Nghien
CLPU- Ky thuat
duct, give rise to the number of spermatic cords, sperms and improve the activities of Leydig cells
Tal lieu tham khao
1. Abrham W.B.: Techniques of Animal and Clinical Toxicology, Med. pub. Chicago, (1978) pp 55 - 68.
2. Bp Y te - Cong hoa Xa hoi Chii nghTa Viet Nam, Quy d|nh ve nghien ciru dupe ly cac thu8c y hoc co truyen dan tgc, quyit djnh 371/QB-BYT. (1996)
3. Bp Y te, Dy thao huPng dan thiT dpc tinh cua thuoc- Cac phu'ang phap thiT doc tinh cap - OECD,
Phil Ivc 2. (2005)
4. SO Y t i , Dy thao hudng d i n thu' dpc tinh cua thuoc-Cac phuang phap Tinh LD50 cd dien, Phu luc 3.
(2005)
5. Bp Y te, Du thao hypng d i n thO' dpc tinh cua thuoc-Phyong phap thir dpc tinh ligu nhac lai 28 ngay, dudng uong - OECD Guideline: Repeated Dose Toxicity 28 Days- Oral, Phu luc 3. (2005)
6. WHO: Research Guidelines for Evaluating the Safety and Efficacy of Hertjal Medicines, Manila, Philipin, p p 3 5 - 4 1 . (1993)
Khao sat tren thirc nghiem doc tinh ban trirong dien cua che pham mecook
Pham Viet Dy', Nguyen Thj Nhung^
Vien Y hgc co truyen qudn dgi Benh vien Da khoa tinh thucmg Nam Dinh Dat van de
C i y mach ba gdc l i vi thude tir l i u d i d u p e d i n gian dung de ehu'a e i e ehtrng b i n h b i o phi, x a vira ddng mach [1. 2] Thuoc mecook d u p e b i o c h i tir c i y mach ba gdc tai khoa D u p e V i i n YHCT q u i n dpi. Trong c i c n g h i i n eiru thue nghi$m t r u d c d i y chiing tdi d i khao s i t s a bp dpc tinh cap, k i t qua c h u a x i c dinh d u p e LD50. IVIue tieu eua n g h i i n ciru n i y l i x i c djnh dde tinh b i n t r u d n g d i i n eiia thude mecook qua mpt sd chi sd sinh hpe eua thd.
Ddi tu'p'ng va phu'ang phap nghien CU'U
Ddi lu'ang nghien c u u
- T h d thi nghiem: 30 con thd t r i n g chung New Zealand trpng l u p n g 2,2 ± 0,2kg, khoe manh, d ea 2 gidng v i d u p e nudi trong d i i u kien thi n g h i i m tai V i i n Kiem n g h i i m thude TLf- Bp Y te.
- Thude thu': Mecook dugc c h i d u d i dang v i i n nang h i m lupng 0,5g tir t o i n c i y mach ba gdc tai khoa D u p e Vi$n YHCT Q u i n dpi.
Phu'ang phap nghien cu>u T i i n h i n h theo t i i l i i u '^^"''
Thd d u p e chia l i m 3 Id, mdi Id 10 con:
- Ld chirng: udng dung djch NaCI 0,9%.
- Ld thii' 1: udng mecook lieu 0,12g/kg trpng l u p n g / n g i y .
- Ld t h u 2: udng mecook l i i u 1,2g/kg trpng l u p n g / n g i y .
Cdch tiin hdnh:
- Thude d u p e h o i tan v i o n u d e c i t v d i hai loai ndng dp 0,02mg/ml v i 0,2mg/ml
- The tich thuoc cho thd udng l i 5ml/kg v d i natri clorua 0,9% v i mecook d u p e pha v d i hai loai ndng dd t r i n v i o 9h h i n g n g i y , trong thdi gian 28 n g i y .
- C i c ehl t i i u d i n h g i i t r u d c va sau khi k i t thiic nghien ciru
+ Tinh trang chung, t h i trang, t h i n trpng eua thd.
+ C i e ehl sd h u y i t hpc.
+ C i e ehi sd sinh h o i : AST, ALT, creatinin, u r i .
+ Md thd, quan s i t dai the v i vi the td chirc gan, t h i n .
- C i e sd l i i u d u p e xir ly b i n g p h u a n g p h i p thdng k i y sinh hpe, cd so s i n h t r u d c sau v i so s i n h chirng - thir.
K i t qua nghien cu>u Tinh trang chung cua tho
Trong thdi gian thi n g h i i m , thd d c i e Id n g h i i n ciru d i u boat ddng binh t h u d n g , i n udng tdt, p h i n khd, Idng m u p t , khdng cd h i i n t u p n g rung Idng h o i c Idng khd cirng.
Anh htrd'ng cua thude mecook d i n trong lup'ng cua tho
TAP CHi DU'QC HQC - 7/2009 (SO 399 NAM 49) 25
•
Nghien
CLFU-
Nhdm
Bang 1
Trude TN
Ky thuat
: Sg thay dii trgng lugng thd trudc vd sau thi nghipm Cin ning (kg)
Sau Sau 1 tuan 2 tuin
Sau 3 tuan
Sau 4 tuan
P (trudc- sau) Chirng 2,07±0,15 2,17±0,14 2,24±0,12 2,29±0,14 2,34±0,12 < 0,05 Ld t h i r l 2,08±0,18 2,16±0,17 2,18+0,14 2,25±0,14 2,29±0,12 < 0,05 Ldthu'2 2,29±0,12 2,34±0,14 2,32±0,15 2,37+0,14 2,40±0,15 < 0,05 p (so vdi chirng) > 0,05 > 0,05 > 0,05 >0,05 Sau thi nghiim, trpng lupng thd tang lin rd
r i t vdi Ptru*c-sau<0,05 v i khdng cd su khic biit
giCra eie nhdm.
Anh hydrng din cac chi s6 xet nghiem mau Bang 2: Cdc chi si huyit hgc trudc vd sau thi nghiem
Ctiiso Hdng cau
xlO'^/l
Bacii c^u x 10'/l
P
P Huyet s i c td (g/l) P Hematocrit (%)
x i o ' / l P
P LdNC Ctiirng Ld thCr 1 Ld thir 2 Chu'ng Ld thCr 1 Ld thCr 2 Chirng Ld thCr 1 Ld thCr 2 Chu'ng Ld thCr 1 Ld thCr 2 Chirng Ld thCr 1 Ld thi> 2
n 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
K^t qua ngh Trifdc thi nghi$m
6,0 ± 0,34 6,3 ±0,25 5,8 ± 0,30 p > 0,05 7,5 ±0,15 8,4 ±1,02 7,5 ±0,57 p > 0,05 10,1 ±0,87 12,9 ±0,48 12,4 ±0,51 p > 0 , 0 5 39,9 ± 1 , 6 42,1 ± 1 , 5 39,5 ± 2 , 0 p > 0,05 336,0 ± 35,2 367,0 ± 52,3 297,7 ±40,5 p > 0 , 0 5
lien ciru Sau thi nghi$m
5,9 ±0,27 6,1 ±0,32 6,0 ±0,16 p > 0,05 8,1 ±0,34 9,2 ± 0,36 7,9 ±0,34 p > 0,05 12,3 ±0,46 12,5 ±0,51 12,5 ±0,38 p > 0,05 40,0 ± 1 , 4 40,4 ± 1 , 8 40,6 ± 1 , 3 p > 0,05 361 ± 40,2 411,3±35,5 422,4 ± 38,5
p > 0,05
P t r u & c - s a u
p > 0,05
p > 0,05
p > 0,05
p > 0,05
p > 0,05
Sau 28 ngiy udng thude, khdng thiy ed su thay ddi ed y nghTa thdng ke eie ehi sd huyet hpc eua thd, khdng cd su khic biit trudc sau nghiin ciru v i giCra Id chirng vdi Id tri.
Anh hudng cua vien nang mecook len chii>c nang gan tho
Bang 3: Cdc chi si AST va ALT
Sau 28 ngiy udng thude, ehi sd AST vp ALT a hai Id thCr v i Id chirng thay ddi khdng cd y nghTa so vdi trudc nghiin eiru (p>0,05).
Anh hudng cua thude mecook len chu'C nang than
Bang 4: Cdc chi si xdt nghiem chuc ndng thdn cua tho
Chi so LdNC n
Ket qua nghien ciFU
Trwo'c Sau Ptmoc.
thi nghiem thi nghiem Chu-ng 10 76,4 ±26,5 77,6 ±38,9 AST ("/I) L d t h i H 10 53,9 ±9,1 55,7 ±12,3 L6 thir 2 10 58,6 ±17,2 76,1 ± 19,6 P p > 0,05 p > 0,05 ChCrng 10
ALT ("/I) LdthCrl 10 Ldthi>2 10
115,9 ±37,5 125,6 ±29,8 92,7 ±17,4 102,5±22,3 101,1±18,3 110±11,4
p > 0,05
p > 0,05 p > 0,05 p > 0,05
Chi so
Ure (mmol/l)
Creatinin (nmoi/l)
L6NC
Chu'ng Ld thir 1 Ld thir 2 P
Chirng Ld thir 1 Ld thir 2 P
n 10 10 10
10 10 10
Ket qua nghien ciru TruvK
thi nghiem 2,40 ± 0,70 3,20 ±0,80 3,0 ± 0,70 p > 0,05 129.6 ±12,8 132.7 ±19,4 146,1 ±13,2 p > 0,05
Sau thi
, . . PiruOc-sau
nghlpm 2,30 ± 0,82 3,15 ±0,80
p > 0,05 2,9 ± 0,80
p > 0,05 110,7 ±15,8 103,9 ±13,9 120,1 ±21,8 p>0,05
26 TAP CHi D i r o C HQC - 7/2009 (SO 399 NAM 49)
Nghien
CLFU- Ky thuat
Sau 28 ngiy udng thuoc, him lupng uri v i creatinin trong miu tho d Id chung v i hai Id thir deu giam so vdi trude thi nghiim. Nhung khdng ed su khic biet ed y nghTa 2 nhdm thir v i nhdm chirng trude thi nghiim v i sau thi nghiim (p>0,05).
Nhirng thay ddi v i hinh thai mo hpc Hinh thai dai the gan va than thd sau udng thude 28 ngiy: Gan v i thin ed kich thude, miu sic, mat dd nhu md hoin toin binh thudng d ca 2 Id thu v i Id chirng.
Hinh thai vl the gan va than Bang 5: Hinh thdi vl thi gan va than
Lo nghiin cipu
Hinh anh vi the cua gan
Hinh anh vi the cua than Khdng sung huyet c^c tTnh mgch trung tam.
Ld chirng Khoang cira khdng cd x§m nhap viem. Cd thoai hda loan du'O'ng, thoSi hoa hat mirc dd nhe
Cau than binh thu'dng, khdng cd ton thu'ong d dng lugn gan, dng gdp va quai Henie
Ld thir 1
Hinh thai binh thudng d da sd thd. Hinh anh tho^i hda loan dudng, tho^i hoa hat, sung huyet tmh mach trung t§m va xoang tTnh mach 6' mirc 36 nhe
c l u true c l u than, dng lugn g i n , ong gdp, quai HeniS binh thudng. Bieu md dng than tru cao, b i t mau hdng dam, nhan ddu rd, trong dng than khdng cd tru hinh hole try hat. Rieng 1 than thd cd sung huyet nhe c l u than.
Gan c l u true binh thudng. Cac xoang tTnh mach gan cd bieu hien sung huyet nhe. Cac te bao gan g i n tTnh mach trung tam tieu thuy gan sang rd.
Khdng t h i y hien tugng (y ma hoac s i c td mat trong bao tugng t^ bao gan.
Ba sd cac c l u than sung huyet nhe, trong c l e bui mao mach c l u than ed chira mpt sd hdng c l u . Bieu md ong than rd. IVlgt so ong than cd tru Hyalin.
Khdng t h i y hien tugng thoai hoa hoac bong td bao dng than. Khdng t h i y tru hinh hoac try hat trong ong than
Ban luan
Sau 28 ngiy udng thude liin tuc vdi lieu 0,12g v i 1,2g/kg the trpng ehua thiy cd su thay ddi ehire ning sinh ly cua thd: thd d ca ba Id nghiin ciru vin an udng, sinh hoat binh thudng, dai tieu tiin binh thudng, khdng con nio bi rdi loan tiiu hoi. Trpng lupng thd ting theo thdi gian, ed y nghTa thdng k i so vdi trude nghiin cuu v i khdng cd su khie biet giira cie nhdm.
V i mdt sd chi sd huyit hpe, kit qua nghiin eiru niy cho thiy thude mecook khdng lim thay ddi ehl sd hdng ciu, bach cau, huyit sie td, hematocrit, tieu ciu. Dieu dd chirng td thude mecook ehua hoic khdng lim anh hudng xiu tdi chirc ning tao miu cua thd.
Cic xit nghiem hda sinh eua thd thi nghiem nhu AST, ALT khdng thay ddi sau thir thude. Kit qua xet nghiim md hpe cho thiy d gan ed tdn thuang thoii hda loan dudng va thoai hda hat mire dp nhe, nhung tdn thuang niy cung d i gip d mdt vii thd d Id chirng. Do dd, ed l i nhung thay ddi niy chua giy biin ddi v i sinh hda. Hinh anh binh thudng d cie md thin chirng td thude khdng lim thay ddi cau triic t i bio than eting nhu eic chi sd ehCre ning thanh thai ure, creatinin.
Kit luan
Qua kit qua thir dde tinh bin trudng diin 28 ngiy eua mecook trin 30 thd, chung tdi sa bd riit ra kit luin sau:
TAP CHi DlTQC HQC - 7/2009 (SO 399 NAM 49)
- udng thude mekook vdi liiu 120mg/kg v i liiu 1200mg/kg trpng lupng thd/ngiy, thd vin hoat ddng binh thudng, i n udng tdt, dai tieu tien binh thudng, Idng mupt. Khdng thd nio bi rdi loan tieu hoi. Thd tang ein deu d ea 3 Id nghiin eiru.
- Chua thiy su khie biit rd ret v i cic chi sd huyit hpc, hda sinh gan than giira nhdm chirng v i nhdm thir.
- Hinh anh dai t h i v i vi the eua gan, thin thd khdng ed su khic nhau nhieu giira nhdm chirng v i nhdm thir.
Summary
Fagopyrum esculentum Moench has been used to cure obesity, atherosclerosis, etc. in folk and traditional medicine. Recently, Mekook has been produced therefrom at the Pharmacy Department of Military Institute of Traditional Medicine, aiming to adjust the hypedipidemla syndrome. Sub-chronic toxicity tests showed that rabbits given Mekook at dose of 120mg/kg and 1200mg/kg per day remained normal In physical performane, eating, dnnking, stooling and urinating. Increase In body-weight was seen in all the three experimental groups. There were no noticeable differences in haematologlcal and biochemical indice, macro- and micro-images oi the liver No significant differences were noticed between the livers of the expenmental and control groups.
27
Nghien
CLFU- Ky thuat
Tal lieu tham khao
1. D 3 Huy Bich, Ding Quang Chung, Nguyin Thupng Dong v i cs. Cay thuoc v i dpng vat lim thuoc d Viet Nam, tap 2, NXB Khoa hgc va ky thudt. Ha Ngi, (2004) tr. 214-215.
2. Do Tat Lpi, Nhung ciy thuoc va vj thude Vipt
Nam (Xuit ban lin thir X), WXB Yhgc, Hd we/.(2003).
3. Hudng dan kim theo Quyet djnh so 371/BYT - QD ngay 12/3/1996 cua Bp Y t i ve xac djnh tinh an toan cua thude y hpc co truyen.
4. General guiderlines for methodologies on research and evaluation of traditional medicine. World Health Organization 2000.
Birfrc dau danh gia tac dung ha glucose huyet ciia re cay choc mau (Salacia cochinchinensis)
tren chuot nhat bi tang glucose huyet bang streptozocin
Do Thi Nguyft Que ', Nguyen Tran Thj Giang Huomg^
Nguyen Duy Thuan', Nguyen Viet Than' Nguyin Thi Kim Hue '
' Tne&ng dgi hgc Dugc Hd Ngi
^Truang dgi hgc Y Hd Ngi
^ Vien Duac lieu Dat van de
D i i thio dudng l i benh ed tde dd phat trien nhanh, dupe xem l i "dai djch" d c i c nude dang phit trien. Theo WHO, nam 2025, se ed 300 - 330 triiu ngudi mie benh, chiem ty l i 5,4% din sd toin ciu, trong dd chu y i u l i BTO type 2 (85- 95%) •''.
Viet Nam l i nude dang phit trien, benh DTD eQng dang ting lin nhanh ehdng theo thdi gian.
Dieu tra qude gia nim 2002 - 2003, ty le mie benh trong ca nude l i 2,7%, chu yeu t i p trung d lira tudi lao ddng 30 - 64 tudi (chiem ty l i 10,5%) v i chiim ty l i cao d vimg dd thi v i khu cdng nghiip (4,4%), die biit l i d c i c thinh phd Idn.
Hiin nay d i cd nhieu thude t i n dupe dupe su dung de diiu tri binh d i i thio dudng v i cho hiiu qua diiu tri tdt tuy nhiin hiu hit c i c thude niy ed nhiiu tie dung khdng mong mudn, die biit khi sir dung thdi gian d i i . Bin canh eie thude tin dupe, hien cung ed nhieu thude ddng dupe dupe nhin d i n sir dung de dieu tri d i i thio dudng '^'. Tie dung eua eie thude niy tuy khdng nhanh v i manh bing thude t i n dupe nhung thudng cd it tie dung khdng mong mudn, it dde tinh v i phii hpp vdi y i u cau dieu tri liu d i i ciia binh. Do dd, xu hudng nghiin ciru de tim ra
28
nhirng dupe lieu cd t i c dung ha dudng huyit vS diiu trj d i i thio dudng dang thu hut dupe nhieu su quan t i m cua eae n h i nghiin ciru.
Theo kinh nghiem d i n gian mdt sd nude, nhieu ciy trong loii Salacia nhu S. oblongga, S.
retlculat, S. chinesis, tir l i u da dupe dung de diiu trj bio phi v i d i i thio dudng cung nhu eic binh liu, khdp, hen....'^' Tai Nhit Ban, My vS mdt sd nude khic r i Salacia dugc diing dudi dang thuc pham chirc nang dinh cho ngudi tieu dudng hoic b i o phi. Nhieu nghiin ciru cung dS dupe tien hinh de x i c djn-h thinh phin hoi hpc, tie dung cua mdt sd c i y trong loii Salacia trong dd cd dinh g i i t i c dung ha glucose cua S.
chinesis '^•^•^' . Tuy nhiin, chua cd cdng trinh nghiin ciru nio ve t i c dtjng chua tieu dudng vA bio phi eua c i y chdc m i u - Salacia cochinchinensis - l i loii tim thiy d Viet Nam '".
Nham tim hieu tie dung g i y ha glucose huyet cua ciy chdc miu, chijng tdi tien hinh dinh giS tie dung giy ha glucose huyet ciJa loii niy trSn chupt nhit bj ting glucosse huyet bing streptozocin.
Nguyen lieu va phij'O'ng phap Nguyen lieu
R i c i y Salacia cochinchinensis Lour, dupe TAP CHi Dl/QC HQC - 7/2009 (SO 399 NAM 49)
