Bovine theileriasis in South Africa with special reference to Theileria mutans

(1)

97-98

(2)

99-100

(3)

(4)

(5)

103

(6)

104

(7)

l'-!) I-' f-.' 0 <:.l1

No. of Animal G.pig 1 G.pig 2 G.pig 5 G.pig 3 G.pig 6 G.pig 7 G.pig 8 G.pig 9

s.

40995

s.

40942

s.

41060 G.pig 13 G.pig 14 G.pig 19 G.pig 20 S.40985

TABLE

II. Transmission E. x pe1 ·i ments in Laboratory Animals with Material f rom Cattle Exposed at T z aneen.

Injected from. I

I

Date. Dose. Method. Result. Immunity Test. No. of History. Animal.

s.

40994 Reacted blue tongue virus

I

4. 9.34 1 c.c. blood

I

i.p. Negative ...

-

from Tzaneen " " " " " " " ... - - " " " " " ,. "

•••• 0 ••••••••••••• " , " " " " Febrile reaction 104°F. Killed

-

14.9.34. Spleen showed multiple localized abscesses. " " " " " " The same febrile reaction and - post-mortem symptoms G.pig 6 Killed, showing febrile rc-13. 9.34 " " Negative ... - action - " " " " " " " ... " " " " " " Febrile reaction 104° F. Killed - 19.9.34andinjected G.pigs 13 and 14 G.pig 3 Both showed febrile rc-14. 9.34 :\ .. brain of each i.p. 15-21.9.34 febrile reaction .. Reacted to injectio and 6 actions G.pig 3-6.10.34 febrile reaction with Tzaneen blu tongue virus whic was injected 1.11. 3,

s.

40995 Showed fehrile reaction ... 4.10.34 10 c.c. blood subcut. 7-10.10.34 febrile reaction .. No reaction to injectio of Tzaneen blu tongue virus whic was injected 31.10. 3

s.

40995 ., " ... 4.10.34 " " No reaction ... Reacted to injectio with Tzaneen blc tongue virus whic was injected 31/10/3' G.pig 9 " ,.

...

19. 9.34 0 · 5 c.c. blood i.p. 24-27.9.34 febrile reaction.

-

Killed 2.10. 34. Injected G. pigs 19 and 20 and sheep 40985 " " ,. ... " " " Negative ... - G.pig 13 " ... 25. 9.34 0 · 5 c.c. blood " " ... - - " " " ... " 1 c.c blood " :' ...

,.

" ... 28. 9.34 subcut. 6-8.10.34 febrile reaction No reaction to injectic 105·6°F. 15and16.10.34 of Tzaneen bh febrile reaction 106 ·4° F. tongue virus whic was injected 31/10/3· ----·-------

n e n e h 4 n e n e h

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s

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(8)

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No. of Animal.

s.

41546

s.

41573 G.pig 4 G.pig 11 G.pig 12 G.pig 10 G.pig 15 G.pig 16 G.pig 17 G.pig 18 Rat 1 Rat 2 G.pig 1 G.pig 6 Rat 3 Rat 4 Mouse 1 Mouse 2 Mouse 3 Rat 5 Rat 6 G.pig 7 G.pig 8 Mouse 4 Mouse 5 Mouse 6 Mouse 7

No. of

I

Animal.

s.

40985

s.

40985

s .

40994 G.pig 4 G.pig 6 G.pig 10 ,

c.

5742

c.

5629 ,

c.

5648

lnjecte:! from. History. Reacted to Tza~een b·l~~ tongue Showed febrile reaction ... Killed, showing febrile re- aetion Show<"d febrile reaction ... Bled 6'days afte~' expos~~~ at Tzaneen. Showed febrile reaction ,

,

Bled 7 days after exposure at Tzaneen. Showed fe. brile reaction " " Bled 12 days after exposure at Tzaneen. Showed fe. brile reaction

TABLE

II (continued).

Date. 16.10.34

,

4. 9.34 15. 9.34 13. 9.34 19. 9.34

,

22. 9.34 " 26. 2.35 27. 2.35 " 4. 3.35

Do3e. 5 c.c. blood " 1 c.c. blood 1 c.c., 10 per cent. brain emulsion 1 c.c. blood 0 · 5 c.c blood 1 c.c tiood " 0 ·05 c.c. blood " 1 c.c. blood " 0 · 05 c.c. blood

Method. , i.p. " i.e. i.p. " i.e.

Resul~. Paralized in hindquarters 29.10. 34 and killed No reaction ... . Febrile reaction 105° F. Killed 15.9. 34. Injected G.pigs ll and 12 No reaction ... . Paralyzed in hindquarters 22.9. 34. Destroyed 24/9/34 18-28.9.34 febrile reaction 105° F. killed 28.9.34. Injected G.pigs 15, 16 on 19.9.34; 17 and 18 on 22.9.34 No reaction ... . '' ... . Negative ... . '' ... . Died from peritonitis 21/3/35 Negative ... .

... I

Immunity Test.

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(9)

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No. of Animal. G.pig 2 G.pig 3 G.pig 4 G.pig 5 Mouse 8 Mouse 9 Mouse 10 Mouse 11 Mouse 12 Mouse 13 G.pig 12 G.pig 13 G.pig 14 G.pig 15 G.pig 16 G.pig 17 G.pig 18 G.pig 19 G.pig 20 G.pig 21 G.pig 22 G.pig 23 G.pig 24 G.pig 25 G.pig 9 G.pig 10 G.pig 14 Mouse 15 Mouse 16 Mouse 17 Mouse 18 Mouse 19 Mouse20

No. of Animal.

c.

6033 " C. 6252

c.

6252

c .

5763 " Mice 10 and 11 "

c.

5742

c.

5676 R. appen· diculatus " Mouse 15

TAnLE

II (continued).

Injected from. Date. Dose. History. Bled 5 days after exposure

I

25. 2. 35

I

1 c.c blood at Tzaneen. Showed fe. brile reaction Bled 8'days after ;~posure I 28. 2.35 at Tzaneen. Showed a febrile reaction Brain ~1;mlsion of b~th mice pooled Bled 19' days after ;~posure at Tzaneen. Showed febrile reaction and Koch's bodies " " Unengorged adult ticks collected on veld at Tzaneen " " Brain emulsion in saline ...

" 5. 3.35 " 11. 3.35 28. ·~.35 " 5. 3.35

" 0 ·05 c.c. blood " 0 · 05 c.c. brain emulsion " 1 c.c. blood " 1 c.c of a lO% emulsion " 5 c.c. of a 10

%

emulsion 0 ·05 c.c. 0 ·05 c. c.

Method. i.p. i.e. i.p. i.e.

Result. Died from peritonitis 20/3/35 Negative ... . '' ... . Died from peritonitis 27/3/35 Negative ... . '' ... . Died 5.3.35 ... ·· '' ... . Negative ... . '' ... . Died from peritonitis 16/3/35 Negative ... . Died from peri toni tis 17/3/35 Negative ... . '' ... . Died from peritonitis 16/3/35 Negative ... . Died from Feritonitis 17/3/35 Negative ... . '' ... . Died 5 . 3 . 35 ... .. Negative ... .

Immunity Test. <:;'l t:l t"j ~ 0 0 ~ 0 ;-< -1 > ~ ~ ~ ~ ~ t:l i:'j ~~ ~ t:l q t-3 0 >-< t-3 > ~ t:l ~ 0 ~ i:'j >-< t-3 N

(10)

TIOVINE THEILERIASIS IN S. AFI!IC~ .

.F'rG. J .-Bovine 5648, 1935 Tzaneen Exposure Experiment. Note emaciation, abnormal stature, and particularly the condition of the ears.

·within the space of about 4 weeks this animal in excellent condition, was reduced to this condition.

108

(11)

•

G. DE KOCK, C. J. VAN HEERDEN, R. DU TOI'l', AND W. 0. NEITZ •

Fra. 2.- Liver of Bovine 5648, 1935 Tzaneen Expo~un~ Experiment.. Note extent and distribution of lymphoid hyperplasia.

109

(12)

BOVINE THEILERIASIS IN S. AFRICA.

FIG. 3.- Kidney of Bovine 5641. 1935 Tzaneen Exposure Experiment. Note extent of lymphoid hyperplasia.

110

•

(13)

G. DE KOCK, C. J. VAN HEERDEN, R. DU TOIT, AND W. 0. NEITZ •

. FIG. 4.-Spleen of Bovine 5763, 1935 Tzaneen Exposure Experiment. Note the distribution of the oedema in relation to Malpighian bodies.

Ul

(14)

llOVlNE THEILERIASIS IN S. AFRICA.

Jl'w 5.-Spleen of Bovine 5763, 1935 Tzaneen Exposure Experiment. Higher magnification of Fig. 4, illustrating nature and distribution of oedema and the loss of lymphoid tissue in connection with the Malpighian bodies.

112

(15)

G. DE ROCK, C. J. VAN HEERDEN, R. DU TOI'l', AND W. 0. NEITZ.

Fro. 6.-Lymphoid Gland of clinically healthy bovine 6401, showing the number and distribution of primary and secondary follicles.

113

(16)

BOVINE THEILERIASIS IN S. AFHTCA.

FIG. 7.-Lymphoid Gland of Bovine 5763, 1935 Tzaneen Exposure Experi- ment. Note the changes and loss of the lymphoid nodules.

114

(17)

G. DE 1\0CI\, C. J. VAN HEERDEN, R. DU TOI'f, AND W. 0. NEITZ.

FIG. 8.-Higher magnification of Fig. 7, illustrating the nature and distribution of the oedema and the changes brought about in the nodules.

115

(18)

TIOVINE THEIL~RIASIS Hi S. AFRICA.

J!'IG. 9.-As Fig. 8.

116

(19)

G. DE KOCK, C. J. VAN HEERDEN, R. DU TOIT, AND W. 0. NEITZ.

l<'w. 10.- Prescapular lymph gland. Bovine 6463. Subinoculation Experi-

ment. See App. 4, Table I. Note nature and distribution of the: granules in the lymphocytes.

l<'w. 11.-Higher magnification of Fig. 10.

111

(20)

BOVINE THEILERIASIS TN S. AFHICt\.

Fw. 12.- Prescapular lymph gland. Bovine 6463, Subinoculation Experiment.

Note in the one lymphocyte the intracellular body Rickettsia bov·is referred to and in the other the nature and distribution of the granules.

118

(21)

G: DE IWCI\:, C. J. VAN HEERDEN, R. DU TOIT, AND W. 0. NElTZ.

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CHART I.-Annual returns of smear examination at Allerton Laboratory, Pietermaritzburg.

119

(22)

BOVINE THEILERIASIS IN S. i\FHW.I

Fehr /'far

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CHAitT H.-Temperature chart of bovine 5641 bred at Vryburg and exposed at Tzaneen 1935. Note the nature of the temperature reaction. Refer to Table V.

120

(23)

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CHART IlL- Temperature chart of bovine 5469 bred at Onderstepoort and exposed at Tzaneen 1935. Note the nature of the temperature reaction. Refer to Table V.

121 I

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CHART IV. -Temperature chart of bovine 5523 bred at Vryburg, infested with ticks collected from the veld at Tzaneen. Refer to Table I, Appendix IV.

122

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V .- Temperature chart of splenectomized bovine 4676 bred at Onder- stepoort, infested with ticks collected from the veld at Tzaneen. Refer to Table I, Appendix IV.

123

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CHAltT V I.-Temperature chart of sheep 42330 infested with ticks collected

from the veld. Refer to '!'able III of Appendix IV.

124

(27)

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CHAirr V H.-Temperature chart of splenectomized sheep 35004 infested with ticks collected from veld at Tzaneen. Refer to 'l'able III, Appendix IV.

125 ---

Bovine theileriasis in South Africa with special reference to Theileria mutans

97-98

99-100

103

104

s.

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II. Transmission E. x pe1 ·i ments in Laboratory Animals with Material f rom Cattle Exposed at T z aneen.

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