TAP CHi Y HOC VIET NWyi TAP 454 - THAHG 5 - 56 2 - 2017 Tuy nhien, khdng ed diem cut-off nao cd do nhay
100% va dp dac hieu 100%, do dd diem cut-off cua phCG dffdc xac dinh bdi dffdng eong ROC rat hffu ich nhffng khdng phai la chinh xae tuyet dol 100%^trong Hen Iffdng ket eye thai ky.
Oiem eut-off cua nong dp phCG tien Iffdng thai sinh hda la 115,5mIU/mL, vdi dp nhay 76%
va do 95%. Nghien cffu eiia tac gia htamdoh A.
Eskandar (2011): theo doi 261 ehu ky ICSI/IVF CO dffdng tinh vdi test phCG sau chuyen phdi tim dffde diem eut-off tien Iffdng cho thai sinh hda la 76,15mIU/mL, vdi dp nhay 93,33%, dp dac hieu 89,84% va dien tich dffdi dffdng eong ROC la 0,919 [5].
V. K ^ LUAN
- Ndng dp p-hCG trung binh cua thai lam sang la 761,5 ± 706,33mIU/mL va thai sinh hda la 161,02 ± 82,35mIU/mL.
- Diem eut-off nong dp phCG sau 14 ngay chuyen phdi tien lffdng eho thai lam sang la 299,5 mlU/mL, vdi dp nhay la 8 1 % va dp dac hieu la 98%; tien lff<^g cho thai sinh hda la 115,5 mlU/mL vdi do nhay 76% va dp dac hieu la 95%.
- Nong dp phCG ngay 14 sau ehuyen phdi rat cd y nghTa trong viec tien Iffdng, cung nhff theo dol ket cue thai nghen.
TAI Ll|U T H A M KHAO
1. B.Hedon, H.Dechoud, TAnahogy, RAmal and C.Humeau (1998), "Assisted procreation. Infertility and contraceptio/7" a textbook for dinical practice.
i.. Neeta Singh, Manu Goyal, et al (2013).
Predictive value of early semm beta-human cJiorionic gonadofrophin for the successful outcome in women undergoing in vitro fertilization. 3oumal of Human Reproductive Sdences, 6 (4), 245-247.
3. Maysa M. Khadra, Mazen A. Freij,etal (2016).
Human chononic gonadotropin cutoff value determined by receiver operating characteristic cun/e analysis is usefiil but not absolute for determining pregnancy outeomes. Middle East Fertility Society 3oumal, 21(2), 120-124.
4. Xiao-yan HUANG, Yuna ZANG, Hai-qin YU (2007). Serum beta hCG of 11 day after embryo transfer to predict pregnancy outcome. Journal of Reproduction and Contraception 18(3):213-219 5. Eskandar MA, Al-Shahrani M, Shaamash A, El-
Emain M, Al-Ahmad M, Payodon B (2001). Early Maternal Serum p-human Chorionic Gonadotropin Measurements After ICSI mthe Prediction of Long- term Pregnancy Outcomes. A Retrospective Cohort Analysis. J Clin l^ed Res,3(l):30-S.
6. S. Bjercke, T. Tanbo, P.O. Dale, L. Morkrid, T.
Abyholm. Human chorionic gonadotrophin concentrations in early pregnancy after in-vitro ferthzation. Hum Reprod, 14 (1999), pp. 1642-1646.
/ . P. Poikkeus, V. Hiilesmaa, A. Tiitinen. Serum HCG 12 days after embryo transfer in predicting pregnancy outcome. Hum Reprod, 17 (2002), pp.
1901-1905.
XAC DINH eOC TINH CAP VA DANH GIA KHA NANG GAY KICH U'NG DA VA MAT CtlA CHE PHAM CHIET BANG ETHYLACETAT
TU" CAY BABET LUN TREN DONG VAT THITC NGHIEM Phan Thi Hoa', Pham Thi Van Anh\ IVIai Phiromg Thanh^
TOM TAT
Muc tieu: Nghien cffU du'dc thffc hien nhSm xac flinh dpc tinh cap va danh gia kha nang gay kfch ffng mSt va da cua che pham chiet bang ethylacetat tff cay Babet liin tren dpng vat thifc ni^hlem. Phu'dng phap:
(1) Xac d|nh LD50 ciia che pham chiet tff cay Babet theo dffdng tiSm dffdi da tren chupt nhlt trin^ theo [rfiu'dng phap Litchfield - Wilcoxon va hffdng dan ciia To chffc Y tg The gidi. (2) Danh gia kha nang gay kich Cihg mlt va da cua che pham chiet tff cay Babet tren tho theo hffdng dan cua OECD va ISO 10993-10. Boi thudc thff len da thd, danh gia tinh trang ban dd va 'Hpci^in Ydddccdtm^n Wit Nam
'Tn/dng Bai hgc YHa Ngi Chiu b-ach nhiem chi'nh: Phan Thi Hoa Email: [email protected] Ngay nhan bai: 16.01.2017 Ngay phan bien khoa hgc: 18.4.2017 Ngay duyet b4i: 26.4.2017
phu ne tren da, xac dinh chi sd kich ffng da. Mau thudc thff du'dc nhd vao tui ket mac mat tho, danh gia cac tdn thffdng xuat hien tren giac mac, mong mat va ket mac de xac dinh mffc do kich ffng mlt. Ket luan:
Che pham chiet ethylacetat tii cay Babet liin it co dpc ti'nh cap vdi LD50 = 7,854 (8,924 - 5,070) gam dudc lieu/kg va chi so dieu tri la 163,625, cd kha nang gay ki'ch uhg da mffc dp vffa va gay kich iiTig mat mffc do nang.
T& khoa: ethylacetat, Babet lun, kich irng, dpc tinh cap
SUMMARY
EVALUATION OF ACUTE TOXICITY I N MICE, AND ACUTE SKIN AND EYE IRRITATION I N RABBITS OF ETHYLACETATE EXTRACT OF
MALLOTUS NANUS This study was aimed at evaluatingacute toxicity, acute skin and eye irritation ofethylacetate extract of l^aliotus nanus. Toxicity of the preparation was evaluated in S w i ^ albino mice by administering orally
l-£- MAT •^V^f aded doses of the extract in the ranges of 1.513g to
;.l33g /kg body weight to the animals and observed intinuously for the 72h. The median acute toxicity ilue (LD50) of the extract was determined to be 854g /kg body weight, and the therapeutic index as 163.625. In qrder ta determine whether the hylacetatefraetionof Mallotus nanu^asacute skin
•itation on NZW rabbits, several skinreactions were 'aluated.The skin of rabbits was treated with the test imple and whether the animals sustained significant un damage was determinedby observing sldn ythema and edema following the administration of le test samples. The average score was calculatedby Wing the scores for edema formation according to le primary skin irritation index (primary irritation dex, PII). Tlie results from the present study iggest that the ethylacetate fraction of Mallotus anus has moderate skin irritation. The eye irritation :st is carried out by applying Mallotus nanus ihylacetate extract in the conjunctival sac of one eye ' rabbits. The grades of ocular reaction, that is, jnjunctivae, cornea, and die iris, are recorded at ach examination as per OECD T6405. The ihylacetate extract of Mallotus nanus showed severe /e irritation properties.
Keywords: ethylacetate, Mallotus nanus, ritation, acute toxicity
OAJ VAN OE
Trffng ca la mdt benh ngoai da do rdi loan bat lffdng trong ddn vi nang Idng tuyen ba [ I j . I^ae u khdng gay bien ehffng nguy hiem, nhu'ng enh thffdng dien bien keo dai, dae biet ed the e lai seo Idm hoae seo ldi lam anh hffdng quan
•png ve mat tham my khien ngffdi benh mat tff n, anh hffdng den nang suat lam viec va chat fdng cudc sdng eiia ngffdi benh [2]. 6 Viet lam, da ed mdt so thude Yhpc cd truyen dffdc ung tren lam sang de tn mun trffng ca theo inh nghiem dan gian nhffng chffa thffc sff dffdc hffng minh tac dung. Cay thudc Babet liin Mallotus nanus) la loai cay dang dffde sff dung leojcinh nghiem dan gian d cac l»c ngffdi ttiieu 5 de lam dep da^ chffa benh trffng ea, ehu^ cd ong sach eua Do Tat Ldi va Vo Van Chi. Mdt sd ha khoa hpe d vien Hda ket hdp vdi Bi va Han
!u6c da nghien cffu thanh phan hda hpe va tae ung chong oxy hda eua la cay nam 2010 [3].
uy nhien, cho tdi nay tren the gidi chffa cd mdt 3i lieu nao edng bd ve thanh phan hda hpc va jc dung sinh hpe eung nhff b'nh an toan eua re eay ay. Nghien effu dffdc tien hanh nham muc tieu;
'ac dinh ddc b'nh cap va danh gia kha nang gay ich &ng da va mat ciia che pham chiet etfiylacetat '/cay Babet liin bin ddng vat Uii/c nghiem.
. DOI TU'ONG VA PHU'aNG PHAP NGHIEN CUU 2 . 1 . Dol tu'dng nghign cu'u: Che pham hiet bang ethylacetat tff cay Babet lun. 1 gam
ehu^ 22,7g dffdc lieu. Dung mdi pha thudc trffdc khi dung cho dpng vat thi nghiem: DMSO 5%.
2.2. Hoa cliait va dung cu phuc vu nghien cii'u: Gae vd khuan cat mieng hinh vudng diSn tieh 2,5em x 2,5cm, gae bang, nffde sach de rii^
sach vung tioi thuoe, proparaeain hydrochlorid 0,5%, kfnh lijp
2.3. Dong vat thu'c nghiem
- Chupt nhat trang, ehung Swiss, ca hai giong, khde manh, trpng Iffdng 20 ± 2g, da Vien Ve sinh djch te Trung ffdng.
- Thd chiing New Zealand White, long trang, trffdng thanh, khde manh, can nang 2,0 - 2,2k9 do Trung tam ehan nuoi dpng vat thi nghiem Ha Tay cung cap.
2.4. Phu'dng phap nghien cu'u 2.4.1. Nghien c&u doc b'nh cap: Xac dmh LDH cua cac che pham chiet tff eay Babet theo dffdng tiem dffdi da tren ehudt nhat trang theo phffdng phap Litchfield - Wileoxon va hffdng dan cua To c h f f c Y t e T h e g i d i [ 4 ] , [5]
2.4.2. Nghiin c&u kha nang gay kich uhg da:
Nghien cffu kha nang gay kfch ffng da dffde tien hanh dffa tren hffdng dan cua OECD va ISO 10993-10 [6],[7]^
- Chuan bi mau thudc th&: pha mau ehe pham chiet vdi DMSO 5% tao thanh dung djch cd nong dp 8,8mg eao/0,5mL (tffdng dffdng'0,2g dffdc lieu/0,5mL)
'Quy trinh nghien c&u: Trffdc ngay nghien cffu 24 gid, thd dffdc cao Idng d phan Iffng va hdng tren dien tieh 10 x 15cm d ea 2 ben cot sdng de bdi thuoe va quan sat eac vi tri thff nghiem. Oanh gia va tfnh diem cac chi so ve ban dd (erythema), phiJ ne (oedema) tai thdi diem 1 gid, 24, 48, 72 gid sau khi loai bd mau thff. Neu ed tdn thffdng, theo ddi thd 14 ngay de danh gia kha nang phuc hdi. Khi tdn thffdng da hoi phuc thi ngffng theo doi.
Bang 1. Bang danh gia tinh diem kich ffng da cho hai trieu chufng ban do va phCi ne
Ban d o
Phu ne
- Khdng cd ban
- Ban rat nhe (khd nhan thay) - D I nhan thay
- Nhe den nang
- Nang dai hinh thanh vay tren da - Khdng ed
- Rat nhe (khd nhan thay) - De nhan thay (da day len) - Trung binh (day len 1mm) - Nang (day hdn 1mm hoac
ra ngoai viing bdi)
Diem 0 1 2 3 4 0 1 2 3 4
TAP CHl Y HOC VIET NAM TAP 454 - THANG 5 - SO 2 - 2017 -Oanh gia ket qua: Tinh chi sd kieh ffng (PII:
pimary irribtion index) nhff sau:
+ Chi tfnh toan tff eac hien tffdng quan sat dffdc d cac thdi diem 24 gid, 48 gid'va 72 gid.
+ Tfnh chi so kfch ffng eho tffng thd-»Chi so kich ffng cua mau thff.
Bang 2. Bang xep loai kich u'ng da du'a vao P I I
- Mau thudc thff dffde pha vdi nong dp nhff trong nghien effu kha nang gay kfch ffng da. Sd Iffdng thd: 03 thd (danh sd tff 1 den 3) III. KET QUA NGHIEN CLTU
3 . 1 . Nghien ciiu doc tinh cSp: Can 0,5 gam cao can chiet ethylacetat tff Babet, pha trong dung dieh DMSO 5% thanh 15 ml vCfe du diJng trong nghien cffu ddc tfnh cap. Sau khi tiem thudc thff djch chiet ethylacetat tff 2mL/kg den lieu 20 miykg, tat ca chupt trong cac Id ed hien tffdng met, giam van dpng, khd thd, sau dd thay 2.5. Nghien cihi kha nang gay kich ii'ng ^^ ^ai ^^ q^an sat vimg niem mac. Xuat hien mat: Nghien cffu kha nang gay kieh irng mat chupt ehet d cac Id, so Iffdng chupt chet ly le thuan duac tien hanh theo hffdng dan cua OECD va vdi lieu dung va dffdc trinh bay d bang 4.
ISO 10993-10 [ 7 ] .
Bang 3. Moi tu'dng quan lieu Iu'dng va ty le phan tram chuot chet sau tiem can chiet ethylacetat tu* Babet
Xep loai Khdng kich ffng
Kich utiq nhe Kich ffng vffa Kieh ffliq nanq
P I I trung binh 0 - 0 , 4 0,5 - 1,9
2 - 4 , 9 5 - 8
Lo chuot 1 2 3 4 5 6 7 8 9 10
The tich tiem (mL/l<q) 2,0 4,0 6,0 8,0 10,0 12,0 14,0 16,0 18,0 20,0
Lieu (g du'dc lieu/l(q) 1,513 3,027 4,540 6,053 7,567 9,080 10,593 12,107 13,620 15,133
Chuot chet (%) 0 10 20 40 50 60 80 90 90 100
Tff dd tinh diicic LD50 eanchiet ethylacetat tff Babet: LDJQ = 7,854 (8,924 - 5,070) gam dffdc lieu/kg; TI= [(7,854/0,2) x 50]: 12 = 163,625 (Tinh ng&di tr&dng thanh 50kg, he sd ngoai suy tren diudtnhat 12). Chi so dieu trj ( T l : therapeutic Index) cdn dffdc goi la cffa sd hoac eCTa so dieu tri an toan, TI =LD5O/EDSO (trong nghien effli tren dong vat thffc nghiem)3.2. Nghien cuii kha nang gay kich u'ng da
Bang 4. Bang danh gia ban do, phu ne tren cac tho danh gia kich ijlig da cua san che phgm chigt ethylacetat tir cay Babet ICin
Ban do Phil ne
Tho 1 qid I 2 4 old I 4 8 aid 7 2 gid 1 aid 24 gid 4 8 aid 72 gid M l Ch M l Ch M l Ch M l M l Ch M l Ch M l Ch M l Ch Thd 2
7110 3
Ml: vimg da t}di mau 1 nong do 0,2g d&de imu/0,5 Mi; Ch: vung da t}6i dung mdi DMSO 5%
Ket qua d bang 5 cho thay:
- 6 tat ca cac thd, khdng xuat hien hien tffdng ban dd hay phu ne tren viJng da bdi DMSO 5%
dung lam ehffng.
-Tren vijng da bdi thude thff tffdng dffdng iieu 0,2 g dffcfc lieu/0,5 rnl:
+ Thd so i : d thdi diem 1 gid sau khi loai bd thudc ra khdi viing da, khdng ed hien tffdng ban
0
dd. O cac thdi diem 24 gid, 48 gid va 72 gid deu cd ban dd de nhan thay. Khdng quan sat thay hien tffdng phii ne d eae thdi diem.
+ Thd so 2: d thdi diem 1 gid sau khi loai bd thudc ra khdi viing da, khdng cd hien tffdng ban do. 0 thdi diem 24 gid, ed hien tffdng ban dd mffc dp vii^. a thdi diem 48 gid va 72 gid deu ed ban do de nhan thay. Khdng quan sat thay hien tffdng phiJ ne d cae thdi diem.
+ Thd sd 3: d thdi djem 1 gid sau eo ban dd Iffng khd nhan thay. 6 thdi diem ^ 4 gid va 48 id ed ban dd de nhan thay. Thdi diem 72 gid ehi ]n ban dd khd nhan thay. Khdng quan sat thay ien tffdng phii ne d eac thdi diem.
Bang 5. Chi so kich u'ng ( P I I ) trSn tho danh gia kich ihig da ciia che pham chiet thylacetat td* cay Babet lun
Tren ca 3 thd, hien tffdng ban dd deu mat di va da thd hoi phue sau 5 - 7 ngay.
Tff ket qua tren tinh dffdc ehi sd kieh utig PH tren tffng thd nhff bang 6.
Thd sd 1 2,00
Thd 50 2 2,33
Thd sd 3 Q 1,67 ^ Tff ket qua d bang 6, tfnh dffdc chi sd kich ffng PII eiia mau thude thff vdi nong dp 0,2 g dudc
|u/0,5mL la: PII = (2 -l- 2,33 + l,67)/3 = 2.
Du^ vao bang phan loai kieh ffng da theo PII, ehe pham chiet ethylacetat tff cay Babet liin v6i ong dp 0,2 g dffde lieu/0,5 mL gay kieh ffng da mffe dp vffa.
3.3. Nghien cuii kha nang gay kich u'ng mat
Bang ff. Bang danh gia kich iing mat cua can chifi't ethylacetat tii Babet Tho
Tho so 1
Thdi diem IqicI 24 qid 48 qid 72ql6
Mu'c do md due giac mac
0 2 3 3
Mong mat
1 1 1 1
Ket mac Mu'c do do
2 2 2 3
Phu Icet mac 1 3 4 4 Ket qua d bang 7 eho thay: Khi thffc hien kich
'ng mat tren thd sd 1, ngay 1 gid sau khi nhd liudc xuat hien tdn thffdng mdng mat, ket mac lffe dp 1 va 2.
-Tai eae thdi diem 24 gid, 48 gid va 72 gid, uat tiien eac ton thffdng md due giac mac, tdn hffdng mdng mat, mffe dp do, phii ne ket mac igay eang nang them.
Sau 72 gid tiep tue theo ddi, danh gia mffc dp a tien trien eua eac ton thffdng d mat thd thi hay cac tdn thffdng ngay eang nang. Oen thdi tiem sau 6 ngay nhd thuoe, xuat hien md due jiac mac dp 4 (glae mac trang due, khdng thay hi tiet mong mat). Day la mdt dau hieu kich ffng nat nang.
V. BAN L U A N
4 . 1 . Doc tinh cap: Cho chupt tiem dffdi da lay thudc thff theo lieu tang dan tff l,513g dffde eu/kg den 15,133 g dffde lieu/kg chupt, theo Idi bleu hien eiia chupt trong vdng 24 gid dau au khi tiem thuoc da quan sat thay cae dau hieu :ua dpe tinh bao gom giam van dpng, khd thd, au dd thay tim tai khi quan sat viing niem mac.
.leu l,513g dffdc lieu/kg chupt la lieu cao niiat hdng gay chet chupt, va lieu 15,133 g dffdc
^u/kg chupt la lieu thap nhat gay ehet chupt ,00% sau 24 gid quan sat. Tff do thj bieu thj mdi en quan tuyen tfnh giffa lieu Iffdng eua can :hiet ethylacetat tff Babet va ty le ehupt ehet (do
thi 1) da xac djnh dffde LD50 = 7,854 (8,924 - 5,070) gam dffde lieu/kg, va ehi so dieu tri ciia mau thude thff la 163,625. Theo hffdng dan ciia To chffc Y te the gidi ve thuoe ed nguon goc dffdc lieu vdi chi sd T l > 100, day la dffdc lieu it ed ddc tfnh cap. Pham vl an toan eua che pham chiet ethylacetat tff eay Babet lun dat yeu clu, lieu tac dung < 1/100 LDso [5].
4.2. Kha nang kich ii'ng da: Kha nang gay kfch ffng da eua can chiet ethylacetat tff cay Babet liin dffdc tien hanh bang each boi mau thudc thff vdi nong dp 0,2 gam dffdc lieu/0,5 ml len da tho. Sau 24 gid boi thuoc, cac viing da boi mau thuoc thff deu xuat hien nhuhg ban do de nhan thay, tuy nhien khdng ed hien tffdng phii ne d cae thdi diem quan sat. Cac ban dd tren da thd deu mat di va da thd hoi phue sau 5-7 ngay, Trong khi dd, cae vting da bdi dung moi pha thuoe la DMSO 5% deu khdng cd hien tffdng ban dd va phii ne. Oieu nay cho thay, hien tffdng ban dd tren da thd khi bdi thuoe thff khdng phai do DMSO 5%. Nhff vay, dau hieu kich ffng khi boi thudc thff la ban dd khong kem phii ne, trieu ehffng nay bat dau xuat hien rd sau 24 gid bol thuoe, va co the hoi phuc hoan toan sau 57 ngay ngffng sff dung thudc. Chi so kich ffng tren da thd PII la 2, dffa vao bang phan loai kfch iJhg da theo PII (bang' 2), che pham chiet ethylacetat tff eay Babet lim vdi nong dp 0,2 gam dudc lieu/0,5mL gay kich ffng da mffc dp vffa.
TAP CHl Y HOC VIET HAM TAP 454 - THANG 5 - SO 2 - 2017 4.3. Kha n3ng kich ii'ng mat: Kha nang
gay kich ffng mat diidc tien hanh bang each nhd 0,lmL mau^ thudc thff vao till ket mae mat phai ciia thd, mat trai khdng nhd gi. Oanh gia cac tdn thffdng giac mac, mong mat va ket mac tai cac Uidl diem 1 gid, 24 gid, 48 gid, 72 gid sau khi nhd thuoc. Ket qua eho thay, ngay 1 gid sau khi nhd thude thff da xuat hien eae tdn thffdng mong mat, ket mac mffc dp 1 va 2. Sau 72 gid, xuat hien cae ton thffdng md due giac mac, tdn thffdng mong mat, mffe dp dd, phii ne ket mac ro ret hdn. Tiep tuc theo ddi, danh gia mffc dp va tien trien cua cac tdn thffdng d mat thd den thdi diem sau 6 ngay nhd thude, xuat hien md due giac mac dp 4 (giae mac trang due, khdng Hiay ehi tiet mdng mat). Nhff vay, cd the thay, mau thude thff da gay tdn thffdng ea giae mac, mdng mat va ket mac, eac tdn thffdng xuat hien sdm va tien trien nhanh va ngay cang nang hdn.
Theo hffdng dan cua OECD, cd the thay, mau che pham chiet ethylacetat tff cay Babet lim gay kfch ffng mat nang tren thd.
V. K ! T LUAN
- Vi dpc tinh cap: pham vi an toan eua che pham chiet ethylacetat tir eay Babet tun dat yeu rau,lieu tae dung <1/100 LD50. Theo hffdng dan cua To ehffe Y te the gidi ve thudc cd ngudn gde duWc lieu vdi chi sd TI > 100, day la dffde lieu it cd dpc tfnh cap.
- Ve kha nang gay kich &ng da: che pham chiet trong ethylacetat tff cay Babet lim nong dp
0,2 gam dffdc lieu/0,5 mL eo ehi sd kich iing bang 2, xep loai kfch ffng da mffe do viici.
- Ve kha nang gay kich &ng mat: che pham chiet ethyl acetat tff eay Babet liin pha trong dung mdi la dung djch DMSO 5% tao thanh dung djch cd nong dp 0,2 gam dffde lieu/0,5 mL gay kieh ffng mat nang tren thd.
T A I UEU T H A M KHAO
1. Ray C, Trivedi P, Sharma V (2013). Acne and Its treatment lines. International Joumal of Research in Pharmaceutical and Biosciences.
3(1):1-16.
2. Zaenglein AL, Grabber EM, Thiboutot DM, Strauss JS (2008). Chapter 78: Acnes vulgaris and acneiform eruptions. Fitzpatrick's Dermatology In General Medicine, 7th edition. Newyork, USA:
McGraw Hill Companies. 690-702
3. Phan VK, Nguyen TM, Minh CV et al (2010).
Two new C-glucosyl benzoic acids and flavonoids from Mallotus nanus and their antioxidant activity.
Arch Pharm Res, 33(2):203-208.
1 . OECD (2001). Guidance Document on the Recognition, Assessment and Use of Qinical Signs as Humane Endpoints for Experimental Animals Used In Safety Evaluation, acute oral toxicity.
Environmental Health and Safety Monograph Serieson Testing andAssesmentNo 19.
5. World Health Organization (2000). Working gmup on die safety and efficacy of herbal medicine. Report of regional office for the western pacific of the World Health Organization.
6. OECD 404 (2015), OECD guideline for testing of chemicals: Acute Dermal Irritadon/Corrosion 7. International Organisation of
Standardization(2010}, Biological evaluation of medical devices - Part 10: Tests for irritation and skin sensitization, ISO 10993-10.
DIEU TRA MOT SO TRIEU CHU'NG BENH LY CUA DAN CU*
SONG TRONG M O T KHU CONG NGHIEP
Nguyin Dire Sffn*
TOIVI TAT
Nghien cffu dffdc tien hanh nham dieu tra mot so bleu hien tri^u ehffng benh ly tr§n 3428 ngffdi song trong cung mot khu dSn cu" tiiupc mot khu cong nghilp; trong do co 192 do\ tffdng phai tiep xuc vdi hoa chat trong khi lam viec. Tuoi ddi trung binh cua nhom tiep xuc la 41±8 tuoi va d nhom khdng tiep xuc la 49±12 tuoi. Ket qua dieu tra cho thay ty le cac doi
*]^en SOt khde nghe nghiep va Mdi trudng Oijii trach nhiem chinh: Nguyen Dffc Sdn Email: [email protected] Ngay nhan bai: 8.2.2017 Ngay phan bien khoa hoe: 10.4.2017 Ngay duyet bai: 28.4.2017
tffdng cd bieu hien kich thich eac he cd quan ciia ed the d nhdm cd tiep xiic deu eao hdn nhom khong tiep xuc CO y nghla thong ke (p<0,001). Cac tneu chuiig chfnh ve mSt (cay mat, chay nUdc mat 30,7% va 14,0%; ngffa mat 47,0% va 26,4%; do mat 9,4% va 3,4%), ve mui (cay mCJi, chay nffdc miji 30,2% va 15,8%; ngffa mui, hit hdi lien tuc 26,6% va 16,1%), vS hong (ngffa hong 52,6% va 35,7%; dau hong 55,2% va 34,1%); budn non 4,2% va 1,7%; tffe ngUc 16,1% va 7,4%. Tuy vay, khong thay c6 sff khac biet ve ty le benh tat giffa hai nhom doi tffdng nay (p>0,05). Nghien cffu cung tim tliay moi lien quan ro ret giffa cam nhan mffe do 6 nhiem moi trffdng song vdi cac bieu hien kich thich cac he cd quan cua ed the (p<0,001).
