Nghien CLFU - Ky t h u a t
Tac dung ha glucose huyet cua diep ha chau dang {Phyllanthus amarus Schum et Thonn.)
tren chuot nhat trang thyc nghiem
Dat van de
Diep ha chau ding [Phyllanthus amarus Schum et Thonn) la mpt dugc lieu dugc trdng va su dung kha phd biin d Viet Nam vdi cdng dung nhu thong tieu, dieu kinh sua huylt va dac biet trong dilu trj viem gan. Cho din nay, tr§n t h i gidi da cd nhieu cdng bd v l tac dung ha glucose huylt cua diep ha chau ding tren dgng vat thi nghiem va md ra trien vgng cho viec su dung dugc lieu nay trong dilu trj dai thao dudng (DTD) '^' ®' ^\ Trong khi do, d Viet Nam, cac nghien cuu mdi chu yeu tap trung vao tac dung dieu trj viem gan, chua cd nghien cuu nao v l tac dung ha glucose huyet cua diep ha chau ding. Do dd, trong nghien cuu nay, chung tdi tap trung vao nhOng muc tieu sau:
- Danh gia tac dung ha glucose huyet cua djch chilt cay diep ha chau ding tren chugt nhit tring thi nghiem
- Lua chpn m6t liiu thich hgp cua djch chiet eay diep ha chau ding cho tac dung ha glucose huylt.
- Lua chpn phan doan dich chilt cd tac dung ha glucose huyet tdt nhit
Nguyen lieu va phu'o'ng phap nghien
CLPUNguyen lieu
- Toan cay diep ha chau ding (Phyllanthus amarus Schum et Thonn) da dugc djnh danh va luu miu tai Bd mdn Thuc vat trudng dai hgc Dugc Ha Ndi. Miu thi> dugc nua sach, phai va sly khd, tan thanh bpt th6.
- Chudt nhit tring thuin chung gidng Swiss, trpng lugng trung binh 25 g (do Vien Ve sinh djch t l cung cip) va dugc chia thanh cac Id 10 con.
Phung Thanh Htfong, Nguyen Thj Phu-ong Lan Nguyen Xuan Thang Trudng dgi hoc Duac Hd Noi
- Hoi chit: Streptozotocin (STZ) (MP Biochemicals), Adrenalin (Cdng ty CPDP TLfl) va cac hoa chit thi nghiem tinh khiet khac.
- Thiet bj: may do glucose huyet tu dpng va 06 kit thu One Touch Ultra, (Johnson &
Johnson USA), may elt quay.
Phu'ang phap nghien cu>u
Diiu chi dich chiit
- Dich chiit toin phin: chilt bing phuong phap ngam lanh dugc lieu vdi dung moi ethanol 70° trong 48 gid, lap lai 3 lan. Gop cac dich chilt, cit quay dudi ap suit giam d" nhiet dp dudi 50°C thu dugc cin. Pha hon djch cin trong nude cit vdi ty le tugng dugng Ig dugc lieu khd/ mL.
- Phan doan djch chiet: tach cac phan doan tu djch chiet toan phan bing each lie lan lugt vdi cac dung mdi cd dp phan cue khac nhau: n- hexan, cloroform, ethylacetat, n-butanol d l thu dugc 4 phan doan tuong ung va phan doan djch chilt nudc cdn lai sau cung. Cit quay dudi ap suit giam de loai dung moi thu dugc 5 ein tuang ung. Pha hdn dich c i n ciJa mdi phan doan trong nude elt tao thanh hdn djch de thCr tran chugt vdi lieu tuang duang 15 g dugc lieu khd/kg. Lieu cua tung phan doan tinh theo sd mg c i n dugc the hien trong bang 1.
Bang 1: Liiu thir nghiem tren chudt nhit (tinh theo sd mg cin) ciia cic phan do^n dich chiit
STT
1 2 3 4 5
Phan doan
n-Hexan Cloroform Ethyl acetat
n-Butanol Nudc
Hieu suit chiet phan do^n (%)
0,39 0,19 0,18 0,28 0,34
Lieu thir nghiem (mg can /kg)
59 29 27 43 52
Djnh luvng glucose huyit: phuang phap enzym glucose oxydase (GOD) vdi may do t u dpng va que thu
Nghien ciru tac dung ha glucose huyit tren chugt binh thir&ng
- Chupt nhjn ddi 12 gia duge cho udng djch chilt toan phin vdi liiu 0,5g ein/kg tuong duong lOg duoc lieu khd/ kg. Dinh lugng glucose huylt mdi gio mpt l l n trong vong 4 gia. T i l n hanh song song vai 16 chung udng nude cit.
- Thi nghiem dugc tiln hanh tuong t u vdi 5 Id udng phan doan djch ehilt vdi liiu tuong duong 15 g/kg. So sanh vdi 16 chung udng nudc e l t
Nghien ciru tac dung ha glucose huyit tren chugt tiem STZ
Sau khi tiem mang bung STZ (150mg/kg) 72 gia, djnh lugng glucose h u y l t luc ddi cua chupt.
Lua chpn chupt cd glucose huylt tren 12 mmol/L. Sau dd chia chupt thanh cae 16. Vdi 16 thu, eho udng dich chilt toan p h i n vdi l i i u tuong duong 10g/kg/ngay trong vdng 10 ngay lien tue. T i l n hanh song song vdi 16 chung udng nudc c it.
- Thi nghiem dugc t i l n hdnh tuong tu vdi 5 16 udng phan doan dieh ehilt vdi l i i u tuong duong 15 g/kg/ngdy. So sanh vdi 16 chung udng nudc e l t
Nghien ciru tac dung ha glucose huyit tren chugt tiem adrenalin
- Chudt nhjn ddi 12 gid dugc cho udng djch chilt (liiu tuong duong 10 g duac lieu khd/kg).
Sau 3 gid, tiem mang bung adrenalin vdi liiu 0,6 mg/kg, djnh lugng glucose huylt vao cac thai diem trudc khi tiem va sau khi tiem 60 phut. T i l n hanh song song vdi 16 chung udng nudc elt.
- Thi nghiem duoc lap lai vdi cac lieu dieh chilt khdc nhau: 5g/kg, 10 g/kg, 15 g/kg, 20 g/kg. So sanh mue tang glucose huylt sau khi tiem adrenalin cua cdc 16 chuot, t u dd lua chpn mpt liiu dung hieu qua nhit.
Xir ly s6 lieu
Bllu d i l n sd lieu dudi dang Xtb ± SD. Sd lieu dugc x u ly b i n g phuang phap thdng ke sinh hpc vdi Test Student va s u hd trg bdi p h i n m i m EXCEL 2003. Su khac biet cd y nghia thdng ke khi p < 0,05.
K§t qua nghien
CLPUAnh hir&ng cua djch chiit toan phan tren glucose huyit cua chugt binh thuxyng
Bang 2: Muc glucose huyit tren chudt binh thudng udng djch chiit
Glucose huyet p(a ± SD) (mmol/L)
2 gid' 3 gia 4 gia
Chimg 6,38 ± 0,21 5,93 ± 0,16 5,90 ± 0,23 5,87 ± 0,17 p> 0,05* p > 0,05* p > 0,05*
DHCtfang 6,20 ±0,15 5,66 ±0,21 5,12 ±0,21 4,89 ± 0,20 p<0,05* p<0,05* p< 0,001*
(*): So sinh vdi glucose huyet thai diem 0 gid;
Nhan xit: & 16 chung, glucose huylt thay ddi khdng dang ke theo thai gian (p > 0,05), trong khi dd d 16 thCr, t u sau 2 gid trd di, glucose huylt b i t d i u giam. Dae biet sau 4 gid, glucose huylt giam manh n h i t (21,13%), khac biet rd ret so vdi ban d i u (p < 0,001).
Anh hu'd'ng ciia djch chilt toan phin tren glucose huylt cua chuot tiem adrenalin
Bang 3: Muc ting glucose huyit sau khi tiem adrenalin
Glucose huyet (Xtb±SD)
L6 (mmol/L) 0 phiit 60 phut
MU'C tang
9 ' " ^ ° f pso vd'i
huvet(X„±SD) '°'^^'^"3 (%)
Chu'ng 6,43 ± 0,34
13,99 ± 0,99
126,35 ± 7,49 DHC
dang
6,28 ± 0,30
12,47 ± 0,94
99,61 ±
5,30 p<0,05
Nhin xit:
- Sau khi tiem adrenalin l i i u 0,6mg/kg, glucose huyet cua chugt d 2 16 d i u tang, nhung d 16 udng DHC d i n g , muc tang glucose huylt (99,61%) t h i p hon cd y nghTa thdng ke (p<0,05) so vdi Id chung (126,35 %).
Nhu vay, dich ehilt DHC d i n g (liiu lOg duoc lieu khd/kg) co tac dung han c h i tang glucose huylt tren chupt tiem adrenalin.
Anh hu'dng cua djch c h i l t toan p h i n tren glucose huylt ciia chuot tiem STZ
Bang 4: Mirc ha glucose huyit cua chudt sau khi tiem STZ
Glucose huylt "Vluc ha , . (X,b + SD) (mmol/L) g|"cose Lo huyet ,. , ,
NgayO Ngay 10 (^tb + bU)
^ '^ ^ ^ (%) Chung 20,44 ± 21,69 ± , -3,24 ±
" 2,16 1,43 0,56 r>u/--»4„„ 16,87± 15,00± 23,41 ± ^n nn^
DHCdang ^^^^ ^ p<0,001
TAP CHi DUQC HQC - 1/2010 (SO 405 NAM 50) 31
Nghien CLFU - Ky thuat
Nhan xit:
- Sau 10 ngay udng djch chilt DHC ding (lOg dugc lieu khd/kg), glucose huylt cua chu6t giam ro ret (23,41 %). Trong khi dd d 16 chung, glucose huylt tiep tuc tang len. Muc ha glucose huylt d 16 chupt udng DHC ding khac biet ro ret so vdi 16 chung (p<0,001).
Nhu vay, djch chilt DHC ding (lOg dugc lieu khd/kg) co tac dung ha glucose huylt tren chupt tang glucose huylt bing STZ sau 10 ngdy dilu trj lien tuc.
SiF phu thupc liiu lu'o'ng cua tac dung ha glucose huylt cua djch chilt toan phin
Sau khi khing djnh tac dung ha glucose huyet cua djch chilt toan phin DHC ding vdi liiu 10 g/kg, chung tdi tiep tuc tim hieu su phu thugc lieu lugng cua tac dung ha glucose huylt nhim lua chgn mgt lieu dung thich hgp cho cac thi nghiem tiep theo. Thi nghiem nay dugc thuc hien tren chugt tang glucose huylt thuc nghiem bdi adrenalin. Chugt dugc cho udng cac liiu dich chilt khac nhau va so sanh muc tang glucose huylt cao nhit giua cac Id.
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^
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D . C 0)
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cn cn
•ni r u
2 0 0 1 8 0
l e o
1 4 0 1 2 0 I O O 8 0 S O AO
2 0 O
-X^
p < 0 , 0 1 *p > 0,05**
2 L o 3
(5/kg) (10 g/kg) (15 g/kg) (20 g/kg)
Hinh 1: Muc tang glucose huyit cua chudt tiem adrenalin udng dc liiu djch chiit khac nhau (*): so sinh vdi Id 2, (**): so sinh Id 3 vdi Id 4
Nhan xet:
Cd mdi tuong quan ti le thuan gilj'a liiu dung va tac dung han ehi tang glucose huylt cua DHC ding, khi liiu udng djch chilt DHC ding cang eao thi mue tang glucose huylt cang giam. Muc tang glucose huylt thip nhit d liiu 20g/kg, tuy nhien muc tang glucose huylt giua
liiu 15g/kg va 20g/kg khac biet khong ed y nghTa thdng ke (p>0,05). Do vay, liiu 15g/kg dugc xac djnh la liiu dung hieu qua nhit de dung cho cac nghien cuu tiip theo v l tac dung ha glucose huylt cua DHC ding.
Tac dung ha glucose huylt tren chuot binh thu'd'ng cua cac phan doan djch chilt Bang 5: Anh huang cua cic phan doan dich chiit DHC ding tren glucose huyit cua chudt blnh thudng
Lo ChUng Pd n-hexan Pd chloroform Pd ethyl acetate
Pd n-butanol Pd nudc
Glucose h u y l t (X,^
Oh 6,23 ± 0 , 1 5 6,31 ± 0 , 1 8 6,20 ± 0 , 1 3 6,38 ±0,21 6,51 ±0,29 6,43 ±0,26
± SD) (mmol/L) 4h 5,61 ± 0 , 2 3 5,73 ± 0 , 2 2 5,45 ± 0 , 1 9 4,08 ±0,25 5,52 ±0,45 5,46 ±0,32
Muc ha glucose huyet
(X„±SD)(%)
9,43 ± 2,49 9,12 ± 2 , 6 1 14,57 ± 2 , 2 0
42,74±2,97 16,79±0,90 15,32 ± 3 , 2 8
p so v6i Id chung
p > 0,05 p < 0,01
p > 0,05
Nhan xet:
- 0 cac Id udng phan doan n-hexan, cloroform, butanol, nudc, muc ha glucose huylt cua chupt khac biet kh6ng co y nghTa so vdi 16 chung (p>0,05).
- Lo udng phan doan ethyl acetat cd muc ha
glucose huyet nhilu nhit va khac biet cd y
nghTa so vdi 16 chung (p<0,01). Nhu vay trong
t i t ca cac phan doan djch chiet cua DHC
ding, chi djch chiet phan doan ethyl acetat ed
tac dung ha glucose huyet tren chugt binh
thudng.
Tac dung ha glucose huylt tren chuot tiem STZ cua cac phan doan djch chilt
Bang 6: Anh hudng cua cic phin doan djch chiit DHC ding trdn glucose huyit cua chudt tiem STZ
L6
Chung n-hexan Cloroform Ethyl acetat
Butanol Phan doan nu'dc
Glucose huyet (X,,, Ngay 1
20,47±1,39 20,65 ± 1 , 7 3 20,23 ± 1 , 6 0 19,91±2,02 21,17±1,78 21,20 ± 1 , 7 4
±SD) (mmol/L) Ngay 10 19,65±1,71 15,21 ±1,71 17,65 ±0,86 14,53±2,15 19,62±1,61 19,07+1,96
MU'C ha glucose huyet
(X„±SD)(%)
5,68 ± 1,28 28,40 ±6,41 16,50 ± 5 , 8 4 28,91±4,15
8,09±0,81 12,87 ±3,28
p so von 16 chirng
p < 0,05 p > 0,05 p < 0 , 0 5 p > 0 05
Nhin xet:
Sau 10 ngay d i l u trj lien tue vol cdc phan doan dich chilt DHC d i n g , mue giam glucose huylt d cae 16 ehu6t udng phan doan n-hexan (28,40 %) va phan doan ethyl acetat (28,91%) khdc biet cd y nghTa thdng ke so vdi 16 chung (5,68 %) (p<0,05).
(y cdc 16 udng phan doan cloroform, butanol, ,nuac mue ha glucose huylt khac biet khdng ed
y nghTa so vdi 16 chung (p>0,05).
Ban luan
v l tac dung ha glucose huylt cua djch chilt toan phin
K i t qua trong bang 1 eho t h i y dieh ehilt todn phin DHC d i n g ed tdc dung ha glucose huylt tren chupt binh thuang. K i t qua ndy tuong tu vdi edng bd cua Moshi (1997) khi nghien cuu v l anh hudng eua DHC d i n g tren thd binh thudng va eua Adeneye khi nghien cuu tren chudt n h i t t r i n g binh thudng '^' °'.
Tren md hinh tang glucose huylt thuc nghiem b i n g adrenalin l i i u 0,6mg/kg, glucose huylt eua chupt tang dang ke song mue tang glucose huylt cua 16 udng dich ehilt DHC d i n g (99,61%) t h i p han ro ret (p<0,05) so vdi mue tang glucose huylt eua 16 chung (126,35%).
Nhu vay djch chilt DHC d i n g ed tdc dung han c h i tang glucose huylt do tiem adrenalin. X u i t phat tu CO e h i gay tang glucose huylt cua adrenalin, ed the dua ra gia thilt DHC d i n g ed tac dung uc c h i s u phan huy glycogen thanh glucose, tang cudng tdng hgp glycogen du trij va uc e h i tan tao glucose d gan va co.
De tim hieu ro hon v l tac dung kich thich tuy tdng hop va bai tilt insulin cua DHC d i n g , chung tdi da tiln hanh nghien cuu anh hudng cua dich chiet DHC d i n g tren md hinh gay DTD bing STZ. STZ la m6t thudc chdng ung thu, cd kha nang pha huy t l bao beta tuyln tuy. Su pha huy
TAP CHi DUQC HQC - 1/2010 (SO 405 NAM 50)
nay d i n d i n su thilu hut tuang ddi hay tuyet ddi insulin, tuy theo mue dd ma gay nen su tang glucose huylt, vi vay STZ duac su dung de gay md hinh DTD tren dpng vat thue nghiem. Nhilu nghien cuu da chung minh ndng dd glucose huylt chi tang khi 80% t l bao beta bj pha huy vi muc dp pha huy phu thudc vao liiu tiem STZ.
Trong nghien cuu nay chung toi s u dung liiu STZ 150mg/kg. Theo nhan dinh cua nhilu tac gia nhu Dao Van Phan (2000) '^', Ta Thanh Van (2006) '^' tiem STZ liiu 150 mg/kg tren chudt nhit tring lam pha huy kh6ng hoan toan tieu dao tuy, gay duge tinh trang glucose huylt tang cao va dn djnh trong nhilu ngay. Cae dieh ehilt dugc thu tac dung tren mo hinh nay cd the gay ha glucose huyet do tac dpng true tiip len dao tuy hay ea e h i ngoai tuy khdc. Do thude ed ngudn gdc duac lieu, thudng gay tac dung cham va lau dai nen trong nghien cuu nay chung toi da cho chupt udng thude trong nhilu ngay de danh gia tac dung d i l u tri keo dai eua DHC d i n g . K i t qua cho thiy, sau 10 ngay lien tuc udng dich chilt, glucose huylt cua chupt ha xudng dang ke (23,41%) trong khi dd glucose huylt cua Id chung khong giam tham ehi cdn tang len so vdi ban d i u . Nhu vay djch chilt DHC d i n g ed tac dung ha glucose huylt tren chupt gay DTD bing STZ. Khi nghien cuu tac dung ha glucose huylt cua DHC d i n g tren chupt gay DTD b i n g alloxan, Raphael (2002) cung cho k i t qua tuang tu. Dich chilt methanol eua DHC d i n g lam ha glucose huylt (18,7%) tren chupt gay DTD b i n g alloxan sau 4h udng dich chilt liiu lOOOmg/kg. 15 ngay lien tuc udng djch chilt DHC d i n g cd kha nang giam dang k l glucose huylt cua chudt (p<0,001), va sau 18 ngay dieu tn b i n g DHC ding ndng dp glucose huylt cua chupt tiem alloxan g i n nhu trd v l muc binh thudng '°'. Nhu vay, vdi liiu STZ 150mg/kg, khi t l bao beta tieu dao tuy chua bj pha huy hoan toan, cac hoat
33
Nghien CLFU - Ky thuat
chat trong djch chilt DHC d i n g cd the phat huy tac dung kich thich sd t l bao beta cdn nguyen ven tang cuong bai tilt insulin va d i n d i n ha glucose huylt cua 16 chupt udng djch chilt DHC ding. Tuy nhien cung khong the loai tru kha nang cac hoat c h i t trong djch chilt cd tac dung lam tang tinh nhay cam cua cac receptor vdi insulin.
v l mdi lien quan liiu lu'gng - tac dung ha glucose huylt cua djch chilt toan p h i n
Sau khi da k h i n g djnh tac dung ha glucose huylt cua djch chilt DHC d i n g , chung toi t i l n hanh tim hieu s u phu thupc l i i u luang - tac dung eua djch chilt toan p h i n vdi 4 l i i u dich chilt khac nhau. K i t qua eho t h i y vdi khoang lieu lua chpn cho thi nghiem nay, cd s u phu thupc giO'a tac dung ha glucose h u y l t vdi l i i u lugng djch chilt s u dung. L i i u dich c h i l t tuong duong 15 g dugc lieu khd /kg dugc lua chon de dung cho cdc thi nghiem t l l p theo v l tac dung ha glucose huylt cua DHC d i n g .
v l tac dung ha glucose huylt cua cac phan doan djch chilt
De t i i p can g i n hon vdi viec tim hieu co che tac dung ha glucose huyet eua DHC d i n g , chung tdi da t i l n hanh phan doan djch ehilt DHC d i n g b i n g cac dung mdi cd dp phan cue khac nhau va nghien cuu anh hudng cua cac phan doan tren glucose huylt cua chupt binh thudng va ehu6t gay DTD b i n g STZ (150mg/kg). K i t qua eho thiy, tren chupt binh thuang ehi ed phan doan ethyl acetat gay ha glucose huylt, muc ha glucose h u y l t la dang ke (42,74%) so vOi 16 chung (p<0,001) va eac phan doan cdn lai. Nhu vay, eac hoat c h i t trong phan doan ethyl acetat ed the la nhan td chinh gay tac dung ha glucose huyet tren chupt binh thudng cua djch c h i l t DHC d i n g , thdng qua tac dung len t l bao beta tieu dao tuy gay tang tdng hgp va t i l t insulin, k i t qua la lam ha glucose huylt cua chupt binh thuang, cung co t h i tac dung ha glucose h u y l t nay cdn do s u phdi hgp cua cac ea e h i ngoai tuy.
Tren chupt gay DTD b i n g STZ l i i u 150mg/kg, sau 10 ngay udng djch chiet, chi cd 16 chupt udng phan doan djch chiet n-hexan va phan doan ethyl acetat la cd muc ha glucose h u y l t dang ke so vdi 16 chung (p<0,05). Tuy nhien kh6ng cd s u khac biet giCfa muc ha glucose huylt d 16 n-hexan va 16 ethyl acetat.
Nhu vay, r i t eo the djch c h i l t n-hexan khdng co tac dung ha glucose h u y l t tren chupt binh
thudng ma chf cd tac dung ha glucose huylt tren chugt cd glucose huyet tang cao. Neu djch c h i l t cd kha nang kich thich tdng hgp va bai tilt insulin thi se gay ha glucose h u y l t tren ca chugt binh thudng vi chugt glucose huyet tang cao. Nhu vay, djch c h i l t n-hexan gay ha glucose huyet theo c a che ngoai tuy, vi du nhu uc c h i s u phan huy glycogen thanh glucose, tang tdng hgp glycogen d u tru', tang tan tao glucose, tang s u dung glucose ngoai vi ....
Trong khi dd, phan doan djch chiet ethyl acetat cd tac dung ha glucose h u y l t tren ca chudt binh thudng va chupt ed glucose h u y l t tang cao.
D i l u dd eho t h i y phan doan dich c h i l t ethyl acetat cd the kich thich t u y l n tuy tdng hgp va bai t i l t insulin hoac tang tinh nhay cam eua receptor insulin d t l bao dich, hoac phdi hgp cua nhilu c o c h i ngoai tuy.
Flavonoid la thanh p h i n hoat c h i t thuong hien dien trong phan doan ethyl acetat va day cd t h i la nhan td tao nen tac dung ha glucose h u y l t cua djch c h i l t ethyl acetat Mdt sd flavonoid d u g c cho la cd kha nang ha glucose huyet nhu mtin, isoquercitrin '^' ^. NhO'ng flavonoid d u g c xac djnh trong thanh p h i n hoa hpc ciJa DHC d i n g la rutin, isoquercitrin quercetin.
quercitrin. [4] Dieu dd cho phep chung tdi dua ra gia thiet rutin, isoquercitrin cd the la hoat chat chinh gay ha glucose h u y l t cua phan doan djch chiet ethyl acetat ndi rieng va DHC ding ndi chung.
K§t luan
v l tac dung ha glucose h u y l t cua djch chilt toan p h i n :
- Djch c h i l t DHC d i n g lieu lOg/kg cd tac dung ha glucose h u y l t cua chugt binh thudng.
- Djch c h i l t DHC d i n g lieu lOg/kg cd tac dung han c h i s u tang glucose huylt do adrenalin va STZ.
L i i u thich hgp cho tac dung ha glucose huylt cua djch chiet toan p h i n DHC d i n g la lieu tuang duang 15 g d u g c lieu kho/kg
Ve tac dung ha glucose h u y l t cua cac phan doan dich chilt:
- Phan doan ethyl acetat cd tac dung ha glucose huyet tren chugt binh thudng.
- Phan doan n-hexan va phan doan ethyl acetat cd tac dung ha glucose h u y l t tren chupt gay tang glucose huyet thuc nghiem b i n g STZ.
Summary
Hypoglycemic activity of Phyllanthus amarus Schum et Thonn was studied. The extracts had hypoglycemic activity on normoglycemic mice and mice with adrenalin- and streptozocin- Induced hyperglycemia. The suitable dose of Phyllanthus amarus extract for hypoglycemic activity In mice was 15g dried plant/kg. Of various fractions of the ethanol extract, ethyl- acetate fractions had hypoglycemic activity on normoglycemic mice and streptozocin-lnjected mice, while n-hexan fraction had hypoglycemic activity on streptozocin-lnjected mice only.
Key words: Phyllanthus amarus, hypoglycemic
activity
Tai lieu tham khao
1. Ta Thanh Van, Nguyen Phuong Thuy, Khao sat tac dung hg dudng huyet cua djch chiet cay dua can {Catharanthus roseus) tren chupt nhat trang binh thudng va chupt gay dai thao dudng b i n g Streptozotocin, Tap chi Y hpc Vi$t Nam, so 3 t r i 5-20, (2006).
2. Nguyen Ngoc Xuan, Dao Van Phan, Nguyen Duy Thuan, Budc dau tim hieu co' che tac dyng ha
glucose h u y l t cua thd phye linh (Smilax glabra Roxb- Lillaceae), T^p chi nghien ciru y hoc, 11 (1), tr 37-42, (2000).
3. Adeneye AA, Adeneye AK, Hypoglycemic and hypocholesterolemic activities of the aqueous leaf and seed extract of Phyllanthus amarus in mice, Fitoterapia, 77(7-8), pp 511-4, (2006).
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Nghien ciru tinh an toan cua djch chiet te bao
r
sinh k\io\ sam Ngoc Linh tren thyc nghiem
Dat van de
sam Ngpc Linh, hay sam Viet Nam (Panax vietnamensis) \a loai cay thudc qui, cd thanh phin hda hgc va tac dung dugc ly tuong tu nhu sam Trilu Tien va cac cay thude trong hp nhan sam '^'. Oe phat then ngudn duge lieu qui hilm nay, Hpc vien Quan y da hgp tac vdi Han Qudc phat then ky thuat sinh khdi t l bao sam Ngoc Linh. Sinh khdi t l bao sam Ngoc Linh da dugc xac dinh thanh phin hda hgc gidng vol sam Ngpc Linh tu nhien, chit lugng dn dinh khdng phu thu6e vao dilu kien thdi tilt va mdi trud'ng ben ngoai '^"l Trudc khi nghien cuu tac dung sinh hgc cua c h i phim, chung toi tiln
Nguyen Van Long, Nguyen Hoang Ngan Vu Binh Du-ong, Nguyen Van Minh Hoc vien Qudn y
hanh nghien cuu danh gia tinh an toan cua djch chilt sinh khdi t l bao sam Ngpc Linh.
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