KHOA HOC KY THUAT THU Y TAP XXVI SO 6 - 2019
PHAN LAP, D|NH DANH MOT SO VI K H O A N G A Y BENH HO HAP TREN HEO
Dgng Thi Xudn Thiep, BHi Nguyin Hodng Trang, Le Thanh TUng, Vo Thi Trd An Khoa Chdn nuoi Thu y, Truang Dgi hoc Nong Ldm Tp.HCM
TOM TAT
Nghien cuu nay nhim muc dich phan lap, dinh danh cac vi khuSn gay benh hd hSp gdm:
Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida va Bordetella bronchiseptica tir mau dich miii va mSu phdi cua heo cd bieu hien benh hd hip.
Kgt qua nghign cim cho thSy, trong tdng sd 130 mhx dich mui va mSu phdi, da phan lap va dinh danh dupe 42 yi khuin cuaca 4 ioai vi khuSn, chi6m ty le 32,3%. Ty le phan lap Haemophilus parasuis la cao nhat, chigm 11,5%, tigp den Xk Pasteurella multocida vdi ty le 9,2 %; Actinobacillus pleuropneumoniae, Bordetella bronchiseptica cd ty le phan lap lan lugt la 6,9 % va 4,6%. Ty le cac vi khuSn phan lap dugc tir ph6i (17,7%) cao hon so vdi dich mui (14,6%) trgn t6ng sd mlu khao sat.
Tic khod: Phan lap, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida, Bordetella bronchiseptica, heo.
Isolation and identification of some respiratory pathogens from swine Dang Thi Xuan Thiep, Bui Nguyen Hoang Trang,
Le Thanh Tung, Vo Thi Tra An
SUMMARY
This study was conducted to isolate and identify some respiratory pathogens including Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida and Bordetella bronchiseptica based on testing the nasal fluid and lung samples from swine with respiratory disease symptoms. The studied results showed that from a total of 130 pig nasal fluid and lung samples, 42 isolates of ali 4 types of bacteria were isolated and identified, accounting for 32.3%. The isolation rate of Haemophilus parasuis, Pasteurella multocida, Actinobacillus pleuropneumoniae and Bordetella bronchiseptica was 11.5%, 6.9%, 9.2% and 4.6%, respectively.
The isolated bacteria rate from the lung and nasal fluid samples was 17.7%o and 14.6%, respectively.
Keywords: Isolation, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida, Bordetella bronchiseptica, swine.
I. DAT V A N D E pleuropneumoniae la vi khuan dimg thu hai n - - , . , i , • .. X , , ,. . eay viem phdi-mang phdi. Mdt vi khuan khac Benh ho hap do vi khuan tren heo la raot ^ •' . ^, . , ,- ,, , -, nguyen nhan quan trong lam gia tang ty le benh 8=-^ " ™ «" " " " « ' ^ ^ b^o h^HaemopMrn vi ty ie ChSt tren heo, din d6n t6n that kinh t6 f''"'-"' C" ">"«,™ '''• ^O'^)- Quan trotig cho trang trai ehan nuoi. V. khu^n gay benh ho hon, nhimg vi kltuan gay benl, nay con la yeu hip quan trong nhJt la Pasteurelh multocida. t6 mo dirtag cho nhung tac nhan gay benli ho Vi khuSn nay thirdng gay benh k6t hgp v6i mot hap khac nhu benh ciim, benh do Mycoplasma,
^HAnia khic ]k Bordetella bronchiseplica gay roi loan ho hip sinh san (Porcine respiratory viem teo xoang mui tren heo. Actinobacillus reproduction syndrome-PRRS) hay hoi chumg
KHOA HOC KY THUAT THTJ Y TAP XXVI SO 6 - 2019
coi cgc tren heo cai sira (Porcine circovirus type 2- PCV2). Tat ca nhung tac nhan nay cd moi lien he mat thilt vdi nhau, chimg tao ngn nhimg mat xich quan trgng trong viec tao ra benh ly hd hap tren heo (Sassu va ctv, 20] 8). Trong sd cac bai bao tren Tap chi Khoa hgc ky thuat thii y tinh tii thdi dilm nam 2010-2017, chua c6 cdng bd nao phan lap, dinh danh cac vi khuan gay benh ho hap tren heo tai khu vuc mien Dong Nam Bg. Nghien cim cua chiing tdi dugc thuc hien vdi muc tieu phan lap, dinh danh cac vi khuan gay benh hd hap tren heo bao gom Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida va Bordetella bronchiseptica tir cac man benh pham thu thap tren heo tai mgt so trai va 16 mo thudc khu vuc nay. Kgt qua nghien cdu gop phan quan trgng, lam co sd cho viec chan doan nguyen nhan gay benh ho hap do vi khuan tren heo tai cac khu vuc da lay raau, tir dd, djnh hudng cho viec dieu tri benh ciing nhu thuc hien cac nghien ciiu tiep theo.
n. NOI DUNG VA PHLTONG PHAP
NGHIEN ctru
2.1. Noi duDg
Phan lap va dinh danh cac vi khuan gay benh ho hap tren heo bao gom Actinobacillus pleuropneumoniae, Haemophilus parasuis.
Pasteurella multocida va Bordetella bronchiseptica tu mau dich miii va mau phdi cua heo cd bieu hien benh hd hap.
2.2. FhiroTig phap ngbien cuu 2.2.1. Thu thgp mau
Tong so 80 mau dich miii heo dugc thu thap tii 8
trai heo thuoc tinh D6ng Nai va 50 mau phdi dugc thu thap tai 1 16 ra6 heo thugc tinh Binh Duang.
MSu dich mui dugc thu thap tren nhdng heo cd bi6u hien benh ho hip nhu s6t, ho, chay djch mui, thd ng5i, thd thi bung, da tim tai. Dung tam bdng vo trung ngoay sau vao ben trong miji heo, sau do be ngan tam bong va cho vao eppendorff vd triing.
MIU phoi cd cac benh tich nhu xuat huyet, hoai tit, hay vigm phdi dinh sudn se dugc thu thap ya de rieng tung mau trong cac tui sinh hgc. Cac man sau khi thu thap dugc cho vao thiing dung mau, bao quan bang da khd va van chuyen ve phdng thi nghiem trong vdng 12 gid de tien hanh phan lap, dinh danh.
2.2.2. Phan lap
Quy trinh phan lap dugc thuc hien theo cong bd ciia Zhao va cgng su (2010). Mdi trudng phan lap cac vi khuan tren la mdi trudng TSA (Tryptose soy agar; Oxoid) bo sung 5% huyet thanh bd (Fetal bovme serum; Difco) va 10 (xg/ml nicotinamide adenine dinucleotide (NAD; Merck). Ddi vdi mau dich mui, mau tam bdng ngoay dich mui se phet true tiep d mgt gdc tren dia, sau do dung que cay de ria nhimg dudng cay cdn lai. Ddi vdi mau phoi, be mat phoi dugc dot bang cdo, sau do diing keo da dugc dot trgn den cdn cat sau xuong phan mo phoi ben trong, dong thdi cat lay mdt mau phoi nho va phgt Ien dia, tiep tue cay ria bang que cay.
Cac dia nuoi cay vi khuan dugc u higu khi d 37°C trong 24-48 gid. Sau thdi gian nudi cay, viec nhan dien va phan Ioai 4 vi khuan muc tigu tren dua vao hinh thai khuan lac, ket qua nhugm Gram va phan ling catalase dugc mo ta trong bang 1.
Bang 1. C<y so" phan loai vi khuan Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida va Bordetella bronchiseptica sau khi phan lap
Loai vi khuan A. pleuropneumoniae Haemophilus parasuis Pasteurella multocida Bordetella bronchiseptica
Tho'i gian u
<24h/ 37''C (20 gid)
>24h/ 37'C (40 gid}
24h/ ZVC 24h/ 37°C
Kich CO' nho rit nho ldn
'••^
Mau s i c , do due trang due trang due trang dgc t r a n n tr o na
Phan O'ng catalase
*
+ +
Hinh thai C4u tare, nho, Gram am Cau true hoac sgi dai. Gram am Cau true, Gram am '--BLl ifU'C,
KHOA HOC KY THUAT THtJ Y TAP XXVI SO 6-2019
Cac khuan lac cd ket qua phan loai nhu nhau hr ciing mgt dia nudi cay se dugc tmh la raot vi khuan.
Vl khuan nay se tigp tue dugc dinh danh bSng PCR.
2.2.3. Dinh danh bdng PCR
Primer su dung trong dinh danh Actinobacillus pleuropneumoniae, Haemophilus parasuis, Pasteurella multocida va Bordetella bronchiseptica dugc trinh bay d bang 2.
Bang 2. Primer SIF dung cho djnh danh cac vi khuan
Vi khuan Trinh ty (5'-3') Kich
CO- (bp) TLTK Actinobacillus
pleuropneumoniae Haemophilus parasuis Pasteurella multocida Bordetella bronchiseptica
apxIVA 16SrRNA
toxA fla
AP-IVF AP-IVR HPS-1 HPS-2 KMT1 T7 KMT1 SP6
Fla4 Fla2
ATACGGTTAATGGCGGTAATGG ACC TGA GTG CTC ACC AAC G GTG ATG AGG AAG GGT GGT GT GGC TTC GTG ACC CTC TGT ATCCGCTATTTACCCAGTGG GCTGTAAACGAACTCGCCAC TGGCGCCTGCCCTATC AGGCTCCCAAGAGAGAAA
Hrici'nova va ctv (2010) Hncinova va
ctv (2010) Hricinova va
ctv (2010) Zhao va ctv (2011)
Viec dinh danh vi khuan bang ky thuat PCR dua trgn phat hien cac vi khuan cd mang gen I6S rRNA hoac gen doc td (apxlVA, toxA, fla) de chac chan cac vi khuan phan lap dugc la cac VI khuan muc tieu cua nghign cuu.
Ly hich DNA: DNA cua vi khuan dugc ly hich bang phuang phap sdc nhiet. Dung que cay lay 3 - 5 khuan lac da dugc cay thuan tren mdi trudng TSA cho vao dng eppendorff cd chiia sin SOfil TBE (his - boric EDTA), tigp theo dat eppendorff vao nude dang dun sdi 100°C/5 philt, sau do cho vao hi -70''C/5 phut. Sau cung, Iy tam dich chigt DNA d miic 15.000 vdng/phiit trong 5 phut, hiit lay 20pl phan nude ndi trgn be mat eppendorff cho vao mdt dng eppendorff khac va bao quan d -20''C.
The tich I phan ung PCR la 20pl, bao gdm 10^1 Go Taq green (Promega, My), 2pl primer (Promega), 2\L\ DNA mlu va 6 ^l H^O (nuclease free water) vdi quy trinh nhiet theo cac tai lieu tham khao d bang 2.
Dien di va dgc kgt qua: 5 ^il san pham PCR se dugc dien di tren gel 1,5% agarose (Promega, My) CO chiia GelRed (Biotium, My) trong mdi mrdng TBE 0,5X, thdi gian dien di la 20 phut d hieu dien thg 150 volt, cudng do 144 mA.
Kgt qua dugc dgc tren may Biorad UV2000.
Su dung thang chuan DNA phu hgp de udc lugng kich thudc doan san pham PCR. Cac vi khuan Actinobacillus pleuropneumoniae ATCC 27090, Pasteurella multocida ATCC 12945, rigng cac gdc Haemophilus parasuis, Bordetella bronchiseptica sau khi PCR da dugc giri giai trinh tai cdng ty TNHH Thuang mai va dich vu Nam Khoa, Tp.HCM va su dung Iam ddi chiing duang cho dinh danh cac vi khuan tiep theo.
m . KET QUA VA THAO LUAN Qua nghien cuu, chung toi da phan lap dugc 42 vi khuan, ty le phan lap timg loai vi khuan dugc trinh bay trong bang 3.
Kgt qua tir bang 3 cho thay ty le phan lap cac vi khuan gay benh hd h4p trgn tdng sd mhi khao sat la 32,3%. Trong do, vi khuAn Haemophilus parasuis cd ty le phan lap cao nhat d ca 2 mlu chigm 11,5%; tigp dgn la Pasteurella multocida, Actinobacillus pleuropneumoniae va Bordetella bronchiseptica cd ty le phan lap Ian lugt la 9,2%; 6,9% va 4,6%. Ngoai ra, ty le cac vi khu§n phan lap dugc tir phdi (17,7%) cao hem so vdi dich miii (14,6%) tren tdng sd mSu khao sat. Do dd, ty le phan lap timg loai vi khuan tren m§u phdi ciing cao ban so vdi man dich rtr miii.
KHOA HOC KT THUAT THU Y TAP XXVI SO 6 - 2 0 1 9
Djch mui Phoi
Bang 3. Ty le phan lap
App 80 4 50 5 Tong so mlu 130 9
Ty l§ (%) 6,9
cac vi khuan
Hps 6 9 15 11,5
gay benh ho hap tren heo (%) So vi khuan
Pm 6 6 12 9,2
Bb 3 3 6 4,6
Tong(%) 19(14,6%) 23 (17,7%) 42 (32,3%)
App: Actinobacillus pleuropneumoniae, Hps: Haemophilus parasuis; Pm: Pasteurella multocida;
Bb: Bordetella bronchiseptica
Nhin chung, ty le phan lap dugc cac vi khuan gay benh hd hap tren heo trong nghign cuu cua chiing tdi co khac biet so vdi cac nghign cuu tmdc do tai cac khu vuc khac.
Trong nghign ciiu nay, Haemophilus parasuis cd ty le phan lap cao nhat so vdi cac vi khuan khac, chiera 11,5% tren tdng sd mau phan lap. Ty le phan lap Haemophilus parasuis tir cae mau benh pham tren heo ciing co khac so vdi cac nghign ciiu khac. Nghien ciiu ciia Zhao va cdng sir (2010) cho thay ty Ie phan lap Haemophilus parasuis la 26,7%. Trong khi ket qua nghign cuu cua Hricinova va cdng su (2010) cho thay trong tdng sd 219 mau phdi, thi chi cd 4 raau duong tinh doi vdi Haemophilus parasuis, chigm 1,83%. Haemophilus parasuis gay viem da xoang bao gom vigra sgi ca, vigm khdp va vigra mang nao cd the thay d the cap tinh. Mang phdi, mang ngoai tim, raang bung, mang hoat dich va mang nao chira nhieu dich cd sgi fibrin. 6 thg man tinh, Haemophilus parasuis gay xa hda nghiem trgng mang ngoai tim, mang phdi va/hoac phiic mac, ciing nhu vigm khdp man tinh tren heo (Oliveira va Pijoan, 2004).
Pasteurella multocida la vi khuan cd ty Ie phan lap chigm 9,2%, diing thii hai sau Haemophilus parasuis trong nghien cim cua chiing tdi. Cu Huu Phii va cdng su (2005) cho rang nguygn nhan gay benh ho hSp trgn heo d cac tinh phia BSc chii yeu la Pasteurella multocida, Actinobacillus pleuropneumoniae va Streptococcus suis. Tuy nhien, cac cdng
bd d khu vuc phia Bac ciing tap tmng vao thic nghiera vacxin phdng benh do cac vi khuan tren (Hoang Van Minh va ctv, 2017; Cii Hihi Phil va ctv, 2013; Nguyen Thi Thu Hang va ctv, 2010). Chua cd nhigu cdng bd trong nude vg ty le phan lap vi khuan nay, ngoai tni nghign cuu ciia Dang Xuan Binh va cdng su (2010), cho thay ty le phan lap Pasteurella multocida tren heo tai Ha Giang va Cao Bang la khoang 5%. Cac nghign ciiu tir nude ngoai cho thay cd sir khac nhau ve ty le phan lap Pasteurella multocida. Ty le phan lap Pasteurella multocida trong mgt nghien cuu d Trung Quoc la khoang 8% vdi 233 vi khuan phan lap dugc tir 2912 ca benh (Tang va ctv, 2009). Nghign cim ciia Hricinova va cgng su (2010) cho thay ty le phan lap Pasteurella multocida tir mau benh pham trgn heo la kha cao, chiem 74,9%
tdng sd mlu phan lap. Pasteurella multocida gay benh tu huyet tren heo vdi cac bieu hien sdt cao, khd thd, xuat huyet d da vimg bung, tai, dui, mdng, ddi khi toan than. Pasteurella multocida toxin (PMT) serogroup D kgt hop vdi Bordetella bronchiseptica gay viem teo xoang mui tign trien. Benh lam bign mat hoan toan syn mui, mdm nit ngin va xoSn lai, chuygn mau sam va vigm phdi (Harper va ctv, 2006).
Ty Ie phan lap Bordetella bronchiseptica trong nghign cull nay la 4,6%, thap nhit so vdi cac VI khuan khac. Ket qua tir mot nghign ciiu khac cho th§y Bordetella bronchiseptica cd ty Ie phan lap kha cao trong cac mau benh phim tir heo, chigm 18,6% trong tong s6 mlu phan lap ' '^h kliacddng (Zhao va ctv, 2010). Cac rain
KHOA HQC KY THUAT THU Y TAP XXVI SO 6 - 2019
nhiem vdi Bordetella bronchiseptica lam tang mire do nghiem trong ciia benh. Bordetella bronchiseptica md dudng cho Pasteurella multocida gay vigra teo xoang mui tign trign.
Streptococcus suis, Haemophilus parasuis ciing c6 xu hudng ket hgp vdi Bordetella bronchiseptica gay vidm xoang miii, viem phdi lan rpng (Brockmeier, 2004).
Ket qua nghign ciiu ciia chiing tdi cho thay, vi khuan Actinobacillus pleuropneumoniae CO ty Ie phan lap la 6,9%. Tai cac khu virc khac, ty le phan lap vi khuan nay cao ban so vdi kgt qua nghign ciiu cua chung tdi. Ty le phan lap Actinobacillus pleuropneumoniae tren heo bi benh ho hap tai Bgn Tre va Can Tha dao dgng hi 20-24% (Phan Kim Thanh vactv, 2018; Nguyen Luang Trudng Giang va ctv, 2015). Mot nghien citu khac tir nude ngoai ciing cho thay ty le phan lap Actinobacillus pleuropneumoniae tu nhirng mau phdi heo thu thap tir 16 mo la 20,5% (Hricinova va cgng sir, 2010). Actinobacillus pleuropneumoniae la mgt trong so vi khuan quan trgng nhat gay viem phoi tren heo d mgi liia tudi va dugc tira thay rgng rai, Vi khuan gay tdn thuang phdi, xuat huygt bg mat va hoai tir, sgi fibrin bao phu bg mat phdi, ke ca ndi tang. Actinobacillus pleuropneumoniae cdn gay xuat huyet bao thai nghiem trgng. Ngoai ra, vi khuan Actinobacillus pleuropneumoniae cdn ket hop vdi cac tac nhan khac nhu Mycoplasma hyopneumoniae, cum heo hay PRRS lara trim trong cac tdn thuang tren dudng hd hap heo (van Dixhoorn va ctv, 2016; Pomorska-Mdl va ctv, 2017). Le Van Duong va cong su (2012), Nguygn Qudc Huy va cdng su (2013) nghign ciiu tir nhiing mau benh pham d heo duang tinh vdi virus PRRS tai B k Giang da cho kgt qua 19,59%
va 17,78% s6 mau dugc phan lap duang tinh vdl vi khuan Actinobacillus pleuropneumoniae (trich din bdi Phan Kim Thanh va ctv, 2018) Nhu vay, cac tac nhan gay benh lien he mat thiet vdi nhau, tao nen nhiing mat xich quan trgng, lam tram trpng cac van dg hd hap trgn heo, 0 thg man tinh, benh lam giam tang trong
trung binh hang ngay tren heo khoang 33,6%
va he sd bign chuygn thuc an hi 0,77 - 25,5%, lara heo cdi cgc, cham tang trudng, anh hudng dgn hieu qua kinh tg (Sassu, 2018).
Tir nhiing tdng kgt tren cho th§y, cd kha it cac cdng bd trong nude vg tinh hinh phan lap cac vi khuan trong nghign cuu nay, ngoai tni' ragt sd cdng bo da thao luan d trgn. Cac nghien cuu trudc day tap trung chii yeu d phia B^c va khu vuc Tay Nam Bg. Vi vay, kgt qua nghien ciiu nay la cong bd diu tien vg ty le phan lap 4 loai vi khuan gay benh hd hap trgn heo d khu vuc Ddng Nam Bg. Nhin chung, ty le phan lap cac vi khuan gay benh hd hap trgn heo la co khac nhau giii'a cac khu vuc khao sat, digu nay cd thg phu thudc vao digu kien ve sinh, quan ly an toan sinh hgc, viec sir dung thudc khang sinh trong phong va tri benh ciing nhu sir dung vacxin phdng benh cho heo. Ty le phan lap cac vi khuan gay benh hd hap trong nghign ciiu cua chimg tdi khac vdi cac nghign ciru khac va d miic thap, mac dii cac man thu thap de phan lap vi khuan dugc lay tren nhiing heo cd bigu hien benh ho hap. Cac trang trai va Id md da thu thap raau thudc khu vuc tinh Ddng Nai va Binh Duang. Day la nhiing khu vuc cd mat dd chan nudi heo ldn, nhat la Ddng Nai hien dugc xem la thu phii chan nudi heo cua ca nude, hien cd han 2,5 trieu con heo trgn toan khu vuc. Mat do chan nudi ddng, benh tat gia tang, ap luc sir dung thudc khang smh trong chan nuoi cao.
Ngoai ra, trong thdi gian thu thap mlu nghien cuu ciing la thdi digra xay ra dich Id mdm long mdng va dich ta heo chau Phi, digu nay cang lam tang ap luc su dung thudc khang smh tai cac trai de chdng phu nhiem vi khuan khac Do dd, lam cho vi khuan trd nen ygu di, giam kha nang phat trign ciia vi kliuSn trgn mdi trudng phan lap, tii do giam ty Ie phan lap. Ngoai ra, nguygn nhan gay benh hd hap trgn cac heo kliao sat cd thg con do tac nhan vi khuan khac nhu Streptococcus suis. Mycoplasma hoac virus gay benh roi loan hd hap va sinh san (PRRS) hay benh cdi coc tren heo sau cai sua do PCV2.
KHOA HOC KY THUAX THU Y TAP XXVI SO 6 - 2019
Hinh 1. Phoi heo bi hoai tCr do App.
Hinh 2. Khuan lac App. tren moi tru'cmg TSA sau 24gia'/37°C
Hinh 3. W khuan App, hinh cau triPc, Hinh 4. Ket qua PCR vi khuan App.
Gram am dwoi kinh hien vi do phong lane 1: ladder (ISOObp); 2: doi chimg duang; 3:
dai 1000X doi chung dm; 4-8: sdn phdm PCR gen apxfVA
IV. KET LUAN
Nghign ciiu cua chiing toi da gdp phan xac dinh 4 mam benh vi tning cd kha nang gay benhho hap trgn heo tai nhirng khu vuc da thu thap raau.
Trong dd, ty le phan lap vi khuan Haemophilus parasuis la cao nhat, tigp dgn la Pasteurella multocida, Actinobacillus pleuropneumoniae va Bordetella bronchiseptica. Ket qua ciia nghign ciiu nay la ca sd cho cac nghien cira figp theo vg xac dinh miic dp nhay cam vdi khang sinh hoac nghign ciiu benh hpc ciia nhiing vi khuan da phan lap dugc.
Lai cdm an: Trdn trong cam an Prof Qigai He, Phong Thi nghiem irong diem ve Vi sinh nong nghiep, Khoa Chdn nuoi Tint y, Dgi hoc Nong nghiep Huazhong. Trung Quoc da giup da chung loi ve quy ti-iuh phdn lap cdc vi khudn trong nghien ci'ni.
TAI LIEU THAM KHAO 1. BrockmeierS. L. (2004). Prior uifection with
Bordetella bronchiseptica increases nasal colonization by Haemophilus parasuis in swine.
Velerinaiy Microbiology: 99{\): 75-78.2.
2. Brogden S., Pavlovic A., Tegeler R.. Kaspar H., DeVaan N., Kehrenberg C.(2018).
Antimicrobial susceptibility of Haemophilus parasuis isolates from Germany by use of a proposed standard method for harmonized testing. Veterinaiy Microbiology 217: 32-35.
3. Cil Hiru Phii, Nguyen Xuan Huygn, Au Xuan Tuan, Le Thi Minh Hang, Luu Thi Hai Yen.
Van Thi Hudng, Tran Viet Dung Kien. Tang Thi Phucmg (2013). Xac dinh serotype vamgt so ygu t6 gay benh ciia \i khuan Actinobacillus pleuropneumoniae, Pa'-'..^ ' 'la multocida \a Streptococcus suis dc clion jvjna ch€ vaccin phong benh viem phoi L , Tap chi klioa
KHOA HOC KY THUAT THU Y TAP XXVI SO 6 - 2019
hoc 1^ thudt ihu y, ldp XX., so 7-2013.
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Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated frora diseased cattle and pigs across Europe: The Vetpath study Veterinary Microbiology 172(1- 2): 202-15.
5. Dang Xuan Binh, Nguygn Thi Kira Dung, Nguygn Thi Ha, Le Ba Hiep (2010). Khao sat su luu hanh cua Pasteurella multocida d ragt so gia siic mgt so tinh mign nui phia BJc Viet Nam. Tap chi khoa hoc ky thugt thii y, lap XVIl so 2-2010
6. Harper M., Boyce C. J, Adler B. (2006).
Pasteurella multocida pathogenesis: 125 years after Pasteur: Pasteurella multocida pathogenesis. FEMS Microbiology Letters 265(1): 1-10.
7. Hoang Van Mmh, Le Van Duong, Cu Hiiu Phii, TrSn Diic Hanh, Nguygn Quang Tuyen (2017).
Ket qua thir nghiem vaccin vo boat phdng viem phoi cho lgn do vi khuan Actinobacillus pleuropneumoniae, Pasteurella multocida va Streptococcus suis gay ra tai Bac Giang. Tgp chi khoa hoc ky thudt thu y, tdp XXIV, s6 5-2017.
8. Hricinova, M., Holoda E., Mudroiiova D., Ondrasovicova S. (2010). Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae. Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse. Folia Microbiologica 55(6):
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