Nghien CLFU - Ky thuat
Nghien ciru anh hu'dng cua thong mach sa lac hoan den tinh trang chung va chirc nang tao
mau tren tho thye nghiem
Ngo Quynh Hoa, Nguyen Tran Thj Giang Hirang, Do Thj Phirong
Tnrang dgi hoc Y Hd NoiDat van de
T u nam 1987 cho ddn nay, bdi thudc nghiem phuang Thdng mach sa lac phuang da duge Hpc vien Trung y Thiem Tdy nghien eiru va dp dung didu tr| eho benh nhan tai bidn mach mdu nao (TBMMN) mang lai kdt qua tdt. Nam 2008, bai thudc cung da dugc dp dung tai khoa YHCT - Benh vien da khoa Xanh pdn cho t h i y kdt qua kha kha quan trong phuc hdi chirc nang van dpng cho benh nhan TBMMN vd ehua t h i y ed tae dung phu dang kd nao. Thdng mach sa lac hoan (TMSLH) ed ngudn gde tir bdi Thdng mach sa lac phuang eua Trung qudc da duge gia giam them mdt sd vj vd de tien Igi cho benh nhan trong qud trinh didu tri, thudc duge chuydn sang dang vien hoan. De ddnh gia ea s d khoa hpe vd tinh an todn eua Thdng mach sa lac hoan, chung tdi tidn hdnh nghien cuu nay vdi muc tieu: Nghien ciru inh hwdng cua Thdng mach sa lac hoan len tinh trang chung va chirc nang tao mau tren thd thwc nghiem.
D6\ tu'Ong va phu'O'ng phap nghien
CLPU
Nguyen lieu va ddi tu'O'ng nghien cu'u Nguyen lieu nghien cii'u
*Thudc: Dung djch thudc eho ddng vat thuc nghiem udng duge lam gia b i n g nude c i t theo ty Id lOOg/IOOml, cu the la 10 vien hoan (10g/vien) hda vdi 100ml nudc c i t rdi dem dun va khuly ddu ddn khi edn 50ml la duge, 1ml thude thir tuang duang vdi 5,67g duge lieu.
Thudc thir do khoa Y hpc Cd truyen, Bdnh vien Da khoa Xanh pon eung e l p .
Cdng thuc bai thudc: Tdng trpng lugng bai thudc la 170g, gdm: sinh ky, dia long, nguu t i t , trdn bi, xuyen khung; san tra, cau d i n g , phuc linh, dan sam, u l t kim.
Doi tuKyng nghien cij'u
Thd ehiing Newzealand White, Idng tring, ca hai gidng khoe manh, trpng lugng 1,8 - 2,2kg do Trung tam chan nudi, Vien Kidm nghiem Trung uang cung cap.
Ddng vat d u g c nudi trong phdng thi nghiem 3 5 ngay trudc khi nghien eiru va trong sudt thdi gian nghien ciru b i n g thire an c h u i n danh ridng cho tung loai sue vat (do Vien Ve sinh djch td Trung uang vd cdng ty lien doanh Guyomare'h - VCN san x u l t ) , udng nudc t u do.
Hoa chat nghien cu'u
Dung djch xdt nghiem mau ABX Minidil LMG eua hang ABX - Diagnostics, djnh lugng tren may Vet abe™ Animal Blood Counter.
Phu'O'ng phap nghien cii'u
Thd dugc ehia lam 3 Id, mdi Id 10 con, mdi con nhdt rieng mdt chudng.
Ld chu'ng: udng n u d c c i t .
Ld tn 1: udng thudc thir TMSLH lidu 2,4ml/kg/ngay (13,6g/kg/ngay tuang d u a n g vdi lidu dung cho ngudi, tinh theo he sd 4).
Ld tri 2: udng thudc thir TMSLH lieu 7,2 ml/kg/ngay (40,8g/kg/ngay - g I p 3 l i n lidu d Id trj 1).
Thd dugc udng nudc hoac thudc trong 4 tuan lien, mdi ngay mdt l i n vdo budi sang. Sau 4 tuln, ngung eho thd udng thudc va tiep tuc nudi trong 2 t u l n dd theo ddi, danh gia s u phuc hdi.
Theo ddi tinh trang ehung, cdn nang vd c^c chl sd danh gia chirc nang tao mau (sd lugng hdng e l u , ham lugng huyet s i c td, hematocrit, sd lugng bach c l u , cdng thirc bach cau, sd lugng tieu cau) d cae thdi diem trudc udng thudc, sau 2 tuan, sau 4 t u l n udng thudc vd sau ngung thudc 2 tuln'^'^'^'
XiP ly s6 lieu
Cae sd lieu nghien cu'u d u g c xw ly thdng kd theo phuang phap t - test Student vd test "trudc
32
TAP CHI DUQC HQC - 7/2011 (SO 423 NAM 51)
Nghien CLFU - Ky thuat
- sau" Sd lidu duac bieu didn dudi dgng: X ± SD. Su khdc biet cd y nghTa khi p<0,05.
K§tqua nghien cii'u
Anh hu'dng cua TMSLH d i n tinh trang chung va SIF thay doi can nang cua tho
Sir thay doi can nang cCia tho
Bang 1: Anh hwdng cua TMSLH din thi trong thd
Tinh trang chung
Trong thdi gian thi nghidm, thd d ca 3 Id hoat ddng binh thudng, nhanh nh?n, m i t sdng, Idng mugt, dn udng tdt, phdn khd. Khdng t h i y bieu hien gl ddc biet d ca 3 Id thd trong sudt thdi gian nghien cuu.
Ld chCrng Lo tri 1 Lo tri 2
Th&i gian
Trong Iwang
Jkg)'
(X ±SD)% tang trgng Iwo'ng
Trgng Iwang Jkg) • (X ±SD)
%tang trgng Iwo'ng
Trong Iwang
'jkg)'
(X +SD)% tang trgng Iwgng
P (t -test Student) Truac uong thu6c
Sau 2 tuan u6ng thuoc p(trucyc-sau) Sau 4 tuan uong thu6c
p(tru'6c-sau) Sau ngirng u6ng thu6c
2 tuan p{\rwac - sau)
1,80±0,14 2,00 ±0,19
<0,05 2,26 ±0,17
<0,05 2,33 ± 0 , 3 3
<0,05
21,75
30,70
1,87±0,18 2,23 ±0,23
<0,05 2,46 ± 0,22
<0,05 2,48 ±0,17
<0,05
19,29
31 ,79
1,89±0,17 2,27 ±0,17
<0,05 2,57 ±0,12
<0,05 2,44 ±0,21
<0,05
20,47
36,77
>0,05
>0,05
>0,05
>0,05
Kdt qua d bang 1 cho thiy: sau 2 tuan vd 4 khi nghien ciru. Khdng ed s u khae t u l n udng thudc, trpng lugng thd d ca 3 Id (1 Id dp gia tang trpng lugng thd giu'a chirng va 2 Id trj) deu tang rd ret so vdi trudc cdc Id dung thudc (p>0,05)
Anh hu'cyng cua TMSLH ddn chii'c nang tao mau cua tho
Bang 2: Anh hwdng cua TMSLH din sd lwang hdng ciu trong miu thd
biet ve muc Id chirng vd
Th&i gian
Truoc uong thu6c Sau 2 tuan uong thuoc
p (truc/c - sau) Sau 4 tuan uong thuoc
p (truoc - sau) Sau ngirng uong thuoc 2 tuan
p (truoc - sau) Bang 3: Anh hwdng cua
Th&i gian
Trudc uong thudc Sau 2 tuan uong thuoc
p (trucjc - sau) Sau 4 tuan uong thudc
p (truc>c - sau) Sau ngirng u6ng thuoc 2 tuan
So Iwo'ng hong cau (tri^u/mm ) Lo chCrng
(X ±SD) 5,33 ±0,67 5,17 ±0,36
> 0 , 0 5 5,39 ±0.19
> 0 , 0 5 5,44 ± 0,36
> 0 , 0 5
Lo trj 1 (X ±SD) 5,12 ±0,34 5,12 ±0,29
>0,05 5,19 ±0,33
>0,05 5,28 ±0,20
>0,05 TMSLH din ham lwang huyit sic to I
Ham Lo chCrng (X ±SD) 11,38± 1,21 10,72 ±0,58
> 0 , 0 5 11,68 ±0,60
> 0 , 0 5 11,48 ±0.38
Iwgng huyet sac Lo tri 1 (X ±SD) 10,96 ±0,72 11,15±0,66
>0,05 11,36 ±0,65
>0,05 11,20 ±0,50
Lo trj 2 (X ±SD) 5,18 ±0,45 5,04 ± 0,43
>0,05 5,02 ± 0,56
> 0 , 0 5 5,24 ±0,27
> 0 , 0 5 'rong miu thd t6(g/dl)
Lo trj 2 (X ±SD) 11,26 ± 0 , 9 7 10,82 ±0,84
> 0 , 0 5 11,27 ± 0 , 5 1
> 0 , 0 5 11,08 ±0,69
p (t - test Student)
>0,05
>0,05
>0,05
>0,05
n (t-test Student)
>0,05
>0,05
>0,05
>0,05
p (truoc - sau) >0,05 >0,05 >0,05
TAP CHi DUQC HQC-7/2011 (SO 423 NAM 51) 33
Nghien CLFU - Ky thuat
Bang 4: Anh hwdng cua TMSLH din hematocrit trong miu thd
Hematocrit (%) Thai gian Lo chirng(X ±SD)
Lo tri 1 (X ±SD)
Lo tri 2 (X +SD)
P (t-test Student) Truoc uong thuoc 34,58 ±3,84 34,10 ±2,21 33,94 ±2,95 >0,05 Sau 2 tuan uong thuoc 32,87 ± 1,80 34,86 ±2.81 32,52 ± 2,60 >0,05
p (truoc - sau) >0,05 >0,05 >0,05
Sau 4 tuan uong thuoc 34,14 ± 1,74 33,85 ± 1,72 33,15 ± 1,28 >0,05
p (trudc - sau) >0,05 >0,05 >0,05
Sau ngirng uong thuoc 2 tuan 33,43 ± 1,17 35,20 ± 1,55 33,46 ± 1,72 >0,05
p (truoc - sau) >0,05 >0,05 >0,05
Bang 5: Anh hwdng cua TMSLH din sd lwang bach ciu trong mau thd So Iwgng bach cau (nghin/mm ) Th&i gian Lo chCrng
(X ±SD)
Lo tri 1 (X ±SD)
Lo tri 2 (X ±SD)
P (t-test Student
Truoc uong thudc 10,06 ±2,28 Sau 2 tuan uong thudc 9,43 ±3,15
p (trudc - sau) > 0,05 Sau 4 tuan uong thudc 8,10 ± 2,38
p (trudc - sau) > 0,05 Sau ngirng uong thuoc 2 tuln 8,25 ± 3,26
p (trudc - sau) > 0,05 Bang 6: Anh hwdng cua TMSLH din cdng
10,03 ±1,99 9,05 ± 1,38
>0,05 9,30 ±1,59
>0,05 10,00 ±0,85
>0,05
thirc bach ciu trong miu
12,39 ±4,05 8,90 ± 1,99
>0,05 9,23 ± 1,09
>0,05 9,02 ±2,05
>0,05 thd
>0,05
>0,05
>0,05
>0,05
Cong thirc bach cau
Th&i gian
Lo chirng (X +SD)
Lo tri 1 (X ±SD)
Lo tri 2 (X +SD)
P (t-test Student) Lympho
(%) Trudc uong thuoc 76,78±7,38 Sau 2 tu§n u6ng thudc 75,89±14,33
p (trudc - sau) > 0,05 Sau 4 tuln uong thuoc 78,11±7,85
p (trucjc - sau) > 0,05 Sau ngirng uong thuoc .^-, 22+3 g^
2 tuan ' ' ' p (trudc - sau) > 0,05
Trung tinh (%) 23,22±7,38 24,11±14,33
>0,05 21,89±7,85
>0,05 22,78±8,91
>0,05
Lympho (%) 76,70±9,72 81,90±7,34
>0,05 81,10±12,89
>0,05 80,40±12,25
>0,05
Trung tinh (%) 23,30±9,72 18,10±7,34
>0,05 18,90±12,89
>0,05 19,60±12,25
>0,05 Bang 7: Anh hwdng cua TMSLH din sd lwang tiiu ciu trong mau
Lympho (%) 70,8013,40 77,20±7,18
>0,05 76,00+10,33
>0,05 79,40±6,58
>0,05 thd
Trung tinh (%) 29,20±13,40
22,80±7,18
>0,05 24,00±10,33
>0,05 20,60±6,58
>0,05
>0,05
>0,05
>0,05
>0,05
So Iwg'ng tieu cau (nghin/mm ) Thai gian Lo chCrng
(X ±SD)
Lo tri 1 (X +SD)
Lo tri 2 (X +SD)
P (t-test Student) Trudc u6ng thudc
Sau 2 tuan uong thuoc p (trudc - sau) Sau 4 tuan uong thuoc
p (trudc - sau) Sau ngirng uong thuoc 2 tuan
p (trudc - sau)
296,33 ± 63,55 280,89+.99,30
>0,05 279,56 ± 36,77
>0,05 328,67 ± 110,62
>0,05
316,90± 51,81 329,90 ±84,06
>0,05 332,20 ± 87,08
>0,05 289,75 ±40,89
>0,05
280,60 ± 8 2 , 7 3 291,80 ± 5 1 , 0 3
>0,05 298,60 ± 68,91
>0,05 279,00 ±66,19
>0,05
>0,05
>0,05
>0,05
>0,05
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TAP CHi DUQC HQC - 7/2011 (SO 423 NAM 51)
Nghien CLFU - Ky thuat
Ket qua cho thiy: sau 2 tuln, 4 tuln vd sau 2 tuan ngirng udng TMSLH, tit ca cdc xdt nghidm danh gid chuc ndng tao mdu (sd lugng hdng c l u , hdm lugng huydt s i c td, hematocrit, sd lugng bach c l u , edng thirc bach c l u vd sd lugng tieu elu) d ea Id trj 1 (udng TMSLH lidu 2,4ml/kg/ngay) vd Id trj 2 (udng TMSLH lieu 7,2ml/kg/ngay) ddu khdng cd s u khde biet cd y nghTa so vdi Id ehung vd so sdnh giu'a edc thdi diem trudc va sau khi udng thude (p>0,05).
B a n l u a n
Anh hu'ong cua TMSLH d i n tinh trang chung va SIF thay ddi can nang cua thd
Can nang cua t i t ea cae thd ddu tang so vdi trudc khi nghien eiru. Mue dp gia tang trpng lugng cua thd d ea 2 Id trj trong 4 t u l n vd theo ddi 2 t u l n sau khi ngung thude khdng cd s u khac biet so vdi Id ehung. Nhu vay, ed the kdt ludn r i n g TMSLH khdng lam anh hudng x i u ddn tinh trang ehung va muc dp tang trudng cua thd khi udng thudc lien tue trong 4 t u l n , ke ea d Id thd udng lieu eao g I p 3 lan lidu ed tae dung tuang duang tren ngudi,
Anh hu'dng cua TMSLH ddn chii'c nang tao mau
Mau la mdt thdnh p h i n r i t quan trpng eua ca the Cdc chi sd trong xet nghiem td bdo mau ngoai vi ed gid tr| ldn trong vide danh gia chuc nang tao mdu '^•^•'''
Sau 2 t u l n va 4 t u l n udng thude lien tue, cung nhu sau khi ngung thudc 2 t u l n , cac chi sd trong xet nghiem te bao mau ngoai vi (sd luang hdng c l u , ham lugng huydt s i c td, hematocrit, sd lugng bach c l u , cdng thire bach c l u vd sd lugng tieu elu) trong mdu thd d Id tri
1 va 2 ddu khdng cd s u khae biet ed y nghTa thdng ke so vdi Id chung vd so vdi trudc khi dung thudc (p>0,05).
Vay TMSLH lieu 13,6g/kg/ngay vd lieu 40,8g/kg/ngay khdng gay anh hudng ddn chuc nang tao mdu cua thd.
Ket luan
TMSLH lieu 2,4ml/kg (13,6g/kg/ngdy - tuang duang lieu dung tren ngudi) vd lieu 40,8g/kg/ngay g I p 3 l i n (7,2ml/kg) khdng gdy anh hudng ddn tinh trang ehung, s u tang trpng lugng cua the va chuc nang tap mdu khi cho thd udng lien tuc trong 4 t u l n .
Summary
"Thong mach so lac hoan" extract for treatment of cerebral infarction after the acute period was evaluated for the effects on haematopoietic function. The extract was administered by oral route to rabbits at doses of 2.4ml and 7.2ml/kg b.w./day for 4 weeks. All the animals survived with no significant changes in general signs, growth and hematological examinations (red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, white blood cell count, leukocyte formula and platelet count). "Thong mach so lac hoan" extract exhibited no effects on the constitution, growth and haematopoietic function In rabbits by oral administration.
Keywords: "Thong mach so lac hoan"
experimental rabbits administration.
Tai lieu tham khao
1. Bp mon Duoc ly, Trudng Dai hoc Y Ha NQI, Duoc ly hoc lam sang, Nha xuat ban Y hgc, (2003).
2. Nguyen The Khanh, Pham TCr Duong, Xet nghiem sir dgng trong lam sang, Nha xuit ban Y hgc, (2001)
3.Bach Qudc Tuyen, Huyet hoc, (Tap 1), Nha xuat ban Yhgc. (1978).
4. Bach Quoc Tuyen, Ky thuat xet nghiem huyet hpc va truydn mau, Nha xuit ban Y hgc. Ha Ngi, (1984), tr 9-89.
5. World Health Organization, Working group on the safety and efficacy of herbal medicine. Report of regional office for the western pacific of the World Health Organization, (2000).
P h a n l a p v a . . . (Vip theo trang 31)
Phan lap va xac dinh cau tnjc quercetrin va afzelin tir cay Tam giri- Taxillus chinensis (DC.) Dans song tren cay ggo Tap chi Dwgc hgc so 3 (2011)- tr.37
4. Agrawal P K : 13C-NMR of flavonoids (1989) Elsen/ier Science Pulishers. p. 144
5. Tae-Jaek Han, Myum- Ho Bang, Ock-Kyoung Chun, Dae-Ok Kim, Chang-Yong Lee and Nam-ln Baek: Flavonol Glycosides from the Aerial Parts of Acehphyllum rossi and Their Antioxidantes. Arch.
Phanv. Res Vol.27 (4) 390-395 (2004)
