Effects of photodynamic therapy on A375 Melanoma cells using aluminium phthalocyanine photosensitizer
Summary:
The aim of the study was to explore the effect of Photodynamic Therapy (PDT) using an Aluminium Phthalocyanine Photosensitizer (AlPcS4Cl) at 673 nm and a fluency of 5 J/cm2, in targeting Melanoma cells (A375). This was an in-vitro investigation were dose dependent response of AlPcS4Cl was studied on both A375 and fibroblast (WS1) cell lines and the IC50 calculated from this.
This research is like several other published studies, however, it is said to be imperative to because it helps to identify therapies that can eliminate Metastatic Melanoma (MM) which is reported to have become a major concern due to the potential for cancer relapse and metastasis, as well as the disease being accounted to be resistant to multiple forms of therapy.
The introduction is clearly outlined, the materials and method section were also described in detail and the results well presented.
Briefly, it was reported that significant post-irradiation signs of cell death were detected using microscopy and biochemical assays. An increased release of Lactate Dehydrogenase (LDH) content due to cytotoxicity with increasing doses of AlPcS4Cl was measured. Cell viability testing showed increased damaged cells taking up Trypan Blue Dye. Further, it was said that the study suggested an effective treatment against Melanoma cells.
Generally, the research is not novel in the sense that there are numerous studies of this similar nature reported. Nonetheless, the work can be published as a conference proceeding as the results presented are detailed and well-articulated.
The paper is recommended for publication.
