TAP CHf KHOA HQC TRtfdNG DAI HQC T R A V I N H . S 6 26. T H A N G 6 NAM 2017
XAY DUNG QUY TRINH NHAN GIONG IN VITRO VA THUAN DUflNG GIONG CHUOI TA QUA (Musa sp.)
TAI TRA VINH
IN VTTRO BREEDING AND NURSING TAQUA BANANA IN TRA VINH PROVINCE
Dinh Thj Thanh 'ISm\ Phan Quoc Nam^, Nguyin Ngoc Trai^, Vo Van An*, Nguydn Minh Phfldng^
Tom tat - Di tdi xdy dUng vd hodn thien quy trinh nhdn giSng chuoi Td Qug bdng phuang phdp nudi cdy mo. Kit qud nghien cUu dgt dUdc nhu sau: (1) Mdi trUdng toi uu de tdi sinh choi cdy chuoi Td Qug Id mdi trUdng MS (Murashine k Skooq 1962) bd sung: 0,1 mg/l NAA, 100 mg/l adenine sulfate, 10% vA- nude dUg 30g/l saccharose, 8 g/l agar, bd sung 5 mg/l BAP vd mdu gidy dugc de trong dieu lain toi hodn toai;
mdi trUdng co 7 mg/l BAP cho kit qud dgt 6,33 choi/mdu sau 4 tudn nuoi nhdn chdi; (2) Moi trudng tot nhdt cho sU tgo re cdy chuoi Td Qug CO 1 mg/l NALA.
Ttt khoa: nuoi cay mo, chuoi Td Qua, BAP, NAA, nhdn choi chuoi.
Abstract - The goal of this study is to de- termine in vitro propagation media of Taqua banana. The results showed that: the optimal medium for banana bud regeneration was MS medium (Murashine h Skoog 1962) supple- meraed 0.1 mg/l NAA, 100 mg/l adenine sulfate, 30g/l sucrose, 8 g/l Agar, 5 mg/l BAP. 10%
volume coconut juice and kept in completely dark condition. The medium, which is similar to bud generation media except for supplementing BAP 7 mg/l, was also good for bud replication with
^Email: [email protected]
^'^'*Giang vi6n, Bo mon TrSng trot & Phat tiiio Nong thSn, Khoa Nflng nghi£p - Thuy san, Trtfdng Dai h9c Tra Vinh
^Smh vi6n, Bo mfln Tr6ng trot & Phat tri^n Nong thfln, Khoa Nflng nghiflp - Thiiy sin, TnltJng Dai hoc Tra Vinh
Ngay nhan bai: 28/10/2016; Ngay nhan kit qua binh duyS: 25/01/2017; Ngay ch^p nhan dang: 15/02/2017
6,33 shoots per sample cifter 4 weeks. (2)The medium, which is sitrdlar to bud generation media except for non-added BAP and an increase cf NAA fi-om 0.1 to 1 mg/l, was the best for the banana rooting.
Keywords: in vitro breeding, Taqua banana, BAP, NAA, banana bud regeneration
I. DATVAND^
Hien nay, nfldc ta da va dang trong rat nhilu loai cSy mang lai Idi ich cao nhfl chuoi, ehe, ca phd, bdng, day... Tuy nhidn, chuoi dfldc cho la mpt ioai cSy cd nhflng tilm nang ldn do nhflng ldi ich ma no mang lai ebo con ngfldi.
Theo Vien Nghien eflu va Phat trien Ndng nghiep Malaysia (MARDI), chuoi la loai ttai cSy duy nhSt bdi tu day du thanh phan chat dinh dfldng c4n thilt cho cd the con ngfldi. Do dd, chuoi dac biet thich hdp de bo sung khau phln dinh dfldng cho tte em va ngfldi gia.
Chuoi la cay an qua va cung la thflc pham chu yeu d nhflng nfldc dang phat trien vung nhiet dSi.
6 nfldc ta bidn nay, chuoi dfldc ttong d nhieu vung sinh thai kbac nhau tfl Bac vao Nam, tfl bai dao tdi cac vung ven bien, tfl vung trung du tdi mien niii. Chuli tai thi trfldng Viet Nam gan dSy dfldc tieu thu nhieu va cd nhilu kha nang de piiat iriin. Thy nhidn, cbuli chtfa the pbuc vu cho viec san xuat tren quy md cdng nghidp va xuat khau do hinh thflc chtfa dep, chat Ifldng chtfa cao va tt6ng nho le kbd thu hoach tap trung.
Cay chuoi Ta Qua la loai cSy thudc dang quy, cd hieu qua kinb te cao. Mdt cSy chuoi khi dtfpc ttong va cham bdn tot thi sau 8, 9 thang se ra ttai.
Gia tiitfdng lai ihu mua tai vtfdn khoang 3.000
T*P CHI KHOA HQC TRUONG D4I HOC TRA VINH, s 6 26, THANG 6 NAM 2017 NONC N G H I $ P • THUY SAN
ding/ttai. Tbeo kit qua khao sat md binh ttong xen canb tai xa Ninh Thdi, huyen Cau Ke, tinh Tta Vinh, ndng dan ed tiie tiiu tiiem tfl 6 ttieu
^ n 11 tiieu dong/lOOOm^. Iby nhien, hidn nay, md binh nay chfla dfldc nhSn rdng. Thdm vao dd, chuoi Ta Qua dfldc ttong chu ylu bang phfldng ph^ truyen thong tfle la sfl dung cay con de iroBg. Vi vay, theo tbdi gian, cay con hi thoai hoa, giong cSy nho dl bj benh va chat Ifldng ttai kem dan din ldi nhuan thap. Do dd, vide tim ra giai phap nSng cao chat Itfdng san phim, dap ling eho nhu ciu efla titi trtfdng cung nhtf dd dong deu vl Idch thtfdc cay giong, tao ra cay ttong sach benh va khdng bi thoai hda, gdp phan gia tang thu nhSp ben vflng cho ngfldi dan cbinh la nhflng yeu to flu thi cua cay giong nudi cay md.
Khi nudi cay md chudi, phan dfldc chpn de nhan giong la phiin choi non cua cay sau khi dtfde huy dinh sinh trfldng, mlu dtfpc cky vao moi trfldng tiiach cd tiianh pban dinb dfldng va chat kich thich sinh trfldng phu hdp. Cac choi ben se xukt bidn sau thdi gian tilp theo, cac choi tai sinh dfldc nhSn nhanh va tai tao cay, rl vdi so Ifldng nhfl mong muon. Vdi mdt mau ban dau se cho ra hang ngan cSy con sach benh va kich thtfde eay ding diu [1].
Nudi c^y md ttong nhan gilng cay chuoi la phfldng phap nhSn giong toi flu dl tao ra cay con giong chat Ifldng cao va dap flng du nhu clu cua tin Qiidng. Vi vay, quy ttinh nhSn gilng mpt s6 gilng chuoi cd tiem nang can dflde xSy dtfng hoan ehinh. Xuat phat tfl nhu can dd de lai: "XSy dflng quy trinh nhan giong in vitto va thuan dfldng giong chuoi Ta Qua (Musa sp.) bang phtfdng phap nudi cay md tai TYa Vinh" dfldc thtfc hidn.
H. VAT Ll£U VA PHUONG P H A P NOmfiNCtfU A. Nguyin vdt liiu
Giong chu6i Ta Qua dfldc tiiu mua va chpn lpc t^ dia phtfdng (huyen Clu Ke, tinh Tra Vinh).
Tui nilon, bdng gdn tiiam nfldc, cd chit ttong, ntfde lpc, Ifldi cbe, cao su tiang, day cbi, tte...
Dung cu: pen (nhip inox), den edn, ro nhita, xd nhtfa, mam, dao, keo, ghim kep, gang tay, khau
ttang, giSy cudn, dtfdng, giSy nhdm, tiiulc bao ve thtfe vSt.
May mdc, titiet bi: n6i Autoclave, may do pH, micropipette, ti cly, t i mat.
Hda chat: Mdi trtfdng MS chuin, chit dilu hda sinh trtfdng: BAP, NAA, adenin sulfate, myo- inositol, saccharose, tban boat tinh, agar.
B. PhUdng phdp nghiin cUu
l)M6i trUdng vd diiu kiin nudi cdy: Mdi trfldng dfldc sfl dung dl nhan choi la mdi trfldng MS dfldc bo sung: 0,1 mg/I NAA, 100 mgA ade- nine sulfate, 10% v/v nfldc dfla, 30g/l saccharose, 8gr/l agar. pH dfldc dieu chinh = 5,8. Mdi trfldng dfldc khfl trung 6 121°C, 1 attn ttong 20 phut.
Sau kbi khfl ttung, mdi trfldng dfldc rdt vao tiii nhtfa vd trung, di ngudi [2].
Cac thi nghiem dfldc dat ttong phong sinh tiitdng cd nhiet dp 26 ± 2°C dtfdi quang ky 12 gid, cung cap bang cac ong den huynh quang 1,5 m, cfldng dp chieu sang 2.000 lux.
2) Cdc thi nghiim: tbi nghiem 1. Khao sat anh hfldng cua nong dp BAP len kha nang nhSn nhanh chdi cua giong chuoi Ta Qua.
Choi con cua gilng ehudi Ta Qua sau khi dfldc tai sinh ttong mdi trtfdng MS bo sung: 0,1 mg/I NAA, 100 mg/l adenine sulfate, ntfdc dfla la 10% v/v, 30 g/1 saccharose, 8 g/1 agar, bd sung 5 mg/l BAP [3] se dtfde cly nhan nhanh ttong mdi trtfdng MS dtfde bd sung: BAP dtfde b6 sung vdi 4 mflc dp (0, 3, 5, 7 ml/1) Wdng flng vdi 4 nghiem tiiflc.
DSy la thi nghiem 1 nhan to (ehat dieu hda sinh trtfdng BAP), mdi nghiem thflc dfldc tien hanh vdi 3 lan lap lai (vdi mdi lan lap lai la mdt tui mdi trfldng cd cbfla 6 mau cay), long so ddn vi tbi nghiem la: 4 x 3 = 12.
NghlBm thiic NSng do BAPCmg/1)
Cac bpc chfla mSu cly de ttong ^eu kidn phdng nudi cac chi tieu dfldc theo ddi cl tuan thfl 4 sau khi cay gom:
T*P c m KHOA HOC TRl/dNG DAI HOC TRA VMH, SO 26. THANG 6 N A M 2017 NONG NGHlfiP - T H O Y SAN
- So choi mdi binh thanh/mlu cay - Chieu eao trung binh cua chdi (cm), so la Oa).
- Trpng Ifldng ttfdi trung binb cua cum chii (g)
Thf nghiem 2. Nghien cflu anh hfldng cua nong dd NAA va ham Ifldng khoang len kha nang tao rl, tai sinh thanh cay hoan cMnh efla tflng giong chuoi.
Mlu till nghiem la nhiing cSy cd chieu cao trung binh khoang 3,0 cm dtfpc nhan d mdi trfldng tli tfu cua thi nghiem nhSn nhanh choi.
Cac cay tfl nhflng cum choi dflde tach thanh tflng cSy ddn le.
Day la tbi nghiem 2 nhSn t l (nhSn to A: NAA vdi 4 mflc dp 0.0, 1.0, 2.0 va 3.0 mg/l; nhSn td B: ham Ifldng khoang vdi 2 mfle dd: gifl nguyen ham Ifldng da Ifldng, vi Ifldng, Fe-EDTA cua mdi tttfdng MS va giam dfi 50% bam Ifldng da Itfdng, vi Itfdng Fe-EDTA cua mdi trtfdng MS (MS/2)), 8 nghiem thflc, 3 Ian lap lai (vdi mdi lln % iai la mdt bpc nhtfa cd chfla 6 cSy cbuli dfldc cay ttdn mdi trfldng tfldng flng vdi tttng nghiem tiiflc).
TSng so ddn vi thi nghiem: 8 x 3 = 24 ddn vj thi nghidm.
N m g do NAA(mg/l)
MS NTl NT2 NT3 NT4 MS/2 NTS NT6 NT? NTS
Chi tiSu theo doi: Man ^ d c theo ddi cac ehi tidu d tuan thfl 3.
-I-1? le mlu ra rl (%): So Ifldng cay ra rl/tong so cay.
+ So Ifldng rl/cay, chieu dai re (re dai nhat tren cSy).
+ Chilu cao cay (cm) dfldc do tfl co re ldn den chdp la cao nhat.
-t- So la/cay.
m. K^T QUA VA T H A O L U A N A. Khdo sdt dnh hudng cua nong do BAP lin khd ndng nhdn nhanh choi cua giong chuoi Td Qug.
Chdi tai sinh se dtfde tach thanh tflng choi ddn le va buy ^ n h sinh trtfdng sau dd ^ d c cay vao mdi trtfdng ttfdng dng vdi tflng ngbiem thflc qua 4 tuSn ely ghi nhan so Ifldng choi dtfde nhan nhanh:
S6 chdi mdi binh thanh tang d Id thuan vdi ham Itfdng BAP dtfde bo sung vao mdi trtfdng nudi cap OBang 1). Nghiem thflc 4 (BAP 7 mg/I) cd so choi hiitii tiianh va trpng Itfdng ttfdi tnmg binh/cum la cao niiSt (lln Itfdt la 6,33 ebli/mlu ely va 1,08 g/cum choi) so vdi cac nghiem thflc edn lai. Mac du so Ifldng chii binb tbanh va trgng Itfdng ttfdi cum choi d ngbiem thfle 4 (BAP 7 mg/l) khdng cd stf khac biet y ngbia thong 1^ so vdi ngbiem thflc 3 (BAP 5 mg/l) nhflng Iai Idiac biet so vdi hai nghiem thflc cdn lai. Nghiem tiuic 1 khdng bo sung BAP nen so Ifldng choi Unh tbanh !a thlp nhlt (2,0 choi/mlu cay.)
Hinh 1: Chuoi Ta Qua d giai doan nhan choi sau 4 tuIn nudi cly trong mdi trfldng cd nong do BAP 0 mg/l; 3 mg/l; 5 mgA; 7 mg/l
Kit qua nay eung phu bdp vdi nghien cflu [4]
ttong vi nhan giong chuoi Musa sp, cho thay vdi cac nong dd BAP dfldc bo sung ttong mdi trtfdng nudi cay la 0; 2,5; 5; 7,5; 10 mg/l tiii s6 Ifldng choi hinh thanh nhilu nhat trong mdi trfldng co nong dp 7,5 mg/l BAP.
Tfl kit qua tbi nghiem ttdn, mdi tttfdng MS bd sung 0,1 mg/l NAA, 100 rag/1 adenine sulfete, 10% v/v nfldc dfla, 30gr/l saccharose, 8 g/1 agar, 7 mg/l BAP (hide chpn la mdi trtfdng de nhan nhanh gjong chudi Ta Qua - mot giong diuoi ddc dao vdi s6 Itfdng ea the cdn rlt it cl Ding bang sdng Cflu Long.
T*P CHi KHOA HOC TRUdNG DAI HOC TRA VINH, s 6 26, THANG 6 NAM 2017 NONC NCHI$P - THOY SAN
Bang 1. s d chdi mdi, chieu dai trung binh cua cum choi, s l la ttdn choi, ttpng Ifldng cum choi ehudi Ta Q u a dtfdi stf anh htfdng efla n i n g dp BAP sau tuan 4 nudi cay N ^ l m t h d c
N T l (0 mg/l BAP) NT2 (3 mg/l BAP) NT3 (S mgrt BAP) NT4 (7 mgfl BAP)
F CV (%)
So didl mA binh thanh 2,0c 4,17b 5,23ab 6,33a
• • 43,7
Chieu cao t n m g binh ciia tbM (cm)
1,07a 0.43b 0,59b 0.52b
• . 46,2
So la/cum choi
0.54a 0.36ab 0,45a 0,12c
• 53,7
TWing IiTdng tiTifi t m n g binb cum chSi (g)
0.62b 0,54b 0,98a l.OSa
.
35
Ghi chii: Trong cung mgt cot, cdc^ so co it nhdt mdt chU cdi theo sau giong nhau thi khdc biet khong CO y nglua thong ke qua phep kiem dinh LSD. ns: khong khdc biet; (*) khdc biet d mUc y nghia 5%,
(**) khdc biit y nghia d mdc 1%.
B. Nghiin cdu dnh hudng cua nong dd NAA vd hdm lugng khodng lin khd ndng tgo rd, tdi sinh thdnh cdy hodn chinh cUa tUng giong chuoi
Cac cay c l y vao mdi trtfdng vdi h a m Itfdng khoang va chat kfch tbicb sinh trtfdng khac nhau nin cho chieu dai r l , so Itfdng re cung nhfl chilu cao cSy eung khac nhau. Cac cbi tieu dtfde l l y cua hian thfl 3 sau khi cay va dfldc th^ hidn d Bang 2 d i n Bang 5 .
Bang k i t qua tin nghidm cho t h l y khdng cd stf ttfdng tac gifla N A A va h a m Ifldng khoang Ien l i le ra re cua gi6ng chuoi Ta Qua giai doan t?o r i in vitto sau 3 tuan nudi cay (Bang 2). Tl Id mlu chuli Ta Qua r a re chi chiu anh hfldng bdi NAA m a khdng chju anh htfdng bdi h a m Itfdng khoang ttong mdi trtfdng. Tl Ie m l u hinh thanh rl la cao n h l t (97,22%) khi mdi tiitdng nudi cay chfla 1 mg/l NAA, m | c du kbdng cd stf khac biet y ngMa thong ke d mflc 1% so vdi mdi trtfdng co ham Itfdng 2 mgA va 3 mg/I N A A nhflng d mflc dd 1 mg/l N A A ttong mdi trfldng van cd sfl khac biet so vdi mdi trfldng khdng b o sung N A A .
s l Ifldng rl/cSy c h u l i Ta Q u a d eac nong dp NAA khac nhau tiii cho so Itfdng r l khac nhau, nhflng lai kbdng chiu stf tac ddng cua bam Itfdng khoang Ien stf hinh thanh re cua cSy chuoi. Nong dp NAA 1 mgA cho so Ifldng re cao n h l t (2,55 rl/cay) ttong khi m d i trfldng cay khdng bd sung NAA cd so Ifldng r l trung binh/cay t h l p nhat (0,29 re/cay). Tby nghien, khi cang tang ham Itfdng N A A ttong mdi trtfdng lai lam g i ^ so Itfdng r l hinh tbanb/mlu cay cho tiily n i n g dp NAA thich h d p d^ tao r l cho cSy chudi Ta Qua la 1 mgA.
Bang 2 . So choi mdi, chieu dai trung binh cua cum chdi, s6 la tren c h i i , ttpng Itfdng cum chdi
chuoi Ta Qua dudi stf anh htfdng cua n^ng dd BAP sau 4 tuan nudi c l y Ndng d6 Ham Itfdng khoang <B) T n m g b m h (%)
NAA P MS/2
(mg/q (A) 38,89 44,44 36,11b 1 100 100 97,22a 2 83.33 88.89 91,67a 3 94.44 94,44 91,67a Trung
79,17 81,19
Ghi chu: Trong cUng mgt cdt cdc so co chU cdi theo sau giong nhau thi khdc biit khong cd i nghia thong ke qua phep kidm dinh LSD. Cdc ^ gid tri dd dugc bien ddi dudi dgng Asin^/x di xd li thong ke, cdc gid tri trin bdng Id gid tri trung binh goc. ns: khong khdc biit; (*) khdc biit d mUc y nghia 5%, (**) khdc biet d mdc y
nghia 1%.
K i t qua thi nghidm xac djnh chieu dai r l cSy chuoi Ta Qua khdng chiu sfl tac ddng cua nhan t l ham Ifldng chat khoang nhtfng chju sit tac dpng cua nong dp NAA. Trong nhflng h a m Itfdng N A A Idiac nhau se cd chilu dai rd khac nhau.
C h i l u dai r l eay c h u l i Ta Qua dat cao nhat cl
TAP CHl KHOA HOC TRUbNG DAI HOC TRA VINH. s 6 26, THANG 6 N A M 2017 N 6 N G NGHieP - T H O Y SAN
Bang 3. Anh hfldng ciia nong do NAA va ham Ifldng khoang len so Itfdng rl cua cay chuoi
Ta Qua sau 3 fiian nudi cay
Nong d o NAA (mg/l) (A)
0 1 2
3 T n m g
binh (t£/ca)>)
F(A) F(B) F(A X B)
cv
(%)
Ham Itfdng khoang (B) MS 0.23 2.6 1,67 1.5
1,5 MS/2
0,33 2,5 1,67 1.7
1,SS
"
ns ns
55,44
T n m g binh (%)
0.29c 2.55a 1,67b 1.6b
Ghi chu: Trong cOng mgt cgt cdc so cd chd cm theo sau giong nhau tfu khdc biit khdng cd y nglua thong ki qua phep kiem dinh LSD. ns:
khdng khdc biet; (*) khac biet 3 mdc y nghia 5%, (**) khdc biit d mdc y nghia 1%
Bang 4. Chieu dai re chuoi Ta Qua dtfdi tac ddng eua nong dp NAA va ham Itfdng kboang
None do STT
NAA (mgfl)(A)
1 D 2 1 3 2 4 3 T n m g binh (on/rS)
F(A) F(B) F(A X B)
CV(%)
Bam Itf<fng khoang (B) MS 0.68 2,33 1.67 [,8 1,«2
MS/2 0.96 2,32 l.S 1.93 1,7S
"
ns n . 35,23
Trung binh
(%)
0,8tc 2,33a 1,73b 1.87b
Ghi chd: Trong ciing mot cdt cdc so co chU cdi theo sau giong nhau thi khdc biit khdng co y nghia thdng ki qua phep kiem dinh LSD. ns:
khong khdc biit; (**) khdc biit d mdc y nghia 1%.
nghiem tiiflc cd ndng dd NAA 1 mg/l vdi 233 cm/rl va cd stf khac biet y nghia d mfle 1%
so vdi nghiem thflc vdi nong dd NAA 0, 2, 3 mgA. Nghiem thflc cd chieu dai r l thlp nhat la nghiem thflc kbdng co bd sung chat £eu hoa sinh trtfdng NAA vdi 0,68 cm/rl. 6 nong dp NAA 0 mgA eSy vin cd the ra re va sinh trtfdng d\i<ic nhiftig khdng tot bang cSy dtfde nudi ttong mdi trtfdng cd chfla NAA.
Bang kit qua tbi nghiem (Bang 5) eho tiily chieu cao thSn cSy va so la/cSy cua chuli Ta (Jua khdng chiu tac ddng cua bam Ifldng khoang MS ma cbl chiu stf anh htfdng cua nhSn to nong dp NAA, rieng doi vdi so la tren cay chuoi cdn chju anh hfldng cua sfl ttfdng tac gifla hai nhan to NAA va ham Itfdng MS. Chieu cao than va so la tten cSy gifla cac bam Ifldng MS khac nhau tin khdng CO sfl khac biet va chieu cao va so la cd xu hfldng giam khi nong dp NAA cang tang. Nghiem tbflc cd nong dp NAA la 0 mg/I cd chilu cao than cay va so la/tren cao nhlt vdi 5,74 cra/eay va 4,11 la/cay va khac biet y nghta thong kS so vdi cac nghidm tiiflc khac. Nghidm tbflc cd nong do NAA 2 mgA cd chieu cao than t h ^ nhat 5,26 cm/cSy va nghiem thflc NAA 3 mgA cd so la/cay tiilp nhat 3,67 la/cay.
Tbi nghiem vl anh htfdng cua nong dp NAA va ham Itfdng khoang ldn kha nang tao re, tai sinh thanh cSy boan cbinh cua chuoi Ta Qua cho thay:
De dat dtfde ti le man ra re, so Ifldng re, chieu dai xi, chieu cao tbSn va s6 la/cSy tli flu cho vide tai sinh cay chuoi Ta Qua hoan chinh, chung ta can nudi cly cSy trong mdi trtfdng cd thanh phan ctinb dtfdng: MS bo sung NAA vdi nong dp 1 mgA, 100 mgA adenine sulfate, ntfdc dfla la 10%
v/v, pH dfldc dieu cbinh = 5.8, 20grA saccharose va bo sung 8grA agar.
Ket qua nghien cflu tfldng tfl cung dfldc bao cao bdi Cronauer va Krikorian [5] kbi nghien cflu tao r l in vitto gidng chuoi Musa textihs AAA va ABB, va mdt so loai khac rang NAA (0.2-1 mg/l) de dang tao re ttong nhilu gilng chuli va nd la chit kich thich fla thich danh cho viee tao rl ciia cac giong cSy, mdi trtfdng nudi cay dtfde bd sung NAA 1 mgA la tli tfu trong viec tao re cua gilng ehuoi.
TAP CHf KHOA HOC TRtfdNG D*! HQC TRA VINH. S 6 26. THANG 6 NAM 2017
Nflng dS
B ^ 5. Anh htfdng cua nong dp NAA va ham Itfdng khoang len chieu cao tiiSn va so la cSy chuoi Ta Qua
T n m g binh
Ham Itfdng khoang (B) d i l l u cao Ham Itf^ig khoang (B) T n m g binh s o U i MS/2 OaAn^n c£y)
3,83b 3.83b NAA (mg/l)(A)
0 1 2 3
MS 5.75 5.37 5,26 5,41
MS/2 5,71 5.44 5,32 5,5
cfiy (cm
5,74a 5,4b 5,3b 5.46b T n m g binh
F(A) F (B) dliSu cao cay
F ( 8 ) s 6 l a F (A X B) chilu cao cfiy
F(A X B) s& la CV<%)
Ghi chd- Trong cUng mgt cgt cdc so cd chU cdi t
khdc biit khong cd y nghia thong ki qua phep kiim dinh LSD. ns: khdn^ khdc biet;
(*) khac biet a mdc y nghia 5%, (**) tdidc biit d mUc y n '~ ~ J sau giong nhau thi
mg khi il%.
Kk qua tten nhan tiiay rang so Itfdng rd, chilu dai rl mac du khdng chiu anh htfdng bdi ham Itfdng kboang nhtfng ttong mdi trtfdng M S ^ cae ehi so theo doi diu cao hdn so vdi trong mdi trtfdng MS. Vi vay, de mang Iai hieu qua kinh tl cho viec nhan giong cbuli Ta Qu^ bang phfldng phap nudi cay md, chung tdi chpn mdi trfldng MS^ dflde b6 sung NAA vdi nong do 1 mgA, 100 mgA adenine sulfate, 10% v/v nfldc dfla, 20grA saccharose, 8gr/l agar, pH dflde dilu chinh = 5,8 Aide chpn de tao r l cSy chuoi Ta Qua in vitro.
C. Quy trinh nhdn giong chuoi Td Qug bdng phudng phdp nudi cdy md
(WMni nJu ldn W, J pMt)
T* « * 0 « IMSJni/l BW t « WntojBl
Hinh 2: Quy ttinh nhan giong chuoi Ta Qua
rv. KET LUAN VA KI^N NGHI A. Kit ludn
Mdi trfldng thich hdp de tai sinh choi d cSy chuoi Ta Qua la mdi trfldng MS (Murashine h Skoog 1962) bd sung 0,1 mgA NAA,100 mgA adenine sulfate, ntfdc dfla la 10% v/v, 30gA saccharose, 8grA agar, 5 mgA BAP, pH 5,8 va mlu cly dfldc de ttong ^ l u kien toi hoan toiui se cho so Itfdng chii tai sinh cao nhat.
De dat sl choi nhSn nhanh tli ifli tten cSy chuoi Ta Qua, chung ta nen sfl dung mdi trtfdng cd Uianh phln: mdi trtfdng MS cd hi sung chat
TAP CHi KHOA H p c T R U 6 N G DAI HQC TRA VINH, s 6 26, THANG 6 NONG N C H I $ P - THUY SAN
dieu hda sinh trtfdng la 100 mgA adenine sulfate, 10% v/v nfldc dfla, 30 gA saccharose, 8 g/l agar, 7 mgA BAP, pH 5,8.
De dat dtfde li le mau ra re, sd Itfdng re, chieu dai re, chieu cao than va so la/ cay toi flu eho viec tai sinh cay chuoi Ta Qua boan cbinh, chung ta can nudi cay cay ttong mdi trfldng cd tiianh pban dinh dfldng: MS/2 bd sung NAA vdi nong do 1 mgA, 100 mgA adenine sulfate, 10% v/v nfldc dfla, 20grA saccharose, 8grA agar pH dfldc ^ l u cbinh d mflc 5,8 nham mang lai bieu qua kinh te cao kbi tien hanh san xuat cay giong.
B. Kiin nghi
Nghidn cflu stf ttfdng tac eua chit (£lu hoa sinh tttfdng BAP va NAA len kha nang tao r l hlnh thanh cSy hoan chinh cua cSy chudi Ta Qua.
Nghidn cflu stf ttfdng tac cua chit dilu boa sinh trtfdng BAP va NAA len kha nang tao rl binh tiianh cSy hoan chinh cua eSy ehuoi Ta Qua.
Nghidn ctfu anh htfdng cua cac tbanh phan cd chit khac ldn sinh tiirdng cua chuoi Ta Qua giai d o ^ vtfdn tfdm.
Tilp tuc nghien cflu quy trinh ttong gjong chuoi Ta Qua ngoai dong.
T A I LIEU THAM K H A O [I] T r ^ Minh Hoa, Ha Quang TtfSng, Phung Manh Hung,
Tneu Tien Dung. Ket qua hoan thien quy trinh ky thuil nhSn gi6ng va xSy diJng mS binh tham canb gi6ng chu&i xuSt khiu VNl-064. Tap chi Khoa hpc Cdng nghi. 2010;2:32I - 328. Vien Sinh hoc Nhiet d^
Vi6n Khoa hgc va Cflng nghe ViSt Nam, [2] D 6 Dang Giap, Pham Ngoc Vmh, T r k Trong Than,
Nguyen Thi Huyen Trang, Pham Ng6 Anh ThU, Thai Xuan Du Ting he so nhin choi chu6i LaBAP (Musa.sp) nufli cay in-vitro bang each s^ dung anh sang, myo-inositol va adenine sulphate. Tgp chi Sinh hoc. 2012;Vien Sinh hoc Nhiel ddft. Vien Khoa hoc va Cong ngh£ Viet Nam.
[3] Aish Muhammad, IqBAPI Hussain, S M Saqlan Naqvi, Hamid Rashid. BAPnana plantlet production through tissue culture. Agricultural Biotechnology Program (ABP) lABGR. National Agricultural Research Csto^
(NARC). Parit Road, IslamaBAPd, Pakistan2004;.
[4] Al-Amin Md, Karim M r, Amin M r, Rahman S, Maun A N M. Invitro micropropagation of Banana(Musa spp.). BAPngladesh J AgrilRes, ISSN 0258-7122.
2009;34(4):645-«59.
[S] Cronaure S S, Krikorian A D. MultipUcation of Musa from excised stem tips. Annals of Botany.
1984b;53:321 - 328. Vifin Smh hoc Nhiet ddi, Viia Khoa hoc va Cong nghe Viet Nam.