Nghien CLFU - Ky thuat
cung d i o ttily, ham luang hoat c h i t adenosin va eonjycepin d p h i n ngpn d a nam DTHT Id cao han hdn so vdi d c phan con lai. Dieu nay, giup eho nha san x u l t va ngudi tieu dung d can c d de lya chpn dupe nguyen lieu d n ttiiet. phu hyp vdi ydu d u s u dyng.
Ve d f l u kiOn s i c ky, chung tdc can d vao cac lai ligu tham khao va b^ng t h i l t bj ca ban hien d d a cac phong thi nghigm de xdy dyng va t h i m djnh phuang phdp. & Viet Nam, d mpt sd nghien cdu dd d n g bd ve djnh luyng adenosin va cordycepin tiiDng DTHT. Nguydn Thj Que Mai va CS dd eong bd phuang phdp djnh luyng bdng HPLC d g y tiieo ehuang b'inh gradient vdi dung moi gdm MeOH vd nude voi thdi gian phan tich Id 40 phut. Thdi gian luu d a 2 c h i t ndy Um I h l y la 20,09 ± 0,010 vd 22,17 ± 0,012 phut f*'. Phuang phdp cua chijng toi s d dyng hd dung mdi Id MeOH vd dung djeh dgm phosphat pH 6,3 eho thdi gian x u l t hign pic id n g i n han nhiiu (8,480 ± 0,024 va 10,716 ± 0,03 phOl) cho phep till kigm thdi gian phdn llch vd dung moi chgy s i c ky md van ddp ung cdc ydu d u cua phdp dinh luyng.
K e t l u $ n
Da xdy dyng vd t h i m djnh d u y c phuang phdp
^ n h lupng adenosin va cordycepin ttong DTHT.
Phuang phdp dd xdy d y n g Id ftjong ttiich vdi hg t h i n g sdc ky-, ddm bao dd chpn Ipc ddc hlOu; dO dung (> 90%) vd dp chinh xdc eao (RSD < 2.0%).
Ap dyng phuang phap da xdy d y n g d ttdn d l phdn Uch ddnh gid hdm luyng 2 hogt d i t ndy d cde bO phan khae nhau cua nam DTHT. k i t qua d o ttily, phan ngpn n i m co ham lupng hogt c h i t eao han nhieu so vdi cde p h i n khac.
Tai Heu t h a m k h a o 1. BO Y t l (2009), Duac diin Vipt Nam IV, Nhd xult ban Y hpc, Hd Npi.
2. Dd T i t Lpi (2004), Nhung cdy thuSc vd vi thudc Vipt Nam, Nhd xult bdn Y hpc, Hd NOL ti". 978.
3. Duyc hpc c6 tmyin (2002), Nhd xult ban Y hoc.
Ha Npi.
4. Nguyin Thj Que Mai (2015), "Xdy dyng phuong phap djnh Unh, djnh luyng ddng tiidi adenosin vd cordycepin trong c h l phim chda d6ng trtng hg th^o bdng phuang ph^p sic ky long hidu nSng cao", Lu$n vdn Thpc sTDupc hpc. Trudng Ogi hpc Dupc Hd NOi.
5. M. G. Shashidhar etal. (2013), "Bioactive principles from Cordyceps sinensis: A potent food supplement - A review", Joumal offuncb'onal foods. Volume 5, Issue 3, pp. 1013-1030.
6. ICH (2005), Validalkm of analytical procedures:
text and methodology. Q2R1.
{Ngay nh$n bdi: 20/04/2016 - Ngdy duy$t dSng: 03/10/2016)
Phan lap moracin M, dihydromyricetin va cucumegastigman I tir la sa ke
(Artocarpus communis J. R. Forst & G. Forst)
Nguyen Thi Thiiy An, Lirong L? Thuy Thai Nguyen Hung Thu', Nguyen Th^i An
Trudng Dgi hpc Dirge Hd Ngi 'E-mail: tnhl22(fi}yahoo.com
S u m m a r y
Fmm the ethyl acetate extracts of the leaves of Artocarpus communis (J. R. Forst. & G. Forst), other three compounds were Isolated by various chromatography methods. Their structures were identified as moracin M (1), dihydromyricetin (2) and cucumegastigman I (3) by 1D-. 2D-NMR. and MS in refemnce to the literature. Compouds 1, 2 and 3 were dectected for the first time in the Artocarpus communis (J.
R. Forst & G. Forst)..
Keywords: Artocarpus communis J. R. Forst & G. Forst., moracin. dihydromyricetin.
cucumegastigman.
TAPCHI DlTgfC HQC - 10/2016 ( S 6 486 NAM 56)
• Nghien CIFU - Ky thuat
Dat van d e
Cay sa ke cd ldn khoa hpc Artocarpus communis, hp Ddu tdm - Moraceae, d u y c ttdng khd p h i b i l n d cde ti'nh m i l n Nam Vidt Nam. Trong dan gian, Id d y nay d u y c dung d l tri phu thung hay vidm gan vdng da, sdi thgn, tilu d u d n g , tang huylt dp, gout... 1^^.
G i n day, d y sa ke d u p c mOt so nha khoa hpc quan ldm nghien euu v l thanh p h i n hda hpc cung nhu tae dyng d u y c ly. T u phan dogn doroform d i d d i i l t la sa ke, Tran Thu Huang vd d n g s y dd phan lgp d u y c 9 hyp ehit '^'. Nhom nghien d u cua chung toi da tiln hanh tham d6 lac dyng tri gout d a la sa ke '^' vd ti!r phan dogn ethyl acetat djch chiet id sa ke da phan lgp d u y c 2 hyp c h i t Id kaempferol 3-0-njUnosid va byzantionosid B'^. Bai bdo ndy liep lyc cdng bd k i t qua phan lgp va nhgn dgng 3 hpp c h i l khac t u phdn dogn ethyl acetat cua la sa kd.
Nguyen li^u va phuxmg phap nghien ciAi Nguydn lidu
Ld sa kd dupe Ihu hdi tgi Qudn Thu Due - Thdnh phd H i Chl Minh vdo thdng 11 nam 2013.
M i u dd d u p c PGS.TS. Nguyin Khdc Khoi - Vign Sinh tiidi vd Tdi nguydn sinh vdt gidm djnh ten khoa hpc id Artocarpus communis J. R. Forst. & G.
Forst.. ldn ddng nghTa Id Artocarpus altilis (Park.) Fosb. hay Artocarpus Inclsus (Thunb.) L., hp Ddu tdm (MoraceaeJ.
Dupc ligu sau thu hdi d u y c n>a sgch, Idm nhd vd s l y khd d 50°C.
P h u a n g phap nghien c u u
S d dyng s i c kjf d t . s i c ky Idp mdng d i l u c h l d l phdn ldp cdc c h i t . Nhdn dgng sa bO cac c h i t b i n g phuang phdp sdc ky Idp m6ng. K h i n g djnh d u bTJc CLia cdc hpp c h i t phdn l§p d u y c d y a tren cdc d u lidu phd:
- s i c ky d t dupe t i l n hdnh vdi c h i t h i p phy silicagel c d hgi 40 - 60 pm vd chit h i p phg pha ddo YMC-ODS c d hgt 30 - 50 pm.
- Sdc ky ldp mdng d i l u c h l dupe thye hign Iren bdn mdng Silicagel GF^j^, phat hign v i t c h i l bdng dnh sdng h> ngogi d hai bude s6ng 254 va 366 nm hodc phun thudc tiiu Id vanllin/H^SO, 10%, ha ndng d l phdt hidn v i t c h i t . Sau do ego Idp silica gel cd d i l t nghidn edu, phdn hap phy va Unh c h l b i n g cdch k i t tinh Igi nhilu l l n trong dung mdi ttiich hyp.
- D i l m nong chdy dupc do tren mdy Kofler
micro-hostade. Phd khdi luyng (ESI-MS) d u o c do h^n mdy Agilent 6310 LC-MSD Trap, phd d n g hudng t u hat nhan (NMR) d u y c do Iren may Bmker AM500 FT-NMR Specttometer. Do diem chay vd do phd tgi V\^n Hoa hpc - Vidn Hdn ldm Khoa hpc va Cdng nghg Vigt Nam.
Ket qua va ban lu^n
Chiet xuat va phan lgp d c h p ^ chat La sa ke lam nhd (1000 g) d u y c cho vdo binh chiet inox, s d dyng dung m6i metiianol, ngdm d nhigt dp phdng, mt dich chiet 3 l l n (ttong 24, 48 vd 72 gid), Ipc, gOp d j d Ipc vd c6 d u d i dp s u i t giam thu d u y c d n toan phan AR (71,25 g). Hoa d n loan phan AR vdl m6l lupng vua dO nude ndng, s d dyng phuang phdp chilt Idng-ldng c h i l t l l n luyt vdi d c dung m6i Id n-hexan, doroform vd ethyl a t r i a l . Cdc phdn doan djch chiet d u y c d t tiiu hdi dung mdi d l d u y c d c d n l l n luyt Id AR-H (0,65 g), AR-C (6,22 g) vd AR-E (2,05 g). T i l n hdnh lap lgi mOt sd l l n chilt d l co d u p c luyng d e d n d n thiet.
Cdn ettiyl acetat AR-E (7,50 g) d u p c phdn ldp tten d t pha dao YMC-ODS, nJa gidl vdl hd dung mdi methanokiudc (1:1). Kiem tra b i n g SKLM, gpp d c dng ed d n g thdnh p h i n vd d l loai dung mdi d l d u y c 5 phdn dogn: AR-E1 (400 mg), AR- E2 (500 mg), AR-E3 (250 mg), AR-E4 (750 mg) vd AR-E5(1.20g).
Phdn dogn AR-E4 Ulp lye d u y c phdn ldp trdn d t silicagel. ed hgt 40-60 pm, n>a gial vdl hd dung mdi ethyl acetat - mettianol - acid formic (5:1:0,1).
K i l m tia ttianh p h i n cua djch n>a giai bdng SKLM d l ddn cdc dng co dJng Ihdnh p h i n , d t logi dung moi d l tiiu d u p c 3 phdn dogn AR-E4A (120 mg), AR-E4B (155 mg) vd AR-E4C (190 mg).
T u phdn dogn AR-E4C chung toi d§ phdn ldp d u y c 2 hyp c h i t '*>. Tiep tyc phdn ldp cac c h i t ttong phdn dogn AR-E4A ttdn d t silica gel, c d hgt 40- 60 pm, n>a gial vdl hg dung moi n-hexan - ethyl acetat (1,3:1) tiiu d u y c AR3 ( 1 , 6 mg).
Phdn doan AR-E1 d u y c phdn ldp tren d t pha ddo YMC-ODS, rda giai vdi hg dung mdi aceton- nudc (1:3). Kiem tta ttidnh p h i n cdc djch n>a gidl bdng SKLM, gOp d c dng cd cung tiidnh p h i n vd d t loai dung mOI d l d u y c 3 phan dogn: AR- E1A (11^0 mg), AR-E1B (30 mg) vd AR-E1C (50 mg). Tien hdnh sdc ky ldp mdng d i l u c h l vdi phan doan AR-E1B vdi hg dung mdi khai b i l n la didoHDmelhan-mettianol (5:1), ttiu d u y c AR4 (2, 6mg).
T ^ P C H i DU'OC HQC - 10/2016 (S6 486 N A M 56)
Nghien cifu - Ky thuat
Phan doan AR-E3 6iK?c phan lap tren cot pha d i o YMC-ODS, n>a g i i i vcri he dung moi aceton- nirdc (1:2). Kiem tra c i c djch n>a g i i i b i n g SKLM.
gop cac 6ng co cung ttianh phan v i c^t loai dung moi de dmjfc 3 p h i n doan: AR-E3A (10O mg). AR- E3B (70 mg) va AR-E3C (50 mg). P h i n doan AR- E3B Bip tgc diroc phan i i p tren cpt silicagel, c o hat 40- 60 pm. n>a giai voi he dung moi n-hexan - ethyl acetat (1,5:1)thu duwcARS (3,10 mg).
C i c h o p c h a t A R 3 ( 1 ) . A R 4 ( 2 ) v a A R 5 ( 3 ) d L i v c n h | n dang cau true di/a tren c i c dO lieu ph6.
Xae ^ n h cau true cae h o p ehat phan l i p 6irtyc
H</pchatAR3(1)
(1) l i bgt k^t t n h m i u vang. t,,= 261-263°C. tan treng methanol, ethanol.
Pho 'H-NMR eua hop chat (1) x u i t hien 3 tin hi§u proton d$c trung eua mOt v6ng thorn 5„ 7.37 (1H. d. J = 8.0 Hz); 6.76 (1H. dd. J = 8.0; 2.0 Hz) va 6.92 (1H. d. ,y = 2.0 Hz); tin hi#u mot vong thom k h i e CO tryc d6i XLmg cung duoe xae djnh tgi 5^
6.78 (2H. d. J = 2.0 Hz) v i 6.26 (1H. t. J = 2.0 Hz). Ngoii ra. tin hi§u mpt proton olefin dm?c x i c djnh tai 5„6.93 ( I H . s) gpi y (1) cb cau tnjc khung 2-phenyll)enzofuran.
Bing 1: DO lifu phS NMR cua AR3 (1)
c
2 3 4 4a 5 6 7 7a 1' 2' 3' f S
e V
156.6 102.6 122.4 123.5 1137 1577 96.9 157.3 1342 1044 1604 103,9 1604 1044
«,"
156.16 1 0 2 3 121,98 123,07 113,26 157^6 98,49 156,84 134,00 103,97 159,95 103,55 159,95 103,97
DEPT C CH CH C CH C CH C C CH C CH C CH
S," Huh (J" Hi)
6,93(5) 7,37(48,0) 6,76 (dd, 8,0; 2,0)
6,92 (d, 2.0)
6.78 (d. 2,0) 6^6(12.0)
6,78 (d, 2,0)
• db tong CD,Oa' 125 MHz, • 500 MHz Pho "C-NMR vi DEPT cia hpp chat (1) xuat hipn eac tin hi§u cua 14 carton trong d i c6 7 nh6m methin v i 7 carbon b|c 4. Tin hi^u cua vong phenyl ed true doi xumg tai 6,, 134,00 (C-1 *); 103.97 (2C, C-2'/C-61; 159.95 (2C. C-3VC-5') va 103.55 (0-4*); tin hipu vong benzofuran xuat hipn tai 6 j 156.16 (C-2); 102.20 (C-3); 121.98 (C-4); 123.07
(C-4a); 113.26 (C-5); 157.26 (C-6); 98.49 (C-7) v i 156.84 (C-7a).
Cdng thi>c p h i n ti> eua AR3 duprc d^r d o i n l i C „ H O, (M = 242) di.ra v i o c i c k i t q u i ph6 NMR v i pho ESI-MS tpi m * 243,0 [M+H]*.
TO nh&ng du* kipn pho tren, eung s y so s i n h vol hop chat moracin M i^ n i u ra trong b i n g 1 cho phep x i c dinh hop chat (1) t i moradn M. co c i u tnic n h y hinh 1.
H i n h l : C^ulnJchdahpccua4R3(1) HvpchitAR4(2)
(2) l i bOt kfe tinh m i u v i n g , t . , = 255-257'C.
tan it trong nud'c lanh. tan trong methanol, ethanol. ethyl acetat.
Vet c h i m (2) tren SKLM c h u y i n sang m i u v i n g khi h o ndng v i si> dyng t h u i e hi§n m i u la acid sulfuric 10% eho p h i p d y d o i n c h i t (2) l i mOl hop c h i t flavonoid.
P h i 'H-NMR cua h o p c h i t (2) x u i t hi$n tin hi^u d^e tryng ci]a 2 proton t h o m t y o n g t i c 6 vj tri meta v « nhau t j i 6„ 5.91 ( I H . br-s). 5.88 (IH, br-s) v i tin hi§u cua 2 proton d i i xCpng tpi 5„ 6,56 (2H. br-s); n g o i i ra trSn p h i 'H-NMR ciia c h i t (2) x u i t hi§n 2 tin hi§u doublet ciJa 2 proton thom tai 5„ 4.85 ( I H , d, J =11,5 Hz) v i 4,47 ( I H , d. J
=11.5 Hz).
P h i "C-NMR cOa h o p c h i t (2) x u i t hi^n c i c tin hipu cua 13 carbon tpi 8^ 85.24 (C-2); 73,67 (C-3); 197.94 (C-4); 164.41 (C-5); 96.61 (C-6);
168,69 (C-7); 97.60 (C-8); 165.27 (C-9); 101.63 (C-10); 129.18 ( C - r ) ; 108.04 (C-2', 6'); 146.88 (C-3', 5') v i 134,89 (C-4'). Trong 66 tin hi#u CH tpi 6j. 108.04 c6 c y d n g dO cao. phu hop vdi tin hi§u proton c h i p nhau tpi 6„ 6.56. K i t q u i n i y khing dinh vdng B doi xung v i ehi cdn hai carbon CH doi x i m g vd'i nhau. Hai tin hi$u CH tpi 5^ 85.24 vS 73.67 t y o n g i m g vd'i C-2 v i C-3 ciJa vdng C. Sy hi#n di#n cOa nhdm OH tpi C-3 l i m cho g i i tri dO djch c h u y i n hda hoe eua carbon carbonyl C-4 l i n eao hon {5^ 197.94) so vdi c i c tru&ng hop cd n i t ddi C-2/C-3 ( g i i tri 6 chi k h o i n g 1 7 8 - 1 8 0 ppm).
C i e tin hipu NMR edn Ipi cOng cho t h i y v i n g A cd hai nhdm OH tpi C-5 v i C-7. k h i i n cho hai proton H-6 v i H-8 n i m d vj tri octo vdi nhau v i
34 Tli.P C H i DlTCJC HQC - 10/2016 (S6 486 N A M 56)
• Nghien CLFU - Ky thuat
xuSt hi$n d u d i d^ng hai tin hi#u singlel c6 dinh hai tu (broad-stnglet, br-s).
B a n g 2 : Difli$u pho NMR cua AR4 (2)
c
2 3 4 5 6 7 8 9 10
r
2', 6' 3 . 5 ' 4
•S^m 83,97 72,34 198,53 163,34 95,72 167,62 96,73 164,16 101^2 127,91 107,71 146,50 13424
5."
85,24 73,67 197.94 164.41 96.61 168.69 97,60 165,27 101,53 129.18 108.04 146,88 134,89
DEPT CH CH C
c
CH C CH
C
c c
CH C C
S,-'(J,Hzl 4.85 (IH.d. 11.5) 4.47 (IH.d. 11.5)
5.91 (IH. br-s)
5.88(1H.ljr-s)
6.56(2H.bf-s)
• db (rwig CDjOD." (to (91125 MHz, = «J (9/500 MHz T d nhdng phSn tich tt6n eung vi^c so sSnh d d ki^n phd eua (2) vdi d u ki$n ph6 tham khao luang dng PI vS s y xu^t hl§n pic ion phSn t u tt^n pho ESI- MS l$i m/2 319 [M-H]cho ph6p xfic djnh hyp ch^t (2) lei dihydromyricetin.
OH ,0H
OH O
Hinh 2: Ciu triic hda hpc cua AR4 (2) HgpchitARS(3)
(3) 1^ eh^t dSu mfiu vfing, tan trong elorofomi, methanol-
Ph6 ^H-NMR cua (3) xu^t hi§n tin hl^u proton ciia ba nhdm methyl b$c 4 tgi 5„ 1,04 (3H, s); 1,06 (3H, s) v^ 1,94 (3H, d, J = 1,0 Hz); tin hi$u 3 proton olefin t^l 8„ 5,90 ( I H , s); 5,92 ( I H , d, J = 16,0 Hz) vd 5,81 ( I H , dd, J = 5,5 v^ 16,0 Hz) trang do c6 m$t n6i ddi nOi vdng vd m$t noi do! ngogi vdng c6 du hinh trans.
Phan tich trfin ph6 "C-NMR vd D E P r c h o ttiay trong phSn t d hyp ch^t (3) c613 nguyfin t u carton bao g6m: ba nguy&n t u carbon b$e 1, hai nguyen
t u cartjon bac 2, b6n nguyen t u cartxjn b$c 3 va b6n nguyen t u cart>on b|ic 4. T u cfic d d kl$n b^n eho ttiay cau fruc cua hap ch^l (3) UiuOe dgng khung megastigman. S y xu^t hifin eua mOt tin higu praton olefinic dang singlet tgt 5„ 5,90, m^t tin higu carbon cua nhdm carbonyl tgi S^, 201,25 va myt tin hieu cartxin olefinic b§e 4 tai viing trudng ttiap Sj. 167,30 chdng to hop ch^t (3) c6 ehda nhom a.p-eeton khong no. S y co m#l cua mpt lifin ket doi khae vdi cau hinh trans ttong h y p ehiit (3) eung d u y c k h i n g djnh b i n g hai tin hi^u praton olefinic tgi 5„ 5,92 va 5,81 vdi h i n g s6 tuang tae J = 16,0 Hz.
B d n g 3 : D d lipu phi NMR cua AR5 C ' « , "
1 42,3 2 50,6 3 2007 4 126,9 5 166,9 6 80,0 7 132,2 8 132,1 9 73,5 10 67,1 11 234 12 24,5 13 19,6
*e"
42,38 50,72 201,25 12716 167,30 80,14 132,54 132,44 73,63 6728 23,43 24,50 19,57
DEPT C CH,
C CH
C C CH CH CH CH, CH, CH,
»<,
5„«fmu/t..
J'Hz)
2.53 (d, 17,0) 2,18 (d, 17,0)
5,90 (s)
5,92 (d, 16,0) 5,81 (dd. 5,5; 16,0)
4,22 (cHike, 5,5) 3,53 (dd, 5,5; 11,0) 3,48 (dd, 7,0; 11,0)
1,06 (s) 1,04 (s) 1,94 (d, 1,0)
HIIBC(H-^)
1,3, 46,11,12
2,6,13
5.6,
« 9 6,79 7.8.10
6.9 1,2,6.12 1.2.6,11 45,6
• db tong CDpD: • (25 MHz; • 500 MHz P h i HMBC tuong t i e g i t e H-13 (5„ 1.94) vdi C-4 (S^ 127.16)/C-5 (Sj 167.30)/C-6 (5^ 80.14);
giOa H-11 (8„ 1.06) vdi C-1 (5^ 42.38)/C-2 (6^
60.72)/C-6 {6580.14) v i gi&a H.4 (6„ 5.90) vdi C-2 (5c50.72)/C-6 (6^80.14)/C-13 (6^19.57) cho p h i p x i c djnh c6 2 nhdm C H , l i i n k i t vdi C-1 v i mOt noi ddi giOa C-4/C-5. Nhdm carbonyl d y p c x i c djnh tpi C-3 dya v i o tyong t i c HMBC glOa proton H-2 (5„
2.18/2.53) vdi C-1 (S^. 42.38)/C-3 (8^ 201.25)/C-4 (5^ 127.16)/C-6 (5^80.14). Tuong t i c HMBC giO'a H-7 (6„ 5.92) vdi C-5 (5^ 167.30)70-6 (5;.80.14)/C- 8 (5^ 132,44)/C-9 (6^ 73.63) v i giUa H-8 (8„ 5.81) vdi C-6 (6^ 80.14)/C-7 (5^ 132.54)/C-9 (5^ 73.63) cho t h i y ton tai mdt n i i ddi tai C-7/C-8. Ba nhdm hydroxyl tpi vi tri C-6. C-9, v i C-10 cDng d y p e k h i n g dinh b i n g dp c h u y i n dich hda hpc cua e i c TAP CHi DircjC HQC - 10/2016 (S6 486 NAM 56)
Nghien CIPU - K y thuat
nguyen t u carbon nay tten pho "C-NMR (5^ 80,14 (C-6); 73,63 (C-9): 67,28 (C-10)). Cau hinh tuong doi eua nhom hydroxyl tgi C-6 va C-9 d u y c xae djnh la a. a d y a ttfin s u giong nhau ve do chuyen dieh hoa hpe khi so sanh vdi tai lieu da d u y c cong bo cua h y p c h i t oicumegastigman 1 ^ .
T u nhung phan tieh fi^n cung vdi v i ^ so sanh d u kipn pho eua (3) vdi d u kien pho ttiam khao tucmg dng f^> neu ra frong bang 3 va s u x u l t hifin pic ion phan t u tten pho ESI-MS tgi m/^ 241 [M+H]- cho phfip xae djnh h y p chat (3) d u y c xae dinh la cucumegastigman I c6 cong ttidc phan t d la C^jHjoO, (M = 240).
12 O H
Hinh 3: Cau tnic hda hpc cue AR5 (3) Ket luan
T u lfi sa ke, da phfin l$p d u y c 3 hyp chat: AR3 (1) lfi moracin M, AR4 (2) lfi dihydromyricetin vfi AR5 (3) lfi cucumegastigman I. Cau ttdc cua cac hyp e h i t phfin l$p d u y c xfic dinh d y a tren d u ligu ph6 khoi luyng (ESI-MS), ph6 cpng h u d n g t u hgi nhfin (NMR) vfi so sfinh v d i tai ligu Iham khfio. L l n d i u tifin 3 hyp c h i t nfiy d u y c phfin lgp t u lfi sa kfi.
T a i li^u t h a m k h a o
1. V6 Vfin Chi (2004), 7i> diin thpc v$l Ihdng dpng.
Nhfi xuat ban Khoa hpc vfi ky ttiugt, tfip 1, 36&-370.
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{Ngdy nh^n bdi: 16/12/2015 - Ngdy duypt dang: 03/10/2016)
Nghien cihi dieu che kem pyrithion
Trirorng Phirong*, Tr5n Thi Thao NguySn
Dgi hpc Y- Dupc TP. Hd Chi Minh 'E-mail: [email protected], vn S u m m a r y
Zinc pyrithione, as a coordination complex of zinc for use in treatment of dandruff and seborrhoeic dermatitis, was prepared from bromopyridine. zinc sulfate, peracatic acid.... The preparation was optimized by software MODDE 5.0. for stability, simplicity and being suitable for Vietnam conditions.
The purity of the obtained zinc pyrithione was controlled by thin layer chmmatography (TLC), and the stmcture was detennined by elemental analysis and IR, 1 H-NMR, MS.
Keywords: Zinc pyrithione. bromopyridine, zinc sulfate, peracetic acid, dandruff, seborrhoeic dermatitis.
TAP C H i DU'OC HQC - 10/2016 (S6 486 NA.M 56)
