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J01130

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FIGURE 1. Mcb1 interacts with the MCM complexharvested. Soluble cell lysates were prepared using the spheroplast method and immunoprecipitated ( in vivo
FIGURE 2. Mcb1 interacts with any of the individual MCM subunits. A, two-hybrid interactions between Mcb1 and Mcm2–7
FIGURE 3. Isolation and phenotype ofarginine finger features, which are characteristic of the canonical MCM pro-teins (which are absent in MCM-BP), are shown for reference.diluted 5-fold, spotted onto YES medium supplemented with MMS, CPT, orHU at the indi
FIGURE 4. Genetic interaction betweenswi7-H4mcb1dilution and incubated at permissive (25 °C) or restrictive (34 and 36 °C) tem-peratures.with the indicated genotypes were spotted onto YES plates and incubated atthe indicated temperatures
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