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Fig. 1. Principle of PCR amplification. A cDNA specific for an enzyme investigated in our group was used as template with theprimer combinations F1s(forward)/F1a(reverse) or R1s(forward)/R1a(reverse) (F=fractal, R=non-fractal) for the generation ofthe 2
Fig. 2. Primer/after shifting due to a short time for temporal evolution of the binding or duplex state
Fig. 3. PCR amplification in dependence on the annealingtemperature. A 500 bp template generated as depicted in Fig.1 was amplified with the non-fractal (top) or fractal (bottom)primer
Fig. 4. Matrix notation for consecutive shifting of primer and template. The matrix shows binding energies upon consecutive shiftingof primer (column) and template (row)
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