KHOA HOC C 6 N C NGHE
PHAM LAP, T U Y i M CHOM VA OAMH GIA KHA M 4M6 HOA TAM PHOT PHO CLIA V I KHLIAM DUPC PHAM LAP
TLT OAT PHEM VLJMB R i LLIA MLIA MOI
L* Ngpc Tlianh Xuta', Le Vinh 11.^0^. NguySn Qu6c Khuong^
T6MTAT
Vi«c bao ton vung liia mua noi o An Giang gin Iitn voi v i ^ duy Ui c«ng dong vi sinh v?t ct vung nay. \luc tiia cua nghiSn ciiu la Uiyia chon nhiing dong vi khuin vung rS lua niiia nii co kha nang hoa t,ui phot pho (P) Ui«n vpng. Mt qua cho thiy t6ng so 53 dong vi khujn viing re dupc phan Up til moi truong Burk's va NBRIP. Trong do. nam dong vn khuan co lutmg P hoa lan cao nhat iii mot micmg Burk s la 22- BD6. 23-BD6. 27-BD6a. 24-BD6c va 10-BD6b vpi luong P hoa tan 14.15 - 16.51 mg P^O, L'. Ngoai ra, kha nang hoa lan P cua hai dong vi khuan 2S-Nn6b va 3-M16 duvc phan lap til moi ffuong NBRIP cao khac biet y nghia Uiong ke so voi cac dong vi khuan c6n lai voi ham lupng P hoa ian 6,33 - 7.13 mg P2O5 L sau 15 ngay li. CSn djnh danh cac dOng vi khuan da tuyOn chpn va ap dgng Uong dieu kien nha luoi hoac dong rupng d^ giam phan P cho cay tr^ng.
Tijr khPa://oa/art/^ lua mua ndi. li khudn iiingr^. dat phen.
1.BATVMBE
Ltia niiia noi co nang suat thap CToure et al., 1987), nhimg co gia tri cao vi giau protein, vitamin E va chat sat sp viji Ida cap san. Ngoai ra, ham lupng dupng chat trong rom cao co the dupc tan dung lam thiic an chp dpng vat (Srivastava et al., 2017). Tuy nhien, day khong phai la nguyen nhan chinh dan d^n liia niiia noi b An Giang dupc bao ton. Liia mua n6i c6 dac di^m phat triSn cimg voi su gia tang ciia mtrc ntn>c. di^u nay phu hpp vtii vi tri thupng nguon DBSCL nhu tinh /Vn Giang. vi vay. vipc duy di dien tich liia miia npi cung gtip phan Iam da dang he sinh thai n6ng nghidp. Hi^n nay. khu bap ton ltia miia npi dupc thanh lap vcri muc tieu duy di 200 hecta p giai doan 2016 - 2020 va len den 500 hecta vao nam 2030 tai xa \inli Phuoc. huy^n Tri Ton Do do. viec bao l6n cong dong vi sinh vat p moi truong nay la can thiet. Dl^u nay cQng ddng nghia vcri cong dong vi smh vat co lpi d-ong mPi truong dat dupc duy tri. Dat trPng liia miia noi cr An Giang chu yen la dat phen nen viec cung cap duong chat P cho cay lua gap nhieu trp ngai. P ton tai trong dat a cac dang mudi hoac cac hpp chat hiru ca. Mac dii cac hpp chiit P nhieu trong dat nong nghiep. hau het chi tdn tai a dang kho hoa tan (Miller et al., 2010). DPI viri cay tring can klioang 30 ymol L' de dat nang suSt t6i
' Tnrong Dai hoc .^n Giang
• Truong Dai hoc Can Tho
hao, nhung lupng P him dung cho cay h-png chi 1 limol L' b nhi6u loai dat. Do do. su khong htru dung Ciia P dirpc xac dinh la y^u to gioi han sinh tnrcmg cay d-ong (Daniels et al., 20O9). Dieu nay cho thay.
can thiet bdn phan P 6 dang hoa tan. nhung di^u nay ciing gap tnr ngai la phan P bon vao hi co dinh rat nhanh vpi dpc chat trong diit. Nghien cim truoc day chp thay. hiOu qua bpn phan P kho vupt qua 30% biri vi su c6 dinh ciia no (Sharma el al, 2013), Ngoai ra.
P cung mill di cr dang hire di va chay han va chi 10 - 20* la hOu dpng cho cay dong (Sashidhar va Podile, 2009). Phan P co nguon gdc tir da phdt phat, day la nguon tai nguyen khong tai tao va nguon du dir toan cau hi^n nay co thi can kiet dong 10 -100 nam tin (Cordell etal, 2009). Vi vay. viec tim kiem nguon P thay thi dong nong nghiep la quan dong. Trong d6, nguon vi sinh vat ban dia p viing nay la mot nguon dem nang. Nhieu bao cao chi ra ring, nhiiiu loai vi khuan khac nhau o vung re ltia co kha nang hoa tan P v6 cp kho tan nhu dicanxi phot phat. dicanxi phot phat, hydroxyapaut va da phot phat (Xuan et al 2016; Ashrafiizzaman et al.. 2009). Nghien cihi tnroc day cung cho thay vi khoan hoa tan P co tiem nang lam giam .50% lupng P n h ^ ^ ^,^^ | ^ ^ ^ sual cay dong (Uvakush etal, 2014). 6 DBSCL mot so dong Vi khuan hoa tan P d-en dat phen da dupc phan lap (Xuan et al. 2016) va duoc danh (
I Xuan V ' ^ P tren dat noi r h i
CUU. Vi vay, dd tai duoc thuc \
dieu k i ^ d6ng rupng (Ly N g . i ^ T h ^ V h t a f . . ' ^ ^ 2016). Tuy nhien. nguon vi khuSn hoa t phen An Giang d6ng lua mua ndi chua dupc nghi^en"
: hien nham dm vikhuSn NONG NGHI?P VA PHAT TRIEN NON^ffjQN- KY 2 - THAN(5 9/2
KHOA HOC CONG NGHE
hoa tan P tnen vpng tir vimg rd tren dat phen trdng Ilia miia ndi.
2 . PHUONG T r a VA PHUDNG PHAP 2.1. Phirong t i ^
Mdi tnrdng duoc su dung de phan lap vi khuan:
Burk's, NBRIP. Trong dd, thanh phdn cua mdi hirdng Burk's (g L^ gom: 10 sucroza, 0,41 KHaPO^, 0,52 K2HPO4, 0,05 NajSO^, 0,2 CaCl^, 0,1 MgS04-7H20, 0,005 FeS04-7H20, 0,0025 Na2Mo04*2H20 va 20 aga. Tuong ta, thanh phan cua mdi hirong NBRIP (g L') bao gom: 10 glucoza, 5 Ca3(PO,)2, 5 MgCl.-eHaO, 0,25 MgS04-7H20, 0,2 KCl, 0,1 (NH4)2S04 va 20 aga.
2.2. Phuong phap 2.2.1. Phan lap vl khuin
30 mSu dat viing rd cay liia miia ndi duoc thu thap tai xa Vinh Phuoc, huyen Tri Tdn, tinh An Giang. Sau dd, can 1 g mau dat va 99 ml nude cat da dugc khi> tning cho vao binh tam giac va duoc dat frdn may Mc frong 12 gid vdi tdc do 200 vdng/phiit.
Dung dich duoc Idng frong 3 gid, hiit 0,1 ml dung dich nude frong frai ddu fren dia pefri da chuan bi san mdi trudng Burk's khdng N (de phan lap cac ddng vi khuan cd dmh dam), mdi frirong NBRIP chua P khd tan (dd phan lap cac ddng vl khuan hda tan P), d^ khd va u d nhiet dp 30*C. Sau 24 - 48 gid cac khuan lac xu^t hien h-en be mat mdi trudng duoc hdp tuc cay sang dia kliac cho den khi cac khuan lac thuan. Cac khuan lac xuat hien fren mdi trudng Burk's dupc cay sang mdi h-udng NBRIP. Cac khuan lac nay dupc sii dung cho viec danh gia kha nang hda tan P.
2.2.2. Md ta dac diem khuan lac
Md ta hmh thai khuan lac gdm mau sac, hinh dang, dang bia khuan lac va dp noi. Md ta mpt sd d$c diem te hao gom hinh dang va kha nang chuydn dpng ciia khuan lac. Nhudm Gram vi khuan d^ xac dinh gram vi khuan.
2.2.3. Danh gia khi nang hda tan P ciia cac khuan lac diroc phan lap
xac dinh lupng P hoa tan bdi vi khuan frong mdi h-udng NBRIP chua P khd tan. Vi khuan sau khi nudi frong mdi trudng long 48 gid dupc su dung dd danh gia kha nang hda tan P. Dimg 0,5 ml dung dich cua mdi khuan lac cd 0 0 ^ , , la 0,5 cho vao dng nghidm chua san 5 ml mdi tnidng NBRIP long va lac vdi tdc dd 120 vdng/phiit frong 15 ngay. Dung dich ddi chimg la mdi frirdng NBRIP nhung khdng cd sii dung vi khuan. Luong P hda tan b cac thdi diem dupc xac dinh bang phuong phap so mau frdn may quang phd a buoc sdng 880 nm.
3. KET QUA NGHBV CUU VA THAU LUAN
3.1. Phan I§p va d9c tinh ciia vi khudn vung rk cay Ilia miia ndi
3.1.1. Phan lap vi khuan vimg rd cay lua mua ndi
Ket qua cho thay tdng sd 53 ddng vi khudn duoc phan lap tir viing rd liia miia ndi fren hai loai mdi trudng Burk's va NBRIP. Trong dd, cd 40 dong vi khuan duoc phan lap frdn mdi trudng Burk's va 13 ddng vi khuan diroc phan lap fren mdi trudng NBRIP.
3.1.2. Dac diem khuan lac va dSc diem td bio cic dong vi khuan duoc phan lap
Ket qua khao sat dac diem hinh thai ciia cac khuan lac phan lap tren mdi trudng Burk's va NBRIP duoc ghi nhan dbang 1.
B ^ 1. Dac di^m khuan lac diroc phSn lap tir moi tnr6ng Burk's v^ NBRIP Dac tinh theo
dpi
Mau sac Hmh dang Dang bia Do noi
Dac diem khuan lac Trang trong Trang due Vang nhat Trpn Nguyen Mo U i
Sd lupng (Dong)
Tile
(%)
Burk's 12 28 0 40 40 40 0
22,64 52,83 0,00 75,47 75,47 75,47 0
So lupng (Dpng)
Tile
(%)
NBRIP 0 11 2 13 13 13 0
0,00 20.75 3,77 24,53 24,53 24,53 0
Tong cpng (Dong)
12 39 2 53 53 53 0
Til6
(%)
22,64 73,58 3,77
100 100 100 0 Bac diem khuan lac
Mau sac khudn lac: Sau 24 gid nudi cay fren cac
loai mdi trudng cd su xudt hien cac khudn lac cd mau frdng frong, frdng due hoac vang. Khuan lac cd mau frdng frong cd 12/53 ddng, chiem ty le 22,64%, tap
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2019 25
KHOA HQC CdNO W O T ^
trtn ca hai moi tnrong Burk's va NBRIP (Bang !)•
Dac didm te bao cua cac khuan 1^
Khi quan sat te bao dmn kmlt hiJn n quang trung tren mot tnrong Burk's ra khong co dong nao
tu moi u-uong NBRIP. Trai lai, khuan b e co man vang xuat hien tren mpi truong NBRIP la 02/53.
cliu-iu ty le 3,77%, nhung khong co khuan lac man vang nap den tu moi tnrong Burk's. Ddi vm khuan lac CO mau tring due co 39/53, chi^m ly U- 73.."i,-<t Trong dd, 52,82% xuat hien u-en mdi hirong Burk's va 20,76% tren mdi hirdng NBRIP (Bang 1).
Hmh dang khuan lac: Dang iron, bia nguyen va md chiem uu the voi 53/53 dat ty Ip 100%. Trong do, tren mdi dirong Burk's chidm 75,47%: tren mdi trudng NBRIP chidm 24,53% (Bang 1): tuy nhien, k h o n g CO khuiin lac co dang p-on. bia n,t,niycn. lai
Bang 2. B j c d i f a td bao ciia cac khufa Uic da phan Ijp tir indi hTidng Burk's vANBRff hpc. hailpai nhhi chung cac kliufa lac duoc phiin lap tren t mdi ffudng deu co dang hinh que va rat it dong vt khuan cd dang hinh cau (Bang 2). Trong d". te bao dang que chii^m tv Id 9S.11 \ (7S..WI tri'n moi hirong Burk's va 24.53% b-en mdi tntdng NBRIP) va chi co mdt te bao dang hinh cau. chidni ty le 1.89% h-en mdi hirdng Burk's. Da s.) cac ddng ^^ khuin ddu cd kha nang chuyiin dpng tren ca h,li loai mdi hudng.
Dac tinh theo dpi Hinh dang
Dac dit?m khufa lac
Que Ciiu
So luon.ii Ti le (Dong) (%1_
Burk's 73..TK
So lu^lg (Ddng)
ri IP
(%)
39
0 O.lKl
long cpng (Ddng)
52 1
Tile
(%)
18.11 1.8
Chuydn dong Co 40 ^^•i' ! 13 24.53 53 100
3.2. Danh gia kha nang hda tan P cija vi khufa vimg rS trtn d^t trdng lua ndi
:12.I. Kha nang hda tan P ciia cac khuiin I$c duvt'pbiui lap Iren nidi trudng Burk's khdng dam
Bfag 3. Kha nang hda tan P (mg P A L') dia cac khufa lac dupc phfa l$p trtn mdi truimg Burk's
khpng dam N'uay siiii_klmi_
1 Thong sd _ .'i_ _ l l l _ l". L'll_
' lhimlm)n,i,'l'.0 (mg/L)
hoa tan P. cac ddng Co kha nang hda lan P cao nhat vao thdi didm 15 NSII dtroc the hien o hinh 1. Trong dd. nam dong cd Itrpng P hpa tan cao nhiit la 22-BD6.
23-BDS. 27-BD6a, 24-BDlic va 10-BD6b vdi ham lupng P Ciia 11 ddng vi khufa dao dong 14.15 - 16.51 m g P . O . L ' .
Nlio nhat , T n m . o i
|Lon nil.It
|Triiiui b i n h Sai sd chuan
3.(i9 -L.'il li.34 l.iili 0.01
1.09 ."1.29 7.9,^
."i.47 0.02
,-i.."i9 7.23 1 2 . , ^ ,S.I7 lli..M l.'i.02 12.:',:; 9.97 D.IIti 9.09 Kdl qua phan lap vi khuan tren mdi trudng Burk's: Tat ca cac khuan lac duoc phan lap tir vimg rd Ilia ndi fren mdi truong Burk's cd kha nang hda tan P. Tuy nhidn. kha nang nay d cac ddng vi khuan la rat khac nhau. Ket qua hda tan P vao thoi diem 5 ngay sau khi li voi gia tri nho nhat, trung vi. ldn nhdt va trung binh ldn luot la 3.69. 4,5-1. 6,34 va 4.66 mg P.O. L'(Bangs).
Tuyen chon vi khuan hda tan P duoc phan lap tu mdi tnrong Biuk's: Tat ca 40 khuan lac viing rd duoc phan lap fren mdi trudng Burk's deu cd kha nSng
Hmh 1. Kha nSng hda tan P (mg P^Os L') cua cdc khudn l9c fri^n vpng dupc phan lap frdn mdi frudng
Burk's sau 15 ngay ii
Danh gia kha nSng hoa tan P theo thdi gian cua cac khuan lac duoc phan lap fren mdi frudng Burk's- Trong 5 ddng vi khudn dugrc chon cd ham luong lan theo thoi gian dupc the hidn b hinh 2 Trong do dong 10-BD6b cd kha nang hoa tan P trung binh cao nhat frong 5 ddng vi khuan nay (Hmh 2)
N O N G NGHIEP VA PHAT TRIEN N O N G THON - KY 2 - THA' "• 9/5
KHOA HOC CONC NCHE
Himlups P frngPiOsL-il 20-1
15 101
5
-22^06 -»-23-BD6 -2"-Bi>j -•~;-;-BD&
-IO-BD6b
mdi tnrpng NBRIP ddu cd kha nang hoa tan P. Ket qua 5 NSU cho thay, kha nang hda tan P thap nhat, tnmg bmh va cao nhat Ifa Iirpt la 3,74, 4,19 va 4.69 m g P , 0 5 L ' ( B f a g 4 ) .
Bang 4. Kha nang hda tan P (mg PjOs L') a i a cac khufa lac dugc phfa Ijlp trtn mdi hudng NBRIP
10 15 20
Ngai sau khia Hinh 2. Kha nang hda tan P (mg PaOg L'O aia cac khufa
1 ^ tiifa vpng dugc phfa lap trdn mdi tnrdng Burk's Cac ddng vi khufa cd kha nang hda tan P khd tan cd xu hudng chung la tang dfa qua cac ngay 5.
10.15vagifaid20NSU.
3.2.2. Kha nang hda tan P cua cac khuan lac duoc phan lap tren nidi tnidng NBRIP
Kdt qua phan lap vi khufa tir vung re tren mdi hirdng NBRIP; Tong sd 13 ddng vi khufa phfa lap hr
Ham lirimg P (mgPiOsL-') 10.0-1
7 . 5 -
5.0
0.0^ • • • - • - • •
20-NI16 25-ND6b 4-NMa 28-ND6b 22-Nr)6a 28-NDda 3-ND6 Thong sd
Nhd nhat Tmngvi Lon nhat Trung binh Sai sd chufa
Ngay sau khi li 5 1 10 j 15 1 20 Ham lupng P2O5 (mg/L) 3,74
4.20 4,69 4,19 0,01
3,66 4,15 5,20 4,17 0,01
3,23 4,27 7,13 4,60 0,03
4,25 5,03 6,76 5,29 0,02 Tuyen chon cac ddng vi khudn ti^m nang hda tan P duoc phan lap tir mdi trudng NBRIP: Trong 13 ddng vi khuan viing rd cd kha nang hda tan P frdn mdi trudng NBRIP, 10 ddng cd kha nang hda tan P cao nhdt vao thdi diem 15 ngay sau khi u dugc the hidn d hinh 3.
23-ND6 24-ND6 i-ND6a Dong vi khuan Hinh 3. Kha nang hda tan P (mg P2O5 L"^) a i a cac khuan lac tri^n vpng dupc phan lap frdn mdi trudng NBRIP
sau 15 ngay u Danh gia kha nang hda tan P theo thdi gian cua cac ddng vi khudn dupc phan lap fren mdi trudng NBRIP: Ddi vol cac ddng vi khudn nay, 3 ddng vi khuan cd kha nang vugt frdi nhat (28-ND6b, 3-ND6 va 4-ND6a) duoc chpn cho danh gia kha nang hda
tan P theo thdi gian. Kha nang hda tan P cua hai ddng vi khuan 28-ND6b va 3-ND6 cao khac biet y nghia thdng ke 5% so vdi cac nhdm vi khuan cdn lai vdi ham luong P duoc hda tan 6,33 - 7.13 mg P^O^ L', ke ddn la ddng vi khuan 4-ND6a (4,98 mg P p ^ L').
N O N G NGHIEP V A PHAT TRIEN N O N G THON - KY 2 - THANG 9/2019
KHOA HOC C 6 N C NCHE trung tren mdi trudng Burk's va khdng cd ddng nao
tu mdi Uirong NBRIP. Trai Iai, khufa lac cd mau vfag xuat hifa tren mdi Uitdng NBRIP la 02/53.
chidm ly Id 3.77%, nhung khdng cd khufa lac niau vang nap den tu mdi tnrong Burk's. Ddi vdt khufa lac cd mau t r ^ g due cd 39/53, chiem ty le 73.."K^-%.
Trong do. 52.<S2^i xuat hien hen moi dirdng Burk's va 20.76% h-en moi trudng NBRIP (Bfag I).
Hinh dang khufa lac: Dang p-dn. bia nguyen va mp chidm uu did vdi 53/53 dat t>' Ip 100%. Trong dd, trdn mdi trudng Burk's chiem 75,47%; tren mdi tnrong NBRIP chiem 24..">3X (Bang 1); hiy nhien.
k h o n g CO khufa lac cd dang tron. bia nguyen. Iai Baiig 2. Dac^didm^t^ bfa Clia^cac khufa lac
So luong Tl le (l)ong)___ (%)
trtn ca hai nioi tradng Burk's vi NBRIP (Bans D- Dac diem le bao i ii.i cac khuiin Ix Khi quan sat td bao dudi kinh ludn vi quang hoc nhm Chung cac khufa lac durx- phan lap Wn hai ipai mdi hudng ddu cd dang hinh que va rSt it dong vi khufa CO dang hinh riu (Bang 2). Trong do. te bao dang que chiem tv le 98.11% ("vV'-^*' n*n nlol duong Burk's va 24.53% U-en mdi Uiidng NBRff) va chi cp mdt te bfa dang hinh cju. clm-n. ly W LS"* « " ™ ' dirdng Burk's. Da sd cac ddng vi kliufa deu cp kha nang chuyen ddng tren ca hai loai mdi trudng.
) D^c tinh theo 1 doi
Hinh dang IChuven (Inn
D$c dieni khufa lac
Burk's
da phan I4p tir mdi hirdng Burk's^vi NBRIP So linnlg n Ic roll^f
(Ddng) I (%) 1 cgng NBRIP 1 (Ddng) One
Call C.I
39 1 40 3.2. Dfah gia kha nang hda tan P ciia vi khufa viing th trdn d^t ti^ng ltia ndi
3.2.1. Klia nang hda tan P cua cac khuan l^c duoc phan lap Iren mdi Irudng Burk's khdng dam
Bfag 3. Kha nang hda tan P (mg PjOj L") cua cac khufa lgc dupc phfa l$p trtn mdi trudng Burk's
khdng dam Tlidng sd
Nhd nhat T i l i n g VI Um nhat Tiling binh Sai si) chuan
Ngay sau klu u J ) _ „ 1 0 ^ 1 . , 1 5 ^ _ 2 0 _ _ Ham Immg P.O. (iii.i;/!.) ' 3.69 ] 4.09 ! ,S.59 ' 7.23 4.:",4 , ,).29 !l2..'J9, 8.47 6.34 1 7.98 116.511 18.02 4.(;6 1 5.47 il2.;B 9.97 0.01 j 0.02 ' O.lKi 0.09 Ket qua phfa lap vi khufa tren mPi tnrdng Burk's: Tat ca cac khuan lac dtrpc phfa lap hi vung re lua ndi trdn moi tnrong Burk's cd kha nang hoa tan P. Tuy nhien, kha nang nay d cac ddng vi khufa la rat khac nhau. Kdt qua hda tan P vao thdi dicm 5 ngay sau khi li vtn gia hi nhd nhat, trung vi. Ipn nhdt vii trung binh Ifa lupt la 3.69, 4.54, 6,34 va 4.66 mg P , 0 s L ' ( B f a g 3 ) .
Tuyen chpn vi khufa hda tan P diroc phfa lap tu nidi tnrdng Burk's: Tat ca 40 khufa lac vimg re duoc phfa lap trtn mdi tnrtmg Burk's deu cd kha nang
13 13
24.53 0,00 24,53
52 1 53
Tile
(%)
1.8 100 hda lan P. cac ddng co kha nang hda tan P cao nhat vao Ihdi didm 15 NSU dupc the hien p hinh 1. Trpng dd. nam ddng cd lupng P hda tan cao nhat la 22-BD6.
23-BD6, 27-BD6a, 24-BD6c va 10-BD6b vol hfal lupng P ctia 11 ddng vi khufa dao ddng 14,15 - 16,51 m g l ' . 0 , 1 . ' .
Kuift ri unuan
Hmh 1. Kha nfag hda tan P (mg PjO, V) cda cac khufa lac tridn vgng dupc phta lap trta mdi trudng
Burk's sau 15 ngay li
Dfah gia kha nang hda tan P theo thdi gian cua cac khufa lac dupc phan lap trta raoi huong Burk's- Trong 5 ddng vi khufa dupc chgn cd hara luong lan theo tho, gian dupc the hien 6 hinh 2 Trong dd dong iaBD6b cd kha nang hoa tan P p-un, binh ca,;
nhat h-ong 5 dong vi khuan nay (Hmh 2)
N O N G NGHIEP VA PHAT TRIEN N O N G THON - K'i' 2 - THANG 9/5
KHOA HOC CONC NCHE
HamliTTDg P tmgP,OsL-'l 20 1
-;;-BD6 -1-23-61)6 -r-BD6a -•-24-BD6C - ll)-BD6b
m d i h i i i m g N B R I P d d u cd k h a n a n g h d a tan P. Ket q u a 5 N S U c h o thjiy. k h a n a n g h d a tan P t h a p n h a t , h i m g b m h va c a o n h a t Ifa lupt la 3.74. 4.19 va 4,69 m g P , 0 s L ' ( B f a g 4 ) .
Bfag 4. Kha nang hda tan P (mg PjOj L") ctia cac khufa lac dupc phfa l^p trdn mdi truimg NBRIP
5 10 15 20
Ngay sau khi li H]nh2. KhananghdatanP&ngP205L-') ctta cac khufa
I9C trifa vpng diBpc phfa lap trdn mdi tnrdng Burk's Cac ddng vi khufa cd kha nang hda tan P khd tan cd xu hudng chung la tang dfa qua cac ngay 5.
10.15 va giam d 20 NSU.
3.2.2. Kha nang hda tan P cua cac khuan lac duoc phan lap tren mdi truong NBRIP
Ket qua phan lap vi khufa tir vung rd trdn mdi trudng NBRIP: Tong sd 13 ddng vi khuan phfa lap hi
Ham lutmg P (mgPAL-') 10.0-
7.5
Thong sd
Nhd nhat Tnmgvi Ldn nhat Trung binh Sai sd chuan
Ngay sau khi u 5 1 10 1 15 1 20 Ham lupng P2O5 (mg/L) 3.74
4.20 4.69 4.19 0,01
3,66 4,15 5.20 4.17 0,01
3,23 4,27 7,13 4,60 0,03
4,25 5,03 6,76 5,29 0,02 Tuyen chgn cac ddng vi khuan tidm nang hda tan P duac phan lap tir mdi trudng NBRIP: Trong 13 ddng vi khuan vting rd cd kha nSng hda tan P trdn mdi trudng NBRIP. 10 ddng cd kha nSng hda tan P cao nhat vao thdi diem 15 ngay sau khi u dugc the hidn dhinh 3.
5.0
2.5 -
0 . 0 - ^ •
20-ND6 25-ND6b 4-ND6a 28'NT)6b 22-ND6a 23-ND6 24-ND6 l-ND6a Dong vi khuan Hinh 3. Kha nang hda tan P (mg P2O5 L^ cua cac khuan lac tri^n vgng dugc phan Mp trdn mdi tinrdng NBRIP
sau 15 ng^y u
tan P theo thdi gian. Kha nang hda tan P cua hai Danh gia kha nang hda tan P theo thoi gian cua
cac ddng vi khuan dugx: phan lap tren mdi trudng NBRIP: Ddi vdi cac ddng vi khu^n nay, 3 ddng vi khuan cd kha nang vupt h-gi nhat (28-ND6b, 3-ND6 va 4-ND6a) dupc chon cho danh gia kha nang hda
ddng vi khuan 28-ND6b va 3-ND6 cao khac bidt y nghia thdng ke 5% so vdi cac nhdm vi khuan cdn lai vdi ham lupng P duot: hda tan 6,33 - 7,13 mg P2O5 L-.
ke den la dong vi khuan 4-ND6a (4,98 mg PPO^ V-).
NONG NGHIEP VA PHATTRIEN NONG THON - KY 2 - THANG 9/2019
KHOA HOC CONC H ^ . . .
Hamlir7ii£ P ImtPAl 'i
Hmh 4. Kha nang hda tan P (mg PjOs L') theo thdi gian cua cac khufa lac hifa vpng d u ^ phfa 1 ^ trdn
mdi dndng NBRIP
Kha nang hda tan P theo thoi gian ciia ba ddng vi khuan lidm nang dupc the hien d hinh 4. Trong do.
ddng vi khufa 4-ND6a co kha nang hoa tan P tang dfa tir 4,06 mg P2OS L' vao thdi diem 5 NSti den 5,46 mg PjO, L' vao Ihtn diciii 2(1 NSU: tuung tu, dong vi khufa 3-ND6 tang tir 3,87 ddn 6,35 mg P;(). L' theo cung didi didm, hong khi dong vi khufa cdn lai 2.^^
ND6b cd hfal lupng P hda tan chi tang 6in 15 NSl*
va giam dan pgiai doan cdn Iai.
Ngoai ra. khi di.ia h-dn ket qua hda tan lan trung binh sau 20 ngay u. Ddi vdi 11 ddng vi khufa hem nang dirpL- chpn tir moi tniung Burk's. ddng 3-BD6b.
34-BD6a va .5-BDfib co kha nang hda t:ui cao trong khi 10 ddng VI khuiin tiem nang dupc phan lap tir mdi tnrdng NBRIP gdm 28-ND6a cung co kha nang hoa tan cao. Day cdng la cac dong vi khufa hdm nang can dupc ii,trhien ctru.
4. KH LUAN
Ket qua phfa lap dupc 53 ddng vi khufa d cac mpi tnrdng Burk's va NBRIP. Trong do. nam dong M khuan co luong P hda tan cao nhat tu moi tnrpng Burk's la 22-BD6. 23-8110. 27-BD6a. 24-BD6c va 10- BD6b voi lurnig P hda tan 14.15 - 16.51 mg P,Os L'.
Ngoai ra, klia nang hda tan P ciia hai dong vi khufa 2.'vND6b va 3-ND6 dupc phfa lap tir moi tnrong NBRIP cao kliac blpt y nghia Uiong kd so voi cac ddng VI kliufa cdn Iai vol ham Itrong P hda tan 6,33 • 7,13 mg P..O5 L' sau 15 ngay u.
De nghi dinh danh ba ddng vi khufa vung re lua mua ndi co tridn vpng nhat 10-BD6b. 2tvNI)6b va 3- ND6.
Tti UEU THAM n u o
1. Ashraftizzaman M.. Hosscn F. \ , Ismail M R- Hoque A.. Islam M. Z., Shahidulkdl ^ M- ^'''""
(2009). Efficiency of plant gi,»lh-prom»""«
rhizobacteria (PGPR) for the enliaiuement of nce growth. African Journal of Biotechnoli>g>'- ^O)-
2. Cordell D.. Drangert J. O.. W-hite S. (2009)- The story of phosphorus: global food security and food for thought. Global environmental change. 19:
292-305.
3. Daniels C . Michan C . Ramos .1. L. (2009) • New molecular tools for enhancing methane produchon. explaming thermodynamically limited hfestyles and other important biotechnologieal issues. Microbial biotechnology. 2(5): 533^.536.
4. Uivalmsha J. Y., Vermab J. P.. Jaiswalb D. K., Kuniarc A (2014). Evaluahon of PGPR and different concenhahon of phosphonis level on plant growth, yield and nuti-ient content of rice (Oryza sativa). Ecological Engineering. 62: 123-128.
5. Ly Ngoc Thanh Xufa. Trinh Quang Khuong, Le Van Dang, Trfa Van Ddng va Ngd Ngpc Hung (2016). Higu qua cua vi khufa npi sinh Burkholderia vielnailiieiisis\ei\ sinh trudng va nang suat Ilia trdng h-en ba Wing dat phen ddng bfag song Ciiu Long.
Tap chi Klioa hgc - Tmdng Dai hpc Cfa Thp. 44b: 1- ,s
6. Miller S. H.. Browne P.. Prigent-Cambarel C . Combes-Meynet E.. Morrissey J. P., O'Gara F.
(21110). Biocheniical and genomic comparison of inorganic phosphate solubilisahonin Pseudomonas species. EnvironmenUil microbiology reports 2(3)- 103-411.
7. Sashidhar B.. Podile A. R. (2009). Transgenic expression of glucose dehydrogenase in Azolobacter vineladu enhance esmmeral
growth of sorghum biotechnology. 2(4): 521-529.
S^Sharma S. B., Sayyed R. Z.. -Invedi M H sustainable approach for managing phosphoms deficiency ,n agncultural soils. Spnngerplus 2 ( 1 ^ 7
9. Srivastava A. Chun S. J.. KoS.R Kim T 41, c . v . . o h H . M ( 2 „ i ^ . P , „ , , „ , , „ , , , _ , . ; ; , ^ ^ ' ^ ^ ^ ^ > ; for nutnent remediation .,„d fee,! :.r„duc,io„'l™
solubilization and seedlings. Microbial
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2
^1 - K -< .i - ,,
KHOA HOC CONO NGHE
eutrophic lake. Journal of Envu-onmental 11. Xuan L N. T.. Dung T. V.. Hung N. N.. Diep Management 203:342-348. C. N. (2016). Isolation and characterizaUon of
10. Toure A. L, M. A Choudhury, M. Goita, S. rhizospheric bacteria in rice (Oryza sativa L) KoU, and G. A. Paku (1981). Gram yield and yield cultivated on acid sulphate soils of the Mekong cpmppnents of deepwater rice ta West Africa. Pp: 103- D « " ^ Vietaam. World Journal of Phannacy and 112. Proceedmgs of the 1981 hitemational Phannaceuhcal Sciences. 5(9): 343-358.
Deepwater Rice Workshop. 1982. IRRl, P.O. Box 933.
Manila. PhiUppmes.
ISOLATION, SEDECnON AND EVALUATION OF PHOSPHORUS SOLUBIUZING CAFACnY OF BACTERIA ISOLATED FROM RHIZOSPHERIC ACID SULFATE SOIL CULTWATED
FLOATING RICE
Ly Ngpc Thanh Xuan, Le Vinh Thuc, Nguyen Quoc Khupng Summary
The floating nce is conserving in An Giang that associated wilh the maintenance of the community of microorganisms. This research was conducted to select the potenUal rhizosphenc bacteria from acid sulfate soil cultivating of floating nce possessing the ability of phosphorus solubilization. The resulls showed that 53 colonies were isolated rhizosphenc soil from Burk's and NBRIP media. Of those. 5 colonies producing the highest phosphonis concentration were selected as strains 22-BD6. 23-BD6, 27- BD6a. 24-BD6C and lO-BD6b 14.15 - 16.51 mg P,Os L' from Burk's medium. Besides. Uie capacity of P solubiiizing of shains 28-ND6b and 3-ND6 isolated from NBRIP medium were signiiicanUy higher than odier colonies wiUl 6,33 - 7.13 mg PjOs L' after incubahng 15 days. It should be idenUfied die selected bactena and apply under nethouse or field to reduce the volume of phosphorus for plant.
Keywords: Acid sulfate soil, floaling rice, rlnzospheric bacteria, phosphorus solubilization.
Ngudd phfa bidn: TS. Biu Huy Hifa Ngay nhfa bfa 5/7/2019 Ngay thdng qua phfa bifa: 6/8/2019 Ngay duyet dang: 13/8/2019
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2019
