TAP CHi CONG THirUNB
TOI ITU HOA DIEU KIEN TRICH LY ANTHOCYANIN Tl/ HOA DAU BIEC
(CLITORIA TERNATEANL.)
• MAI HUYNH CANG - N G U Y I N XUAN PHUdNG - DOAN THj TUYET TRINH
TOM TAT:
Nghien cvfu toi ifu hoa dieu kien trich ly anthocyanin tu" hoa d|u bi6'c (Clitoria Ternantea L.), kha nang khang oxy hoa cua anthocyanin trong dich chiet. Nguyen lieu hoa dau biec difdc kiem tra dac tinh hoa ly, ham IiTdng tro, dp am cua nguyen lieu tnfde khi khao sat qua tnnh trich Iy anthocyanin. Cac di^u kien toi du. de trich ly anthocyanin tif hoa dau biec bang dung m6i con vdi ti 18 nguyen H6u: dung moi la 147/1 (ml/g) tai 59''C va trong thdi gian chiet la 105 phdt thu dUdc h^m lifdng anthocyanin la 8,4051 (mg/g).
Tiif khoa: Anthocyanin, hoa dau bi6'c, Clitoria Ternantea L.
Hoa dau biec (Dau biec lam) co ten khoa hoc !a Clitoria ternatea. cay than thao, day leo, co nguon goc tif vung Caribe, mien Trung My va Mexico, sau dd difdc trong nhieu d An Do de iJng dung vao y hoc cd truyen (Mukherjee va cong si^, 2008). Hoa dau bi6'c co mau xanh du'dng, sac to hoa mau xanh difdc siJ dung theo truyen thd'ng nhif chat mau thifc pham. Hoa dau biec chifa anthocyanin cd mau xanh difdng, cd tiem nang du'dc sijf dung lam chat mau xanh tif nhien cho thifc pham, my pham va difdc pham d pH trung tinh [6]. Anthocyanin trong hoa dau biec gom nhieu nhom cd ban va difdc xac djnh theo mau s5c: pelargonidin la mau cam, cyanidin cho mau do, delphinidin cho mau xanh [2].
Anthocyanins la polyhydroxy glycosy! hoa va cac dan xua't cua 2-polymethoxy phenylbenzopyryiium cation, td'e la cac cation flavylium [3]. Anthocyanin la cha't chong oxi hda, ddng mot vai trd quan trpng
trong vi$c ngan ngij'a cac benh tim mach, than kinh, ung thu va tieu difdng... [4] Athocyanin khong co dpc tinh va khong cd ba't ki gidi han to'i da nao cho cac u'ng dung trong thifc pham. Tuy nhien, anthocyanin khong on dinh va rat d l bi thoai hoa b6i cac ye'u to nhif pH, nhiet dd lifu trij, ca'u true hda hpc, n6ng do, anh sang, oxy, dung moi, sd hien dien cua enzyme, favonoid, protein va ion kim loai [7]. Muc tieu^cua nghien cUu la khao sat cac diiu kien td'i u^ de trich ly anthocyanin tiJ' hoa dau biec nham l?ng dung trong thifc pham.
2. Doi tifging va phittAig phap nghidn cifu 2.1. Nguyen lieu, hoa chat vd thiet bi Nguyen lieu: Hoa dau biec (Clitoria Ternatea L.) du'dc trong d tinh Tra Vinh. Hoa dau bi6'c vHa.
nd, cdn tUdi, canh hoa cang min, mau xanh du'dng sam. Hoa sau khi thu hoach du'dc van chuyen va su- dung trong vdng 48 gid vdi dieu kien bao quan lanh va 24 gid ct dieu kien binh thifdng.
HOA HOC-CONG NGHE THirc PHAM
Hoa chat: Dextrin (Phap); kali clorua, acid clohydric, natri acetat, ethanol, methanol, kali iotdua, iot, sat clorua, acid sunfuric, acid acetic, chloroform, natri hydroxit, anhydride acetic, i-a naphthol, nhom Clorua, sodium nitrite, natri cacbonat, maltodextrin va gelatin (Trung Quoc).
Thiet bi: Co quay chan khdng Stuart RE300B (Cole Parmer, My), tu say memmert UNB 40 (Memmert, DiJc), tu am memmert INE 500 (Memmert, Di'fc), be dieu nhiet memmert WNB. 14 (Memmert, Dilc), may do pH S220 (Mettler Toledo, Thuy Sy), may nghien biia MF 10 B (IKA, DiJc), may quang phd UV-Vis Genesys 20 (Thermo Scientifi Geneys, My).
2.2. Phi&fngphdp thi nghiem
Nguyen lieu hoa dau biec sau khi lam sach difdc xac dinh do am va dp tro lan lifdt theo TCVN 1867:2001 va TCVN-5253-90; Xac dinh ham lifdng chat tan bSng each la'y 10 ml dich chiet bang nifdc va 10 ml dich chiet bang methanol 60% dem di say de'n khd'i lifdng khdng ddi d 75"C, sau dd dem can xac dinh ham lu'dng cha't tan thu difdc; Xac dinh ham lu'dng flavonoid, terpenoids, dinh tfnh alkaloids, tannm, glycosides, resins, flavonoid, sterol, saponin, carbohydrates, phenol, terpenoids trong nguyen liSu hoa dau biec [8]; nguyen lieu hoa dau biec du'dc do mau Lab su' dung Chroma Meter. San pham difdc danh gia boat tinh khang oxy hda bang phifdng phap ABTS [9].
Xdc dinh hdm lupng Anthocyanin theo phuong phdp pH vi sai: Ham lu'dng anthocyanin difdc xac dinh theo phu'dng phap pH vi sai va du'dc tinh quy theo cyanidin-3-glucoside. Cyanidin-3-glucoside du'dc chon, vi day la dang phd bien cua anthocyanin trong tif nhien [10], Phifdng phap pH vi sai difa vao sif chuyen ddi thanh cac ca'u tnic khac nhau theo pH cua cac sac to' anthocyanin va the hien ro qua pho hap thu khac nhau tu'dng u'ng.
Dang oxonium cd mau tdn tai 5 pH i.O va dang hemiketal khdng mau 6 pH 4.5. MSu difdc pha loang trong 2 dung dich dem: dem kali clorua 0,025 M (pH 1.0) va dem natri axetal 0,4 M (pH 4.5). Tat ca cac phep do dp hap thu bang may do quang pho du'pc thifc hien d bu'dc sdng 547 nm (la bifdc sdng cifc dai cua anthocyanin do difpc) va 700 nm. Xac dinh anthocyanin theo cdng thu'c sau:
^_AxMWxDF\1000 V
Trong do: A la dp hap thu cua anthocyanin vdi:
'^imnm>\iW ] 0
s \l m!^J100-w))iW--
~ ('^Avisraax" •^7O0nmJpH45
MW = 449.2 g/mol la khd'i lifdng phan ttf cua cyanidin-3-glucosdie, DF la dp pha loang; V la the tich dich ehiet; I la be day cuvet (cm); e = 26900 la he so' hap thu phan tu' cua cyanidin-3-glucosdie (l.mol'.cm-'); ra^L la khd'i lifdng nguyen heu (g);
w la am dO nguyen lieu; a la lifdng anthocyanin (g).
2.3. Phidfngphdp thifc hi$n
Hoa dau biec sau khi thu hoach .se du'pc rSa sach, cat phan canh hoa mau xanh sSm bo dai hoa mau xanh la va nhuy hoa, sau do dem say bang phifdng phap sa'y khay d 55"C, thdi gian say khac de dat am dp mong mudn cho tifng thi nghiem.
Nguyen lieu sau khi say kho tie'p tuc du'dc nghien vdi cac kich thu'dc khac nhau bang may xay. Bot hoa difpc difng trong tui zip hut chan khong, cd chu'a tiii hut am sihcagel va du'dc bao quan trong tu lanh ZO^C. Nguyen li6u difdc ngam vdi dung mdi thich hdp va thay doi cac dieu kien nhiet dp, thdi gian chiet, ty le dung moi dd dat difpc ham lu'png anthocyanin cao nhS^t. Dich sau khi chiet du'pc loc qua gia'y loc de loai bo ba va bdt hoa sau khi ngam nham thu difdc dich trong sau do mang co quay. Co quay chan khong cf 50"C nhSm dudi dung moi tdn dif va thu pham mau anthocyanin d dang co dac.
Cao chie't sau khi cd quay difdc dinh mifc lai vdi nifdc de ddng nhat the lich khi do ham lifdng anthocyanin.
2.4. Bo tri thi nghiem
2.4.1. Khdo sdt thdnh phdn. ddc tinh hoa ly cua nguyen lieu
Nguyen lieu hoa dau biec difdc phan tich cac chi sd dp am, do tro, xac dinh ham lifdng chat tan, flavonoid, terpenoids, do mau lab va dinh tinh thanh phan alkaloids, tannin, glycosides, resins, flavonoid, sterol, saponin, carbohydrates, phenol, terpenoids.
2.4.2. Khdo sdt dnh hudng cua cdc diiu kien trich ly den hdm lUpng anthocyanin
Thi nghiem sd bp khao sat anh hifdng cua cac dieu kien trich ly difdc bd tri thi nghiem 1 ye'u id hoan toan nglu nhien kieu luan phien tid'ng bien theo thiJ tif la loai dung mdi (ethanol 60", eflianol 60" va HCl 1%, nifdc, nifdc va HCl 1%), am do nguyen lieu (nguyen li6u tifdi, 41.31, 15.38, 12.24%), b'ch thu'dc nguyen li$u (0,5 - 0,9 mm, 1 - 2 mm, 3 - 5 mm, nguyen canh), tl le dung moi/nguyen
TAP CHi CONG THKfiNG
lieu (50:1, 100:1, 150:1, 200:1 mVg) va mdt thi nghiem 2 ye'u td' la thdi gian chiet (Ih, 2h, 3h) va nhiet dp chie't (nhiet dp phong, SO^C, eO^C, 70"C).
Ket qua lifa chpn khi khao sat cac ye'u to'tru'dc du'dc s^ dling de khao sat cho ye'u to sau. Yeu td cd dinh cho thi nghidm khao sat anh hifdng cua loai dung moi 1& nguyen liSu tifdi, hoa nguyen canh, ti le nguyen lieu/dung mdi (50:1, ml/g), thdi gian chie't 1 h tai nhiet dp 50°C. Chi tieu theo doi la ham lifdng anthocyanin. Sau khi xac dinh tam thdng qua cac thi nghiem tham dd, 3 ye'u to ty le dung moi/nguyen lieu, nhiet dp chie't va thdi gian chiet difdc bd tri theo kieu true quay tam (Rotatable Central Composite Design) de xac dinh anh hu'dng cua cac ye'u td va tdi u\i hda quy trinh chiet anthocyanin bang phu'dng phap dap u'ng be mat RSM.
2.5. XiJtly sd'lieu
Moi thi nghiem difdc thifc hien lap lai 3 lan, difpc tinh toan va ve do thi bang phan mem Microsoft® Office Excel 2013. Phan tich thdng ke ANOVA va trac nghiem LSD du'dc su" dung de so sanh anh hifdng cua cac yeu td', difdc xijf ly bang phan mem Statgraphics Centurion XV 9 (Statgraphics Technologies, Inc., USA) va sif hd trp cua phan mem thd'ng ke JMP 10. Do tm cay 95%
difdc ap dung cho ta't ca cac phep thdng ke.
3. Ket qua va thao luan
3.1. Khdo sdt thdnh phdn, ddc tinh hda ly cua nguyen lieu
Nguyen lieu cd do am la 89,67 ± 0,478%, dp iro la 0,5903 ± 0,0431% cho tha'y, lifdng nifdc cdn trong hoa la kha nhieu, nen dl bi dap nat va hif hai.
Can bao quan hoa lu'di d tu lanh du'di 4°C hoac co the sa'y khd tao bot de bao quan lau dai. Khi dinh tinh cac thanh phan trong nguyen lieu cho tha'y sif hien dien cua ankaloids, tanms, glycosides, carbonhydrat, terpenoid va flavonoid trong hoa dau
biec. Flavonoid va tanins la hai hdp chat chdng oxy hda va tao mau xanh cho hoa, carbonhydrat dam nhan vai trd nhif thanh te bao, glycosides trong hoa dau biec cho dia'y sif cd mat ciia difdng, tao vi ngot cho hoa. Ham lu'dng flavonoid, teqienoids, chat tan trong mSu chie't vdi nifdc lan lu:dt la 15.74 mg/g (tifdng difdng khoang 1.57%), 48.18 mg/lOOg (tu'dng du'dng khoang 0.048%), 0.584 g/g chat kho.
Ket qua do mau Lab cho tha'y phan canh hoa tren CO mau sam, dd sang tha'p hdn phan cud'ng canh hoa du'di, phan canh hoa tren cd sif xua't hien cua mau do va mau xanh difdng, cdn phan cudng canh hoa difdi khdng cd mat cua mau do va mau vang.
Khi thay ddi pH, nhan tha'y mau sSc ciia dich chiet thay ddi (Hinh 1): d pH tha'p (pH = 1.0 va pH = 2.0) thi anthocyanin ben va cd mau do; khi pH tang len (pH = 3-5) thi cac anthocyanin kem ben va cd mau tim do anthocyanin tdn tai du'di dang base quinon, khi pH = 6 - 8 thi anthocyanin tdn tai du'di dang quinoidal anhydro nen co mau xanh dam; khi pH
= 9 - 11 thi cac anthocyanin co sif chuyen ddi ti!f xanh dam sang vang tao nen mau xanh la, anthocyanin cd mau vang va dam dan d pH = 12 - 14 vi anthocyanin ton tai du'di dang chalcon.
3.2. Anh hi^ng cua dieu kien trich ly den hdm ItMng anthocyanin
Hinh 2 cho tha'y ham lu'dng anthocyanin trong nguyen Ueu cua dung mdi nu'dc la cao nha't la 4.2248 (mg/g db), do nifdc cd sif phan cifc manh nen hda tan hoan toan anthocyanin. NhJJng dung mdi con lai, mot phan la do chifa hda tan het ham lifdng vao dung mdi, mot phan la do siJ anh hifdng ciia loai dung mdi de'n nguyen lieu lam giam ham lu'dng anthocyanin xud'ng dang ke. Ke't qua ANOVA vdi P = 0.002 (< 0.05) cho tha'y sif anh hifdng cua cac loai dung mdi len ham lu'png anthocyanin vdi dp tin CEly 95%. Ket qud LSD chi
Hinh 1: Mdu sdc anthocyanin cija hoa ddu biec d moi trUdng pHtU 1 - 14
HOA HOC-CONtNtHETHIfC PHAM
Hinh 2: Anh hUSng cua loai dung moi len ham iiHSng anthocyanin
m lirtfng yanin (mg/g db)
4.2248 2 9056 • 2 5048
Ethanol Ethanol Nuac Nudc va 60 do 60 dova HCl 1%
HQ 1%
Dung moi
Hinh 4: Anh hifdng cua file dung mdi:
nguydn lieu len ham lifdng anthocyanin
50/1 100/1 150/1 200/1 Tl le dung moi/ nguyen lieu
Hinh 3: Anh hUdng cua dm dd len ham IUdng anthocyanin
1 en
^ 0.8 f - 06 5 I f 0-4
I I »-^
•S 0 e
0 0
0 0 0 0 Am do (%)
0 0
Hinh 5: Anh hUdng cua kich thUdc nguyen lieu l§n hdm IUdng anthocyanin
10 n
•yi 8 •
§ . - « -
^ 1 f 4 -
7.7316
6.6462 J 6,2287 3.5068 I I I
• I I I
a Nguyen 3 - 5 1-2 0,5-0,9 canh mm mm mm
Kich thirtfc nguyen lieu
ra sif khac biet giiJa nifdc vdi cac loai dung mdi con lai. Vl vay, chon nu'dc Iam dung mdi cho cac thi nghiem tiep theo. Su' dung nu'dc con an toan vd mat moi tnfdng, an toan khi su' dung trong thu'c pham, giam chi phi dudi dung mdi sau qua trinh chie't.
Hinh 3 the hien ham lu'dng anthocyanin tang khi am dp giam ti^ 89.66% xud'ng 15.38% nhifng den 2m do 12.24 % thi ham lu'dng lai giam do thdi gian say keo dai, nhidt dp va khdng khi se lam anthocyanin bi oxy hoa nen hao hut ham lu'dng anthocyanin trong nguyen lieu. Mac khac, thdi gian say ngan thi am do nguyen li6u cao dan den viec hu"
hdng trong qua trinh bao quan lam giam ham lifdng andiocyanin trong nguyen hdu. Ket qua thdng ke cho tha'y, am dp cd anh hifdng de'n ham lu'dng anthocyanin d dp tin cay 95%. Khi xet ket qua LSD cd SLf khac biet d 4 Im do khac nhau nen chpn am do 15.38 % de tranh di sif hao hut ve ham lifdng anthocyanin va la am do tdi u'u cho cac thi nghiem tie'p theo.
Hinh 4 cho tha'y khi tang ti le dung mdi/ nguyen lieu tif 50:1 den 100:1 Uii ham lifpng anthocyanin
tang, vi lu'dng dung mdi qua it khdng du dd hoa tan cac cha't trong nguyen lieu, dac biet la anthocyanin.
Khi ti Id dung mdi/ nguyen Heu la 150:1 thi lifdng chat tan trong nguyen lieu tang, ham lu'dng anthocyanin dat gla tn cao nha't nhtfng khi tang ti Id thanh 200:1 thi ham lUdng anthocyanin lai giam do dung mdi qua nhieu ma cha't tan trong nguyen lieu da bao hda nen khdng the tang them. Va khi cang tang dung mdi, ham lifdng anthocyanin Iai cang giam. Ket qua thd'ng ke ANOVA vdi P = 0.005 (< 0.05) nen co sif anh hifdng cua ti Id dung mdi dd'n ham lu'dng anthocyanin cua nguyen lidu d dp tin cay 95 %. Ket qua LSD cho thay sif khac bidt giffa ti te 150:1 vdi cacti Id dung mdi/nguyen lidu con lai.
Do dd, ti le 150.1 la phii hpp nha't de khdng lam tdn tha't dung mdi.
^ Hinh 5 cho tha'y ham lifdng anthocyanin khi ta dd' nguyen canh la tha'p nha't vdi 3.5068 (mg/g db) va cao nha't dat 7.7316 (mg/g db) khi nguyen lieu nghien xud'ng con khoang 1 - 2 mm. Khi kich thifdc qua to, thanh nguydn lieu chu'a bi pha huy, dung mdi se rat khd de tham vao nguyen lieu!
lAP CHl CONG THlfdNG
ddng thdi gay cin trd cho viec khuech tan cua anthocyanin vao dung mdi. Ngifpc lai, nd'u nguyen lieu dddc nghien kich thu'dc nhd hdn td'e be mat tie'p xue Idn hdn ddng thdi thanh te' bao bi pha hiiy nen de xam nhap vA khue'ch tan anthocyanin vao dung mdi hdn Tuy nhien, khi thay dpi kich thifdc nguydn heu cdn khoang 0.5- 0.9 mm thi hhm lifdng anthocyanin lai giam vi khi nghien qua min, ham lifdng se ma't di trong qua trinh nghien, nguyen lieu qua min se bi bim dinh lai vdi nhau trong qua trlnh chid't, gSy khd khan trong vide tham tha'u va khud'ch tan anthocyanin trong dung mdi vao nguydn hdu; Khi tach dung mdi lai tao thanh cac mang bao giiJ nifdc lai phia trong gSy can trd cho vide tach dung mdi. Kd't qua ANOVA the hien sif anh hu'dng cila kich thifdc nguydn hdu len ham lifdng anthocyanin cua nguyen lieu vdi dd tm cay 95%. Bang LSD ta thS'y sd khac biet kich thu'dc nguyen hdu 1 - 2 mm so vdi cac kich thifdc cdn lai. Vi vay, kich thu'dc nguyen lieu khoang 1-2 mm la
phii hdp cho qua trinh chiet ciia cac thi nghidm tid'p theo.
Hinh 6 cho tha'y khi tang thdi gian chid't tiJf 1 gid dd'n 2 gid d ca 4 nhiet do, ham lifdng anthocyanin tang. Khi thdi gian chiet la 3 gid, ham lifdng anthocyanin giam. Vdi nhiet dp, khi tang nhiet dd tiJf nhiet do phdng len dO'^C, ham lu'dng anthocyanin tang cao nha't va giam khi nhiet dp la 70''C. d thdi gian vk nhiet do thich hdp se giup dung mdi hda tan cac chat trong nguyen lieu dan dd'n ham lu'dng anthocyanin trong nguydn lieu tang. Tuy nhidn, ham Iifpng anthocyanin se giam dang ke ne'u thdi gian keo dai qua miJc va nhidt dd cao. Thdi gian qua ngan hay qua dai cung khdng thu difdc ham lifdng anthocyanin cao, vi trong thdi gian ngan anthocyanin chifa the hda tan hd't vao trong dung mdi trich ly difdc;
cdn trong thdi gian dai d nhidt dp cao, anthocyanin kem bdn vdi nhiet dp va cd the bi phan huy.
Ke't qua ANOVA cho tha'y, thdi
gian va nhidt dd chid't deu anh hu'dng len ham lifdng anthocyanin vdi miJc tin cay 95%. Hai yd'u td nay cd sif tifdng tac vdi nhau. Bang LSD vd thdi gian va nhiet do lin lu'di cho dia'y 2 gid, dO^C khac biet so vdi cac thdi gian va nhiet do cdn lai. Vay, thdi gian 2 gid vS nhiet dd 60"C du'dc chpn de thifc Men cac thi nghiem tie'p theo.
Difa vao kd't qua cac thi nghiem khao sat sd bo cac ye'u td anh hu'dng dd'n ham lifdng anthocyanin, ta chpn tSm nhif sau: ti le dung mdi: nguyen lieu:
150.1 (v:w), nhidt dd chid't: 60°C, thdi gian chiet:
2h. Tif cac yd'u td' da chon, ma tran thi nghidm td'i lfu difdc thie'tlap theo kieu quay tam nhif Bang 1.
Hinh 7, ta tha'y P = 0.0021 (< 0.05) va RSq = 0.97 (> 0.8) cd nghia la md hinh dif doan tuyd'n tinh du'dc xem la td't, phii hdp dd suT dung. Do do, phifdng trinh dd doan ham lu'dng anthocyanin theo cac dieu kien chie't la:
Y = 8,4704 - 0,41Xi2 - 1,078X22" 0,494X32 - 0,242X,X2 - 0,267X2X3 - 0,231X2 - 0*26X3
Hinh 6: Anh hUdng cua thdi gian vd nhiet do chief len hdm IUdng anthocyanin
6.1786 6.128:
5 5941]
' Nluet dg phong Thdi gian (li)
• 50 do C "eOdpC Bang 1. Bdng ma hoa bien trong to! Uu
^ " ^ ^ - ^ ^ ^ ^ Bi§'n
Ti le dung m6i/ nguyen lieu (v/w)
Nhiet do ell let (»C)
Thdi gian chiet {gic3)
•1
100
50
1 0
150
60
2 +1
200
70
3
HOA HOC-CONGNGHETHtfCPHAM
HInh 7: 00 thi hdm lifdng anthocyanin da kien
5,5 6 6,5 7 7,5 8 3,5 Ham luong anthocyanin Predicted P=0,0021
RSq=0,97 RMSE=0,2454
Bang 2. Bdng doi chieu diiu l<ien toi Uu va thi/c te
"iSito Dung moi (v/w)
Ntiletdopc) Tii6i gian (ii) Ham luay anthocyanin (mg/g db)
Ly thuyet 146,707 69,317 1,748 8.5126
Thllcl*
147 59 1,75 S,40S1± 0.0126
Trong do: x^ la nhiet do ("C); x, la ti le; X3 la thdi gian (phiit).
Phifdng tnnh h6i quy nay difdc diing nhif mdt md hinh de dif doan difdc ham lu'dng anthocyanin vdi cac diiu kidn nhidt do, ti le va thdi gian tu'dng u'ng. (Bang 2)
Tie'n hanh thifc nghiem chid't vdi diiu kidn td'i itu, thu difdc ham lifdng anthocyanin trung binh la 8,4051 ± 0,0126 (mg/g db), sai so be hdn 5% so vdi ham lu'dng anthocyanin theo ly thuye't. Dieu nay ehu'ng to md hinh nay co y nghTa va phu hdp dd' tach chie't anthocyanin trong dau bide.
4. Ke't luan
Dieu kien thich hdp de trich ly dich mau chu'a anthocyanin bang phifdng phap ngSm chid't nhif sau: Dung mdi chie't la nu'dc, kich thu'dc nguyen lieu khoang 1 mm, ty le dung moi/ nguyen lidu la 147:1 (ml/g) vdi thdi gian ngam chie't 105 phiit d nhiet do chie't 59''C. Ham lu'dng anthocyanin trong dich chie't khoang 8.4051 (mg/g) •
TAI LIEU THAM KHAO:
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2. Tri Nhut Pham et al (2019). Extraction of anthocyanins from Butterfly pea (Clitoria ternatea LFlowers) in Southern Vietnam. Response surface modeling foroptimization of the operation conditions.
3 Brouillard R. lacobucci GA, Sweeny JG. (1982). Chemistry of anthocyanin pigm.enls. 9. UV-visible spectrophotometric determination of the acidity constants of apigeninidin and three related 3-deoxyflavylium salts. J Am Chem Soc. 1982;104(26):7585 - 7590.
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5. Inc TF.S (2016). Fast HPLC Analysis of Dyes in Foods and Beverages
6 Ue P.M.. Abdullah R., and Hung L K. (20!l).Thermal Degradation of Blue Anthocyanin Ewaet of Clitoria ternatea Flower. 2ml International Conference on Biotechnology and Food Science.
7. Rein, M. (2005). Copigmentation reactions and color .stability of beriy anthocyamns.Helsinki: University of Helsinki pp. 10 -14.
TAP CHi CONG THIftfNG
8. Sriyela Chakraborty, Souvagyalaxmi Sahoo. Bhagat A., et al (2017), Studies On Antimicrobial Activity, Phytochemical Screening Tests. Biochemical Evaluation OfClitorea Ternatea Linn. Plant Extracts, Zenodo.
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10 Ly Hoang VU (2009) - Khdo sdt qud Irinh trich ly vd do bin mau Anthocyanin td dai hoa bup gidm Hibiscus Sabdariff'a L. Dai hoc Nong Lam TP. Ho Chi Minh. 117.
Ngay nhan bai: 19/10/2019
Ngay phan bien danh gia va suTa chffa: 29/10/2019 Ngay cha'p nh§n dang bai: 9/11/2019
Thong tin tdc gid.
1. TS. MAI HUYNH CANG 2. D6 THI THUY TIEN 3. NGUYEN THI TU
Bo mon Cdng nghe Hoa hoc - Trrfcfng Dai hpc Ndng LSm TP. Ho Chi Minh
OPTIMIZATION EXTRACTION CONDITION OF ANTHOCYANIN FROM BUTTERFLY PEA FLOWER
(Clitoria Ternatean L.)
• DR. MAI HUYNH CANG
• DO THI THUY TIEN
• NGUYEN THI TU
Department of Chemical engheering a n d processing Nong Lom University of Ho Chi IVIinhi City ABSTRACT:
In this study, optimisation conditions for anthocyanin extraction from Butterfly Pea Flower (Clttoi-ia Ternaiuea L.) was carried out. Firstly, Butterfly Pea Flower were evaluated the physicochemical properties including ash content, moisture content. The optimum conditions for extraction of anthocyanin from Butterfly Pea Flower using ethanol were as follows: the ratio of solvent/ material of 147/1 (ml/g), extraction temperature of 59°C and extraction time of 105 minutes. The optimum yield extraction of anthocyanin was 8.4051 (mg/g).
Keywords: Anthocyanin, Butterfly Pea Flower, Clitoria Ternantea L.
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