VIEN C H A N N U O I - Tgp chi Khoa hgc Cdng nghi Chdn nudi - S6 12- Thang 6 -2008
T I N H H I N H N H I E M GIUN S A N DUOfNG TIEU HOA d VIT CV SUPER - M NHAP NOI DUgfC NUOI THEO PHUONG THlTC B A N CONG NGHIEP
TAI TRAI VIT GIONG VIGOVA
Nguyin NggcHuan*
Trung tam Nghien ciru va chuyen giao tien bo ky thuat Chan nuoi 85/841 Nguyin V§n Nghi - P. 7- Q. Go VSp - Tp. Ho Chi Minh
*Tac gia lien he: Nguyen Ngoc Huan
Tel: (08)8.942,474 / 0913,710.423; Fax:(08) 8.958.864; Email:[email protected]
ABSTRACT
Study on helmintic infection of gastrointestinal track of CV SuperM ducks imported and kept under semi-industriai system
A study on was helmintic infection of gastrointestinal track of CV-super M ducks imported and kept under semi- industrial system conducted. The results showed that, three species of helminthes were found: Echinostoma miyagawa, Prosthogonimus cuneatus, and Microsomacanthus compressa. Total helminthic infection rate (%) was 31.2; infection rate {%) by E. miyagawa, P.cuneatus and M. compressa were 23.4; 9 and 7.5, respectively;
infection rate (%) of E.miyagawai in small intestine, large intestine, caeca and rectum were 1.2; 67.9; 88.9; and 61.7, respectively, A significant difference by helminthic infection was observed by age of duck, by season of year. An effect was shown in using Niclosamid to treat infection with M.compressa (100%) and with Trematod (59.11%) in ducks.
Keywords: Infection rate; infection intensity; E.miyagawai; M.compressa; helminth.
DAT V A N D E
Thiet hai do cac benh ky sinh triing gay ra d vit la dang ke, bao gdm vit cham lon, neu tdn thirc an cao, sire de khang giam ddi vdi benh tat nen anh hudng den chat lugng con gidng.
Trudc day, nhieu tac gia Nguyen Thi Le (1996a, 1996b); Biii Diic Lgi (1986); Luong Van Huin va Le Hiru Khuong (1996); Pham Him Phuoc (1998); Phan Nggc Anh (1999); Huynh Tan Phiic (1999) da nghien cim giun san ky sinh chu yeu d vit dia phuong, vit lai nudi theo phuang thiic tha ddng. Ndi dung cac nghien ciiu trudc day tap trung vao dieu tra thanh phan cac loai giun san ky sinh d vit, ty Ie va cudng do nhiem chu yeu tren vit thit nudi chay ddng d cac hd ndng dan, mdt sd bien phap tri benh. Tuy nhien, cac ket qua nghien curu d vjt CV- Super -M nudi nhdt trong dieu kien trang trai thi chua cd tac gia nao cdng bd. Mat khac, cdng tac gidng tai Trai vit gidng VIGOVA ciing dat ra yeu cau ve con gidng sach benh trong do cd cac benh ky sinh d vit. Tir cac van de tren, da tien hanh nghien ciiu de tai "Nghien ciru tinh hinh nhiem giun sdn duang tieu hoa a vit CV Super-M nhap noi nuoi bdn cong nghiep".
Muc dich nham: xac dinh tinh hinh nhiem giun san dudng tieu hda d vit dng ba CV Super - M nudi ban cdng nghiep qua cac chi tieu. Xac dinh thanh phan loai giun san; ty le nhiem; cudng do nhiem; thir nghiem tay giun san bang Niclosamid.
VAT LIEU vA PHU'ONG P H A P NGHIEN ClTU Vat lieu nghien ciru
Doi tugrng nghien cuu: Cac nghien ciiu dugc thuc hien tren 3 loai vit: vit con (tir so sinh den 2 thang tudi), vit hau bj (tir 2 fiiang tudi ddn 6 thang tudi), vit de (Uen 6 thang tuoi) da tien
NGUYEN NGOC HUAN - Tinh hinh nhiim giun sdn dudng tieu hda a vit...
hanh md kham 210 vit dng ba CV Super M dang dugc nudi tai Trai vit gidng VIGOVA.
TP. Hd Chi Minh.
Dja diim nghien ciru : Trai vit giong VIGOVA (so 94/1056, dudng Le Diic Thg, P. 17, quan Gd Vap, Tp Hd Chi Minh
Thffi gian nghiin cuu: tir thang 2 -7 nam 1999 va chia thanh 2 giao doan sau:
Miia khd tir 15/2-> 30/4 Miia mua tir 1/5-> 15/7 Noi dung nghien cuu
Xdc dinh thdnh phdn giun sdn ky sinh Xac dinh ty le nhiem giun san.
Xac dinh cudng do nhiem giun san.
Thir nghiem hieu qua tay giun san bang Niclosamid Phuong phap nghien ciiu
Cdc ky thudt sir dung trong nghiin cii'u bao gom: Ky thuat md kham khdng toan dien (chi kiem tra dudng tieu hda theo phuong phap ciia Vien sy Skrjabin); thu thap va ngam giir giun san trong dung dich Barbagallo; tinh danh giun san dua theo he thdng phan loai ciia NguyenThi Le (1996); trdn thudc vdi cam: trdn thudc theo lieu 0,6g thudc Niclosamidlkg the trgng vit, trdn mdt lan duy nhat.
C^c chi tieu theo doi Ty li nhiim
Ty Id nhiem (%) = Sd mau nhiem x 100 (3.1)
Sd mau kiem tra
Ty le nhilm theo lop (%) = Sd ca thd nhilm theo lap dd ^ J^Q ^3 2) Tdng sd ca the nhiem
— * Cuffng do nhiim
Cudng do nhilm la sd quan binh giun san tim thiy trong 1 ca the vit, dude bieu dien qua tri sd binh quan (mean) va sai sd chuan (SE)
Nhiim ghep cdc lodi giun sdn/cd thi
Ty le nhilm ghep chung (%) = Sd ca thd nhilm ghep ^ J^Q ^3 3) Tdng sd ca the nhiem
( Sd mau duong tinh trudc khi tay
- sd mau uuong tinh sau khi tay) ^ 100 (3 4) Hieu luc thudc tay (%) = Sd mau duong tinh trudc khi tiy
VIEN CHAN NUOI - Tgp chi Khoa hgc Cdng nghe Chdn nudi - S6 12- Thang 6 -2008
Xu ly so lieu
Sd lieu dugc xir ly bang phan mem SAS va Excel
Cac ky hieu viet tat trong bao cao: E_RN; E_RG; E_TT va E T S la E.miyagawai tuong iing d rudt non, rudt gia, true trang va tdng nhiem/vit.
KET QUA vA T H A O LUAN
Thanh phan va ty le nhiem cac loai giun san a duotig tieu hda vit CV Super -M
Ket qua cho thay d vit CVSuper-M cd 3 loai giun san la nostoma miyagawai; Prosthogonimus cuneatus; Microsomacanthus compressa.
Ty li nhiim chung (%)
Bang 1 Ty le nhiem cac loai giun san d vit nudi ban cdng nghiep Loai ky sinh
Lop Trematoda
Echinostoma miyagawai Prosthogonimus cuneatus Lap Cestoda
Hg: Hymenolepidae
Microsomacanthus compressa Chung toan dan
Sd md kham
(vit) 210 210 210 210 210 210 210
Sd nhiem
(vit)) 41 34 11 13 13 13 54
Tyle nhiem
(%)
19,52 16,19 5,24 6,19 6,19 6,19 25,71
Sd loai trong 1 Idfp(loai)
2
1 3
Ty le so vdi tdng sd loai
(%)
66,67
33,33 100 Ket qua Bang 1 cho thay, ty le nhiem chung la 25,71%, ty le nay thap hon so vdi dieu tra d vit nudi tai Thii Dire, Tp Hd Chi Minh la 56,3% (Biii Diic Lgi, 1986), 74,4%) d vit nudi tai Cin Tho (Pham Hun Phuoc, 1998), 61,6% vit nudi chay ddng tai huyen Binh Chanh, Tp Hd Chi Minh nhiem ky sinh triing dudng tieu hda (Huynh Tan Phiic, 1999).
Nhiim theo lop
Ky sinh d vit cao nhat thudc ve lop Trematoda chiem 19,52%, lop Cestoda 6,19%o (Bang 1).
Ty Id nhiem cac loai giun san thap so vdi cac nghien ciiu d vit chay ddng: Trematoda: 31,7%;
Cestoda: 18,3%; Nematoda: 30% (Huynh Tin Phiic, 1999); Trematoda: 50%; Cestoda: 100%;
Nematoda: 100% (Biii Dire Lgi, 1986); Trematoda: 60,02%; Cestoda: 61,01%; Nematoda:
40,9% (Pham Hun Phuoc, 1998).
Nhiim ghep giun sdn
Bang 2 Tinh hinh nhiem ghep cac loai giun san df vit
Loai nhiem ghep Sd vit md kham(con) Sd vit cd nhiem ghep (con) Ty le (%)
E.miyagawai+P.cuneatus 210 . 8 3,81 E.miyagawai+M.compressa 210 1 0,47 Tdng cdng 210 9 4,29 Ket qua Bang 2 cho thay, ty le nhiem ghep cac loai giun san ky sinh o vit nudi theo phuang
thirc ban cdng nghiep la thip (4,29%). Kdt qua nay thip hon so vdi cua Pham Hiiu Phuoc
NGUYEN NGQC H U A N - Ttnh hinh nhiim giun sdn duang tiiu hda a vit...
(1998) md kham vit tai huyen Thdt Ndt, tinh Cin Tho, cho ring ty le nhilm ghep 1-3 loai/ca the la 63,2%, nhilm ghep 4-6 loai la 25,6% va nhilm ghep tren 6 loai la 11,2%.
Thanh phan loai va ty le nhiem theo loai Echinostoma miyagawai
Ty Id nhiem Echinostoma miyagawai d dudng tieu hda la 16,19%. Ty le nay thip hon so vdi ket qua theo ddi d vit nudi chay ddng (30,05%) cua Pham Hiiu Phuoc (1998); va vit nudi tai Thu Dire, Tp Ho Chi Minh (30%) (Biii Dire Lgi, 1986).
Vj tri ky sinh ff duffng tieu hda : Ket qua trinh bay Bang 3
Bang 3 Phan bd vi tri ky sinh Echinostoma miyagawai d dudng tieu hda vjt (n=34 vit)
Vjt nhiem E.miyagawai S6lugng(vit) Ty le (%) 34 100
Ruot non (n=l) Ty le (%) 2,94
Vi tri Ruot gia (n=24) Ty le (%) 70,59
ky sinh Manh trang
(n=30) Ty le (%) 88,23
True trang (n=14) Ty le (%) 41,18 Microsomacanthus compressa
Ky sinh mdt phan d rudt non, chu yeu d rudt gia vdi ty le 6,19% nhilm Microsomacanthus compressa (Bang 3), thap hon so vdi ciia Hd Thi Thuan (1986) la 15%, nhung cao hon Pham Hihi Phuoc (1998) bao cao (3,8%).
Tinh hinh nhiim giun sdn d cdc lodi vit
Bang 4 Tinh hinh nhiem giun san theo loai vit
E RT E RG E_TT E RN E TS Pr BT Pr F Pr-TS SL TS S D M i c Tong GS
N 51 51 51 51 51 51 51 51 51 51
n 2
2
2 6 8
Vit cor
% 3,92
3,92 : 3,92 11,76 15,69
1
mean 1,50
1,5
1,5 1,33 (A) 1,37 (A)
SE 0,47
0.5
f
0,5 0,21 0,18
N 65 65 65 65 65 65 65 65 65 65
Vit hau bi n
2 3 1
3
3 7 9
% mean 3,08 1,00 4,61 2,00
1,54 1 (A)
1 4,61 3,0
4,61 3,00 10,77 2.0
(a) 13,85 2,55
(B) SE
0 0 0 0 0
0 0,22 0,38
N 94 94 94 94 94 94 94 94 94 94 94
n 25 21 13 1 32 7 6 11 35
35 Vitde
% mean 26,60 1,76 22,34 2,48
13,83 3,38 (A) 1,00 0 34,04 4,37
7,45 1,14 6,38 1,33 11,70 1,45 37,23 4,43
37,23 4,43
P SE
0,83 0,42 0,29 0,47
0,45 0.21 0,14 0,21 0,21 0,46 0.24
0,46 0,002 (*) P la xdc sudt linh ; (**) Nhiing gid tri cua sd binh qudn cd ciing cha giong nhau thi khdng khde nhau ngt cdch cd y nghTa a mure xdc sudt P<0.05.
VIEN C H A N N U O I - Tgp chi Khoa hgc Cdng nghi Chdn nudi - S6 12- Thang 6 -2008
Trong dd: N la sd mau vit kiem tra; n la sd mau cd nhiem ky sinh triing; Mean la sd trung binh mau; % la ty Id nhilm/mau kiem tra (%>); SE la sai sd chuan cua sd trung binh.
Ket qua Bang 4 cho thay, ty Id va cudng do nhiem nhu sau: Ty li nhiem sdn E-miyagawai:
Tdng so d cac loai vit la khac nhau (P<0,05): Thip nhit d vit con (3,92%) tang d vit hau bi (4,61%) va cao nhit d vit de (34,04%)). P.cuneatus chi gap a vit de vdi ty Id 11,70%).
Ty le nhiim M.compressa : la tuong duong (P>0,05) giira d vit con (11,76%)) va d vit hau bi (10,77%). Cudng do nhiim M.compressa: Giiiavit con (1,33 ± 0,21 san/vit) va vit hau bi (2,0 ± 0,22 san/vit) la tuong duong (P>0,05).
Tinh hinh nhiem giun san theo mua
Bang 5 Tinh hinh nhidm giun san theo miia
E R T E R G E T T E R N E T S Pr-BT Pr_Fab P r T S SL_TS SDMic SD_Hym
N 117 117 117 117 117 117 117 117 117 117 117
n 29 23 22 36 5 8 9 37 19
Miia khd
% 15,4 12,2 11,7 19,1 2,6 4,2 4,8 19,7 10,1
mean 1,6 (a) 2,2 (a)
1,4 (a) 3,6 (b)
1,00 1,5 1,9 3,9 (b)
1,9
SE 0,1 0,2 0,2 0,3 0 0,2 0,3 0,3 0,2
N 93 93 93 93 93 93 93 93 93 93 93
n 43 32 28 1 45 13 12 21 48 6 3
Miia mua
% 29,6 22,1 19,3 0,7
31 8,9 8,3 14,5 33,1 4,1 2,1
mean 1,8 (a) 2,5 (a) 1,7 (a)
1 4,6 (a)
1,5 1,1 1,6 5(a)
1,7 11,3
SE 0,1 0,2 0,2 ) 0,3 0,3 0,1 0,2 0,4 0,2 2
P 0,001
0,01 0,05 0,01 0,01 0,1 0.001 0,003 0,04 C*) P Id xdc sudt tinh ; (**) Nhirng gid tri ciia sd binh qudn cd ciing chir gidng nhau thi khdng khde nhau not cdch cd y nghTa d muc xdc sudt P<0,05.
Trong dd: N la sd mau vit kiem tra; n la sd mau cd nhiem ky sinh triing; Mean la sd trung binh mau; % la ty le nhiem/mau kiem tra (%); SE la sai sd chuan cua sd trung binh.
Ty li nhiim
Miia mua vit nhiem E.miyagawai : o rudt tit (29,6%), E.miyagawai a rudt gia (22,1%), nhiem P.cuneatus (8,9%), ciing nhu nhiem E.miyagawai tdng sd (31,04%) va san la tdng sd (33,1%)) deu cao hon (P<0,05) so vdi vit nudi miia khd (tuong iing la 5,1; 15,4; 12,23; 2,7; 19,1 va 19,1%). Vit nudi vao miia khd cd ty le nhiem M.compressa (10,1%)) cao hon (P<0,05) vit nudi vao miia mua (4,1%).
Cu&ng dp nhiim giun sdn theo miia
Cudng do nhiem E.miyagawai tdng sd : d vit nudi vao miia mua (4,5±0,35 E.miyagawai tdng sd/vit) cao hon so vdi d vit nudi vao miia khd (3,6±0,3 E.miyagawai tdng sd/vit).
So sanh cudng do nhiem san la tdng cdng: (bao gdm E.miyagawai, P.cuneatus) thi cd su khac biet mdt each dang ke giiia vit nudi d 2 mua. Miia mua ty le nhiem M.compressa mac dii thap
NGUYEN NGQC HUAN - Tmh hinh nhiem giun sdn duang liiu hda a vit...
(2,07%), cudng do nhiem thi kha cao: cudng do nhilm M.compressai la 11,3±2 san/vjt ma khdng thay d vit nudi vao miia khd.
Ket qua thu nghiem hieu qua thuoc tay san Nicosamid Hiiu qud tay sdn day vjt cda Nicosamid
Kiem tra hieu juc thudc tay Nniclosamid bing kidm tra tning san day qua mau phan.
Qua kiem tra mau phan trudc va 10 ngay sau khi tiy san day, kdt qua trinh bay d Bang 6 Bang 6. Ket qiia tay san la ciia Niclosamid
Trudc khi tay Sau khi tay
N n % Mean SE N n % Mean SE P'*' 30 6 20 5600(a)'' 1222,02 30 1 3,3 1600(a)'"' 632,02 0,001 ~
(*) P Chitest ()^): (**) Nhirng tri sd binh qudn cd ciing I chic gidng nhau thi khdng k/idc nhau mot cdch cdy nghTa a mice sdc xudt P<0,05.
Trong dd: N la sd mau vit kiem tra; n la sd mau cd nhilm ky sinh triing; Mean la sd trung binh mau; % la ty le nhilm/mlu kidm tra (%); SE la sai sd chuan ciia sd trung binh.
Ket qua bang 6 cho thiy, trudc khi tiy san day, ty le nhiem tning san day la 20%, vdi cudng dp nhilm la 23200,00 ± 12258,54 (tning/g phan).
Sau khi tay, khdng thay miu phan nao cd tning san day. Hieu qua tiy san la 100%. Dieu dd cd nghTa la hieu qua tay san day cua Niclosamid rat tdt.
Hiiu qud tay sdn Id & vit cua Nicosamid
Kiem tra hieu luc cua Niclosamid hkng phuong phap md kham vit vao 2 thdi dilm trudc va sau khi tay san bang Niclosamid, ket qua dugc trinh bay trong Bang 10.
Ket qua bang 7 cho thay, trudc khi tay san la, ty le nhilm E.miyagawai ky sinh d manh trang la 33,3%; d rudt gia 22,2%; d tnre trang 27,7%, tinh chung ty le nhilm la 38,9%.
Ty le nhiem san la rudt E.miyagawai a vjt trudc khi tay (38,88%) va sau khi tiy (12,5%).
Bang 7. So sanh nhiem ky sinh vat d vjt tnioc va sau khi tay bang Niclosamid.
E_TT
E T s o
Pr_Tso
S L T s o
Chi so n
% Mean SE n
% Mean SE n
% Mean SE n
% Mean SE
Trudc khi t a y ' " ' 5
27,77 3,40 0,24 7 38,88
5,29 (a) V y ; 0,78
2 11,11
1 (a) ^ e ; - . ' ' ^ - ! 8
44,44 4,88 (a) 0,93
Sau khi 0 0
2 12,50 1,50 (b) 0,50 1 6,25
1,00 (a) 3
18,75 1.33 (a) 0,33
tay 1 pC)
0,02
0,08
0,61
0,11
(*) a ddy Id gid trj P cua trdc nghiem Chitest (^); (**) Nhimg gid tri ciia sd binh qudn cd cimg chir gidng nhau thi khdng khde nhau ngt cdch cdy nghTa a muc xdc sudt P<0.05.
VIEN CHAN NUOI - Tgp chi Khoa hgc Cdng nghi Chdn nudi - S6 12- Thang 6 -2008
Trong dd: N la sd miu vit kidm tra ; n : sd miu ed nhilm ky sinh trimg; Mean: sd trung binh mau; % la ty le nhiem/mau kiem tra (%); SE: sai sd chuan cua sd tnmg binh.
Sir sai khac la cd y nghia d (P<0.1). Hieu qua cua tiy E.miyagawai ciia Niclosamid la 67,85%, dat hieu qua chua cao. Hieu qua tay san la tdng so la 59,11%, dat hieu qua chua cao.
KET LUAN VA DE NGHI Ket luan
Qua ket qua md kham 210vit, chiing tdi so bd cd mdt sd ket luan nhu sau:
Cdc lodi giun sdn ky sinh a vit nhu sau: Ldp Trematoda gdm 2 loai la Echinostoma
miyagawai, Ishii, 1932 va Prosthogonimus cuneatus (Rud, 1809) va Idp Cestoda cd 1 loai la Microsomacanthus compressa.
Ty le nhiim giun sdn chung : d vit la 31,2%. Trong dd, ty le nhiem Idp giun san Trematoda chiem ty Id cao nhat la 25,2% va Idp Cestoda nhiem la 8,4%.
Ty le nhiem giun sdn theo lodi: E.miyagawai ty le nhiem la 24,3%; P.cuneatus la 9% va M.compressa la 7,5%. Ty le vit bi nhiem ghep la 8,4% va nhiem ghep 2 loai. Ty le nhiem cua E.miyagawai d cac bd phan cua dudng tieu hda d vit la 1,2%) ky sinh d rudt non; 61,9% a rudt gia; 88,9% d manh trang; 61,7% d true trang.
Bien dong nhiem giun sdn theo logi vit: Cac loai vit khac nhau thi cd the nhiem nhiing loai giun san khac nhau: Ldp san day cd 1 loai M.compressa chi ky sinh d vit con va vit hau bi.
Nhiem E.miyagawai: cd khuynh hudng tang dan theo loai vit tir vit con -^ vit hau bi -^ vit de.
Nhiem san day cd khuynh hudng giam dan tir d vit con den d vit hau bi.
Bien dong nhiem giun sdn theo miia: Mua mua lam tang ty le va cudng do nhiem san la rudt E.miyagawai; trong khi vao miia khd, vit nhiem san day M.compressa cao ban mua mua. Thu nghiem hieu qua tay giun san bang Niclosamid: Hieu qua tay san la d vit bang Niclosamid chua cao va chi dat 59,11%) sau mdt lan tay. Hieu qua ciia Niclosamid ddi vdi san day d vit CV- Super M la cao va dac hieu, dat 100% sau mdt Ian tay.
Tdn tgi: Chua nghien ciiu dugc anh hudng ciia mdt sd yeu td khac nhu viec nudi tha ao, hd, vide bd sung mdi tuoi, rau xanh, anh hudng ao tha vit cd/khdng cd nudi ket hgp vit - ca va nhirng yeu td khac den tinh hinh nhiem ky sinh triing.
De nghi
Sir dung Niclosamid de tay giun san, nhat la san day cho vit CV Super M. Lieu tay la 0,6 g/kg the trgng. Nghien ciru tim thudc tay mdi, dac hieu de gidi thieu cho cac co sd nudi vit, nhat la cho he thdng nudi vit cao san CV Super M. Cho phep tien hanh thi nghiem tren quy md rgng va sd lugng mau Idn hon d mdt sd ddi tugng: a) vit gidng bd me, b) vjt gidng sieu trimg, nham hoan thien quy trinh phdng benh cho he thdng vit gidng cua Vigova va cac trai gidng cap 2.
NGUYEN NGQC H U A N - Tinh hinh nhiim giun sdn dudng tiiu hda a vit...
TAI LIEU THAM KHAO
Bill Diic Ltjri (1986). Dieu tra tinh hinh nhiem giun sdn va thii nghiem thuoc phong trir benh giun san tren \it nuoi tai huyen Thu Diic, Tp. H6 Chi Minh. Luan van tot nghiep Dai hoc, Truang DH Nong lam. Tp. H6 Chi Minh, tr.56 .
Huynh Tan Phiic, (1999). Digu tra tinh hinh nhilm giun san a vit tai huyen Binh Chanh, Tp H6 Chi Minh.
Luan van Thac sy nong nghiep. Truang DH Nong Lam. Tp H6 Chi Minh, tr. 58
Luang van Huan va Le HQTJ Khuang, (1996). Ky sinh va benh ky sinh a gia sue, gia cam. Tii sach Truang DH nong Lam Tp. H6 Chi Minh. T.l, Tr. 230
Nguyen Thj Le, (1996a). Giun san ky sinh a gia sue Viet Nam. Vien Sinh thai va Tai nguyen sinh vat. NXB Khoa hpc va Ky thuat, Ha Noi, Tr. 295
Nguyen Thj Le, (1996b). Giun san ky sinh a gia cam Viet Nam. Vien Sinh thai va Tai nguyen sinh vat. NXB Khoa hoc va Ky thuat. Ha Npi.
Pham Huu Phudc, (1998). Dieu tra tinh hinh nhilm giun sdn a vit tai huyen Thot Not, Can Tha va thir nghiem tay trir benh buou co vjt bang Tetramisole. Luan van Thac sy nong nghiep. Truang DH Can Tha, tr.76 Phan Ngpc Anh, (1999). Dieu tra tinh hinh nhiem benh giun chi Avioserpens taiwana gay ra tren vit tai Can Tha.
Luan dn Thac sy Khoa hpc Nong nghiep. Truing DH Nong Lam, Tp. Ho Chi Minh, Tr. 35.
*Ngu-di phin bifn: TS. Nguyen Thj Nga; TS. Bach Manh Dieu