HOI NGHj KHOA HOC CONG NGHE SINH HQC TOAN QUOC 2013
K H A O SAT ANN HLfGNG CUA NUOI C A Y T H O A N G KHI LEN SLP SINH TRUING VA PHAT TRI^N CAY TRE MANH TONG (DENDROCALAMUS ASPER
"Betung Hitam")
Tran Trong Tuin', Huynh Le Thien Ti>, Nguyen Thi Kim Loan, Hguyin Thj Huyen Trang, D6 Ding Giap, Tha Xuan Du Vien Sinh hoc nhiet ddi, Vien Hin lam Khoa hoc va Cong nghe Vi$t Nam
T 6 M
TAT
Bai bio nay trinh bay mgt sfl kft qua ngbien cuu vi nhan gi6ng in vuro tir hat tre Mgnh Tong Dendrocatamus asper "Betung Hitam".
Sau khi duoc khir tnJng bing 50% javel trong khoang ttioi gian 20 phut, hat tre ducfc ciy len moi truong MS MS co bo sun§ B A (1,0;
3,0, 5,0; 7,0 mg/l) hoac kinetin (1,0; 3,0; 5,0; 7,0 m ^ ) v a nuoi u-ong dieu kien thoang khi de kich thich hinh thanh choi. Kel qua cho thiy mfll truong MS cd bfl sung 5,0 mg/l BA CO kha n3ng tao choi tot nhat (1,33 choi/hat). Tiep iheo, moi truong ra re MS c6 bo sung 3,0 mg/t IBA CO kha nang tao rl tfit nhat vdi be so 9 rc/chfli sau bon tuan nudi cay Ngoai ra, dieu kien nudi cay thoang khi cung giiJp cho ch6i sinh truang va phat tnen tfll bon. He sfl quang bop tlman cua tre Manb Tong dat dugrc cao nhSt la 0,65 fimol mot ' h"' cay"' crng^ythu21 trong dieu kien thoing khi.
Tli' klioa: Chat kich thich smh tnidng, Dendrocatamus asper, nuoi cay thoang khi, tre Manh Tdng, vi nhan giong GtOl THIfU
Vd'i nhi4u Igi ich thiet th\rc da du^c chO'ng minh, cay tre thirc sir la mot doi ti/q'ng nghien ciJu Uip din va da co nhieu cdng bo co giS tri ve m^t Khoa hgc cung nhu- thi/c tiin. Nam 1968, liln dau tign Alexander va Rao da tien hanh nghien ciru phdl tre va cho niiy mim thanh cay Irong 6ng nghiem. Tiep theo la nhu'ng nghiSn ci>u ve phan tap te bao trin (Tseng va cs., 1975). Nhom tac gia da thanh cdng trong viec tai smh c^y con Bambusa amndinacea tir phdi vo tinh (Mehta va cs , 1982) Cho din n§m 1989 da co hang loat cac cong trinh nghien CUTJ duoc tien hanh tren 15 loSi ttr 7 gi6ng tre khic nhau.
d Vi^t Nam vi cac nud'c trong Wiu vi,rc Oong Nam A, tre Manh T6ng {Dendrocalamus -asper 'Betung Hitam') ii mot trong nhi/ng cay trong chinh mang tai nhieu tcri ich thilt thi^c. Tre tnrcrng thanh la nguon nguyen lieu xanh thay the trong ITnh vi/c x^y dung, trang tri npi that, ngdnh gily va dgt. Tgi cac khu vi/c ddi trpc, vung ven dap thuy dien, ven biln, vung nut, Ire du'grc bong vd'i muc dich chlng x6i mon dit, ngan gio bien, d6ng thm cung cap nguyen lieu cho cac tang nghe thu cdng my nghe. Mang tre vCra la san pham dygc u'a chupng, vCra la mpt mat hang xult khau quan trgng.
Tre Manh Tdng dirge trdng theo phuwng phap truyen thong, vi dy nhu' si> dyng gdc ti> cay mf, chill canh ho§c giam hom. Nhirng phuwng phap buyin thdng tin nhilu cdng cham soc, van chuyen, gay anh hu^ng den cay me do phai dao goc. Hi§n nay, quy md cflng nhif dien tich bong tre tang cao, cac phuwng phap truyen thing ktidng the cung d p mpt lirgng Id'n cay gilng bong mpt thai gian ngan. Vi thi, cac nha khoa hpc da nghien cCru va i>ng dung phirang phip nudi cly md t l bio thyc vgt de nhan gidng tre Manh Tdng, trong dd phtrong phap vi nhan giong mo ra kha nang nhan nhanh, tao mdt so luwng Id'n ciy giong trong mgt tlioi gian ngin. Chi sau 3 thang, cSy gilng in v'ibv cd the chuyen ra dit trong voi ti tg sing sdt ten din 85%. Bai bao niy binh bay mgt so kit qua nghign ci>u buirc diu v l anh huwng cua dieu kidn nudi cay thoing khf len sy sinh tnwng v i phat tnen cua cay tre Manh Tdng in vitro.
VAT UEU VA PHUXXNG PHAP Vlt lieu
Hat be Manh T6ng dirge nhap khiu ti> Thai Lan.
PhiFgng phap
K/n> fnjng hat: Hat tre Manh Thdng dugc khu* trung bang dung djch javel 50% trong tho'i gian 20 phut.
Khio sat anh hirong ciJa su> thoang khi din kha nang tao choi cua hat tre li/lanh Tdng
Sau khi khi> trung, hgt tre diroc d y ten moi bucrng tao chli MS (Murashighe va Skoog, 1962) tnang hgp Magenta cd bo sung chit dieu hoa sinh tnrcrng BA (indote-3-butyric acid) hogc KIN (kinetin) vcri ndng do lln lirgt la 0,0; 1,0; 3,0; 5, 0;
7.0 mg/l. Moi nghiem thijc thyc hien 9 miu va lap lai 3 tin.
Chi beu theo doi- TTidi gian nay mim trung binh, so chli, chilu cao choi, s l ta/mlu, s6 la mcr, dien tich l i . Khao sat anh huvmg cua SIP thoang khi den sy tao re cda tre Manh Tong
Chll tre dugc ciy sang mdi tru'O'ng MS bo sung NAA (1-naphthaleneacetic add) ho|c IBA (lndole-3-butyric acid) vd'i ning dd tin lugt la 0,0,1.0; 3.0; 5. 0; 7,0 mg/l. Moi nghiem thijc thyc hien 9 mau val#p lai 3 tan.
Chi tigu theo doi. Chieu cao d y , chilu dii re. thd'l gian tgo rd bung binh, so rd/miu, s6 ta'mlu.
Khao sat anh hirong cua sy thoang khi den khi nang quang hgp thuin cua tre M?nh Tdng
Sau khi xic djnh dutyc thanh phan va nong dd thich hgp cua cac chit dieu hoa sinh biwng, chiing tdi tiln hinh khSo sit
khi nang quang hop thuin cua cay va so sinh vdi kha nang nay trong dilu kign nudi cay tfioang khi Choi dugc d y
vao cac hop Magenta chua 50 ml mdi tnrd'ng nudi d y ; 50% sd hOp dugc d$y kin (doi c*iung - Dl); 50% sd h0p c6n igi
HQi NGH! KHOA HOC C O N G NGHE SINH HOC TOAN QUOC 2013
f ^ s ' f ' ' ^ ^ " ^ ^ " * i fiai '5 va bit tgi b i n g mang miltipore 0.2 p m (Nghiem thu'c M l ) . Moi nghiem thuc t h y c hien 9 m a u va Igp
LTii Dcu tnoo doi: I )i(*u s u i t q u a n g h u p l l i u a n (pmol r n o l ' ^ T a y ' ' h'^) d u o c xac dinn Dang p h i r o n g p h a p o J a Fujiwara v a c o n g s y (1987), d' cac ngay nudi d y t h u 7 , 1 4 , 2 1 .
f ^ i f ° T ' ^ ' ' ' ' ^ ' P • ' ' * ' ' s o ' ' ! " • • So hdu d u o c xu- theo p h u a n g p h i p t h i n g ke ciia Duncan (1995) b i n g p h a n m g m S P S S Ib.U v a T-test
K ^ T Q U A V A T H A O LUAW
A n h hu-OTig c i i a S I P t h o a n g k h i d i n k h a n a n g t a o c h o i c u a h a t t r e M a n h T d n g
^f^y^ ^*^^° F^* ^?^ hud'ng cua BA v i KIN len s y t g o chdi cay b e Mgnh T d n g cho thay khdng c d s y khae bi^t d a n g k e ve t h g i gian nay m a m cung n h u so l u g n g choi d u a c hinh thanh (Bang 1) T h a i gian hinh thanh choi t h i i t h i l u trong (Slu ki^n thoang khi la b o n ngay (nghiem thirc c 6 bo s u n g 7,0 mg/l KIN) v i tdi d a l i 8,63 ngay (nghigm t h u c c d b o sung 1 mg/l BA).
Bang 1 . Anh hu'dng cua s y thoang khi den kha nang tao ch6l tre iManh Tong sau bfin tulin nu6i c l y Ctiat dieu hoa s i n h
trvcrng (mg/t)
1.0 3.0 7.0
T h M gian nay mam trung binh (ngay) 8,63a- 5.00b- 5,90b 5,00b 4,75b 4,37b 4,50b 4,00b
S o l u w n g choi trung binh/hat
1.22a 1.10a 1.33a 1.00a 1.00a 1.00a 1,00a 1,00a
'Cic chd ciiavab thd hi^n s y khic biit cd y nghia thdng kivdipS 0,05 trong phep thd Duncan's test
B6n cgnh d d . khi s o s i n h v o l k i t qua nghien ciJu ciia T r i n Trpng Tuan v i c o n g s y (2012) ve q u i trinh tgo c h l i tre Manh Tdng g d i l u kien khdng thoang k h i {sir d y n g nap d ^ y b i n g nilon) cho t h i y cd s y khae nhau v l tho'i gian nay m i m . Hgt trong dieu kign khdng t h o i n g khi d n it t h a i g i a n h g n d l n i y m i m (can 2.4 d i n 4.13 ngay d l nay m a m ) s o v o l thoang khi. Bdn c g n h d d . n l u n h u trong d i l u k i f n t h o a n g k h i , nghiem t h u c c d b l s u n g 5,0 mg/t BA trong mdi b u d ' n g dgt c h i tieu so l i r o n g c h l i 1,33 choi/hat thi d' d i l u ki?n khdng t h o a n g k h i , trong mdi tnro'ng b l s u n g 3 mg/l BA, c h i tieu so choi dat d i r g e d i n 6,53 c h l i / h g t (Tran Trpng T u a n v i c s . , 2012). K i t q u i n g h i i n CLPU nay cung t u g n g t u n h u nghidn c i i u cua v i Cha-um v i cong s y (2011) t r i n c i y M a c a d a m i a (Macadam/a tetraphylla L. 'Keaau') K i t qua cua nghien CLPU n i y cho t h i y b g n g l u g n g t u g i cua choi thu d u g c cao n h l t tren moi t r u o n g c d bo sung 2 mg/l BA v i dieu kien nudi d y t h o i n g k h i ( n i n g d p COz la 1000 j j m c l moi"'')
Bang 2. Anh huong cua s y t t i o i n g khi d§n cac chi tieu sinh tru'fliig vk phat trien ciJa choi tre Manh Tong sau bon tuan nuoi cay
trufl'ng t h y c NAA (mg/l)
1.0 3.0 5.0 7.0
v | t IBA (mg/l)
1.0 3,0 5.0 7,0
Chieu cao choi (cm) 1,23de
0,90e 2.29bcd 1,59cde 3,e3a 3,79a 2,73ab 3.52ab
So la hinh
1.00b 1.00b 1,00b 1,00b 1,13ab 1,50a 1,50a 1,20ab
0,0b O.Ob 0,0b 0,0b 0,2Sa 0,37a 0,0b 0,2a
Dien tich la
0,0b 0,0b 0,0b 0,0b 0,07a 0,87a O.Ob 0 203
'Cic chd ciiavab UiS hiin s y khic biit c6 y nghia d mde tin cay ps0.05 trong phep thd Duncan's test.
Tnang dieu kign t h o i n g k h i , c h l i p h i t t r i l n d i i h o n s o v o i dieu kien khdng thoang k h i , c h l i cd t h i dgt d u g c t u 3.79 - 3,83 cm b o n g mdi t n r i r n g lan l u g t b l sung 3,0 v a 1,0 mg/l KIN (Bang 2). Theo ti t h u y l t . n i n g dd C O ; c a o trong d i l u kien t h o i n g k h i se c6 Igi c h o s y p h i t t r i l n j a . Wottenng (1990) da c h u n g minh rang nong d p C O ; tren 10000 p p m rat c d fch cho c i y hoa H i n g v i c i y l i o a O i n g t i l n trong vigc g i a m s y lao hoa cua l i v i s y rgng la. Figueira v a Janick (1994) n h i n t h i y t i n h gia tang c i i a vigc keo d i i c h l i . s l l i , dign tich la, trgng l u g n g k h d v i t u o i ciia chdi c i y Theobroma cacao khi n i n g d d COz xung o u a n h ten d i n 2 4 0 0 0 ppm. Theo nghien c u u cua Cha-um v i c^ng s y (2011), k i t q u a cung cho t h i y v * i d i l u kt#n nudi cay thoang khi c d n i n g dp COz 1000 pmol m o r ' thi s l lidu v l c h i l u c a o choi va d i ^ n t i c h l i thu d i r g e cao h a n v d i d i l u kign nudi cay nong d g C 0 2 c h i cd 350 pmol meri"'.
A n h h i P ^ g ci>a s y t h o a n g k h i d e n s y t ^ o r i c i i a t r e M a n h T o n g
Khi k h i o s i t anh h u a n g cua NAA va IBA len s y hlnh r l c i i s c h a tre M g n h T d n g , k i t q u i t h i n g kg ( B a n g 3) c h o t h i y k h l n g c d s y k h i c bi$t ve ttidi gian t g o r l . Tuy nhien. c o s u k h i c bigt v l s l r g trung b i n h / m l u , so r e b u n g binh dgt cao n h l t d nghiem thurc c d b l s u n g 3,0 mg/l ISA (9,00 re/chdt). D l i c h i l u v * i k i t q u i tgo r l c u a Tuan v i c g n g s u (2012), c 6
H Q I N G H ! K H O A H O C C O N G N G H £ S i N H H O C T O A N Q U 6 C 2 0 1 3
the nhgn t h i y 6 d i l u kign t h o i n g k h i , cac nghiem t h u c nJt n g i n thd'l gian tao r l tCr 3 d i n 4 n g i y . D u a n g T u i n N h y l v a c o n g s y (2011) da c h i r n g m i n h r i n g trong d i l u k i e n nuoi d y thoang khi c a y c d k h a n i n g hinh t h a n h h g re t h i r d p n g a y t r o n g giai doan in vitro, dieu nay se d i thien ti le s i n g sdt cua cay khi d u a ra vud-n u a m .
Bang 3. Anh h u v n g cua s y thoang khi den t h M gian tao r l frung binh va so r l hinh thanh cCia tre Mgnh Tdng sau 4 tuan nuoi cay Chat dieu hoa sinh T h v i gian tao
tnroTig t h y c vat rS trung binh
NAA (mg/l) IBA (mg/l) (ngay)
7,0
14.00a 15,33a 13.00a 16.33a 15.00a 15.67a 16.33a 13.00a
4,33c 6.00b 2,33c 2.67c 3,67c 9.00a 4,67c 6,33b
*Cac chd cii avibthe hi$n s y khic biit cd y nghia thdng kivdipi 0,05 trong phip thd Duncan's lest
K i t qua s o s i n h s l re tgo t h i n h v i c h i l u d i i r l . cr c a hai d i l u kien t h o i n g kii^f v i k h l n g t h o i n g k h i d i u c h o t h i l y IBA cd k h a nang kfch ^ i c h t g o r l tot h o n N A A . K i t q u a n i y g i l n g v 6 i k i t q u a t g o rg b i n Dendrocalamus asper ( A r y a va c g n g s y , 1998) khi bo sung IBA vd'i n i n g d g 10,0 mg/l c h o ket qua tgo r e 9 6 . 0 3 % , v a i s o r l t n j n g b i n h 3 0 . 0 , c a o h o n 3 l i n s o v g i trud'ng h g p t ) l s u n g 3,0 mg/I N A A v i o mdi b i r o ' n g nuoi d y . T i c d u n g ciia IBA c u n g d t / g c n e u t r e n c i c n g h i e n c u u tao r l d l i vd'i cay Dendrocalamus longglspathus ( S a x e n a va Bhojwani, 1993). C d hai g i i t h i l t c h o u u I h l t a o rg trong d i l u kign thoang k h i . Trong d i l u kign t h o a n g k h i . c i c c h l i d u g c tang c u d ' n g k h i n i n g q u a n g h g p d o t r a o d o i khf tot h o n v o l khdng k h i , vl vgy s y sinh t n r d ' n g v i p h i t t r i l n cCia t h a n , r d va c a c bg p h g n k h a e t i t h o n . H o g c t h e o g i i t h i e t thCr hai, v d l d i l u kign t h o i n g khi. s y b i c h o i nude dien ra m a n h h a n , t u g n g nud'c trong m l i tnrd'ng g i i m . D o t i c d g n g n a y n d n h e t h i n g re d' chdi trong d i l u kign t h o i n g k h i p h i t b i l n v u o t trgi so v o i trong dieu kidn khdng t h o i n g k h i . T h e o n g h i e n CU'U cua Q u y n h va cgng s y (2002) tren c i y d phg, khi m l u d y d u g c nudi trong dieu kign thoang khi (2 m a n g mitipore), c h i l u dai re cao h o n so v d i nghiem thuc khong t h o i n g khi (24,7 m m so vd'i 10,5 m m ) . K i t q u i nghign ci>u ciia C h a - u m v a cgng s y (2011) cung cho t h i y c i c m l u nudi d y dud'i d i l u kien thoang khi ( n i n g dp COz la 1000 p m o l moi"') c o hg r l p h i t trien t i t h a n so vol d i l u ki$n k i m t h o i n g khi h g n .
A n h h u g n g cOa s y t h o i n g k h i d i n k h i n a n g q u a n g ho>p t h u a n c i i a t r e M g n h T d n g
Khi quan s i t c i c chf tieu sinh t r u o n g c u a cac c h l i d dieu kien nudi cay thoang k h i v i khdng t h o i n g khf d B a n g 4 , chung tdi t h i y c i c c h l i d u o c nudi d y trgn mdi b u d n g cd bo s u n g 3,0 mg/t IBA ir d i l u kign thoang k h i c 6 c h i l u d i i r l trung b i n h / m a u , c h i l u cao c h l i trung binh v i s l la d i l u c a o h a n trong d l l u kien nudi c l y k h d n g t h o i n g k h i .
B i n g 4. Anh hu'dng ciia s y thodng khi den cac chi tieu sinh b i r d n g cua tre Manh Tong trong q u i trlnh tao r l sau bon tuan nufli cay Dieu ki#n nufli cay C h i l u dai re(cm) Chieu cao choi (cm) So la (cai)
Khong thoSng khi 0.27 ± 0.02b 4,03b 3,03b TboSng khi 9,17 ± 1.48a 6,73a 4,33a 'Cic chd cai avibthi hi^n s y khic bl$t cd ^ nghia diing kdvdip^ 0.05 trong so sanh bing phuong phip T test
Higu s u i t q u a n g h g p thuan gi&a hai nghigm t h u c c d s y k h i c biet ro rgt. V d i n g h i § m t h i r c D C , higu s u i t q u a n g h g p t h u i n rat t h i p (< 0,2 pmol mot'^ h ' cay" ) v i g i i m d i n s a u 3 Uian nuoi d y . T r o n g khi d d , n g h i e m t h u c M l c d t i i g u s u i t q u a n g h g p t h u a n tang sau t h d i gian nuoi d y . d n g a y t h u 2 1 , hieu s u i t q u a n g h g p t h u a n c u a n g h i g m t h d c M l d a t g i i tri c a o n h l t (0.65 p m o l moi"' h'' c i y ' ) ( B i l u dd 1).
0.7
£ 0.6
0 5 - - 0 5 1 - - ii-^^
o 0.4 '
f ^ n o i ^ 0 ' 2 1 D C
• o < « 0 . 2 1 - - * ' - = ^ = . ^ . 0.13 O i l
E *J 0 1 ! -1"::::=—r.-.-^ -.- .r - e - M 1 0
8 10 12 14 16 18 20 22 24 Ngay
Hinh 1. B i l u d l anh h i r d n g ciia s y thoang khi den kha nang quang h y p t h u i n cila tre Manh T o n g '' D o i v d i n g h i g m t h d c D C , vi n i n g d $ COz tnang hop nudi c l y ludn t h a p d o h | p k i n ( s 6 t i n trao d & k h i v d i m d i b u - d n g ddi
v d i h g p M a g e n t a khdng cd l l b a o d l i khf chi la 0,2 t i n / g i d ) d a n d e n l u g n g CO2 d n thidt c h o q u i trlnh q u a n g h y p k h d n g d a y d l i . Q u a n sat c i c hgp Magenta cua n g h i e m t h u c D C c d s u dpng h o i n u d e , cho thay dfl i m turong d o i trang h | p c a o .
II13O
HOi NGH! KHOA HOC CONG NGHE SINH HOC TOAN QUCC 2013
lim cho tre Manh Tdng^thoit hoi nude chgm. Vi viy, khi nang hip thu nude va chit khoang eua tre ti> mdi trudng ttiap.
fl*""k!? - .'^°I?.9 " " ° ' "^y ^"dc d i y kin t i nguyen nhin cua sy tich tuy nhung hgp chit dugc phdng thfch. Bdi vi sy trao
• ggi Knl^^vgi m^iii tnrdng ben ngoat-han che non ndng dp CO2 liung liQp nufll d y giim din theo thdi gian. Cung vol sy giam no^ng do cOz t i sy gia lang khi ethyten Khi ethylen diroc bilt den nhu la mdt chat dilu hda sinh trudng thyc v^t.
Tuy nhien g nong dg eao, khi ethylen l i mpt chit giy dgc dli vdi thyc vat. Nong dp ethylen trong hpp nudi d y tang din theo thoi gian. din 2 ^M/t tir 21 din 60 ngiy sau khi d y (Dugng "Tan Nhgt, 2011) Ning dd ethylen thudng tang cao troiig eac hpp nudi ciy kem thdng thoing. Cic khi nay du-gc bilt t i co anh hudng din nhilu khia cgnh trong sy smh buong va phat trien cua thyc vat. Jackson v i cdng su (1991) da chung minh ring cd sy giim dd md cua la FIcus lyrata mgt each na ret v i su lun cua choi cung vdi sy cang phong, hudng ngang dit (diageotropism) v i sy thu nho cua la cay Khoai tay phg thuoc vao su tich tuy ethylen trong nhung binh dugc day kin. Khi tich tuy ethylen trong hop nudi d y trong mgt thgi gian dai, ethylen g nong dd cao trd thanh mot chat i>c chl ting frudng ciia thuc vgt v i thiic dly qui trinh lao hda (Buddendorf - Joosten va Wolterinq, 1994) Ning dd ethylen trong dng nghidm cd trao dli khi la 0,01 ml/1 va trong Ing nghigm day kin khdng cd sy trao ddi khi la 0.3 mt/l sau 20 ngay nudi cay Brassica campestris (Lenttni v i cgng sy, 1987). Kozai y i cdng su (2005) da dua ra ket luin cho rang vdi ning dp ethylen g nong dd cao se lam giam kha nang quang hgp eua thye vgt. Magdalita v i cgng sy (1997) cho rang ethylen tich tuy d frong mdi budng nudi d y dit than Carica papaya L. se lim ciy cham phat triln v i giy hien hugng ryng l i .
Nghi§m thi>e Ml cd higu suit quang hop thuan cao hon so vdi nghigm thuc DC v i tang sau thdi gian nudi d y do cd sy trap doi khi vdi mdi trudng (sl lan trao dli khf dli vdi hpp Magenta c6 hai l l trao do! khf la 3,96 tin/gid). Ciy phat biln tdt do khi nang quang hop dien ra mgnh hon so vdi nghiem thu DC, ddng thdi viic thoit hoi nude tit cung lim ro cd su tiep xue giu'a la v i cac b§ phgn khic cua ciy vdi khdng khi. Nguyin Thj Quynh v i Hoing Mlnh Tuin (2009) cung nhgn thay kH nuli cay ciy Hung chanh trong cic bao nylon ed gin ming trao doi khi vdi s l tan frao dli khf tuong dng 4,2 den 5,5 tan/gid, da giup ciy phat tnln t i l hon so vdi khi nuoi d y trong dieu ki§n khdng cd gan ming trao dli khi (so lln trao doi khf 0,7 lan/gid).
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Hlnh 2. Anh hu'dng ciia s y thoang k h i den s y sinh t r u w n g v a phat trien ciia tre Manh Tong. a l ; 1,0 mg/l NAA; a2: 3.0 mg/l NAA;
a3; 5,0 mg/I NAA; a4: 7,0mg.1NAA; b l ' 1,0 mg/l IBA; b2 3,0 mg/l IBA; b3-5,0 mg/l IBA; b4 7,0 mg/l IBA. c l , c2, Nghifm thuc thoang khf (c6 hai 16 trao d l i khi); d l , d2, Nghifm thuc doi chirng {kh6ng cd ho trao flfli khi)
KET LUAN
Trong null ely thoang khi, mdi trudng thich hgp cho khi ning tgo choi tir hgt tre Mgnh Tdng l i mli tnrdng MS cd b l sung 5,0 mg/l BA. Mdi trudng thich hgp oho vi§c tao r l cua tre Mgnh Tdng l i moi budng MS ed bo sung 3,0 mg/l IBA.
Kha ning quang hgp thuan eCia tre Mgnh Tdng trong dilu kign cd hai ming millipore trao dli khi vdi moi tnrdng sau 21 ngiy l i 0.65 pmol moi"' h"' cayV
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• Midm ldc gid xin chdn thdnh cdm on l^ign Sinh hpc Nliiet ddi da ho ircr kinh phi de thi_rc hien nghien cu-u nay.
TAl LI?U THAM KHAo
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EFFECT OF AERATION CULTURE ON GROWTH AND DEVELOPMENT OF DENDROCALAMUS ASPER "Betung Hitam"
T r a n Trong Tuan*, Huynh Le Thien T u , Nguyen Thi Kim L o a n , N g u y e n Thi Huyen T r a n g , D o Dang G i a p , Thai X u a n Du,
Institute of Tropical Biology
SUMMARY
The work presents llie establishment of a micropropagation procedure in vitro from Dendrocalamus asper "Betung Hitam" bamboo seeds under the aeration condition. After sterilized with 50% javel for 20 min, the bamboo seeds were cultured on 20 ml Murashige and Skoog's medium (MS) containing Ihe concentrations of BA: 1.0; 3.0; 5.0; 7.0 mg/l or Idnetin: I.O; 3.0; 5.0; 7.0 mg/l and grown in the aeration condition for shoot induction. The results showed that the MS medium containing 5.0 mg/l BA is suitable for the shoot induction (1.33 shoots per seed). Next, the MS medium containing 3,0 mg/l is Ihe best one for the root induction (nine roots/shoot after four week induction) The highest net photosynthetic rates were also observed after 21 day culture under the aeration condition.
Keywords Phylohormonc, aeration culture, Dendrocalamus asper "Betung Hitam", micropropagation 'Author for conrespondence, luan216lSiQmailcom