• Nghien ciru - Ky thuat
Summary
A series of biodegradable hydrophobic polymer cholesteryl dendrimer films for loading metronidazole was produced varying mole ratios of dendrimer G5 and cholesteryl ehloroformate, as a topical carrier for periodontal treatment.
The erosion of the polymer film and dmg release pmfile were characterized in-vivo by the dorsal rat model. The obtained earner proved no toxicity on the experimental animals, and both polymer emsion and drug release rates decreased with increase in hydrophobicity of the cholesteryl dendrimer G5 1/10 containing 5%
metronidazole (w/w). Cholesteryl dendrimer G5 1/10 film prolonged the dmg release over up to 4 days and efheiently retained the dmg concentration in therapy range; thus, the obtained polymer film showed suitabie for the topical periodontal treatment
Keywords: Cholesteryl dendrimer G5, metronidazole, in-vivo.
Tai li^u t h a m khao 1. D l Tnjng Dam (1996), Phuang phap xdc i^nh dflc tfnh cap cua thuoc, NXB Y hpc Hd Npi, tr. 127 -132.
2. Trln Huu Dung (2012), "Che tgo film copolymer Dendrimer G5 Pluronic F127 piiong thfch metronidazole keo d&i", Tgp chi Dugc hgc, so 435, tr. 42.
3. El-Kamel /\mal Hassan, Y./tehri Lubna, and A.AIsarra Ibrahim (2007), "Mionmatrical metronidazole Benzoate Film as a Local
mucoadhesive Delivery System for treatment of Periodontal System", AAPS Pharmaceutical Science Technotogy, No. 8 (3), pp.184 - 194.
4. Emami J., Varshosaz J., and Saljoughian N.
(2008), "Development and evaluation of controlled- release buccoadhesive verapamil hydrochloride tablets", DARU Joumal of Pharmaceutbal Sciences.
No. 16(2), pp. 6 0 - 6 7 .
5. Kelly H.M., Deasy P.B., Ziaka E., et al. (2004),
"Formulation and preliminary in vivo dog studies of a novel drug delivery system for tiie treatment of periodontitis", Internatbnal Journal of Pharmaceutics.
No.274, pp. 167-183.
6. Wmberiy / ^ n Gates (1999), "Controlled Drug Delivery Using Bioerodible Polymeric Systems for the Treatment of Periodontitis", Department of PhanrmceiAK^I Sciences, UniverstyofToroTto, pp. 37 -108.
7. Parthasarattiy Belsen David J and (2011),
"Formulation and in-vitro evaluation of Bio-degradable polymer based Sparfloxacin Periodontal chip", Researdi Joumal of Pharmaceutbal. No. 2(3), pp 319-325.
8. Thulasamma P. and Venkateswarlu P. (2009),
"Spectrophotometric method for the determination of metronidazole in phamiaceutical pure and dosage forms", Journal of Chembal. No. 2(4), pp. 865 - 868.
9. Magnusson Ingvar (1998), "The use of locally delivered metronidazole in the treatment of periodontitis. Clinical results", Joumal of Clinical Periodontal. No. 25(11 R 2 ) , pp. 9 5 9 - 9 6 3 .
10. Tran Huu Dung, Hoon Yoo (2008),
"Preparation and evaluation of cholesteryl PAlWlAM dendrimers as nano delivery agents for antisense oligonucleotides", Joumal of Physicochemical &
Engineering Aspects. No. 313, pp. 2 7 3 - 2 7 7 .
o r A
Kiem nghiem rau dang va rau dang bien bang phircng phap Men vi
Le Tlii Huyen Trang, Nguyin Twdng Vy, Nguyen Vi^t Than Trudng Dgi hgc Duac Hd Ngi
Dat van de
Rau dang Id m | t logi d u y c lieu quen thufic vdi ngudi dan V i p t N a m , d u a e s d dung r i t pho b l l n d l Idm t h u l e , rau an. 0 cac dja p h u a n g ten rau d i n g d u y c dOng d l eh? mflt s6 loal khdc nhau, trong dfl co vdi lodi cfl gid tri s d dung cao, da vd dang d u a c the gidi quan tam nhu rau
dang ho Rau ram (Polygonaeeae), rau dang d i t thupc hp Rau d i n g d i t (Aizoaceae), rau ddng b i i n thuflc hp Hoa mom ehfi (Serophulariaeeae), rau dang n u d c t h u d n g gpi Id rau x u a n g cd thufle hp e l m c h u d n g (Caryophyllaceae). Do deu d u y c gpi Id rau d i n g nen trdn t h y c t l sit dijng nhleu khi bj n h i m lan.
T ^ CHf DlTQC HQC - 03/2013 (SO 443 NAM SSL
• Nghien CCPU - Ky thuat
Cdc cdy ndy khi con tuai efi t h i d l ddng nhdn bilt bing mat thudng, nhung khi da khfl chl bien, sdn xuit thanh cdc chl phim thi rit khfl phdn bl$t. Bdi bao nay xay dyng ca sd dd li0u d l phdn bigt rau dang vd rau ding biin dang duyc sd dgng tren thj trudng d dgng dupc lipu hay chl phim.
Ooi tu'O'ng va p h u v n g phap nghien ciru
Nguydn li^u
Ode mlu rau dang thu hai vao thdi gian efl hoa tgi Gia Ldm - Hd Nfli trong khoang thdng 6-7 nam 2010, 2011. Cac mlu rau dang blln ttiu hdi vdo thdi gian cfl hoa tgi Mul Nd, ttnh Binh Thuan trong khoang thdi gian thdng 9-10 nam 2010. 2011.
Cde mlu tuai dupe phal d y khfi, cho vdo cdc tui PE dfing kin, d l nai thoang mdt, khfi rdo.
Thiet bi nghien cdu
Cdn k9 thu$t Preeisa, bp ray, tu d y Shellab, kinh hiln vi sinh hpc, chyp anh chuyen dyng,..
Phuvng phdp nghidn cdu
Sd dgng phuang phdp nghidn edu hiln vl thudng qui.
Quan sit d$c diem hinh thai
Mfi td hlnh thai: Quan sat, chgp dnh cdy tgi thye dja, quan sdt, phan tfch, chup dnh ea quan sinh sdn, gidm djnh ten khoa hpe eua edy.
Ldm vi phiu vd soi bfit duyc lieu.
Viphau
Dupc li^u tuai hay khfi da ngdm mim d t ngang bang dgng eg d t vi phau d m tay, chpn cdc Idt d t mflng.
- Tly cdc Idt d t bang nudc Javen hay dung djch cloramin (20-30 phut). Rda bang nude d t .
- Ngdm acid acetic 5% (15-30 phiit). Rda bing nude d t 3 lln.
- Nhufim vi phau bang xanh methylen (1-2 phot). Rda bang nudc d t .
- Nhufim dfl son phen (2-10 phiit). Rda bing nude d t .
- Ldn vi phiu trang glycerin, quan sdt trdn kinh hiln vl, chpn cdc phin diln hlnh d l chyp dnh.
D^G diim b^t
Duyc ll^u tdn thdnh bpt, ray qua rdy s6 150Mm.
- Ldn bflt trong nude, quan sdt trdn kfnh hiln vl, chpn ede d$e diem dien hinh d l chyp dnh.
- Anh cde dde dilm bflt duyc tUp hyp thanh nhfim theo dp phflng dgi.
K i t qua va ban lu$n Hinh thai thiPC v^t Rau dang
' Cdy CO, song hdng nam, dai 10-40 cm, phdn cdnh nhieu. Than manh cfl khfa dpe, nTiilu dit, Iflng rit ngan. Re chinh mdnh, nhieu r l phu. La nhfi mpc so le, hlnh thufln hgp, hogc hinh mui giao ngupc, kich thudc ddi 0,5-1,5 em rflng 0,2- 0,4 cm, mfing, hai mat nhan, gdn bdn khfing efi, chfip nhpn hoae hi, gin nhu khfing efi cuflng.
Be chia dai 0,2-0,3 cm, mdng mflng, mau tring, trong suit. Hoa nhfi mau hong, mpc ty 3-5 hoa trong mfli ta bic; la bac hlnh pheu, mep vat.
Hoa deu, ludng tinh cuong khflng thfl ra ngoai Id bac, mem, cfl mlu d giua. Bao hoa 5 mdnh, glc dfnh nhau ft, trdn 5 thOy sau, hinh thufln, dai 0,1-0,2 cm. Nhj 5-8, thudng ngdn han hoa, goc oh] nhj phinh hai to, bao phin dinh glc, hudng trong, md theo khe dpc. Blu thuyng, hinh trdng, 3 canh, 3 Id nodn, 1 fl, efl 1 noan, thing, dfnh lung, vfii nhgy 3, ho^c dfli khi 2, rdi nhau hoac dfnh nhau d phin dual. Qua be, hlnh trdng rflng, 3 canh, mdu nau den, nhan bflng, duae bao trong bao hoa khflng dong trudng.
Can ed vao eac tai li$u phdn logi thye vat:
Flore general de L'lndo - Chine'^'^, Tflm tat dae dilm ede hg edy thule'^\ Cay cfi Vipt Nam'^'^' ehung tfli djnh tdn khoa hpe cQa edy nghidn edu la Polygonum avieulare L., ddy la cay thuoc lau nay van dupe sd dung trong y hpc dan tpc vdi tdn gpi rau dang, biin sue. Theo Thye vdt ehf Viet Nam^^^ vdi J kiln cOa Tien sy Tran The Bach (Vign Sinh thdi vd Tdi nguydn Sinh vat Vidt Nam) cay nay dupe xdc ^nh Id Polygonum plebejum R. Br, hp Rau ram (Polygonaeeae) (hinhl).
Rau dang biin
Cay thdo, sing lau nam, cao 10-20 cm. Than nhan mau xanh, thdn non dfli khi cfi mau hai nau do, tiet dign trfln, mpng nudc, phan goc mpc bo, ben r l d nhdng mlu, phan tren mpc ddng. La dan, mpc dli, khfing efi Iflng, mpng nudc, hinh trai xoan, ddi 0,8-1,2 cm, rflng 3-5 mm, mep nguydn, glc thufln, diu tu, hai mat nhan, chf gdn giua rfl. Hoa ludng tinh, mdu
T ^ CHl DlTQC HQC - 03/2013 (S6 443 NAIM 53)
• Nghien CIFU - Ky thugt
trang, mpc dan doe d ke la tren mflt cuong dai, hai la bac eon hlnh spi d dfnh cuong hoa. Bao hoa gom 5 Id ddi rdi, khfing diu nhau, la dai sau to nhit, hinh trdng cfl 5 gdn chfnh, hai Id ddi toFdc hlnh trdng, mui nhpn, 3 gan ehinh, hai la ddi bdn nho nhat, hlnh dai, 1 gan. Cdnh hoa 5, moi canh hoa cfl 3 gdn, dinh vdi nhau ben dudi tao thanh Ing. Trang dai gip hai lln ddi. Nhi 4, khflng deu, ehi nhj nhan. Bao phin 2 fi rdi, xep song song cgnh nhau, md dpe, hudng trong, dinh day. La noan 2, dfnh nhau thanh blu tren 2
Hinh 1: Ciy rau dang [Polygonum avieulare L.) Dya vdo dgc diem thyc vgt ta phdn bipt duyc 2 loal nay: Rau dang than efi khia dpe, phan dot vdi cdc dflng ngin. Id mpc so te, philn Id hpp, cfl be ehia, hoa nhfl mdu hong nhat, 3-5 hoa trong mli la ble. Rau dang blln than tron, nhan, mim, la mpc doi, philn Id mpng nude, hoa dan dpc, mdu tring, 5 ednh, mpc trdn mflt eulng dai.
Vi phau Rau dang Viphiu thin 32
d, mil o chda nhilu nodn dinh nodn try. Vol nhgy 1. Qud nang, hinh trdng nhan, cfl ddi tin tai. Hgt nho cfl canh (hinh 2).
Cdn ed vdo cdc tai li§u phdn logi thyc vdt : Flore general de Undo - Chine'^'®^ Tom tat dab dilm ede hp eay thuflc^^^ Cay co Vi#t Nam^^.
Vdi sy tu vin eua Tien sy Trln T h i Bach (Vign Sinh thai vd Tai nguydn Sinh v#t Viet Nam) chiing tfll xac djnh tdn khoa hpc eiia mlu nghidn edu la Bacopa monnieii (L.) Wettsfc, hp Hoa mom cho (Serophulariaeeae) (hlnh 2).
Hinh 2: Ciy rau ding bien [Bacopa monnieri (L.)Wettst.]
fl^c diem vi phau: Mat d t hinh trfln, co nhung mlu ill. Td ngodi vao trong efi: Ldp bieu bi (1) gom mpt hdng te bao kfch thufi'c ldn, khfing deu, co nhung mlu loi (2), thdnh ngodi t^
bdo hfia eutin. Mfl ddy gom 2-3 ldp te bdo thdnh ddy, tup trung thdnh ddm d nhdng mau ill. 1^6 mem vo (3) gom vai Idp t l bao thdnh mong, trong mfi mem vo sdt libe cfi syi xip thanh tdng ddm (4). Libe (5) tao thanh vdng bao quanh mfl go. G 5 (6) efi cdc mach g l xip thanh hdng, cac hdng t|p hyp thdnh bfi trong mfi g l . Cac t l bdo mfi mim ruOt trfln, da gide, thdnh mfing, cfl kfcti thudc Idn (7) (hinh 3).
T ^ CHf DlTQC HQC - 03/2013 (SO 443 N A M « ,
• Nghien CLFU - Ky thuat
Vi phau li
Phin gdn Id: gdn tren phang, gan dudi hai ill, bilu bi tren vd bieu bi dudi gom cde t l bdo Idn. Bfl libe go gan ehinh gim cfl cung libe d ngoai om lly cung go nho d phfa trong.
Phin philn la: Bilu bl trdn va bieu bi dudi d u tao bfii mflt hdng t l bao eo kfch thudc ldn. Mfl gi^u gim hai hang te bao hinh did nhat xip ddng, mfi khuylt gom cdc t l bao thanh mfing xip Ifln xfln rdi rde efl cac tinh the ealei oxalat hlnh d u gai.
Rau dang biin Vi phau thin
IVIat elt hinh tron td ngoai vao trong efl: Bilu bi gim mflt hdng t l bdo nho, hinh trfin xep deu
A.:^^^^S^p^
Hlnh 3: Viphiu than rau ding Dya vdo dgc diem vi phSu than. Id cfi t h i phdn bi^t duyc hai lodi nay: Vi phiu thdn rau ding bleu bi efi eae u loi, mfl day tap trung tiidnii tung dam nhfi, syi tgp trung thdnh bfl, phin libe-gl nam phin ngodi thdn, mfi mem mfit Idn. Vi phau than rau ding bien cfi bilu bi nhan, mfl ddy tgo thdnh vong dudi bilu bi, khfing cfi cdc bfi syi, libe-gfl tao thanh vflng d gin trung tdm vi phdu. Vi phdu Id rau dang d phin gdn giua gan dudi III nhieu, vl phdu la rau dang biin gdn dudi va gdn trdn gin nhu phIng,
fi$c diem b$t Rau ding
dan (1). Mo ddy gom 1-2 hang t l bao thdnh ddy hlnh trfln (2). Mo mem vo (3) gem cde t l bao thdnh mfing hinh trfln hogc da giac, cfl eae khoang gian bdo Idn (4). Phia trong mfi mim vfl la try bi (5). Sdt toi bl la libe (6) tao thdnh vong bao quanh vfing gfl (7). Mfl mim rupt d giua (8), gom nhilu t l bao hinh tron, kich thudc khflng diu nhau (hinh 4).
Vi phiu la
Bieu bi trdn va bilu bi dudi gim mflt hdng te bao hinh trfln xip deu dgn.Mfi mem gom cac t l bdo hinh trfln, thdnh mfing. Libe-go: Libe gom cdc t l bao nhfi xep thanh vfing bao quanh mfl gfl. Gfl CO eae mach go.
Hinh 4: Viphiu thin rau ding biin Bflt mdu ndu xdm, mCii tham, vj ding. Quan sat dudi kfnh hien vi thiy nhilu manh mfi mem, efl nhung manh chda hat tmh bpt (1), cac manh bilu bi, bilu bl than cac te bao dai, bieu bi dudi philn la thudng mang lo khf (2). Manh mgch ridng Id hay tap trung thanh bfi (3). Long che ehd dan bao (4), spi thdnh ddy thudng xip thdnh tdng bfi (5). Tinh thi calci oxalat hinh elu gai dudng kinh 0,05-0,06 mm (6). Nhieu hgt tinh bfit hinh d u kfch thude khac nhau, nhung hat Idn dudng kinh 0,02-0,03 mm, dan le hay tdp trung thanh dam, nam rdi rde trong ede mfi (7), ede hat phan hinh trdng ddi khoang 0,03-0,04 mm (8) (hinh 5).
T ^ CHI DlTQC HQC - 03/2013 ( S 6 443 NAIVI S3)
» Nghien cifu - Ky thuat
OiOSmrn
Hinh 5: Mgt s6 dac diem bot rau ding
Hat tinh bgt (1. 7). bleu bl mang IS khi (2). manh mach (3). long che chd (4), sgi thanh day (5).
tinh the calci gxalat hlnh ciu gal (6), hat phan (8).
Hinh 6: Mgt s6 dac diem bgt rau ding bien
Manh phiin la mang Idng tiet dan bao (1). manh md mem (3). cac mach gd (4).
hat tinh bgt (5). hat phan hlnh ciu (6). long tiit (7). cic tinh thi calci oxalat (8) Rau ding bien la co cac lo khi, manh mo mem (3). Manh macti Bgt mau vang nau, mui tham, vi flang. Quan gom cac mach g6 nho rieng le hole tap tfung sat dual Isinh hi4n vi thay manh phi^n la thu&ng thSnh bo (4). H?t tinh bpt (5). Hat phin hinh cau mang nhieu long tiet dm bao (1), manh bi^u bi au-c>ng l<mh 0,02-0,025mm (6). L6ng tiet (7), cic
34 TAP CHi DirpC HQC - 03/2013 (SO 443 NAM 53)
• Nghien CIFU - Ky thuat
tinh t h i calci oxalat hinh khii kfch thude 0,005- 0,01mm (hlnh 6).
Kit qua nghien edu cho thiy rau ding va rau dang biin efl thi phan bidt d dgng duyc lipu tuai, khfl, da nghiin thdnh bfit va trong che pham sd dgng dgng bpt. Ve mgt hlnh thdi: Thdn rau dang cfi khia dpc, till chit cdng, efl bg ehia mfing. Id mpc each, philn id hpp, hoa eulng ngin mpc d ke Id. Than rau dang blln nhan, mim. Id mpc dli, philn la rpng, hoa mpc trdn 1 cuong ddi. V l vi phiu than rau ding cfi mlu loi, rau dang biin tron c6 ede khoang gian bdo. Dgc dilm bpt: Rau dang cfl Iflng che ehd, cfl eae t)6 syi, tinh thi canxi oxalat hlnh d u gai khae vfii Rau dang biin khflng cfl Iflr^ che chd, tinh thi ealei oxalat hinh khii.
Cay rau ding lau nay van duyc sd dyng trong ede bdi thuoc y hpc co truyin vdi tdn biin siic van dupc xac djnh Id Polygonum avieulare L. hp Rau ram (Polygonaeeae)'^'. Theo Thyc vdt chi Vipt Nam'"' lodi ndy duyc xdc ^nh la Polygonum plebejum R. Br.. Cin cfl them eae nghien cdu de sd dung thing nhit v l m|t thye vat va cflng dung.
Ket luan
Can cd vdo ede dae dilm hinh thdi da xde djnh mau nghidn edu Id rau ding - Polygonum avieulare L., hp Rau ram (Polygonaeeae) va rau dang blln - Bacopa monnieri (L.) Wettst., hp Hoa mom eho (Serophulariaeeae).
Cfi thi phdn bidt ede lodi rau dang d dgng tuai, khfl, dang bfit bang dde dilm hlnh thai, vi
phiu, bflt. Cac dae dilm ndy dd dupc tgp hpp thdnh bfi du lieu lam ca sd d l xdy dyng cdc chuyen lugn kilm nghidm duyc lipu.
Summary
The plants in the Vietnamese name of "Rau dang" was studied by microscpical examinationg, investigation of numerous plant samples gathered from various beations in Vietnam, on its and stating detailed description of macroscopy, morphdogy, miaoseopy (an^ombat sections and powders) and systematic taxaiomy. Also, detailed descripti(xis in these studied respects and live photos were preserved. The plants were botanically indentified as Polygonum avieulme L, and Bacopa monnieri (L) Wettst..
Keyword: Polygonum avieulare L., Bacopa monnieri (L.) Wettst., detemination.
Tai lieu tham khao 1. Vu Van Chuyen (1976), Tdm tit die diim cac ho cay thudc. NXB Y hgc, tr 39, 149, 172.
2. Pham Hoang Ho (2000), Ciy cd Viit Nam, NXB tre, quyen 1, trang 719, 738, 746.
3. Pham Hoang Hfl (2000), Ciy cd Vi$t Nam. NXB trd, quyen 2, tr912.
4. Vien khoa hpc va cflng ngh§ (2007), Thgc vit chi Viet Nam, NXB Khoa hpc vi K? thu$t, t|p 11, trang 145-147.
5. M.H. Lecomte (1941), Flore general de L'lndo - chine, lome pr^nier, pp. 263.
6. M H. Lecomte (1910), Fbre general de L'lndo - chine, tome dnqui^me, pp. 24.
•^ r
Nghien ciru dieu che fenofibrat
Trirong Phirong, Nguyen Trirdng Vi Dai hoc YDuac TP. Ho ChiMinh Oat van de
Fenofibrat Id mflt chit thufic nhfim fibrat, du'pc sd dgng phi blln trong dilu tq b^nh tdng lipid huylt, dgc bi$t la tang triglycerid vd cholesterol'^'. Fenofibrat da duyc dua vdo duyc diln mpt s l nude'^^'. Cs \flgt Nam, hi^n nay da cfi mfit s6 xf nghipp sdn xuit cdc ehl phim efl chda fenofibrat nhu SAVIPHARM,, DOMESCO, HASAN DERMAPHARM... Trong nghien edu
trudc'^', chiing tfli da nghidn edu ting hyp thdnh efing san phim trung gian 4-cloro-4'- hydroxybenzophenon. Tilp tyc nghidn edu trdn, ehiing tfll tham khdo ede phuang phdp trdn thi gidi ' ' td dfi tiln hanh nghien cdu xdy dyng qui trinh ting hyp fenofibrat td 4-cloro-4'- hydroxybenzophenon va nhdng nguyen lipu rd tiln dd kiem nhu clorofonn, aeeton, NaOH, isopropanol. San phim fenofibrat thu duyc dgt tidu ehuln Duyc diln Vipt Nam IV.
T4J> CHI DlTQC HQC - 03/2013 (S6 443 NAM 53) 35