Tai li|u tham khio
1. Nguyen M. D., Nguyen T. N., Kasai R-, Ito A., Yamasaki K., Tanaka O. (1993), Saponins from Viemamese ginseng, Panax vietnamensis Ha et Grushv. Collected in central Vietnam. 1, Chemical & Pharmaceutical Bulletin, 41, 2010-2014.2, Nguyen M. D., Kasai R., Ohtani K., Ito A., Nguyen T. N., Yamasaki K., Tanaka O. (1994), Saponins from Vietnamese Givistng, Panax vietnamensis \\^e,tGmsh\. Collected in central Vietnam. II, Chemical & Pharmaceutical Bulletin, 41,115- 122. 3. Nguyen M., Kasai R., Ohtani K., Ito A., Nham N. T., Yamasaki K., Tanaka O. (1994), Sq)onins from Vietnamese ginseng, Panax vietnamensis Ha et Grushv. collected in central Vietnam. Ill, Chemical & Pharmaceutical Bulletin, 42,634- 640. 4. HujTih Thj Nggc Ngin, Tnrong Vu Hoii Thu, Hi Dieu Ly, Duong Hong To Quyen, Nguyin Minh DiJc (2013), Thiet lap chat chuin ginsenosid-Rb] va ginscnosid-Rgj, Tap chi Duac lieu, Sd 4, 258-266. 5. L6 Thj Hdng VSn, L6 Thj Mai Suong, Nguyen Nggc Khoi, Nguyen Dure Tuan, Ducmg Hong Td Quyen, HiDi^u Ly, Nguyen Minh Dfrc (2013) Phan ISpvi thi^ 1^ chit chuin maJonosid-R2 tii Sam Vi^t nam (Panax vietnamensis Ha et Grashv.), Tgp chi Du(fc lieu, 18(8), 14-20 6.
Nguyen Minh Due (1994), Chemical study on the saponin composition of Vietnamese Ginseng, Doctoral Thesis, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 18. 7. Bg Y td, Dugc dien Vi?t Nam IV (2009), Nxb Y hgc Hi Ngi, 879-880. 8. ISO 13528:2005 (2005), Statistical methods for use in proficiency testing by interiaboratory comparisons. 9. ISO Guide 34:2009 (2009), General requirements for the competence of reference material producers. 10.
ISO Guide 35:2006 (2006), Reference materials - General and statistical principles for certification.
Tap chiDuac li^u, tSp 19, sd 6/2014 (Trang 358 -362)
B l / d C D A U K H A O S A T T H A N H P H A N H O A H O C T R A I Q U A C H 6 T R A V I N H
Ngo Quoc Luan'' *,LS Thi Hong Phufng', Bang Huynh Giiip' va Ngo Khdc Khang Minh' 'Bgi hoc Can Tha; 'Bai hoc Bdch khoa - Bgi hpc Quoc gig TP.HCM
*Email: [email protected] (Nhjn bU ngay 09 thing 10 nam 2014)
Tdm tat
Nghien cuu t h ^ h p h k h6a h(?c ciia cao chiet ethanol tir tMi qudch dupe thu hSi tai tinh Tr^ Vinh, 4 hpp ch^t \k ^ sitosterol (1), daucosteroi (2), lupeol (3) v^ n-buthyl-0-^-D-fructopyranosid (4) da phan lap v^ xac djnh elu tme dua Uen c&:
phuong phip phS nghiem ('H-NMR, "C-NMR, DEPT, HSQC, HMBC vS ESI-MS). D3y IJ cong b6 dSn tien ve su ei m}t eiia chat so 4 trong lo Ji i, acldissima.
Iix kh6a; Lmonia acidissima. Lupeol. n-Buthyl-0-)i.D-fnictopyranosid.
Summary
Preliminary Chemical Investigation on the FruU ofLlmonia acidissima L. in Tra Vinh Province Phytoehemical study on the ethanol extract of fruit of Lmonia acidissima collected in Tra Vinh province resulted in the isolation of four compounds, including ^-sitosterol (1), daucosteroi (2), lupeol (3) and n-buthyl-O-^-D-fructopyranoside (4).
The structures of these compounds have been elucidated by spectral analyses ('H-NMR, "C-NMR, DEPT, HSQC, HMBC, and ESI-MS). This is the first report on the presence of compound 4 in the plant i, acidissima.
Keywords: Limonia acidissima. Lupeol. ii-Buihyl-0-/l-D'fructopyranoside.
1. G i o i t h i e u n g u o i ta con c o y t u o n g ch6 bi6n nir6c trai quach Cay quach, con goi la can thang, c o ten khoa len men [2].
hpc la Limonia acidissigma L. h p cam (Rutaceae), Tren the gidi d a c o m p t so c o n g trinh nghiSn d u p e trong nhiSu a c a c tinh T r a V i n h , Ben T r e , ciru v6 hoat tinh sinh hpc va t h a n h phan hoa hpc Soc TrSng. Theo dan gian, trai quach chin dupe cua trai quach tir nam 1986 va da cong bo phta diing nhu rapt mon giai l<hat co huong vj thom lap dugc khoang 25 hpp chjt [3-6]. 6 ViptNam, ngon dac bist hay ngam rugu uong co tac dung day la ISn dau tien chiing toi nghien ciru thinh bo than va cuong gan cot [1]. Ngoai ra, gan day phan hoa hpc va buac dju da phan Ijp dupe 4 358 Tgp chiDuac lieu, tap 19, so 6/2014
hgp chat tu trai quach, trong dd cd 1 hgp chit mdi phat hifn trong loai.
2. Nguyen lieu va phinmg phap thyc nghiem 2.1. Nguyen lieu
Trai quach dugc thu hai tai huyfn Clu Ke, tinh Trd Vinh. Ten khoa hgc dugc ThS. Dang Minh Quan - Tnrcmg Dai hgc Cin Tho giam djnh bdng phuong phap so sdnh hinh thai. Todn bg trai qudch chat lugng tot dugc tach ra, sly d 50 'C d^n dg am cdn dudi 2%, xay thanh bgt ldm nguySn lif u nghien cdu thanh phan hda hgc.
2.2. Phmmgphdp chiet xudt, phdn Igp Chiet ran-ldng, Idng-ldng, soxhlet bang cde dung mdi cdn 96", n-hexan (H), cloroform (C), ethyl acetat (E), n-buthanol (B), methanol (M).
Cat thu hoi dung mdi bang may cd quay dudi ap suit gidm (Buchi R-210). Sde ky cgt pha thudng (CPCC) vdi kich cd cgt tuy theo lugng mdu. Chit hip phy pha thudng Id silica gel cd hat 0,040- 0,063 mm (240-400 mesh) cua cdc hang Himedia, Scharlau, Merck. Pha dgng Id cac dung mdi H, C, E va M ciia VN-Chemsol.
2.3. Phuang phdp phdt hi?n va n/idn danh chdi Sdc k^ ldp mdng (TLC) su dyng ban nhdm silica gel 6OF254 (Merck) trdng san dg day 0,2 mm. Phat hi^n vet bang den tu ngoai hai budc sdng 254 va 365 nm, ho$c phun dung djch H2SO4 10% trong EtOH va ha ndng tren bep di?n. Xdc djnh cau true va nh^n danh chat dga vao cde phuang phap phd nghi?m nhu 'H-NMR, '"^C- NMR, DEPT, HSQC, HMBC, ESI-MS. Diem chdy (mp.) dugc do tren may Electrothermal 9100 (U.K), dung mao qudn khdng hieu ehinh.
PhS NMR dugc ghi tren mdy Bruker AM500 FT- NMR Spectrometer. Phd khdi lugng dugc do tren mdy HPI 100 series LC/MSD Trap, Agilent tai Vi^n Hoa hgc - Vi?n Han Lam Khoa hgc vd Cdng Ngh^ Vi^t Nam.
2.4 Qud trinh phdn Igp cdc h(rp chdt Bgt d-di quach (1,8 kg) duge chiet xudt vdi ethanol 96% bdng phuang phdp ngam lanh, Igc bd ba, phan djch chiet dugc cd dung mdi dudi dp suit gidm, thu dugc cao EtOH khoi iugng la 150 g. Sau dd, chiet tir pha rdn lln lugt vdi cdc dung mdi cd dg phan c^rc tdng din H, E vd B. Cat thu h6i dung mdi dudi dp suit giam thu dugc cdc cao
chiit phan dogn tuong img la FH (12,4 g), FE (32,7 g) va FB (25,5 g).
Thyc hien CPCC eao FE (30 g) tren silica gel pha thudng vdi he dung mdi H:E vdi ty le tang din dg phan eye 0 - 100% thu 8 phan doan (FEl- FE8). Phan doan FEl (0,3 g) ti€p tyc tien banh CPCC vdi he H:E 98:2 thu dugc 5 phan doan (FE1.1-FE1.5). Phdn doan FE1.2 cho k6t tinh lai trong n-hexan thu dugc hgp chat 1(11 mg). Phan doan FEl .4 (90 mg) tiep tuc tdch bing CPCC he C:M 95:5 thu dugc 3 phdn dogn (FEl.4.1- FE1-.4.3). Phan doan FEl .4.2 cho kit tinh lai trong
«-hexan thu dugc hgp chat 3(15 mg).
Phan doan FE2 (0,2 g) tiep tuc thue hien CPCC he H:E tu 9:1 din 5:5 thu dugc 5 phan doan (FE2.1-FE2.5). Phan doan FE2.3 (80 mg) lai tiep tyc tach bdng CPCC h? C:M 95:5 thu dugc 4 phan doan (FE2.3.1-FE2.3.4). Phan doan FE2.3.2 cho kit tinh lai trong MeOH thu dugc hgp chdt 2 (20 mg).
Tir cao FB (25 g), tiln hanh CPCC vdi EtOAc 100% thu dugc 6 phan dogn (FBI-FB6), phdn doan FB3 (3,37 g) tiep tyt; CPCC vdi h? CHCI3 100% thu duge 5 phan doan (FB3.I-FB3.5). Phan doan FB3.2 (1,86 g) lai tilp tyc CPCC vdi h? C:M 9:1 thu dugc 3 phan doan (FB3.2.1-FB3.2.3), phdn doan FB3.2.2 (51 mg) cho kit tinh lai trong CHCI3 thu dugc hgp chat 4 (15 mg).
2 J Dgc diem vgilyvdsd li^u phdn tich pho nghidm (1): Bgt mau trdng, mp. 136-137 °C. ESI-MS:
m/z 415 [M+U]+. 'H-NMR (CDCI3, 500 MHz, 8H ppm, J Hz): 0,70 (3H, s, H-18); 0,84 (3H, s, H- 29); 0,92 (6H, s, H-26, 27); 0,95 (3H, s, H-21);
1,03 (3H, 5, H-19); ... 3,55 (IH, (/, 7 = 5,0; H-3) va 5,37 (IH, ^, y = 4,5; H-6). '^C-NMR (CDCI3, 125 MHz, 6c ppm): 12,65 (C-18); 19,19 (C-29);
19,45 (C-19); 19,80 (C-21); 20,22 (C-26); 21,50 (C-27); 23,49 (C-11); 24,71 (C-28); 26,51 (C- 15); 28,65 (C-23); 29,58 (C-16); 31,31 (C-25);
32,06 (C-8); 32,32 (C-2); 32,32 (C-7); 34,37 (C- 22); 36,55 (C-20); 36,91 (C-10); 37,67 (C-I);
40.19 (C-12); 42,70 (C-4); 42,73 (C-13); 46,25 (C-24); 50,55 (C-9); 56,48 (C-17); 57,18 (C-14);
73.20 (C-3); 122,11 (C-6) vd 141,17 (C-5).
(2): Bgt mau trdng, mp. 275-277 °C. ESI-MS:
m/z 577[M+H]+.'H-NMR(CDCl3& MeOD, 500
T^p chiDuac liiu, tdp 19, so 612014 359
MHz, 8H ppm, J Hz): 0,61 (3H, s, H-29); 0,70- 0,79 (9H, m, H-19, 26, 18); 0,84-0,86 (4H, m, H- 9, 24, 14, lb); 0,93 (6H, s, (H-27, 21); 0,96-1,04 (3H, m, H-15b, 22b, 17); 1,07-1,12 (3H, m, H- 23b, 23a, 4b); 1,13-1,31 (5H, m, H-28b, 16b, 28a, 22a, 20); 1,34-1,46 (4H, m, H-17b, l i b , 2b, 1 la);
1,48-1,53 (2H, m, H-7a, 15a); 1,55-1,61 (IH, m, H-25); 1,75-1,85 (3H, m, H-16a, la, 2a); 1,89- 1,95 (2H, m, H-8, 4a); 2,19-2,22 (IH, m, H-12b);
2,30-2,34 (IH, m, H-12a); 3,15-3,18 (IH, m, H- ly, 3,20-3,23 (IH, ra, H-4'); 3,29-3,38 (2H, m, H-5', 3'); 3,47-3,54 (IH, m, H-3); 3,68 (IH, dd, J
= 4.5 va 12.0, H-6'b); 3,78 (IH, <«, J = 3.0 v4 12.0, H-6'a); 4,33 (IH, d,J= 8.0, H-1') va 5,29 (IH, t, J = 2.0 va 2.5, H-6). "C-NMR (CDCl, &
MeOD, 125 MHz, 8c ppm): 11,6 (C-18); 11,7 (C- 29); 18,5 (C-21); 18,7 (C-19); 19,1 (C-27); 19,5 (C-26); 20,9 (C-11); 22,9 (C-28); 24,1 (C-15);
25,9 (C-23); 28,0 (C-16); 29,0 (C-25); 29,4 (C- 2); 31,7 (C-7); 31,74 (C-8); 33,8 (C-22); 36,0 (C- 20); 36,5 (C-10); 37,1 (C-1); 38,5 (C-12); 39,6 (C-4); 42,1 (C-13); 45,7 (C-24); 50,0 (C-9); 55,9 (C-17); 56,6 (C-14); 61,7 (C-6'); 70,0 {C-4'); 73,4 (C-2'); 75,6 (C-3'); 76,2 (C-5'); 79,0 (C-3); 100,9 (C-1'); 122,0 (C-6)va 140,1 (C-5).
(3): Bpt mau trjng, mp. 212-2I3'C. HRMS:
m/z 427.39397 [M+Hj*. 'H-NMR (CDClj, 500 MHz, d„ ppm, J Hz): 4,68 (IH, d, J^2,0, H-29a);
4,57 (IH, s, H-29b); 3,19 (IH, dd, J=5,0 va 11,5, H-3); 2,35-2,40 (IH, m, H-19); 1,90-1,94 (IH, m, H-21a); 1,68 (3H, s, H-30); 1,03 (3H, s, H-26);
0,97 {3H, s, H-23); 0,95 (3H, s, H-27); 0,83 {3H, s, H-25); 0,79 (3H, s, H-28); 0,76 (3H, s, H-24);
0,68 (IH, d, J=9,5, H-5)... "C-NMR (CDCIj, 125 MHz, Sc ppm): 151,0 (C-20); 109,3 (C-29);
79,0 (C-3); 55,3 (C-5); 50,5 (C-9); 48,3 (C-18);
48,0 (C-19); 43,0 (C-17); 42,9 (C-14); 40,9 (C- 8); 40,0 (C-22); 38,9 (C-13); 38,7 (C-4); 38,1 (C- 1); 37,2 (C-10); 35,6 (C-16); 34,3 (C-7); 29,9 (C- 21); 29,7 {C-23); 28,0 (C-15); 27,5 (C-12); 25,2 {C-2); 21,0 (C-ll); 19,3 (C-30); 18,3 (C-6); 18,0 (C-28); 16,1 (C-25); 16,0 (C-26); 15,4 (C-24);
14,6 (C-27).
(4): Tinh the hinh kim mau tr&ig, mp. 156- 158 °C, ao'-146,5 (c, 0,1 MeOH). ESI-MS: m/z 259,05 [M-lNa]*. 'H-NMR (DMSO-rfj, 500 MHz,
SH ppm, J Hz): 4,44-4,46 (IH, m, 1-OH); 4,43 (IH, J, 4-OH); 4,41 (IH, s, 5-OH); 4,26 (IH, d, J^7,5,3-OH); 3,72 (IH, <«, J=6,5 va 10,0, H-5);
3,67 (IH, (, J=3,5, H-3); 3,59-3,61 (IH, m, H.4);
3,47-3,58 (4H, m, H-1 va H-6); 3,39-3,42 (2H, % H-iJ, 1,42-1,47 (2H, ra, H-2'); 1,30-1,36 (2H, m, H-3'); 0,88 (3H, s, H-4'). "C-NMR ( D M S 0 4 125 MHz, Sc ppm): 100,1 (C-2); 69,4 (C-4); 69,2 (C-5); 69,0 (C-3); 63,8 (C-6); 62,1 (C-1); 59,4 (C-r); 31,9 (C-2'); 19,0 (C-3'); 13,9 (C-4').
3. Ban luan ket qua 3.1. H(rp chdi (1)
Hgp chat 1 k^t tinh trong n-hexan 6 d^ng bpt mau trSng, mp. 136-137 "C. TLC cho mpt vet mau tim khi phun dung dich H2SO4 10% trong ethanol va ha nong, co R^= 0,26 (C:M 95:5). Pho khoi luong cho peak ion gia phan tijr m/z 415 [M-t-H]'^ tuang irng vai cong thuc phSn tir C29H50O(414dvC).
Cac pho ID-NMR cho thdy hgp chat 1 mang dac tnmg cOa hgp chat sterol, tra cuu tai li^u [7]
thay trimg khop vofi 6e?a-sitosterol (Hinh 1).
3.2. Hcrp chdt (2)
Hop chat 2 k^t tinh d ^ g b6t trSng trong MeOH, mp. 275-277 °C. TCL cho mpt vet man tim khi phun dung dich H2S04 10% trong ethanol va hg nong, co R^ = 0,39 (C:M 9:1). Ph6 khoi lupng cho peak ion gia phan hi m/z 511 [M+H}'' tirong img vai cong thtic phan ttr CasHeoOe (576 dvC) Cac pho 1 D-NMR cho thdy hgp chat 2 mang dac tnmg cua hgp chat sterol gdn vai mpt don vj ducmg glucose, tra ciru tai lieu [8] thdy triing khop vai daucosteroi (Hinh 1).
3.3. Hap chdt (3)
Hgp chat 3 ket tinh trong n-hexan dudi d?ng bpt mau trdng, mp. 212-213"C. TLC cho mpt vet mau tim khi phun thu6c thir H2SO4 10% trong ethanol va ha nong co R/la 0,33 (H:E 95:5). Phi HRMS cho peak ion gia phan ttr m/z 427.39397 [M+H]'^ phii hgp vgi cong thuc phan tiit CjoHjoO (426.71983 dvC). Ket hgp vcri cac tin hi^u ciia proton va carbon tren ph6 1 D-NMR cho thay hpp chat 3 CO dac tnnjg cua mpt triterpen 5 vong.
That vay, pho 'H-NMR cho thay hgp chat 3 CO hai proton olefin g SH 4,68 (IH, rf, ,^2,0) vi 4,57 (IH, s); bSy nh6m methyl 6 S„ 1,68; 1,03;
360 Tfp chiDuac lieu, tap 19, sS6IMU
0,97; 0,95; 0.83; 0.79 wk 0,76 (3H, s); mgt proton ke nhdm hydroxy d Su 3.19 (IH, dd, 7=11,5 va 5,0) va kho&ng 26 proton khac cua cac nhdm methyl, methylen. Ph6 "C-NMR \k DEPT xuit hi^n cdc tin hi^u cua tdng sd 30 carbon gom 7 methyl, 11 methylen, 7 methin va 5 carbon bgc 4.
Hai carbon anken xuat hi?n d Sc 151,0 va 109.3, trong khi do carbon n6i vdi nhdm hydroxy xudt
hi?n d Sc 79,0.
Vdi cdc dgc dilm tren, du li^u pho 1 D-NMR cua hgp chat 3 mang dac tnmg ciia mgt triterpen dang khung lupan. So sanh d& li?u pho ID-NMR eua 3 vdi tai lifu [9] thiy khdp vdi hgp chit lupeol (Hinh 1). Han nua, phd HSQC va HMBC cung cho thiy cac tuang quan diu thong nhit phu hgp vdi phd ID-NMR.
(3) Lupeol (4) n-butyl-O-^-D-fructopyranosid Hinh 1. Cic hgip ch^t phan l§p tir trdi qudch
proton nhom hydroxy SH 4,26-4,45. Pho "C-NMR 3.4. Hop chat (4)
Hgp chat 4 kit tinh trong CHCI3 dudi dgng tinh the hinh kim mau hdng, mp. 156-158 °C, a"-146,5 (c, 0,1 MeOH). TLC he dung mdi C:M 85:15, phun dung djch H2SO4 10%, trong ethanol va ha ndng tren bep di?n cho 1 vet mdu den cd R/ la 0,52. Pho MS cho peak ion gia phan td m/z 259,05 [M-f-Na]* phii hgp vdi cdng thuc phfin td C10H20O6 (236,13 dvC).
Pho ' H - N M R xuit hi?n cac tin hi$u cua khoang 20 proton, trong dd ed 3 proton nhdm methyl SH 0,88; cdc proton nhdm methylen trong khodng SH 133-1,45; cdc proton nhdm oxy methylen trong khoang SH 3^9-3,58; cac proton nhdm methyl mang ojQ'gen trong khoang SH 3,59-3,72 va cac
va DEPT cho thay hgp chat 4 co tdng so 10 carbon, trong dd cd 1 carbon methyl Sc 13,9; 1 carbon bgc ASc 100,1; 3 carbon methyl mang oxy Sc 69,0- 69,2; 2 carbon methylen Sc 19,0-31,9 va 3 carbon methylen ke oxygen Sc 59,4-63,8.
Dir Ii?u ID-NMR cho thay hgp chat 4 mang dgc trung cCia 1 phan td dudng fructose mang nhdm the hydro carbon bao hda, so sanh vdi tai li?u [10] thay phu hgp vdi du lieu phd cua hgp chat n-butyl-0-^-D-fruetopyranosid (Hinh 1).
Mat khdc, cae phd HSQC va HMBC cua hgp chat 4 cho thdy cac tuang quan deu thong nhat phii hgp vdi cau tnic hgp chat nay, dong thdi chimg td nhdm n-butyl gdn vdi C-2 cua vdng
Tifp chiDuac Uiu, tdp 19, so 612014 361
dirdng fructose qua clu ndi oxy. sitosterol (1), daucosteroi (2), lupeol (3) vd n- 4. Ket luan butyl-O-^-D-fructopyranosid (4). Trong do hgp Td eao elhyl acetat va cao n-butanol, bdn hgp chat 4 lln diu tien phat hign cd trong loai chit dd dirge phan lap va xac djnh cau triic la: 0- Limonia acidissima L.
Tai lifu tham kbio
1. Ph^m Hoing HO (2000), Cay co Viet Nam. Nhi xuk ban Tre, Quyln 2,437. 2. Nguyin Vdn Main, Thach Rachtana, Trii Thanh Tnic (2O09), MQI S5 ttnh cliit d|c tnmg ciia trai qufich vi klii ning chl bi&i nude quach len men, Tgp chi khoa hgc Tru&ngD<?ihpcCdn Tha, l i b , 97-104. 3. Kim K. H., HaS. K., Kim S. Y., Kim S. H. Lee K. R, (2009), Limodissimin A; A new dimeric coumarin from Limonia Acidissima, Bulletin of the Korean Chemical Society, 30(9), 2135. 4, Parthasarathl G., Prabal S., SrabanJ D., Swapnadip (1991), Tyramin derivatives from the fiiiit of Limonia acidissima. Journal of Natural Products, 34(5), 1389-1393. 5. John K. Macleod, Peter D, R. Moeller, B. M. Ramayake B. (1989), Acidissimin, A new limonoid from Limonia Acidissimin, Journal of Natural Products, 52(4), 882-885. 6. Zarga M. H. A. (1986), Three new simple indole alkaloids from Limonia Acidissimin. Journal of Natural Products, 49(5), 901-904. 7. Kovganko N. V, Kashkan Z. N., Borisov E, V., Batura E. V. (1999), '^C NMR spectra of ^-sitosterol derivatives with oxidized rings A and B, Chemistry of Natural Compounds, 35(6), 646-649. 8. Ton N&Liln Hucmg vicgng sir (2011), NghiSn curu thinh phin h<ia hpc ciia than cay co xuoc (Achyranthes aspera L.) a Trd Vinh, Tgp chi khoa hgc Tnrcmg Dgi hoc Cdn Tha, 19b, 56-61.9.
Jamal A. K., Vaacob W. A., Din L. B. (2008), A chemical study on Phyllanthus reticulates, Journal of Physical Science, 19(2), 45-50. 10. Pyo M. K., Sock H., Yun-Choi, Kim Y. K. (2006), Isolation of /j-buthyl-,g-D-fructopyranoside from GastrodiaelataB\ume, Natural Product Sciences, 12(2), 101-103.
Tap chl Duac li^u, t&p 19, s6 6/2014 (Trang 362 - 368)
KHAO SAT TAC DJJNG TANG LlTC VA TAC D^NG CUA CAO XlTONG CA SAU HOA CA TREN MO fflNH TANG ACH) URIC MAU THlTC NGHIEM Tran Thf Minh Tdm', Nguyin Thf Th u Hieffng', Trhn Van Ndm^, Nguyin Minh Diirc'' *
'Dgi hQC YDwc TP. HCM, ^Trung tdm Sdm va Dwc li^u TP. HCM.
^Vi?n YDu<?c hoc Ddn toe TP. Hd Chi Minh Email: [email protected] (Nhan bdi ngay 28 thang 9 ndm 2014)
T6m tit
Nghifin cfiu niy dirge thirc hien nham d ^ h gid tac dyng tang lyc ciia cao xuong c i siu hoa c i tr6n hai mo hinh chu^t boi ki§t Slic Brekhman vi mo hinh chu^t boi trong be boi c6 the diSu chinh tfic dp d6ng, dSng thoi khio sat inh huong ciia cao xuong c^ sau hoa c i len h^m lirgrng acid uric miu tren mo hinh gay tSng acid uric cip v i man 6 chupt nhat triling bang kali oxonat. Ket qui nghien ciiu cho thiy o liau 1,89 g/kg va 3,78 g/kg, cao xirong c i siu hoa cd co tic dyng ting lyc v^ h? acid uric miu trSn thyc nghi$m.
Ttr khda: Cao xuang cd sdu hoa ca. Tdc dmg tdng luc, Tdc dung hg acid uric mdu Summary
Experimental Study on Strengthening and Anti-hyperuricemic Effects of Crocodile Bone Glue in Mice This study was carried out to investigate the slrenthening effect of glue from Crocodylus siamensis Bone on the forced swimming capacity of mice using Brekhman swimming mice model and an adjustable - current water pool, together with its antihyperuricemic effect in acute and chronic hyperuricemic model induced by potassium oxonate. Results of the study showed that crocodile bone glue at doses of 1,89 g/kg and 3,78 g/kg had sUenthening and antihyperuricemic effects.
Keywords: Crocodile bone glue Crocodylus siamensis, Slrenthening effect, Anti-hyperuricemic effect.
362 T0P chiDuac liia, tap 19, so 612014
