• N g h i e n C L F U - K y t h u a t S u m m a r y
As the model of mice bearing human cancer tumor is very necessary for researching efficacy of anticancer drugs, first time in Vietnam, a model of T-cell deficient mice bearing human prostate cancer tumor established for the first time in
Vietnam. In this way, mice were injected with antinuclear antibody conjugating radioactive iodine and concentration of antinuclear antibody conjugating radioactive iodine in tumor was estimated. The findings proved the antinuclear antibody conjugating radioactive iodine appeared in the tumor just only one hour after the injection and remained there up to 72th hour.
Tai lieu t h a m k h a o
1. Sharkey, F.E., Fogh, J. Consideration on the use of nude mice for cancer research. Cancer Metastasis Rev.,(1984) 3: 341-360.
2. Qiang Zuo, Amin Li. Biodistribution and Anti- tumor Activities of the 131l-labeled Rituximab in Nude Mice Bearing Human Burkitt's lymphoma. Clin Oncol Cancer Res (2009) 6: 256-262
3. Kiriazis, A. P., McCombs, W.B. Biological behavior of human malignant tumor grown in the
nude mouse. Cancer Res., (1981) 41: 3995-4000.
4. Povlsen, CO., Regaard, J., Fogh, J. Long-term growth of human tumor in nude mice : Evatuation of stabiulitry. In: J. Fogh and B.C.Giovannella, The nude mouse in Experimental and Clinical Reseach . Vol. 2, pp. 97-93. New York: Academic Press, (1982).
5. Kjonniksen, I. Novel human tumor metastasis model in immunodeficient mice and and rat- development, characterization, and examples of practical application. Thesis, Universitry of Oslo, 1992.
6. Tonkin K, Tritchler D, Tannock I. Criteria of tumor response used in clinical trials of chemotherapy. Journal Clinical Oncol (1985) 3: 870-5 7. Moskowitz CS, Jia X . S simulation study to evaluate the impact of the number of lesions measured on response assessment. Eur Journal Cancer (2009); 45: 300-10
8. Mireal F.Leahy, John F. Seymour. Multicenter Phase II Clinical study of Iodine - 131- Rituximab Radioimmunotherapy in Relapsed or Refractory Indilen Non-Hodgkin's Lymphoma. Journal of Clinical Oncology. (2006), Vol 24, Num 27, Sept.
9. Inge Kjonniksen, Mette Winderen. Validity and Usefulness of Human Tumor Models Established by Intratibial Cell Inoculation in Nude Rats. Cancer Reseach (1994), 54,1715-1719, April 1.
N g h i e n c u u s a n g l o c t a c d u n g t r e n c o * t r o n v a
^ r r
d o n g c a m m a u c u a flavonoid c h i e t x u a t t i r c a y
diep ca (Houttuynia cordata Thunb.) Viet Nam Nguyen Thj Ngoc Tram1, Vu Thi Ngoc Thanh3
Tran H u n g2, Dao Van Phan3 Cong ty CP DL TW21, Dai hpc Y Duac TP. Ho Chi Minh2,
Truang dai hoc Y Ha Ngi3 Dat vdn de
O Vidt Nam theo tac gia Nguydn Dinh Hoi Nguydn Manh Nhdm 1 2 1 qua dieu tra khao sdt cho thiiy ty Id ngudi miic bdnh trT tu 35% ddn 40%.
Dd cd thudc chu-a trj bdnh trT, Vidt Nam dd nhdp cdc san pham thudc nhu Ginkor Id san phim bdo chi tu hoat chit flavonoid dugc chiit xuit tu Id Ginkgo biloba, ngodi ra cdn nhdp mdt sd san phim khdc dugc san xuit tu cdc nguydn lidu tdng hgp hda hpc nhu thudc dan Preparation H*
kem bdi tryc trdng Proctolog, vidn tpa dugc Mastu " S forte. Trong nhCrng ndm qua d i cd thudc diiu tri bdnh tu cdy diip cd cd nhiiu tdc gia trong nude | 6' dd nghidn cuu chiit xuit flavoniod chu yiu gdm Phloretin-2'-0-B-D- glucopyranosid, quercetin-3-CMs-D-galactopyranosid, quercetin-3-O-a-L-arabino-furanosid, isoquercitrin cd tdc dyng hgn chi sg peroxy hda lipid mdng t i bdo gan khd tdt vd tdc gia171 cQng dd nghidn cuu cdc flavonoid chiit xuit tu diep cd cd hoat
TAP CHI DUOC HOC-4/2011 (SO 420 NAM 51) 19
• N g h i e n c u » u - K y t h u a t tinh gay doc te bao vdi ddng te bao S-180. Do dd d cdng trinh nay chung tdi da tien hanh nghien cuu tim kiem cac hoat chat sinh hpc chua tri bdnh trT dya trdn kinh nghidm ddn gian, nhan dan ta thudng su dung cdy diep cd (Houttuynia cordata Thunb.)'3,41 de chua bdnh trT. Chung tdi mong muon tao ra dugc mdt logi thuoc mdi dieu trj trT dugc bdo chd tu cdc flavonoid chiet xudt tu Id cdy diep cd Vidt Nam.
De thyc hidn dugc muc tidu tren, chung tdi dd tidn hdnh chiet xudt cao chiet gidu flavonoid vd nghidn cuu sdng Ipc tdc dung dugc ly trdn ca tran vd ddng cam mdu.
N g u y e n lieu va p h i n y n g p h a p n g h i e n cu>u
Nguydn lieu La cay diep ca
Ld cay diep cd duac thu hdi vdo thdng 07/2009 tai Long An. Ld duac rua sach, xu ly vd siy khd d nhidt dp 60°C - 70°C.
Ld diep cd duac che thdnh cao chiet gidu flavonoid: DLDC-N-TH vd DLDC-E-TH. Hai cao chiet ndy duac dung Idm nguydn lidu thu nghidm.
Ddng vat thi nghiem
Chudt nhdt trdng chung Swiss, ca hai gidng, khde manh, trpng lugng 20,0 ± 2,0 gam do Vidn Vd sinh Djch td Trung uang cung cap.
Thd chung Newzeland White, ca hai gidng, khde manh, Idng tring, trpng luang 1,8 - 2,5 kg do Trung tdm chdn nudi Vidn kiim nghidm Trung uang cung cip.
Ddng vdt duac nudi trong phdng thi nghidm cua Bd mdn Duac ly - Trudng Oai hpc Y Hd Ndi tu 3-5 ngdy trudc khi tiin hdnh nghidn cuu bdng thuc dn chuin ddnh ridng cho loai ddng vdt Vidn Vd sinh djch td trung uang cung cip, udng nude tu do.
Phirang phap nghien cum Chiet cao chiet giau flavonoid
Chiit cao chiit giau flavonoid bing dung mdi node
Duac lidu (Id diep cd khd) dugc xay den dang bdt thd vd cho vdo binh inox rdi thdm vdo binh chiit 15 the tich nude, dun ndng vd gitr hdn hgp d gin nhidt dd sdi cua nude trong 2 gid, thinh thoang khuiy deu, sau dd gan liy djch chiit. Thdm vdo bd duac lieu 8 the tich nude vd chiit tiip cho tdi khi djch chiit cho mdu rit nhat vdi FeCI3. G0p chung cdc djch chiet, Ipc
qua bdng. Loai tap vd cd trdn bep cdch thuy tdi dang cao ddc, thu dugc cao chiit nude cua diep cd, ky hidu DLDC-N-TH.
Chiit cao chiit giiu flavonoid bing dung mdi cdn
Dugc lidu (Id diip cd) dugc xay den dgng bdt thd, dugc Idm am bdng cdn 95%, rdi cho vdo binh ngam kidt. Nap cdn 95% vdo binh ngam kidt, vd ngdm trong 24 gid. Sau dd, nit djch chiit cho den khi djch chiet cdn chi cdn phan ung rit yeu vdi FeCI3. G0P cdc djch chiit, loai tap vd cd thu hdi dung mdi tdi dang cao ddc, thu dugc cao chiet cdn diep cd, ky hidu DLDC-E-TH.
Djnh tinh flavonoid trong cao chiet Flavonoid trong cdc cao chiet dugc djnh tinh bdng phan ung cyanidin vdi Mg/HCI 1 5 1 vd phuang phdp sdc ky Idp mdng vdi thudc hidn mdu Id FeCI3/EtOH.
Phirong phip nghien cim tic dung diroc ly Nghiin ciru sing loc tic dung trin co tron - Tic dung cue DLDC-N-TH vd DLDC-E-TH trdn ca tran thdnh mach tai thd cd Idp.
Tai thd dugc cd Idp theo phuang phdp Kravkov. Truyen dung djch Ringer khdng pha hodc cd pha DLDC-N-TH vd DLDC-E-TH theo 3 ndng dd khdc nhau d dd cao 100 cm vdo ddng mach gifta tai thd.
Cdc ndng dd miu nghidn cuu:
- Lieu 1: Ndng dd 1,8%. Ddy Id ndng dd trong mdu thd udc lugng cd tdc dyng tuang duang vdi ndng dp trong mdu ngudi d liiu udng 30g duac lidu/ngudi/ngdy (gid su thudc hip thu hodn todn).
- Lieu 2: ndng dd 0,9% (bdng 1/2 liiu 1).
- Lieu 3: ndng dd 0,45% (bdng 1/4 liiu 1).
Dim sd gipt dung djch chay ra d 2 tTnh mach ria tai thd trong thdi gian 1 phut trudc vd sau khi dung miu nghidn cuu. Neu sd gipt chay ra d 2 tTnh mach ria tai tdng, chung td mach gidn, ho$c nguac lai, neu sd gipt giam chung td mach co.
- Tdc dung cua DLDC-N-TH vd DLDC-E-TH trdn ca tran thdnh rudt cd Idp.
Rudt thd dugc cd Idp theo phuang phdp Magnus, nudi trong dung djch Tyrod.
Ghi nhu ddng rudt binh thudng vd nhu ddng rudt thd sau khi pha cao DLDC-N-TH ho$c DLDC-E-TH vdo dung dich Tyrod vdi 3 ndng dd khdc nhau (nhu nghidn cuu trdn tai thd cd Idp).
20 TAP CHI DUOC HOC - 4/2011 tt!<S 4.7.0- M A M sn
• N g h i e n c t j r u - K y t h u a t Nghiin cCru sing loc tic dyng ddng cim miu - Tac dung cua DLDC-N-TH va DLDC-E-TH len thai gian chay mau va thdi gian ddng mau cua chudt.
Chudt nhit tring duac chia ngau nhidn thdnh 4 Id, moi Id 10 con:
Ld 1: uong DLDC-N-TH lidu 7,2g dugc lidu/kg/ngdy; (lieu cd tdc dung tuang duang trdn ngudi, tinh theo hd so 12)
Ld 2: uong DLDC-N-TH lieu 14,4g duac lidu/kg/ngdy (gap 2 lan lidu Id 1);
Ld 3: uong DLDC-E-TH lieu 7,2g duac lidu/kg/ngdy; (lidu cd tdc dung tuang duang trdn ngudi, tinh theo hd so 12)
Ld 4: uong DLDC-E-TH lieu 14,4g duac lidu/kg/ngdy (gap 2 lan lieu Id 3).
Do thdi gian chay mdu vd ddng mdu binh thudng cua chudt trudc khi cho udng cdc miu nghidn cuu bing cdch cdt dudi chudt mdt doan ddi khoang 1mm, nhd 2 gipt mdu vdo lam kinh de tinh thdi gian ddng mdu. Sau dd nhung dudi chudt vdo nude i m 37°C. Theo ddi thdi gian tu khi cit dudi chudt tdi khi mdu d dudi chudt ngung chay (thdi gian chay mdu). Sau dd cho chudt udng mdu nghidn ciru trong 7 ngdy lien.
Ngdy cudi ciing, sau udng DLDC-N-TH vd DLDC-E-TH 1 gid, do lai thdi gian mdu chay vd mdu ddng nhu trdn. So sdnh thdi gian chay mdu vd ddng mdu trudc vd sau khi dung mau nghidn ciru bing test trudc - sau.
- Tdc dgng cua DLDC-N-TH vd DLDC-E-TH ldn sd luang tiiu ciu trong mdu chudt
Chudt nhdt trdng duac chia ngau nhidn thdnh 6 Id, mdi Id 10 con:
Ld 1: udng nude cit 0,2 ml/10g/ngdy;
Ld 2: udng DLDC-N-TH lieu 7,2g duac lidu/kg/ngdy;
Ld 3: udng DLDC-N-TH lieu 14,4g duac lidu/kg/ngdy;
Ld 4: udng dung dich CMC 0,5% - 0,2ml/10g/ngdy (dung mdi pha mdu DLDC-E-TH);
Bang 1: Tic dung cua DLDC-N-TH trin co tron
Ld 5: udng DLDC-E-TH lieu 7,2g duac lidu/kg/ngdy;
Ld 6: udng DLDC-E-TH liiu 14,4g duac lidu/kg/ngdy.
Cho chudt udng cdc miu nghidn ciru vd dung mdi (nude hodc CMC 0,5 %) trong 7 ngdy liin. Ngdy cudi ciing, sau udng mdu nghidn ciru hodc dung mdi 1 gid, liy mdu chudt dim sd luang tiiu ciu bdng dung djch ABX Minidil LMG cua hdng ABX - Diagnostics trdn mdy Vet abc™
Animal Blood Counter.
- Tdc dyng ciia DLDC-N-TH vd DLDC-E-TH den ty Id prothrombin trong mdu thd
Thd dugc chia thdnh 4 Id, mdi Id 5 con:
Ld 1: udng nude cit 2 ml/kg/ngdy;
Ld 2: udng DLDC-N-TH lieu 1,8g duac lidu/kg/ngdy; (lieu cd tdc dyng tuang duang trdn ngudi, tinh theo hd sd 3);
Ld 3: udng CMC 0,5% - 2 ml/kg/ngdy (dung mdi pha mdu DLDC-E-TH);
Ld 4: udng DLDC-E-TH liiu 1,8g duac lidu/kg/ngdy; (lieu cd tdc dyng tuang duang trdn ngudi, tinh theo hd sd 3)
Thd duac udng miu nghidn ciru vd dung mdi (nude hodc CMC 0,5%) trong 7 ngdy liin.
Ngdy thir 7, sau udng 2 gid, liy mdu thd do thdi gian Quick vd tinh ty Id prothrombin.
Thdi gian Quick vd ty Id prothrombin duac do bdng mdy CA 1500 cua hdng Sysmex (Nhdt
Ban) tai khoa Huyit hpc bdnh vidn Bach Mai.
K i t qua va ban luan Kit qud chiit xuit
Chiet bdng dung mdi nude: tu 2,0 kg diip cd thu duac 400 g cao flavonoid todn phin.
Chiit bdng dung mdi cdn: tir 9,0 kg diip cd thu duac 500 g cao flavonoid todn phin.
Sang loc tac dung tren ca tran
Tic dung cua DLDC-N-TH vi DLDC-E-TH tren co tron thanh mach tai thd cd lap
Kit qua khao sdt d bang 1 vd 2.
thinh mach tai thd cd lip
Thuoc n So gigt/phiit So v&i Ringer
Thuoc n
(X±SE) % thay doi p (Student test)
Ringer 8 44,6 ± 2,7
Ndng dO 0,45% 8 58,9 ± 2,7 Tang 32,9 ± 3,6 < 0,01
Ringer 8 44,5 ± 3,1
Ndng 66 0,9% 8 46,5 ± 2,8 Tdng 5,1 ±1,8 >0,05
Ringer 8 49,5 ± 3,1
NdngdO 1,8% 8 40,8 ± 2,5 Giam 17,5 ±2,4 <0,05
TAP CHl DU'QC HQC - 4/2011 (S6 420 NAM 51) 21
N g h i e n e l m - K y t h u a t - Miu DLDC-N-TH a nong dp 0,45% cd tac dung lam gian mach rd rdt, lam tdng so gipt chay ra tu tTnh mach ria tai thd 32,9%. 6" nong dp 0,9%, so giot chay ra tu tTnh mach ria tai thd khdng cd sy khac bidt so vdi khi truyen dung dich Ringer khdng cd mau DLDC-N-TH .
- CJ nong dd 1,8%, DLDC-N-TH cd tac dung ngugc lai, Idm co mach rd ret, Idm giam 17,5%
so gipt chay ra tir tTnh mach ria tai thd.
Bang 2: Tdc dung cua DLDC-E-TH tren co tron thanh mach tai thd co lap
sd So v&i Ringer Thuoc n giot/phut % p (Student
CX + SE) thay doi test) Ringer 8 47,1 ±1,5
N6ng dp
0,45% 8 96,4 ± 2,9 Tang
104,8 ±4,1 < 0,001 Ringer 8 38,9 ±2,6
Nong dp
0,9% 8 98,3 ±8,0 Tang
152,0 ±7,6 < 0,001 Ringer 8 43,9 ±4,1
Nong dp
1,8% 8 116,5±8,6 Tang
169,9 ± 11,8 < 0,001 - Mdu nghidn cuu DLDC-E-TH khdng tan hoan todn trong dung dich Ringer
- 6 ca 3 ndng dd 0,45%, 0,9% vd 1,8%, mdu DLDC-E-TH deu cd tdc dung Idm gidn mach rd ret, lam tdng sd gipt chay ra ty tTnh mach ria tai thd ty 104,8 % den 169,9 %, tdc dung gidn mach tl Id
Bang 3: Tdc dung cua DLDC-N-TH va DLDC-E-TH ldn thOi gian chiy miu
thudn vdi ndng dd DLDC-E-TH dd dung.
Tac dung cua DLDC-N-TH va DLDC-E-TH tren co tron thanh rugt cd lap
Tic dung cua DLDC-N-TH •
- Ndng dd 0,45%: truang lyc co thdnh rudt tdng manh, sau dd truang lyc giam din nhimg vdn giCr d mftc cao. Nhu d0ng rudt tdng bidn 6b nhirng giam tan sd.
- Ndng dp 0,9% vd 1,8%: trirong lyc co thdnh rudt tdng manh, sau dd truang lyc gidm din nhirng vdn giir d mire cao. Nhu ddng rudt giam ca tan sd vd bidn dp.
Tic dung cua DLDC-E-TH
- Mdu nghidn ciru DLDC-E-TH khdng tan hodn todn trong dung djch Tyrod.
- Ndng dd 0,45%: trirong lyc co thdnh rudt giam nhe, ngay sau dd trd v i binh thirdng. Nhu ddng rudt giam nhe t i n sd, bidn dd it thay ddi.
- Ndng dd 0,9%: trirong lyc co thdnh rudt hiu nhir khdng thay ddi. Nhu ddng rudt giam nhe tin sd, bidn dd it thay ddi.
- Ndng dp 1,8%: trirong lyc co thdnh rudt hiu nhir khdng thay ddi. Nhu ddng rudt giam tin sd nhirng tdng bidn dp.
Sang loc tac dung ddng - cdm mdu
Tac dung cua DLDC-N-TH vi DLDC-E-TH len th&i gian chay mau vi thoi gian ddng mau ciia chuot.
Kit qua khao sdt d bang 3 vd 4:
Lo nghien ciru Th&i gian chay mdu (giay) (X + SE) P Lo nghien ciru
Trw&c Sau (trw&c-sau)
DLDC-N-TH 7,2g DL/kg 10 79,4 ± 15,2 124,1± 18,7 (tang 56,3%) <0,05 DLDC-N-TH 14,4g DL/kg 10 79,9 ± 5,0 151,1 ±8,9 (tdng 89,1%) < 0,001
DLDC-E-TH 7,2g DL/kg 10 88,9 ± 12,4 56,1 ± 7,3 (giam 33,5%) <0,01 DLDC-E-TH 14,4g DL/kg 10 76,9 ±8,2 44,2 ± 2,6 (giam 42,5%) <0,01 - Mdu DLDC-N-TH vdi ca hai lieu 7,2g dirge
lidu/kg/ngdy vd 14,4g dirge lidu/kg/ngdy udng trong 7 ngdy Idm kdo ddi rd rdt thdi gian chay mdu trdn chudt tu 56,3 - 89,1%, tdc dung ti Id thuan vdi lieu diing.
- Mdu DLDC-E-TH vdi ca hai lieu 7,2g dugc lidu/kg/ngdy vd 14,4g dugc lidu/kg/ngdy udng trong 7 ngdy Idm giam rd rdt thdi gian chay mdu trdn chudt tu 33,5 - 42,5%, tdc dyng ti Id thudn vdi lieu dung.
Bang 4: Tic dung cua DLDC-N-TH vi DLDC-E-TH lin fhd; gian ddng miu
Lo nghien ciru n Th&i gian dong miu (giay) (X ±SE) . p (trw&c-sau) Lo nghien ciru n
Trw&c Sau . p (trw&c-sau) DLDC-N-TH 7,2g DL/kg 10 195,2 ±25,3 301,2± 38,9 (tdng 54,3%) <0,05 DLDC-N-TH 14,4g DL/kg 10 151,0 ±17,2 224,9 ±14,5 (tang 48,9%) <0,01
DLDC-E-TH 7,2g DL/kg 10 197,6 ± 25,6 184,6 ±26,6 (giam 6,6%) >0,05 DLDC-E-TH 14,4g DL/kg 10 174,8 ± 19,2 114,8 ±7,9 (giam 54,3%) <0,05
22 TAP CHI Dl/QC HQC - 4/2011 (SO 420 NAM 51)
• N g h i e n c u » u - K y t h u a t - Mau thu6c thu DLDC-N-TH vdi lieu 7,2g dugc lidu/kg/ngdy va 14,4g dugc lidu/kg/ngdy u6ng trong 7 ngay lam keo dai rd rdt thdi gian ddng mau trdn chudt nhlt tring tu 48,9 - 54,3%.
- Miu thuoc thu- DLDC-E-TH vdi lieu 14,4g dugc lidu/kg/ngdy udng trong 7 ngdy lam giam
Bang 5: Tdc dung cua DLDC-N-TH len sd Iwong
rd rdt (54,3%) thdi gian ddng mdu trdn chudt.
Liiu 7,2g duac lidu/kg/ngdy cd xu hudng Idm gidm thdi gian ddng mdu cua chudt, nhung su khdc bidt chua cd y nghTa thong kd.
Tac dung cua DLDC-N-TH va DLDC-E-TH len so Iwong tieu cau trong mau chuot
Kit qua khao sdt ghi d bang 5 vd 6.
tiiu ciu trong miu chudt Ld nghien ciru n So Iwong tieu ciiu (don vi/mm3)
(X + SE)
(Student test) P
Chung 10 601,5 + 63,3
DLDC-N-TH 7,2g dupe lieu/kg 10 889,3 + 43,1 (tang 47,8%) <0,01 DLDC-N-TH 14,4g dugc lieu/kg 10 805,6 ± 72,5 (tang 33,9%) <0,05 Mdu DLDC-N-TH ca hai lieu 7,2g duac
lidu/kg/ngdy vd 14,4g duac lidu/kg/ngdy, udng trong 7 ngdy diu Idm tdng rd rdt so luang tieu ciu trong mdu chudt 33,9% - 47,8%.
Bang 6: Tic dung cua DLDC-E-TH len sd Iwong tiiu ciu trong miu chuQt
Lo
nghien ciru n
So Iwong tieu cau (don vj/mm3)
(X±SE)
(Student P test) Chung
(CMC 0,5%) 10 596,4 ± 60,7
DLDC-E-TH 10 810,5 ±50,2 <0,05 7,2g dugc lidu/kg 10 (tdng 35,9%) <0,05
DLDC-E-TH
14,4g dugc li$u/kg 10 918,0 ±57,6
(tang 53,9%) <0,01 Miu DLDC-E-TH ca hai lieu 7,2g duac lidu/kg/ngdy vd 14,4g duac lidu/kg/ngdy, uong trong 7 ngdy deu Idm tdng rd rdt so luang tieu ciu trong mdu chudt tu 35,9% - 53,9%, tdc dyng ti Id thudn vdi lieu dung.
Tic dyng cua DLDC-N-TH vi DLDC-E-TH den ty li prothrombin trong miu tho.
Kit qua khao sdt ghi d bang 7.
Bang 7: Anh hwdng cua DLDC-N-TH vi DLDC-E-TH din ty li prothrombin trong miu thd
L6
nghiin ciru n Ty /? prothrombin (%)(X±SE)
P (Student
test) Chung
(nude cit) 5 100,0 ±8,8 DLDC-N-TH
1,8g dugc lidu/kg 5 103,5 ±3,0 >0,05 Chung
(CMC 0,5%) 5 100,0 ±4,9 DLDC-E-TH
1,8g dugc li$u/kg 5 100,6 ±4,2 >0,05
Hai miu nghidn cCru DLDC-N-TH vd DLDC- E-TH lieu 1,8g duac lidu/kg/ngdy, uong trong 7 ngdy khdng dnh hudng tdi ty Id prothrombin trong mdu thd.
Tidu chuin cua mdt loai thuoc diiu trj bdnh trT tot phai cd cdc tdc dyng sau:
- Trdn rudt: Idm co ca, tdng truang Igc ca thdnh rudt, giam nhu ddng, kdo duac bui trT trd ve.
- Ldm co mach, tdng s6 luang tiiu ciu, rut ngdn thdi gian chay mdu, Idm cim mdu. Kit qua nghidn cuu trdn cho thiy:
- Miu nghidn cuu DLDC-N-TH chiet xuit bdng dung mdi nude cd tdc dung tot Idm co ca rudt manh (tdng truang Igc ca thdnh rudt, giam nhu ddng rudt) vd Idm tdng so luang tiiu ciu nhung lai Idm kdo ddi thdi gian mdu chay vd thdi gian mdu ddng, cd le lidn quan den tdc dung Idm gidn mach cua thuoc thu d cdc ndng dd < 0,9%.
- Miu DLDC-E-TH Id mdu chiet xuit bdng dung mdi cdn cd tdc dung Idm tdng rd rdt sd lugng tiiu ciu, rut ngdn thdi gian mdu chay vd thdi gian mdu ddng khi cho ddng vdt udng.
Ridng tdc dung trdn ca quan cd Idp (tai vd rupt), nhu trdn da phdn tich, khdng phan dnh chinh xdc tdc dung cua thudc vi chua the hidn duac tdc dung cua thdnh phin khdng tan trong nude cd trong mdu thudc thu, ndn kit qua khdng chinh xdc nhu khi cho ddng vdt udng, thudc dugc hip thu qua dudng tidu hda. VI vdy, cd thi thdnh phin khdng tan trong nude cua mau DLDC-E-TH Id thdnh phin chinh cd tdc dung dieu trj bdnh tri.
K§t luan
O i tdi dd nghidn cuu chiit xuit cao chiet gidu flavonoid tu Id cdy diep cd (Houttuynia
TAP CHI DUOC HOC - 4/2011 (S6 420 NAM 51) 23
N g h i e n c u ? u - K y t h u a t cordata Thunb.) Vidt Nam vai hai loai dung mdi nude va dung mdi c6n. Ket qua la dd thu duac hai miu cao giau flavonoid la DLDC-N-TH, DLDC-E- TH. Cac cao chiit nay dd duac nghidn cuu tac dung duac ly trdn ca tran va ddng cim mau. Kit qua khao sat cho thiy cao gidu flavonoid chiet bing dung mdi cdn DLDC-E-TH cd tdc dyng trdn ca tran vd ddng cim mdu tdt han cao chiit bdng dung mdi nude DLDC-N-TH.
Nhu vdy budc diu sdng Ipc chiing tdi thiy nguydn lidu d i san xuit thudc dieu trj trT tif cdy diip cd Vidt Nam se duac chpn Id cao chiit bdng dung mdi cdn vd ddy Id ca sd khoa hpc d i tidp tuc nghidn cuu tdch cac phdn doan tir cao chiit cdn tien tdi nghidn cuu tdc dyng duac ly cua cdc phdn doan flavonoid.
S u m m a r y
The effects of the total flavonoids extracted with ethanol (DLDC-E-TH) and water (DLDC-N- TH) from Vietnamese Houttuynia cordata Thunb. on the smooth muscle contraction and blood coagulation-hemostasis were screened for treatment of hemorrhoid. Experimental studies
were carried out on in situ and isolated organs of mouse and rabbit. The ethanolic extracts were better effective than aqueous extracts on both contraction of smooth muscle and the blood coagulation-hemostasis.
Tai lieu t h a m k h a o
1. Nguyen Dinh Hdi, Didu trj benh trT bing laser C02, Y hoc thuc hanh, (1994), 4, tr.15-19.
2. Nguyen Mgnh Nhdm, Nguyen Dinh Chi, Tinh hinh b#nh trT 0 m0t nhd mdy, Ngoai khoa, Tdng hQi Y Dirov hoc Viit Nam, (1999), 4, tr. 15-21.
3. Dd Tit Lgi, Nhirng cdy thudc va vj thudc Viet Nam, NXB KHKT, (2003), tr.40.
4. V6 Vdn Chi, Tu dien cdy thudc Viet Nam, NXB Y Hoc, Hi NOi, (1997), tr.406.
5. NguySn Vdn Ddn, NguySn Viet Tuu, Phuong phap nghien cuu hda hpc cac cdy thudc, NXB Y hoc, (1985).
6. Hodng Thanh Huang, Tran Quynh Hoa va cpng sy , gop phin nghidn ciru thdnh phin flavonoid chiet xuit tu Id cdy diep cd (Houttuynia cordata Thunb) ciia Vidt Nam, Tap chi Duoc hoc, (2002), 9, tr.13.
7. Hodng Thanh Huong, Hd Viet Bao vd c$ng sir thdm dd kha ndng chdng ung thu cua thdnh phin flavonoid chiit xuit tir Id diip ca (Houttuynia cordata Tnunb) ciia Vidt Nam, Tap chi Duoc hoc, (2003), 10, tr.9.
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D a n h g i a a n h h i r f r n g c u a d i c h c h i e t m e t h a n o l t o a n p h a n v a d i c h c h i e t p h a n d o a n h e x a n
r e c a y c h o c m a u N a m B o ( R a d i x S a l a c i a c o c h i n c h i n e s i s L o u r . ) t r e n s u h a p t h u g l u c i d
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Do Thj Nguyft Que Phi T h j XuyeV, Phvong T h i f n Thiromg2, Nguyen Duy Thuan3, Nguyen Tran T h j Giang Hiroug4 'Dgi hqc Dugc Hd Nqi, , 2 Vien Dugc liiu,
3Hoc vi^n Yhoc cd truyen, 4Dgi hoc Y Hd Nqi Ciing vdi sy gia tdng nhanh chdng cua bdnh
OTO trdn todn cau vd cdc hdu qua n$ng n i do cdn bdnh ndy gdy ndn Id sy cd gdng nd lyc cua cdc nhd khoa hpc nhdm Idm rd nguydn gdy bdnh, tim ra cdc thudc didu trj, phdng ngira...
Hidn dd cd mdt sd thudc diiu trj OTO nhung
24 TAP CHI Dl/OC HOC - 4/2011 rs6 420 NAM SI 1
