in
Shizosaccharomyces pombe
D1 Venny Santosa
10.08
Haploid cell:
12-14 μm at division
3.5μm width-constant
Diploid cell:
24μm length at division
5μm width-constant
Facts about S. pombe
Replication fork
S phase
G1 phase
Pre-replicative
Complex (preRC)
MCM2-77
5
6
2
4
3
Initiation of DNA replication
ORC: origin replication complex
MCM: mini chromosome maintenance
CDK: cyclin-dependent kinase
Pol: polymerase
Complex (preRC)
S-Cdk
Cdt1 +Cdc6 Pol //
Conservation of MCM Binding Protein protein in eukaryotes
•
Oryza sativa
•
Homo sapiens
•
Mus musculus
•
Xenopus
•
Drosophila
•
S.pombe
7
DNA replication
Irradiation
Acetyltransferase Eco1/Ctf7
PCNA RFC
Cohesion function in sister chromatid maintenance
8
DNA damage/ replication errors
Irradiation UV-B
Homologous recombination
between sister chromatids
cohesin
PCNA RFC
PCNA: proliferating cell nuclear antigen RFC: replication factor C
Ctf18: chromosome transmission fidelity factor 18
•Ctf18
Overview of DNA replication
Regulation of S phase entry
Cell cycle progression/ DNA replication genes transcription
a,b
9 ORC: origin recognition complexMCM: mini chromosome maintenance CDK: cyclin dependent kinase
CDC: cell division control
CDT: chromatin binding and DNA replication factor
The DNA damage response
recruitment
ATM: ataxia telangiectasia-mutated
ATR: Rad3-related
10
Recruitment and action of ATM
G1
11
Destruction of Cdc25a
Accumulation of
