiss), 8-10 tuan tudi, nang 55,7 ± 0,57 g, dSu la con due khde manh.
Hod chdt - Duog cij: Tiypsm-EDTA 0,25% (Sigma-Aldrich), huyit tiianh thai bd (FBS) (Sigma-Aldrich), penicillin/stteptomycm:l%, tiypan blue 0,4%, PBS, DMEM/F12 (Gibco, Life Technologies), collagenase B (Roche), etiianol 96% (W.S.P), formaldehyde 36% (Eden-labo), eosm 2% (Theralab), Giemsa May-Grunwald (Cypress Diagnostics), Oil red-O 0,5% , mang Ipc 70^m (Biologix).
Phirong phap nghien ciru:
Thu mo ma vd xu l;y sa bg: TiSn hanh thao tac ttong budng cay vd tning, giSt chupt bdng keo gian ddt sdng cd. Giai phau bdc Id xoang bung chuOt, tdch va tiiu md md cho vao ethanol 70%, nia bdng dung dich PBS khdng sinh/iic chS nguySn phan: 1%
(antibiotic/antimitotic:l%), penicillin/streptomycin: 1%.
Phucmg phdp thuy phdn md ma bdng enzyme collagenase vaphdn lap ti bdo dan nhdn: Cdt nhd Ig md ma bang dao tieu phau, cho vao dng falcon v6 trimg, cho them 1 ml DMEM/coIlagenase (mg/mL) va vortex. Sau dd ii 15 phiit nhiet d$ phdng va chuySn vao ii
TAP CHi Y DU'gfC HQC CAN THg - SO 11-12/2018
50 phiit ttong tii dm d nhiet dp 37°C, khuay nhe liSn tuc sau 10 phiit. Lpc bdng mang lpc 70pm va rita sach vdi ImL PBS. Dich dugc Igc cho vao dng eppendorf, ly tam 2500 vdng/phiit ttong 5 phut d 37°. Loai bd dich ndi, cho them 1 mL PBS, ldc dlu, ly tam ldn 2, 2500 vdng/phiit ttong 3 phiit d 3TC. Loai bd dich ndi va cho vao 0,5 mL DMEM/F12, 10% FBS, 1% antibiotic/antimitotic, 1% L-glutamine va 1% penicillin/sti^ptomicin [12],
Xdc dfnh ty li sdng chit cua ti bdo: Igc va ly tdm, dSm sd lugng te bdo vdi thulc nhupm Trypan Blue: 20 pL dich tteo tS bdo ttdn vdi 20JJL Trypan Blue 0,4%, t l bdo khdng bat mau dugc dim tren budng dlrti hdng cdu [12].
Nudi cdy vd xdc dinh ddc tinh ti bao sau nuoi cdy: Sau khi xdc dinh sd lugng, tl bdo dugc cho cac binh nudi, them 3 mL mdi tiudng va nuoi d nhiet dg 37°C ttong 24h vdi mdt dp nudi 50.000 t l bdo/cm^. TiSn hanh nudi cdy so cap va cay chuyin tang sinh dl danh gia hieu qua phdt triSn tS bdo.
Khi qudn till te bao trung md tuong ddi ddng nhdt, cdn kiSm tta dac tinh tS bao bdng each bi?t hda thdnh tS bdo xuong, sun hay mo. Nudi tS bao vdi DMEM/F12, 10%
FBS cd bd sung: 0,5 mM IBMX, 50 pM indomethacin va 0,5 pM dexamethasone. Sau khoang 1-2 tuan nudi cay ttong mdi trudng biet hda, t l bao dugc cd dinh bang formandehyde 10% ttong 30 phut va nhugm vdi tiiudc nhudm Oil red-O 0,5% it nhat 60 phiit d nhiet do phdr^ [5]. Oil red-O la thudc nhudm lipid, khi nhugm mdu tS bao co djnh, Oil Red-O se hda tan cdc gipt md va lam chimg cd mdu do.
Thdng kevdxuly sd lieu: xxx ly bang chuang trinh Microsoft Excel 2010.
H I . K E T Q U A N G H I E N ClTU
Ndng do collagenase va thdi gian u collagenase sir dung cho qua trinh phan giai md la hai ySu td quylt dinh hieu qua phan lgp tS bao dan nhan ciing nhu su phat tiien te bao in vitro, vi vay chiing tdi tap tnmg nghien ciiu vdo 2 ngi dung nay.
3.1. Ket qua phan l^p va nudi cay te b^o v61 cac nong dd collagenase khac nhau TiSn hanh thu man 5 dgt, chugt cd khdi lugng trung binh la 56,23 ± 0,73 g. Chiing tdi triSn khai hai Id thi nghiSm vdi ndng dp collagenaselmg/mL vd collagenasel,5 mg/mL Sau loc, ly tam, dim sd lugng t l bao vdi Trypan Blue (Hinh 3.1), sd lugng te bao chet (bdt mau xanh tim) la rat it, cho thay collagenase hieu qua cho viec phan lap tS bao don nhan tir md.
KSt qua phan \^p tS bao don nhan cd su khac nhau giiia hai ndng dd collagenase 1 mg/mL va 1,5 mg/mL. Sd lugng t l bdo don nhan thu dugc ty IS thuan vdi nong dp collagenase. So te bao dan nhdn thu dugc d ndng do collagenase Img/mL: 4,24 xlO^ ± 0,33 xlO^ t l bdo/g va d ndng do collagenase 1,5 mg/mL: 5,22 xlO^ ± 0,52 xlO^ t l bao/g.
Tuy nhiSn d nong dp collagenase 1,5 mg/mL mac dii phan lap dugc sd lugng te bao don nhan nhiSu ban nhung van cd mdt vai t l bao chit. Trong khi dd, d ndng d^ collagenase 1 mg/mL, phan lap dugc sd lugng t l bao don nhan it hon nhung hdu nhu khdng cd t l bao chit (Hinh 3.1).
374
TAP CHI Y Dirge Hpc CAN THg - SO 11-12/2018
Hinh 3.1. Cac te bdo doTi nhan quan sat trSn buong dem du'di vat kinh X40 khi u md m& vol enzyme collagenase 1 mg/mL (A) va 1,5 mg/mL (B)
(miii tSn xanh: t l bdo don nhdn, mUi tSn dd: tS bao chet)
Sau khi phan lf.p tS bdo don nhan, chiing tdi tiSn hdnh nudi tang sinh tS bdo in viti'o dS danh gia hiSu qud phat triln t l bao d hai ndng dp collagenase Img/mL va 1,5 mg/mL.
+ Nghiem thiic 1: ndng dp collagenase 1 mg/1 mL+ DMEM/F12.
+ NghiSm thiic 2: ndng dp collagenase 1,5 mg/1 mL+ DMEM/F12.
KSt qua ddnh gia kha nSng phdt triln t l bdo qua qud trinh nudi in vitro cho thay te bao phat triSn d ca hai nghiem thiic dlu dat trSn 50%. KSt qua dugc tiinh bay d Hinh 3.2 va 3.3, cu thS kha nang phat ttiln t l bdo don nhan trong nudi cdy in vitro cd sir khdc biet thdng ke giiia hai nghiem thiic: d ndng dd collagenase 1 mg/mL, sd lugng te bao phat tiiln dat 64,85 ± 3,86% va ndng dg collagenase 1,5 mg/mL, s6 lugng te bao phat trien dat
54,42 ± 3_,_5_3%. __ _ _ _ _ _ „ _ _ _
' 7 — - - i
l | >-
I
Hinh 3.2. Anh hudng ciia nong dp collagenase den sd lugng te bao don nhdn
Hinh 3.3. Anh hudng cua ndng do collagenase dSn hiSu qua phat trien
tl bao sau nudi cay
Khi so sanh hieu qua phdt triln t l bao d hai nghiSm thiic chung tdi nhan thay, d nghiSm tinic 2 tuy thu dugc sd lugng t l bao don nhan cao ban nghiSm thiic 1: 18,78%
(tuong iing 9,8x10* te bao/g), nhung hiSu qua phdt trien t l bao sau nuoi cdy d nghiem thiic 1 lai cao hon nghiem tinic 2: 10,43%.
Tir kit qua thu dugc chiing tdi nhdn dinh ndng dd collagenase Img/mL la ndng dO thich hgp cho viec phan l$p vd tang sinh t l bao tnmg md tir md. Khi ii md mo vdi ndng
TAP CHI Y DUgC HQC CAN THg - s 6 11-12/2018
dg collagenase 1,5 mg/mL, ndng dg nay qud ngudng dS cat cac cau noi liSn bao, cac tS bao bi tdn thuong va giam bo^c mat khd ndng phuc hoi trong qud trinh nudi cdy tiSp theo.
3.2. Ket qua phdn lap va nuoi cay te bao vdi cac thM gian u collagenase khdc nhau Tren ca sd kit qua tiiu dugc d nong dp collagenase Img/mL cho hieu qua phat triln t l bdo cao: 64,85 ± 3,86%, chiing tdi chgn ndng do collagenase 1 mg/mL d l tilp tuc nghiSn ciiu kha nang phat ttiln tS bdo vdi thdi gian u collagenase khdc nhau.
Tiin hanh thu mdu 5 dgt, chugt cd khdi lugng tirung binh la 55,15 ± 0,71 g. Cdt nho 1 g md mo, cho vdo 1 mL DMEM/F12 cd collagenase ndng dd 1 mg/mL, hai nghiSm thiic vdi hai tiidi gian u khdc nhau dugc thuc hien:
+ Nghipm tiiirc 1: 30 phiit, 37" C + Nghiem thiic 2: 50 phiit, 37" C
Kit qua dugc tiinh bay d Hinh 3.4 va 3.5 cho thdy: 6 nghiSm thiic 1, vdi thdi gian li 30 phiit, sd te bao don nhdn thu dugc: 3,93x10^ ± 0,38x10^ tS bao/g va sd lugng t l bao phat ttiln dat 62,99 ± 2,35%. 0 nghiem tinic 2, vdi thdi gian ii 50 phut, so t l bdo dan nhan thu duoc: 4,77x10^ ± 0,31x10^ t l bao/g va sd lugng t l bao phat triln dat 69,33 ± 1,49%.
I
^^^^^•!,Hinh 3.4. Anh hudng cua thdi gian u den sd lugng t l bao don nhan
Hinh 3.5. Anh hudng ciia thdi gian li dSn hiSu qua phdt triSn tS bdo sau nudi cay Khi so sdnh hai nghiSm thiic nhan thdy, nghiem thiic 2 cd kSt qua cao ban nghiSm thiic 1, cu till, sd lugng te bao don nhan cao hon 17,61% (tuong iing 8,4x10" t l bao/g) va hieu qua phdt trien tl bdo sau nudi cdy c ^ hon 6.34%.
KSt qua phdn lap te bdo trung md tir mo chupt cua chung tdi la thdp ban so vdi mdt sd nghiSn ciiu cua Yang (2011), d^t: IxlO^ t l bao/g [14] vd Banas (2007), dat 4,6x10 tS bdo/g khi tdch tir md mo ngudi [4]. Tuy nhien khi so sdnh vdi cdc ngudn thu te bdo trung md khdc nhu tiiy xuong trong nghiSn ciiu ciia Meirelles (2003), dat 4,5x10^ tl bao/2 mL tuy xuong [11], kit qud nay thdp hon so vdi kit qua chung tdi thu dugc:
3,93x10^ va 4,77x10^ tS bao/1 g mo.
Tir cac kSt qua thu dugc chiing tdi nhan thdy, cac mo md ttdng dudi da la nguon khai thac tS bao trung md cd thS thay cho ngudn dich tuy xuong, ddy rdn. Phan lap te bdo don nhan vai ndng do enzyme collagenase 1 mg/mL va li 50 phiit cho kit qua cao. Tit dd, chiing tdi tiSp tuc nghiSn cim, ddnh gid cdc dac diim cua t l bao ttong qua trinh nudi cdy vd biet hda in vitro cua MSCs.
376
T»P CHl Y Di/ac HQC C&N THff - s6 11-12/2018 3.3. B^c aiem hinh thai t^ bao trong nuoi cay in vitro
3.3.1. Ket qua nuoi c^y so cap
Cac te bao don nhan sau khi thu nh$n duac nuoi cay trong moi truong DMEM/F12,10% FBS, 1% ABAM, 1% L-glutamine va 1% penicillin/streptomicin.
Sau 24 gio, cac te bao bam. Sau 72 gio, t6 bao trai, co hinh dang hinh thoi dac trung. Sau 5-7 ng4y nuoi ciy, te b4o hpp dong, trai rong va tang sinh. Sau 10-15 ngay nu6i cay, m|t do tS bao dat 70-80%, tiSn hanh cdy chuyk.
IW
Hinh 3.6. Ket qud nudi cdy chgn lpc te bao (X40)
A. TS bdo dugc thu nhan; B. Sau 24 gid nudi cay, tl bao bdm dinh vdo bl mat binh nudi; C. Sau 72 gid nudi cdy, te bao ttai ddi vd tang sinh; D. Sau 10 ngdy nudi cay, hau
hSt tS bao cd dang hinh thoi dac tnmg va tang sinh manh
So vdi nhiSu nghiSn cim trudc ddy nhu nghiSn cim cua Yang (2011) khi tach tii md ngudi [14], Niyaz (2012) khi tdch tir md chugt [13], Mohammadi-Sangcheshmeh (2013) khi tdch tir ma lac dd [12], thdi gian tl bdo bam va ttai cua chiing tdi cham ban.
Sau 72 gid quan sdt trSn kinh hien vi, cac te bao ttai vdi hinh thai dac tnmg (gidng nguyen bao sgi), nhu vay te bao chiing tdi thu dugc sau nudi cay la te bao tnmg md.
3.3.2. Ket qua cay chuyen tang sinh
Khi bl mat binh nudi khdng cdn dien tich dl cdc tS bao phat triln, cay chuyin de cung cap khong gian cho tS bao phat trien. Sau 24 gid, tS bao bat dau bam dinh va trai dang hinh thoi dac trung va tang sinh. Sau 4-5 ngay, te bao hgp ddng, trai dSu trSn bS mat binh nudi va tiep tuc tang sinh manh.
Hinh 3.7. Ket qua cay chuyen tang sinh (quan sat d v$t kinh X40) A. T I bdo dat miit dd cay chuySn (70-80%); B. TS bdo khi vira dugc cay chuyen;
C. TS bdo bam va trai sau 24 gid cdy chuyin; D. T I bdo tang sinh m ^ sau 5 ngdy cdy chuySn
3.4. Biet hoa tl bao trung mo thanh tl bao tao md'
Sau 24 gid khi tl bao da bam, cdn bd sung chdt cdm iing biet hda. Theo doi tir 24 gid din 2 tudn, nhan thay cd sy: thay d6i hinh dang ciia tS bao khi nudi ttong mdi trudng biet hda: tl bao tir dang thoi dai ddn ddn co ttdn lai. Sau 4-5 ngay, bdt ddu xudt hien cac tS
TAP CHI Y Dl/gC HQC CAN THg - SO 11-12/2018
bao mo nhd ddn tich tu ttong tS bao chat, cac gigt mo nay ddn ddn gdp thanh gipt mo va chiim gdn hit thi tich t l bao. Khi nhugm vdi thudc nhudm Oil red-O, chiing bdt mau dd. Tien hanh nhudm d ngay thii 10 sau khi nudi ttong mdi trudng biet hda.
Khi so sdnh vdi tS bao ddi chirng (nudi ttong mdi trudng DMEM/F12, 10% FBS vd khdng cd chdt bam img biet hda), cdc tS bdo khdng thay ddi hinh dang vd khdng bdt mau thudc nhudm Oil Red-O (Hmh 3.7).
Hmh 3.8. KSt qua biet hod MSCs (quan sdt d vdt kmh X20) A. Cac tS bdo d Id ddi chiing, khdng bd sung chat c&n iing biet hda; B. Cac tS bao tao mo: sau 4-5 ngay ttong mdi trudng biet hoa tao md, cdc tS bdo tao mo vdi hinh dang khdi ttdn xudt hien; C. Cdc gipt md bat mau do khi nhudm vdi Oil Red-O sau 10 ngay cam ting biet hda
KSt qua nghien ciiu cua chung tdi la phu hgp vdi n ^ i e n ciiu cua Yang (2011) [4]
va nhiSu nghien ciiu khdc [2] [1].
IV. K E T LUAN
Tir nhitiig kSt qud nghien ciiu tten chung tdi nit ra ket luan:
- 6 nong dp 1 mg/mL ttong 50 phiit, sd tS bao don nhan thu dugc: 4,77x10 tS bao/g va sd lugng te bao phat triln dat 69,33%. p a y la kit qua tdt nhdt.
- O ndng dd collagenase 1,5 mg/mL, sd te bao don nhan thu dugc: 5,22x10 te bao/g va sd lugng t l bao phat triln d^t 54,42% cho thdy ndng do collagenase cao da lam anh hudng din khd ndng hdi phuc t l bao, dan din giam kha nang phdt ttiln ttong qua tiinh nudi cdy in vitro.
- Nudi cdy so cdp: t l bdo don nhan bdt ddu bam sau 24 gid, ttdi sau 48 gid. Sau 10-15 ngay nudi cay deit mat do cdy chuySn (70-80%), cd d ^ g hinh Ihoi dac trung.
- Nudi cay thii cap: te bao bam va ttai dang hinh thoi sau 24 gid nudi cay va tang sinh manh, sd lugng t l bao phat tiiln dgt 69,33 ± 1,49%.
- Biet hda tS bao thanh t l bao tao md: Cac t l bao tao mo xudt hien sau 4-5 ngay khi nudi ttong mdi trudng cd chat cam img biet hda. Cac t l bdo nay bdt mau dd khi nhugm vdi thudc nhupm Oil Red-O d ngay thii 10. Cac k i t qua nay da khdng dinh cac te bao thu dugc la t l bao goc trung md.
378
T»P CHl" Y DLf PC Hpc CAN THP - SO 11-12/2018 TAI L I E U T H A M K H A O
1. Arana M., et al. (2013), "Adipose tissue-derived mesenchymal stem cells: isolation, expansion, and characterization", Cellular Cardiomyoplasty: Methods and Protocols, pp. 47-61.
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3. BaerP.C. and GeigerH. (2012), "Adipose-derived mesenchymal sttomal/stem cells: tissue localization, characteri2ation, and heterogeneity", Stem cells international. 2012.
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from sttomal cells to stem cells and vice versa", Transplantation. 87(9S), pp. S36-S42.
7. Frese L., Dijkman P.E., and Hoerstrap S.P. (2016), "Adipose tissue-derived stem cells in regenerative medicine". Transfusion Medicine andHemotherapy. 43(4), pp. 268-274.
9. Kim N, and Cho S.-G. (2013), "Clinical applications of mesenchymal stem cells". The Korean journal of internal medicine. 28(4), pp. 387-402.
lO.MaUam E., et al. (2010), "Characterization of in vitto expanded bone marrow-derived mesenchymal stem cells from patients with multiple sclerosis". Multiple Sclerosis.
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isolation, in vitto expansion, and characterization", British journal of haematology.
123(4), pp. 702-711.
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13.Niyaz M., et al. (2012), 'Isolation, cuhuring and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique", Turkish Journal of Biology. 36(6), pp. 658-664.
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(Ngdy nhgn bai: 10/11/2017- Ngay duyet ddng: 08/01/2018)
BON HOP C H A T P H A N LAP TU" T H A N C A Y BBVI BIP CLINACANTHUS NUTANS (BURM. F.) LINDAU, A C A N T H A C E A E
Nguyin Thi Trang Ddi'*, Phan Nggc Vdn Anh', Huynh Nggc Thu/
I. Trudng Dgi hgc YDugc Cdn Tha 2. Bgi hgc YDugc TP. Ho Chi Minh 'Email: dai ngiryenthiti-ang(^yahoo.com
TOM T A T
Dgt v^n di: Sim hip tir Idu da dugc xem la vj thudc eo truyin a Thdi Lan, Indonesia, Malaysia. Y hgc ddn gian cua cdc nude dd ghi nhgn, Bim bip cd tdc dung chdng oxy hda. khdng khudn, khdng viem, trj cdn trimg^ eon, sdt, ban da, ly. ddi thdo dudng. Muc tiiu nghiin ciru: Phdn lgp mgt sd ftpp ehdt tir dich chiet thdn Bim bip vd xdc djnh cdu true cdc hgp chdt ndy. Doi tugng
