TRUCfNG DAI HOC DONG THAP Tap chi Khoa hpc so 27 (08-2017) T 6 I UtI HOA QUY TRINH CHIET XUAT VA KHAO SAT HO^T TINH KHANG KHUAN, KHANG OXY HOA CUA CAY DIEP CA (Houttuynia cordata)
• Pham Ngpc Khoi", Phung Thanh Sonn Tom tat
Nghien cihi ndy da tdp trung vdo viec khdo sdt hieu sudt thu hdi dich chiet edy diep cd (Houttuynia cordata) vdi cdc diiu kien khdc nhau, dong thdi khdo sdt hogt tinh khdng khudn, khdng gxy hda ciia dich chiit edy diip cd. Kit qud eho thdy ndng dd ethanol chiit 96°; thdi gian chiit 2 gia, nhiet do chiit 50°C eho hiiu sudt chiit tdch dich chiit edy diip cd Id cao nhdt. Dich chiit edy diip cd cd khd rmg khdng dugc cdc chiing vi khudn Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa^
mirc trung binh trong diiu Men thi nghiim. Ngodi ra, dich chiit cdn cd khd ndng khdng oxy hda cSngd mirc trung binh (IC^g = 62.237 mg/ml) so vdi mdu ddi chimg Id vitamin C (IC^g - 23.374 mg/ml) dcdc ndng do thu nghiem khdc nhau.
Tie khda: edy diep cd, khdng khuan. khdng oxy hda, IC^g
1. D | t van de khang lai su tang sinh ciia eac dong te bao ung thu Cay diep ca (Houttuynia cordata) eon eo ten trong eac thu nghi?m in vitro va in vivo gay unglhu khac la cay giap e a , l a g i ^ h a y ngu tinh thao [7]. tren chugt [5]. Vi the, miie tieu ciia nghien cim n%
Trong nhiing nghien cuu gan day eua eae tac gia la nhSm toi uu hoa eac yeu to anh hucmg len hi§u trong nuoe da ehung minh thanh phan hoa hpc suat thu dich chiet cay diep ca, dong then khao sat ciia diep ca eo rat nhieu hpp chat sinh hpe thupc khanang khang mpt sovi khuan gay b^nhvakhang nhom flavonoid, tinh dau, alkaloid va mpt so hpp
ehat khae [1], [2], [7]. Diep ca la mpt lo?ii rau rat quen thupc trong bua an hang ngay eua nguoi Viet Nam, ngoai cong dung dupe xem nhu mpt vi rau ngon thi diep ea eon eo rat nhieu nhiing cong dyng khac. Theo Dong y, diep ca eo vi dang, tinh on, tae dung vao eae kinh maeh dai trang va phe, vi vay eo tae dyng giai nhi^ va giai dpe, lam giam sung viem, tri ung nhpt, dom day d|c vang xanh, tri ho ra mau, giai dpe ung nhpt ngoai da, chiia vet thuong do rSn ein hay eon trung ein, lam thong thoat khi ii dpng, giup tieu hoa tot va lpi tieu, dae biet tac dyng chiia benh tn rat hieu qua, do c6 kha nang b ^ ve lam ben tiianh maeh [1], [6], [7], [9], Cae nghien euu gan day cua cac tae gia nude ngoai da chi la rang diep ca eo tae dung ehong lai virus Herpes type I va II, ngan c ^ su sinh san eua cac vi khuan Streptococcus pneumonia (gay sung phoi) va Staphylococcus aureus (gay mun nhot c6 mu), nhung tae dung tren eac vi khuan Shigella, Salmonella va Escherichia coli (gay kiet ly, tieu
oxy hoa eiia dieh chiet cay diep ea trong dieu ki?n nuoi trong va nghien euu tai mien Nam Vi?t Nam.
2. V^t lieu va phinmg phap nghien ctru 2.1. Doi turoug nghien cihi
Cay diep ea (nguyen lieu duoc thu mua o sieu till Lotte Mart, Qu|n 7, tiianh pho Ho Chi Minh) de dupe d ^ bio chat lupng dau vao nen nguyen lieu ehi duoc thu mua duy nhat mpt lan va dupe su dung cho toan bp qua trinh thyrc hiin nhu la nguon nguyen lieu ehinh de tach dieh chiet va su dung dich ehiet do de k h ^ sat hoat tinh khang m$t so vi khuan (cae ehung vi khuan dupe cung cap tir Bp mon Cong nghe sinh hoc, Khoa Khoa hoc tog dung, Trudng Dgi hpe T6n Diic Thing, thanh pho Ho Chi Minh) va dong thdi k h ^ sat ca tinh khang oxy hoa ciia dich chiet.
2.2. Xac dinh ty 1^ trong l u ^ g va dO am la diep ca [3]
-Tyli trgng lugng
Xae finh ty lp trpng lupng phan su dung so vdi tong trpng lupmg cay diep ca. Khdo sat dupe chay) tiii lgi yeu hon nhieu [8]. Ben canh do, dich tien hanh ngau nhien vdi 3 lan IJp, moi lan 100 g ehiet diep ea eon cd tae dyng khang lai qua trinh cay diep ca. Dung tay tach phiin la su dyng ra khoi oxy hda, dot bien gen va tham chi cdn ed kha nang cay diep ca. Phuong p h ^ tien hanh: can tong trgng lupng (m,); taeh rieng phan sii d\ing, ghi nhan trpng 'Tmdng Dai hoc Y khoa Pham Ngpc Thach lupng (m^); ehi ti8utheo doi: ghi nhan cac so li^u '"' Tnremg D31 hpc Ton Ehic Th^g y^ trpj^g jj^^^g p ^ ^ gU dyng.
T R U Q N G DAI H O C DONG THAP Tap chi Khoa hoc so 27 (08-2017;
Phan su dyng (%) = m^/m, x 100.
- Do dm Id diep ed
Khao sat dupe hen hanh vdi 3 lan lap lai. Dung nhipt dp eao (105°C) de tach am ra khoi nguyen heu mau. Phuong phap tien hanh tiii nghiem: can trpng lupng ban dau (m,); dem ph4n sir dyng say d 105°C cho den khi trong lupng khong doi, can ghi nhan (HL); ehi tieu theo doi: trpng lupng trudc va sau khi say:
Dp am phan su dyng (%) = (m^ - ni;)/m, x 100.
2.3. Khao sat kha nang thu hoi dich chiit tii la diep ca vdi cac dieu kien khac nhau [3], [10]
Trong nhiing nghien cuu trude day da cho thay dieh chiet la diep ea ed chua nong do Idn hogt ehat sinh hpe thupc nhdm flavonoid [2] Vi the, trong nghien eiru nay da dinh hudng muc heu ehinh la xae finh cac fieu kipn toi uu nhat eho viee tach chiet thu hoi dieh chiet chua flavonoid tir la fiep ea de xac finh dupe tong ham lupng flavonoid trong eac fieu kiSn tach ehiet khae nhau nhir sau:
- Theo nong dp ethanol trong dung mdi ethanol - nude: 70°, 80", 96".
- Theo thdi gian: 1 gid, 2 gid, 3 gid, 4 gid.
- Theo nhi^t dp: 40"C, 50°C, 60°C, 70"C.
- Theo so lan chiet' 1 lan, 2 lan, 3 lan Quy trinh thuc hien ehiet dich la diep ca bang dung moi ethanol - nude nhu sau: la diep ca —»say kho —> xay nhuyen thanh bpt —» ngam kiet trong dung mdi etiianol - nude —> lpc bd can —> dich chiet.
2.4. Phirorng phap xac dinh ham lu'tfng flavonoid [3], [ID]
Tong flavonoid dupe xac dinh theo phuong phap so mau nhu mieu ta eua Chang va cong su (2002) dyca tren nguyen tie la hpp chat flavonoid se t90 phue mau vang vdi dung fich AlCl^. Phan ung nay dung de xae dinh ham lupng flavonoid tong Cudng dp mau ty lp tiiuan vdi ham luong flavonoid dupe xae dinh d budc sdng 415 nm. L ^ phucmg hinh dudng ehuan: lay 10 |ig quercetin hda tan ti'ong ethanol 80" sau do pha loang ra eac nong dp 20, 40, 60, 80, 100 Mg/ml, Hut 0,5 ml dung dich sau khi pha loang bo sung 1,5 ml ethanol 95°, 0,1 ml AlCl,, 0,1 ml CH3COOK va 2,8 ml nude cat.
Sau dd hon hpp dupe lie deu va de on dinh d nhi?t 10 phong trong vdng 30 phiit, roi ti^n hanh do tren nay UV-Vis d bude sdng 415 nm, mau tring sir iyng lanude eat Lay 0,5 ml fich chietflavonoid va
lam tuong ty nhu eac budc lap phuong trinh dudng chuan Ham lupng flavonoid tong (pg) ehiet tren 1 g nguyen lieu tinh theo edng thiie sau:
F (ng/g) - (a x V X k X 100) / [m X (1 - w)]
Trong do: a la ham luong quercetin (ng/1) duoc xae finh tir phucmg trinh dudng chuan, V la the tich fich chiet tu m (g) la fiep ea (ml), k la he so pha loang, m la khoi lupng mau thi nghiem (g), w la dp am eiia mlu
2.5. Khao sat tinh khang khuan cua dich chiet la diep ca [3,4,10]
Myc fich khao sat la danh gia so bd hoat tinh khang khuan ciia dich ehiet la fi6p ea. Phuang phap tien hanh khao sat nhu sau:
- Mdi trudng nudi vi khuan: moi trudng cao thit - pepton sau khi pha va h ^ khii triing, cho vao dia petri cd day phing va dat tren be mat phang de ed bl day dong nhat khoang 4 mm. De nguoi d nhiet dp phdng.
- Chuan bi dieh khuan: khu§n lae vi khuan tren moi trudng phan lap duoc lay hda tan vao 10 ml nude muoi sinh ly da h ^ khu triing, lac deu va so do due vdi ong mau ed dp due tuong duong mat do 108 CFU/ml (CFU, colony-forming unit, so luong don VI hinh thanh khuan lac).
- Trai vi khuan len mat thach dung micropipet hut 200 pi fich vi khuan bom vao dia mdi trudng da ehuan bi sin. Dung que cay trang, trang deu vi khuan tren mat thach, tiep tuc cho den khi mat thach khd.
- Chuan bi fich thii. hoa tan dieh ehiet vao ethanol d eac nong do 1%, 5%, 10% Chuan bi dia giay: dung dung cy elt fi^a giay dang trdn, dudng kinh 6 mm. Dem hap khii tning d 121°C trong 20 phut. Dem say khd eho den khi su dyng.
- Thu nghiem kha nang khang khuan dung kep gip dia giay da hap khu tning dat len be mat dia petri da trai khuan. Khdng dat eae fi'a giay qua sat nhau va each thanh dia petri 1 em. Nhd triie tiep dung fich mau len dia giay. The tich fich ehiet su dung tren moi fia la 100 \i\. Dem eac fia petri u d nhipt dp phdng, sau 18 - 24 gid tien hanh thu nhan ket qua.
- Cac ehi tieu theo ddi: dudng kinh vdng khang khuan sau 18 - 24 gid u.
2.6. Khao sat ti'nh khang osy hoa ciia cao chiet tu- dich chiet la diep ca [3], [10]
Myc tieu thi nghipm nhim danh gia muc dp
77
TKUCJNG DAI HOC DONG THAP Tgp chi Khoa hpc s6 27 (08-2017) khang oxy hda cua fich ehiet tir la fiep ea so vdi
vitamin C d ciing nong dp tiii nghipm. Phuong p h ^ DPPH dupe sii dyng trong nghien cim nay nhim de xac finh boat tinh khang oxy hoa duoc mo ta trude dd bdi Shimada va cpng sy (1992). Tliuoe thii DPPH dupe pha che ngay trudc khi tien hanh tiii ng^pm de han che anh hudng den ket qua thi nghipm. Phuang phap tien hanh Ihi nghipm nhu sau:
- Chuan bi mlu: can lupng dich chiet cho vao methanol theo Bang 1, lae eho dieh ehiet tan hoan toan trong dung mdi.
B ^ g 1. H ^ linjng va nong do dich chiet Ii diep ci N6ng do dich
cM^t (mg/nd) 20 40 60 80
LiK^ng dich chiet The tich metliasol (mg) (nd)
200 10 400 10 600 10 800 10 - Chuan bi thuoc tini: can 2,7 mg DPPH eho vao binh dinh muc 25 ml. Them 20 ml methanol, lac m^nh eho tan het, finh mue bang methanol den 25 ml. De on finh trong toi 30 phiit, 4°C.
- Chuan bi dung dich vitamin C: can mpt luong vitamin C tiieo Bang 2 vao binh finh miic 10 ml. Them 7 ml methanol vao lie manh cho tan bet, dinh miie den 10 ml.
Bang 2. H ^ lir^ng va odog 0^ vttamiD C Nong do vitamin
C (mg/ml) 10 30 50 70
Luong vitamin C The Iich methanol (mg) (ml)
100 300 500 700
10 10 10 10 - Chuan bi thi nghiem bing each eho vao ong nghipm 1 ml dung fich vitamin C, 1 ml dung fich DPPH trong metiianol va 3 ml methanol roi de trong bdng toi 30 phiit. Tien hanh do dp hap thu
^^^IM ° ^^^^ sdng 515 nm. Tien hanh tiii n g h i ^ tuong ty cho dich chiet la fiep ca. Tuong tu vdi ong doi chling chi ehua 1 ml dung fich DPPH trong methanol va 3 ml methanol, do bude song hap thu
*^^ch^- C ^ ^^^ tiieo doi: ghi nhgn dp hap tiiu ciia cac mau tiii nghiem. Ket qua duoc bieu tiii bing % kim ham DPPH tiieo edng tiiiie sau:
K i m h a m D P P H ^ d - O D ^ , / O D ^ ^ ) x 100
- Trong dd: OD^^^ la dp hap tiiy quang ciia mau kiem soat, OD^j^^ la dp hap tiiy quang eiia mlu can xae dinh.
2.7. Phmmg phap xii- ly so lipu Tat ca cae thi nghidm dupe bo tri 1 ^ lpi 3 ian de dam bao tien hanh phan tich ANOVA. So lieu dupe phan tich ANOVA bing phan mem xii ly so lieu thong ke ehi^en dyng SAS 8.0. Kiem dinh Tukey dupe thue hipn d^ danh gia miic dp khacbiel cd y nghia giiia eac gia tn vdi miic y nghiaP< 0,05,
3. Ket qua
3.1. Xac dinh ty le trong luong va d^ am la diep ca
-Tyli trgng Iieang
Bing 3. Ty le li diep ci so yin tong tryng liryng L k J 2 3 l^ng
lgp lai btah
% li 17,29 ± 21,56 ± 22,71 ± 20,52±
diepca 0,32 0,15 0,53 0,26 La diep ea ed ty Ip trpng lupng trung binh (20,52%) trong ehi Hguttuynia, nhung l^i kha tii^ so vdi tong trpng lupng toan e ^ . Vi v?^ de d^t dupe fiep ea mong muon tM can khoi lupng tuong doi Idn fiep ca.
- Do dm la diip cd
Bing 4. B6 im cua li di^p ci Lan lip
lai Thing
binli
%dQ amli diepci
74,01 ± 76,21 ± 69,38 ± 73,20±
0,23 0,02 0,42 0,09 Dp am cua la fiep ea tuong doi cao (73,20%), dya vao do am do duoc cd the xac finh dupe thai gian can thiet cho qua trinh say de dat hi^u qui cao nhat ed tiie.
3.2. Khao sat hi^u su^t thu bam lugiig flavonoid tong dua trSn cac dieu Iden khac nhau
- Khm sdt theo ndng do dung mdi
111
Do tbi 1. Gia tri ham lin;nig flavonoid tong khi khio sit tbeo ndng dp dung moi
TRU6NG DAI HQC D 6 N G THAP Tgp ch( Khoa hgc so 27 (08-2017) V§y vdi nong dp etiianol la 96" eho kha nang
thu hoi flavonoid la eao nhat. Tuy nhien, d hai nong dp edn lgi van cd the sii dyng lam nguon dung moi tiiay the dupe.
- Khdo sdt theo thdi gian
mi
Do thi 2. G^ tn hikm lirpng flavonoid tSng khi kbao sit theo thM gian Tu 1 gid tdi 2 gjd ta t h ^ ham lupng flavonoid tang len, nhung sau 2 gid thi lupng tang khdng nhieu, va tu 3 den 4 gid thi hau nhu khong tang niia. V ^ tiidi gian toi uu cho moi 1 ^ ehiet la 2 gid.
- Khdo sdt theo nhiet dd
I
I s -I
Bo tbi 3. Gii tri bim lirpng flavonoid tong kbi khao sif theo abi|t d$
Vay nhiet dp toi uu thu dupe ham lupng flavonoid tong eao nhat la 50''C, neu vupt qua ngudng 50''C thi ham lupng flavonoid khong tang nua ma bat dau g i ^ xuong.
- Khdo sdt theo sd lan chiet trin cimg mgt lugng diip cd
I ' ill
liii 1 lin 2 LAII 3 Tliut^iiiiti) dfing lgi B(t thj 4. Gii tri him lirgrng flavonoid tong khi khao
sit theo sd lln chiet trSn cikng m$t lirgrng di^p ci
d 2 lan dau ham lupmg flavonoid thu dupe la tuong duong nhau. Nhung d lan thu 3 thi lupng thu dupe giim fi gan mpt niia. V ^ so lan toi uu tai su dyng 1^ eiing mpt lupng fiep ea eho qua trinh chiet la 2 lan.
Di^a tren cac fieu kipn ve nong dp, thdi gian, nhiet dp, s6 1 ^ su dyng lgi ciing mpt lupng fiep ca ta cd dupe fieu kipn toi uu de d&t dupe ket qui tot nhat v^ mgt tach ehiet thu ham lupmg flavonoid tong la chiet bing dung mdi ethanol vdi nong dp 96°
trong 2 gid d nhipt dd SO^C va vdi ciing mpt lupng fiep ca can dupe thi nen If^ lai viec thu h6i 2 lan.
3.3. Khao sat kha nang khang khuan cua dich chiet tu- la diep ca
Bing 5. Ket qua thf n g h i ^ do vong khing khuan Du-jmg ldnh i. i - i ' i. T X Dmmgkmli vong o mau . , x ^t.
khingkhuSn vong"man do, (cm) chimg (cm) Vikbuan
sir diing Staphylococcus
1,28 ± 0,01 0,10 ±0,01 Escherichia coU 1,42 ± 0,01
Pseudomonas
aeruginosa 0,10 ±0,01
Miie dp khang khuan eua chat cd the chia lam ba e ^ b?ie, dya vao dudng kinh vdng khang khuan Bing 6. Cic cip bSc cua mirc do khang khuan dua
vio dirdng kinh vdng khing khuan C6 tbi Khang Khang kbing khuin khu4n khuan trung binh m^nb Budng kinh
v6ng l(Mng khuan
< 1,0 cm 1,1-1,5 cm > 1,6 cm Dich ehiet tii la fiep ca cd tae dyng khang cac ehung khuan thir nghipm Staphylococcus aureus, Escherichia coli va Pseudomonas aeruginosa.
Vdng khang khuan Idn d mue dp khang khuan tnmg binh, tuy nhien xae suat nhan dupe ket qua khdng cao do nhihig yeu to khach quan nhu fieu kipn eay, bdo q u ^ mau khdng dung vdi tiiu ehuan dan den hinh anh ehyp dupe khdng nhieu. Ben cgnh do, mau doi chung khdng sur dyng hda chat, ehi cay giay khang khuan vao ti-ong mdi trudng nen khi ehyp anh khd thay vdng khang khuan. Tu ket qud thu dupe, cd the sir dyng fiep ca nhu mpt chat ed kha nang khang mdt so loai vi sinh v^t gay bpnh.
TRUCJNG DAI HOC DONG THAP Tap chi Khoa hoc sd 27 (08-2017) 3.4. Khao sat tmh khang oxy hoa cua dich
cay diep ca
- Kit qud xdc dinh dudng chudn tren vitamin C vd kit qud khdo sdt tren dich chiit Id diep cd
Cae nong do vitamin C va phan tram lic ehe DPPH dupe bieu tiii dudi dang dudng thang vdi phuong trinh y = 0,8919x+25,585, vdi hp so tuong quan R^ == 0,98728. Tir do thi ngo^ suy gia tri IC^^
Bang 7. Ket qua do OD vi % uc ch^ DPPH
cua vitamin C la 27,374 mg/ml. Vitamin C dugc tiling lam ehat ehuan de so sanh vdi mlu e ^ ^j ehieu la eao chiet tir la fiep ca, Trong khi do, cac nong dp dich chiet tula fiep cava phan tram uc die dupe bieu thi dudi dang dudng thang vdi phutmg trinh y - l,0377x -14,583, vdi he so tuong quan R^ = 0,9517 Tir do thi ngoai suy gia tri ICj^ cua dich chiet tir la fiep ca la 62,237 mg/ml.
cua mau vitamin C va dicb chiet li diip ci Vilamm C
N^ng dQ (mg/ml)
% iic Ch4 DPPH
10 35,913 ±0,02
30 51,984 ± 0,01
50 66,667 ± 0,02
70 90,476 ±0,0!
Dich chiit Id diep cd N6ng do (mg/ml)
% lie che DPPH
20 6,349 ±0,03
40 22,421 ±0,01
60 56,151 ±0,02
80 64,286 ±0,02 - So sdnh kit qua khdo sdt khd ndng khdng
oxy hod cua vitamin C vdi dich chiit tie la diip cd:
Nong dp de lie che 50% DPPH trong mau cua vitamin C la 27,374 mg/ml trong khi nong dp de lie ehe 50% DPPH trong mau eua dieh ehiet tit la fiep ea la 62,237 mg/ml, V|y ket luan kha nang khang ojq^ hoa cua la fiep ca thap hem eua vitamin C nhung van dupe xem la d mite trung binh Vdi ket qua thu dupe fich figp ca khong the su dyng de
lan Dich ehiet diep ea ed kha nang khang cac chling Staphylococcus aureus, Escherichia coh va Pseudomonas aeruginosa, tuy nhien chi d muc trung binh. Ngoai ra, nghien ciiu nay edn kh^ sat hoat tinh khang oxy hda cua fich ehiet tir la diep ca dua vao phuong p h ^ DPPH (ICj^ = 62,237mg/
ml) cd kha nang khang oxy hda d muc thap hon so vdi vitamin C (IC^^ = 27,374 mg/ml). NghiSn cmi nay kien nghi can tiep tue khao sat de xac finh dieu thay till mpt so chat khang o^q* hda manh, nhung kipn toi uu hon cho viee tach ehiet thu ham lining cd the su dyng nhu mpt chat hd trp eho viee khang
oxy hoa vdi eae edng dyng ho trp fi kem.
4. Ket lu$n
Dya vao cae dieu kien khao sat, ta ed the chpn dupe dieu kien toi uu cho qua trinh tach ehiet diep ca de dat dupe hipu qua eao nhat \
flavonoid tong, ciing nhu tim kiem lo^ dung m5i mdi nham nang eao hipu qua kinh te trong ck qua trinh chiet fich tir la fiep ca. Ti^ nhien ehung tacan phan tich them ve thanh phan hoa hpc trong di^
ca va khao sat th§m kha nang khang khuan doi voi cae ehiing vi sinh vpt mdi nham md rpng them khi hieu suat thu ham luong flavonoid tong cao nhat i^sng khang khuan eua cay fiep ca. Nghien cuu la chiet bang dung mdi ethanol vdi nong dp 96° dyng fiep ca nhu la mpt thanh phan trong eac lo?i trong 2 gid d nhiet dp 50''C va vdi cung mot luong thyc pham chiie nang, eae lo^ tiiuoc phyc vu cho diep ea can dupe thi nen lap lai viee thu hoi 2 "^u cau cua xa hdi./.
Tai lieu tham khao [1]. Pham Hoang Hp (2006), "Cay cd vi thudc d Vi$tNam", NXB Tre.
[2]. Hoang Thanh Huong, Tran Quynh Hoa, Ha Viet B*o, Nguyen Danh Thue (2002), "Gop phan nghien eiiu tiianh phan flavonoid ehiet xuat tii la eay fiep ea Houttuynia cordata Thunb cua Vi?t Nam", Tgp chi Dugc hgc, tap 317, (so 9), tr 13-15
[3]. Pham Ngpc Khdi, Le Trpng Nghia (2016), "Khao sat cac fieu kipn tiiu hoi fich ehiet vaho?t tinh khang khuan, khang oxy hda ciia djch ehiet bap e ^ tim {Brassica aleraceaY', Tap chi Khoa hoc Yersin, (so 01), tr. 23-29
^ [4]. Pham Ngoc Khdi, Nguyen Biii Minh Tam (2016), "Khao sat kha nang khang khuan eiiadich chiet bromelain tii eay dira (Ananas comosus) tren vi khuan Shigella va Salmonella ung dyng ti'ong
RUflNG DAI HOC DONG THAP Tap chf Khoa hpc SO 27 (08-2017) hong ngCia va dieu tri b$iih toong tieu hoa". Tap chi Y hoc Thanh phd Hd CM Minh, (tap 20, Phu an so 5), tr 21-26.
[5]. Lai K. C , Chiu Y. J., Tang Y J , Lin K, L,, Chiang J. H , Jiang Y. L , Jen H E, Kuo Y H., Lgamaya S., Chung J. G , Yang J S. (2010), "Houttuynia cordata Thunb extract inhibits cell growth nd induces apoptosis in human primaiy colorectal cancer cells". Anticancer Res., (30), p. 3549-3556.
[6] Li J., Zhao F. (2015), "AnO-inflammatoiy functions of Houttuynia cordata Thunb. and its ompoundsj A perspective on its potential role in rheumatoid arthritis", Exp Ther Med., (10), p. 3-6.
[7]. D6 Tat L(n (2001), "NhOng cay thuoc va vi thudc Viel Nam", ]vKB Y hoc.
[8]. Lu H., Wu X., Liang Y, Zhang J. (2006), "Variation in chemical composition and antibacterial ctivities of essential oils ftom two species olHouttuyniB, Thunb ", Chem. Pharm. Bull, (54), p. 936-940.
[9] Satdiakam S., Chung W O., Promsong A., Nittayananta W. (2015), "Houttuynia cordata lodulates oral innate immune mediators: potential role of herbal plant on oral health", OralDis., (21), .512-518.
[10]. Nguyen Minh Cam Tien, Ph?mNgocKh6i (2016), "Nghien cira hoat tinh khang khuan, khang am, khang oxy hoa cua hgp chat polyphenol chiet xuat tu re cay muop gai (Lasia spinosa L.)", Tap chi 'hoc Thdnhphd Ho ChiMinh, (tap 20, phu ban so 20), tr. 436-446
OPTIMIZING THE EXTRACTION AND EWESTIGATEVG THE ANTIBACTERIAL, ANTIOXIDANT EFFECTS Of HOUTTUYNIA CORDATA
Summary
The study investigates efficiency of the extract obtained from fish mint [Houttuynia cordata) nder different conditions, and its extract's antibacterial, antioxidant The results show that with 96°
thanol, under 2 hours of extraction, at temperature 50°C the extrachon is most efficient The obtained xtract can resist Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa at average level in xperimental conditions. Also, it can resit oxidants at average level (ICJQ= 62.23 7mg/ml) m comparahon rtth the control sample of vitamin C {iC^ 13i.'ilA mg/ml) under different expenmental concentrations.
Keywords; Houttuynia cordata, antibacterial, antioxidant, \C^.
Ngay nhgn hai. 20/3/2017; Ngay nhan lai. 3/5/2017; Ngay duyet dang07/6/2017.
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