Vietnam Journal of Physiology 19(1), 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 A N H H U ' O ' N G C O A C A O C H I ^ T L A C A Y L A DLFA {PANDANUS AMARYLLIFOLIUS)
BTN H O A T D P N G C U A E N Z Y M E G L U C O S E - 6 - P H O S P H A T A S E
& CHUpT BENH D A I T H A O D U ^ N G
Dai Thj Xuan Trang^" Nguyin Thj Al Lan^
T 6 M T A T ; Benh dii thio dudng (DTD) li benh do rdi loan chuyin hda carbohydrate vdi dac trung la tSng glucose huyit man tinh. Tinh trqng ting glucose huyet man tinh chiu chi phdi eua nhieu nguyen nhan khac nhau. Trong dd enzyme glucose-6- phosphatase (G6Pase) cd ehu ydu a gan va thgn ddng vai trd quan trgng trong vi$c glQ- cin bSng glucose trong co the. Muc tieu chinh cua nghiin eCru nay li danh gii khi nang hogt dgng cua enzyme G6Pase cd trong gan vi thin cua chudt benh DTD duqc dieu tri bing eao chiit ethanol ti ciy li diea (Pandanus amaryllifolius). Chudt duac giy benh DTD bing cich tiim alloxan monohydrate (AM) vao khoang bqng; ndng do AM duqc s d dung li 130 mg/kg trgng Iwqng. Sau 7 ngay u bgnh, ehuqt binh DTD duqc udng eao chiet li eiy li diea (400 mg/kg trgng luang/ lin x 2 lan/ ngiy) hoac thude dieu tri binh DTD thuang mai glucofast (150 mg/kg trqng luang/ lin x 2 lin/ ngay). Sau 20 ngiy diiu tri, gan va than cua ehugt trong cic nhdm nghien ciru duac giai phiu vi khio sit khi nSng uc chd hoat dgng cua enzyme GOPase. Kdt qui cho thiy eao chiet li eiy li dwa cd tic dqng ha glucose huyet d chugt binh DTD sau 20 ngiy diiu tri, glucose huydt eua nhdm chugt duqc diiu trj bSng eao chiit la ciy la dira giam khic biet cd y nghia thdng ki tu" 305,75 mg/dl xudng cdn 125,5 mg/dl va trd vi glucose huyet binh thuang. Hiiu qua Oc che hoat dong enzyme GSPase d gan va thgn chudt benh DTD dwqc diiu tri bSng cao ethanol li ciy la dira rit cao. Kit qua cho thiy ehuqt benh DTD khdng diiu tn cd luang phosphate vd ca dwqc tao ra a gan va thin lin luat li 8.3257 mg/l vi 3,0856 mg/l cao khic biet cd y nghTa thdng ke so vdi nhom chudt binh thuang 3,5902 mg/l vi 1,4258 mg/l Sau khi duqc didu trj bang eao ethanol la cay la dira; luang phosphate vd ca dwqc tao ra a gan va thgn cua chudt dwac dieu tri bSng eao la ciy la dira lan luat li 2,4914 mg/l vi 0.4119 mg/l. Ket qui thi nghiim chung minh ring, cao chidt la cay li dira ed khi nang hg glucose huyit trin chugt bgnh DTD theo ca chS ire ehe hoat ddng cua enzyme G6Pase.
Tip khda: Binh dii thao duang, ciy la dira. cao ethanol thi/c vat, Pandanus amaryllifolius. glucose-6-phosphate, enzyme glucose-6-phosphatase.
1. DAT V A N Dt 336 tneu ngudi [3]. Chinh vi vgy, Td Chuc Bgnh dai thao dudng (DTD) la benh Y Td Thd Gidi (WHO) dang bao dgng ve phd bien hien nay Tren thd gidi, benh can banh nay. Ban c h i t cua bgnh DTD la DTD gay t u vong cao thu ba sau b$nh tim su trao ddi glucose khong binh thudng.
mgch va bgnh ung thu. Dy doan den nam Sy thieu hoac sy khang insulin la nguyen 2030, sd ngudi mIe b^nh se tang den nhan d i n den s y rdi logn 16 trinh bien dudng carbohydrate. Cac enzyme lien ' Khoa hoc hgc Ty nhien, Trudng Dai hpc Can quan true t i i p d i n sy roi logn biln dudng J*^ . nay gdm hexokinase (EC 2.7 1 11
Tac gia chiu trach nhiem chinh: , ^ L U . J U J __
Dai Thj Xuan Trang ' glueose-6-phosphate dehydrogenase (EC Email dtxtranq(5ictu.edu.vn 1,1.1.4.9), lactate dehydrogenase (EC Ngay nhan bai. 8/2/2015 1.1 1.2.7) va glueose-6-phosphatase Ngay nh^n bai phan bien' 12/2/2015 {G6Pase) (EC 3.1.3.9). Trong do enzvme Ngay chap nhgn dang: 8/3/2015 t ^ u r c s c j i / y ^ u , enzyme
Vietnam Journal of Physiology 19(1), 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 G6Pase la mgt trong nhung enzyme e i n
thiet cho vi§c dieu hoa lugng glucose huyet. Sy tang hogt dgng cua enzyme G6Pase la mgt trong nhung nguyen nhan chinh d i n d i n tang glucose huyet ciia bgnh DTD. Vi vay, kiem soat hoat dgng eiia enzyme G6Pase la mgt trong nhung myc tieu kiem soat benh DTD dugc eae nha khoa hgc quan tam. Cay la dua dugc biit CO dugc tinh ddi vdi benh DTD [1], va la cay la dua cung dugc sd dung d i l u tri bgnh DTD theo kinh nghiem dan gian.
Vifie chdng minh tac dung dieu tri bgnh DTD ciia cao chidt la cay la dda mfit each khoa hpe va ha thdng la can thiet, chinh vi ly do do ma muc tieu cua nghien cuu nay la danh gia higu qua hg glucose huyet ciia cao chiet la eay la dda {Pandanus amaryllifolius) thfing qua ea chd dc e h i hogt dgng ciia enzyme glucose-6- phosphatase d gan va thgn cua chugt b§nh dai thao dudng.
2. D 6 1 T U - O N G V A P H U O N G PHAP NGHIEN CLTU
2.1 Vat ligu nghien c d u
La cay la dua {Pandanus amaryllifolius) duac thu hai d huyen Tra On, tinh VTnh Long. Vat lieu duac rua sgch, cdt nhfi va phai kho den trgng lugng khong ddi Sau khi phai kho, 250 g la dugc ngam trong ethanol 96° trong 72 gid d nhi#t dfi phong. Sau do, phin djch long ben tren dugc loe b i n g g i i y loc Whatman va co dudi dung moi bing he thdng may efi quay (Evaporate) d nhiet do 56°C va ap suat thap, cao ethanol tu la cay la dua dugc trd d 4°C de su dung cho qua trinh thi nghigm,
2.2 Doi t u ^ n g nghien c d u
Chufit nhit (Mus museulus L.) cai khoe mgnh, sgch bgnh, khoang 8 - 1 0 tuin tudi do vifin Pasteur Thanh pho Hd Chi Minh cung cap. Chugt dugc nufii 3 ngay trong phong thi nghigm trudc khi nghien cuu va dugc cho an d i y dii bing thyc pham chuin do Vign Pasteur thanh phd Hd Chi
Minh cung cap, chugt dugc cho udng bing nude sach, Chufit thi nghiem duac nuoi d nhiet do phong va chu ky tdi sang 12/12 gid. Chufit duac can va xac djnh trgng lugng tnjng binh la 26,072 ± 2.608 g; glucose huyit trung blnh la 163,25 ± 27,26 mg/dl dugc chia nglu nhien thanh cac nhom nghien cuu.
2.3 Phuo'ng phap nghien c d u 2.3.1 Phuang phap gay benh dai thao d u d n g va dieu trj benh tren chugt
Chugt dugc gay benh DTD bing each tiem dung dieh alloxan monohydrate (AM) ndng do 130 mg/kg trgng lugng chufiL Chugt dugc xem la benh DTD khi co nfing do glucose huyet > 250 mg/dl, Sau khi chugt ti benh 7 ngay, ehugt benh DTD duge didu tri bang thudc thuong mai glucofast (150 mg/kg trgng lugng/ lan x 2 lln/ngay) hoac cao chill la cay la dua (400 mg/kg trgng lugng/ Idn x 2 lln/ngay) trong thdt gian 20 ngay.
2.3.2 Thiet ke nghien c u u : theo phuo'ng phap mo ta c i t ngang Thi nghigm dugc bfi tri gfim 05 nhom nghien cuu, mdi nhom gdm 06 chufit, cae nhom nghien cuu gdm'
Nhom 1: Chugt binh thudng udng nudc Nhom 2: Chuot binh thudng udng cao ethanol la cay la dua
Nhom 3: Chufii bgnh DTD ufing thufic glucofast
Nhom 4' Chufit benh DTD udng cao ethanol la cay la dda
Nhom 5: Chugt benh DTD khfing dieu In (ufing nudc)
2.3.3 Khao sat hogt dgng enzyme glucose-6-phosphatase a gan va than chugt
Sau 20 ngay thi nghiem, chugt duac giai phSu l l y gan va than de khao sat hoat dgng ciia enzyme G6Pase Gan (hoac than) sau khi giai phlu dugc l l y 500 mg nghiin trong 1 ml dung djch dem sucrose Ignh 0,25 M pH 6,5. Chat ddng nhat dugc ly tam d 4°C vdi tdc dp 3000 vong/phiit
Vietnam Joumal of Physiology 19(1), 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 trong 10 phiiL Phin dich ndi duge s u
dyng dd khao sat hogt dgng eua enzyme G6Pase.
Hogt dfing eOa enzyme G6Pase duge thyc hign theo quy trinh cOa Punitha ef al., [2] CO higu chinh nhu sau: Hdn hgp phan dng gdm 100 pl dung dich sucrose/EDTA 0,1 M pH 6,5 va 200 pl glucose 6 phosphate (G6P) 0,1 M duge pha trong 100 pl dung djch dgm citrate 0.1 M pH 6,5. Sau do hdn hgp phan dng dugc them vao 100 pl djch gan hogc thgn da dugc ddng nhat. Hdn hgp phan dng dugc l i e mgnh sau do dugc u d 37°C trong 30 phi^t. Phan dng dugc ket thuc bang 1 pl TCA/aseorbate (10%/2%. w/v) va tiip tyc duge ly tam vdi tde do 3000 vfing/phut trong 10 phut d nhidt do phdng. Sau khi ly tam, 100 pl djch trich dugc cho vao 400 pl dung dich A va 500 pl nudc khu khoang.
Dung dich A gdm 1,05 g axit ascorbic hoa tan trong 40 ml ciia hon hgp 0.2908 g postassium antymony tartrate, 12 g amoniummolypdate va 140 ml axit sulfunc dugc pha trong 1.3 lit nude khd khoang.
Cudi eung, hogt dfing cua enzyme G6Pase dugc khao sat gian tiep thong qua lugng phosphate vfi ca dugc tgo ra
do sy ehuyen hda G6P thanh glucose va phosphate vo co. Bang phuang phap do quang phd gdc phosphate vfi eg dugc phat hien d budc song 880 nm. Nong dfi phosphate dugc tfnh dya tr§n dudng chuin ndng dg phosphate vo ca.
2.4 X d ly so ligu
Sd ligu dugc x u ly b i n g phan mem Microsoft Excel 2013 va p h i n mdm Minitab 16.0 bang phuang phap phan tich ANOVA va phdp kiem djnh T-test.
3. K £ T Q U A V A B A N LUAN 3.1 Hogt d6ng ciJa enzyme glucose-6- phosphatase trong gan cua chudt t h i nghigm udng cao la cay la dda
Glucose-6-phosphatase la enzyme eo chu yeu trong gan va thgn va la enzyme dong vai trd quan trgng trong vi$e chuyin hda carbohydrate. G6Pase xuc tac qua trinh chuyen hoa G6P thanh glucose va phosphate t y do. Nong dg phosphate vo CO duge tgo thanh do hogt dgng ciia enzyme G6Pase co trong gan chugt thi nghiem duge trinh bay d Bang 1. Ndng dfi phosphate duge tinh dya theo dudng chuan phosphate vd co y = 0,9953x + 0,0016 (R^= 0,9998).
Bang LNdng dp phosphate vo co duge chuyen hoa t d hoat dgng ciia enzyme G6Pase chiet tach t d gan chugt va ndng dg glucose huyet sau 20 ngay d i l u tn.
Nong do glucose huyet (mg/dl) sau 20 ngay dieu trj Nhdm nghien c d u
Nong dd glucose huyet (mg/dl) t r u d c khi dieu trj
Ndng d9 phosphate vo c o
(mg/l) Binh thifang 147,50 ±28,22 146,00 ±25,97 3,5902 ± 0,4256 Binh thuang + cao 1^
DTD + giucofast
165,25 ±13,25 160,00 ±8,52
1,5456 ±0,5876 DTD + cao 1^
306,25 ±98,50
8,50 ± 33,73 305,75 ±61,69125,50 ±29,86
3,2597 ± 0,7014 2,4914 ±0,5722
DTO(kh6ngaieu tri) 429,75 ± 142.50535,50 ±91,66
8,3257 ± 0,4756 Ghi ChO: cac so li$u co • , " * " , * * " tfieo sau trong cCing mgt cot thi khac 6/#f co5% {p<0,05). DTD: chuOt benh DTD. Nhdm chu^ b^nh DTD /chong d?ey tri n=3,
]/ nghia thdng Ite&mLfc c^ nhiim cdn lai n=t
Ket qua ghi nhan (Bang 1 va Hinh 1), cho t h i y lu'o'ng phosphate vo co tao ra tCr hoat dpng cua enzyme GOPase a gan cua nhom chuot binh thuong uong cao i^ \k
1,5456 ± 0,5876 mg/l, thap han nh6m chuOt binh thu6ng u6ng nuoc c i t (3,5902
± 0,4256 mg/l) m6t each khac biet c6 y nghia thong k&. Tuy nhien, s y khac biet
Vietnam Joumal of Physiology 19(1), 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 nay v i n n i m trong gidi hgn cho phep va
thdp han co y nghTa thdng ki so vdi nhom chugt b$nh DTD khong dugc dieu tri (8,3257 ± 0,4756 mg/l).
Ct nhom ehugt bgnh DTD udng cao chiet la cay la dua co ndng dg glucose huydt giam t d 305,75 ± 61,69 mg/dl xudng c6n 125,50 ± 29,6 mg/dl va tuang duong glucose huyet nhdm chugt binh thudng (146,00 ± 25.97 mg/dl). Ket qua thdng k§ cho thay sy giam glucose huyet d nhom chugt benh DTD khac biet cd y nghTa thdng ke. Ket qud (Bang 1) da chdng minh ndng dg phosphate vfi ca cua nhdm chugt bgnh DTD udng cao chidt la dda (2,4914 ± 0,5722 mg/l) t h i p han so vdi nhfim chufit DTD udng glucofast (3,2597 ± 0,7014 mg/l) Dieu nay chung minh cao chiet la cay la dda co kha nang de chd hoat ddng ciia enzyme G6Pase tdt hon thudc thuang mgi glucofast.
Nhom chudt bgnh DTD khfing d i l u trj cd glucose huydt tang lien tyc va x u i t hi#n chugt chet sau 7 ngay Den ngay thd 20 cOa qua trinh didu trj, sd lugng chufit bgnh DTD khfing dugc didu trj c h i t 50%.
Kdt qua (Bang 1) eho t h i y lugng phosphate vo ca tgo ra do boat dgng cua enzyme G6Pase d nhom chufit b^nh DTD khdng didu trj r l l cao (8,3257 ± 0,4756 mg/l) so vdi cae nhom ehugt cfin lai va khac bigt cd y nghTa thdng ke so vdi cae nhfim ehugt binh thudng udng nudc cat, chugt binh thudng udng cao la, ehugt
t>enh DTD udng glucofast, chudt bgnh DTD udng cao la. Ket qua nay da chdng minh lugng phosphate tgo ra cdng nhieu
&ii hogt dgng ciia enzyme G6Pase cang cao va la mgt trong nhung nguyen nhan gay tSng glucose huydt qua muc d chugt.
Kdt qua trong nghien cdu nay cung tuang t y vdi nghifin edu cua Dai Thj Xuan Trang va cs., (2013) [4] khi nghien cdu higu qua uc chd enzyme G6Pase ciia cao chiet cay nhau {Morinda citrifolia). Ngoai ra Zhao et ai, (2011) [5] eung chung minh dugc ring enzyme G6Pase hoat dgng qua muc d chugt benh DTD. la nguyen nhan d i n den ndng dp glucose huyet tang cao. Cao c h i l l la cay la dda c6 kha nang dn ^ n h glucose huyit va giam hogt dfing cua enzyme G6Pase d chudt bfinh DTD trong nghien cuu nay la phii hgp vdi cac nghien cuu trudc day.
T u tat ca cac k i t qua trinh tiay d tren cho ttiay cao chiet la cay la dda c6 kha nang dieu tri benh DTD theo ca c h i dc c h i hoat dgng cua enzyme G6Pase. K i t qua nay bd sung ea sd khoa hgc cho gia tri duge lieu eiia ngudn tai nguyen cay la dua.
3.2 Hoat ddng cua enzyme glucose-6- phosphatase trong thgn cua chugt thi nghigm uong cao la c i y la dda
Thi nghigm khao sat hogt dgng enzyme G6Pase d than dugc thyc hign tuong t y nhu d gan. Ket qua thi nghigm v l hogt dgng ciia enzyme G6Pase d than dugc trinh bay d Bang 2
Bang 2. Ndng dfi phosphate vo ca dugc chuyen hoa tu hogt dgng cua enzyme G6Pase chiet tach t d than chugt va ndng do glucose huydt sau 20 ngay dieu tri.
Nhom nghi§n c d u
Ndng dg glucose huyet (mg/dl) t r u ^ c khi dieu trj
Nong dd glucose huyet (mg/dl) sau 20 ngay dieu trj
Nong do phosphate vo co*
(mg/l)
147,50 ±28,22
146,00 ±25,97 1,4258 ±0,5902 Binh thudng + eao la165,25 ±13,25
160,00 ± 8,52 0,5684 ±0,1133 DTD + Glucofast306,25 ±98,50 198,50 ±33,73 1.5757 ±0,8678
305,75 ±61,69 125,50 ±29,86 0,4519 ±0,1070 DTD + caoia
DTD (khfing didu trj) 429,75 ±142,50 535,50 ±91,66
3,0858 ±0,0231 Ghi chu: cic so Ii0u c6 ', *', '" theo sau trong cung mgt cot Ihi khac biet c6 '^ nghia thong ke d mire 5%(p<0,05). DTD chugt b0nh DTD. Nh6m chugt b$nti DTD khong dieu tri n=3. cac nhom cdn lai n=6.
Vietnam Journal of Physiology 19(1), 3/2015
ISSN: 1859-2376
K i t qua thdng ke (Bang 2) cho t h i ylugng phosphate tgo ra t d hogt dgng cua enzyme tach chiet t u than nhom ehugt binh thudng udng cao la (0,5684± 0,1133 mg/i) khac biet co y nghTa thdng ke so vdi nhom chufit binh thudng udng nudc cdt (1,4258 ± 0.5902 mg/l). Tuy nhien, s y khac bigt nay n i m trong khoang eho phep va t h i p han dang kd so vdi nhom ehugt benh DTD khong d i l u tri (3,0858 ± 0,0231 mg/l). Dieu nay khang djnh, eao chiet la cay la dda khfing anh hudng den hogt dgng eiia enzyme G6Pase d than chugt binh thudng.
Ket qua (Bang 2 va Hinh 1) cho t h i y lugng phosphate vo co d nhom chugt benh DTD ufing cao la cay la dua (0,4519
± 0,1070 mg/l) tgo ra it han va khac biet CO y nghTa thdng ke vdi nhom bgnh DTD uong glucofast (1,5757± 0.8678 mg/l) T u dfi Chung minh eao chiet la dua c6 tac dung de chd hogt dgng cua enzyme G6Pase tdt hon so vdi udng glucofast.
Theo k i t qua eiia thi nghiem khao sat kha nang hoat dgng ciia enzyme G6Pase trong gan va than chufit thi nghigm thi sau phan ung san pham cua nhom nghien
edu nao tgo thanh cd it gdc phosphate vo ea thi kha nang dieu hPa hoat dgng eua enzyme G6Pase tdt han. Ket qua trinh bay d Hinh 1 cho thdy, s y tao thanh gdc phosphate vo ca cua cac nhom sau thi nghiem co s y tuong quan thuan vdi nong do glucose huyet. Nhom chufit bgnh DTD khfing duge dieu tri ham luang glucose huyet cao kem theo lugng gdc phosphate vfi ea dugc tgo ra d j n g cao khac blSt co y nghTa thdng ke so vdi cac nhom cdn lai.
Sau khi eae nhfim chugt benh DTD dugc didu trj khoi benh (glucose huyit nam d gidi hgn binh thudng < 250 mg/d!) thi lugng phosphate vfi ca tgo ra cung thap tuang duang vdi d nhfim chufit binh thudng. K i t qua nay cho t h i y rang, glucose huyet cao la do sy hogt dgng qua muc ciia enzyme G6Pase d gan va than.
Khi sy hoat ddng cua enzyme G6Pase dugc kiem soat b i n g each dc che bdi thudc thuang mgi glucofast hogc eao ethanol la cay la dua thi glucose huyit dugc dn djnh d mdc binh thudng.
600
% 5 0 0 J . 400
" | - 300 I 200 U 100
fc rl. H [fc rk.
8 "St
• Glucose huyet D X o n e dp Pi (Gan) B N o n g d g P i (Tlign) Hinh 1: Ndng do phosphate vo ea chuyen h6a t u hogt dfing cua enzyme G6Pase d gan
va thgn chugt thi nghiem phy thugc vao nong do glucose huyet
Vietnam Journal of Physiology 19(1). 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 Hogt dfing cua enzyme G6Pase d gan
va than duge so sanh dya vao hidu qua uc e h i hoat dgng eua enzyme G6Pase.
Hieu qua hogt dgng eiia enzyme G6Pase dugc tinh mot each tuang ddi bang each eho rang nhom chugt benh DTD khfing dieu tri dugc xem nhu hoat tinh ciia enzyme G6Pase \i cao nhat va tuang duang 100%. Nhu vay, ty le dc chd hoat dfing cDa enzyme G6Pase d gan va than eiia chudt dugc trinh bay trong Bang 3 cho thay, hoat dgng cua enzyme G6Pase
Bang 3. Hieu qua uc c h i hoat dgng ciia enzyme G6Pase tach chiet t u gan hogc than ciia cac nhom chufit thi nghiem
cua nhom chufit bfinh DTD dugc dieu tri bang cao la cay la dda d than (85,35%) cao han d gan (70,11%). So sanh kha nang dieu hoa hoat dong cua enzyme G6Pase eua cao ethanol la cay la dua va thuoc thuang mai glucofast k i t qua eho thay ring cao ethanol la cay la dua co kha nang uc che hoat dong ciia enzyme G6Pase hieu qua han thude thuang mai glucofast d ea gan (60,86%) va than 48,93%).
Nhom nghien ci>u Binh thuang Binh thuong + la DTD + giucofast DTD + la
Hifu qua u>c che hoat ddng cua enzyme G6Pase (%) Gan
56,90 81,39 60,86 70,11
Than 53,79 81,59 48,93 85,35 T u cac ket qua nghien cuu cho t h i y cao
chidt la cay la dua CO tac dung giam hogt tfnh cOa enzyme G6Pase d chugt benh DTD, t u do iam giam ndng do glucose huydt cua chugt bgnh DTD. Day cung la ca sd khoa hoc eho cac nghien cuu thuc nghigm t i i p theo d ngudi. Dong thdi nghien cuu eung eung cap them thong tin CO gia tri khoa hgc cho benh nhan benh DTD dang s u dyng cac logi thao duge de d i l u tn DTD. Ben eanh do, nghien cuu nay gop p h i n khing djnh kiem soat hoat dgng eiia eac enzyme bien duang carbohydrate la hudng mdi cho viec kilm soat ndng do glucose huyit trong d i l u tri bfinh dai thao dudng.
4. K £ T LUAN
Cao chiet ethanol la cay la dua co kha nang hg glucose huyit va uc che hogt dgng enzyme G6Pase d gan va than chugt bgnh DTD. Hi^u qua ciia cac chiet la eay la dda cao hon so vdi thudc dilu tri bgnh DTD thuang mgi glucofast.
TAI Lieu THAM K H A O 1. Do T i t Lgi (1982). Nhung cay thuoc va V! thuoc Viet Nam, NXB Khoa hgc va Ky thuat, Ha Ngi.
2. Punitha. R., Rajendran, K., and Shinvaikar (2005). Alcoholic Stem Extract of Coscinium fenestratum Regulates Carbohydrate Metabolismand Improves Antioidant Status in Streptozocin- Nicotinamide Induced Diebetic Rats.
eCAM. 2(3)375-381.
3. Si M.M., Lou J.S., Zhou C.X., Shen J.N., Wu H.H., Yang B., He Q.J., Wu H.S. (2011). Insulin releasing and alpha- glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo. Journal of Ethnopharmacol 128: 154-159.
4. Dai Thi Xuan Trang, Quach Tu Hue, Vo Thj Nggc Diem, Nguyin Thj Mai Phucng va Bui T i n Anh (2013).
Khao sat anh hudng ciia cao chill ciy nhau {Morinda citrifolia L.) den hoat dgng cua enzyme glucose-6-phosphatase d
Vietnam Journal of Physiology 19(1), 3/2015 ISSN: 1 8 5 9 - 2 3 7 6 ehugt benh tieu dudng. Tgp c/j//</Toa/joc component of Opuntia dillenii T/Tfljng Da//jgc Can Tha. 23b| 115-124. polysaccharides. International Journal of
5. Zhao, L.Y., Lan Q.J., Huang Z.C., Phytotherapy & Phytopharmacology, Ouyang L.J., Zeng F.H. (2011). 9CHIN, 8332
Antidiabetic effect of a newly identified
SUMMARY
EFFECTS OF ETHANOLIC LEAF EXTRACT FROM PANDANUS AMARYLLIFOLIUS PLANT ON ENZYME GLUCOSE-6-PHOSPHATASE IN DIABETIC MICE
Dai Thi Xuan Trang\ Nguyen Thi Ai Lan^
^College of Natural Sciences, Can Tho University Diabetes mellitus is characterized by abnormally high levels of glucose in the blood.
The chronic hyperglycemia is caused by different reasons. Out of these reasons, enzyme glucose-6-phosphatase (GOPase) which Is mainly presented in the livers and kidneys taking an imporiant role in controlling the blood glucose level. The objective is to estimate the activity of enzyme G6Pase in the livers and kidneys of diabetic mice treated with pandan (Pandanus amaryllifolius) ethanolie leaf's extract Mice were induced diabetes by intraperitoneal injection of alloxan monohydrate at concentration of 130 mg/kg body weight. After 7 days of diabetic incubation, mice having diabetes were orally administrated pandan leaf extract (400 mg/kg body weight x 2 times/day) or glucofast medicine (150 mg/kg body weight x 2 times/day). After 20 days of treatment, livers and kidneys of mice were used for determining the inhibitive ability on GOPase. The results showed that pandan leafs extract can significantly reduce the blood glucose concentration in diabetic mice after 20 days of treatment from 305.75 mg/dl to 125.5 mg/dl, a normal blood glucose level eventually. The inhibitive ability on GOPase of ethanolie extract from pandan leaves on livers and kidneys of diabetic mice was dramatically effective. The results also indicated that livers and kidneys of untreated diabetic mice produced 8.3257 mg/l and 3.0858 mg/l inorganic phosphate which significantly differed from those of control mice with 3.5902 mg/l and 1.4258 mg/l, respectively. By treating the diabetic mice with ethanolie extract from pandan leaves, the inorganic phosphate produced in their livers and kidneys significantly decreased with 2.4914 mg/l and 0.4119 mgA, repeetively. In conclusion, the extract of pandan leaves showed the anti diabetic property by inhibiting glucose-6-phosphatase in vivo. '
Keywords: Diabetes, glueose-6-phosphate, glucose-6~phosphatase enzyme, Pandanus amaryllifolius, plant extract.
