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Lampiran 1 Media Tumbuh Bakteri
Nama Media Komposisi Jumlah (g) per 1 liter
dH2O Nitrate Mineral Salt
(NMS) MgSO4.7H2O 1.000 CaCl2.6H2O 0.200 KNO3 1.000 KH2PO4 0.272 Na2HPO4 4.000 NH4Cl 4.000 Na2EDTA 0.500 FeSO4.7H2O 0.200 H3BO4 0.030 CoCl2.6H2O 0.020 ZnSO4.7H2O 0.010 MnCl2.4H2O 3.000 Na2MoO4. 2H2O 3.000 NiCl2.6H2O 2.000 CaCl2.2H2O 1.000
Media Denitrifikasi Na asetat 10.000
NaNO3 4.200 (NH4)Cl 0.176 KH2PO4 0.200 K2HPO4.3H2O 0.920 CaCl2.2H2O 0.100 MgSO4.7H2O 0.500 EDTA 0.200 Keterangan:
Isolat bakteri metanotrof diremajakan pada media agar Nitrate Mineral Salt (NMS) + 1% methanol. Isolat Azotobacter dan Azospirillum masing-masing diremajakan pada media agar NMS + 1% sukrosa dan media NMS + 1% asam malat. Isolat bakteri pereduksi N2O diremajakan pada media denitrifikasi.
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Lampiran 2 Sifat Fisik dan Kimia Tanah
Sifat Fisik dan Kimia Tanah Hasil Analisis Nilai *)
pH
H2O 5,60 Agak masam
Tekstur Tanah (%) Pasir 8,45 Liat Debu 21,75 Liat 69,80 Bahan Organik C (%)
(Walkey & Black) 2,39
Sedang Bahan Organik N-total (%)
(Kjeldhal) 0,20 Rendah N (me/100g) NH4OAc, pH 7 Ca 4,56 Rendah Mg 1,15 Sedang K 0,17 Rendah Na 0,68 Sedang KTK 17,28 Sedang P (ppm) HCl 25% 45,90 Tinggi Bray 1 4,70 Rendah
Kejenuhan Basa (%) 37,96 Rendah
N (me/100g) KCl
Al Tidak terukur Sangat rendah
H 0,12 Sangat rendah
Lampiran 3 Perkembangan Tinggi Tanaman padi Percobaan II 0 10 20 30 40 50 60 70 80 90 100 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 Ti n ggi (c m )
Hari Setelah Tanam (hst) NPK dengan N 25% Dosis A1M A1T B1M B1T C1M C1T A4M A4T 0 10 20 30 40 50 60 70 80 90 100 110 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 Ti n ggi (c m )
Hari Setelah Tanam (HST) NPK dengan N 50% Dosis A2M A2T B2M B2T C2M C2T A4M A4T 0 10 20 30 40 50 60 70 80 90 100 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 Ti n ggi (c m )
Hari Setelah Tanam (HST) NPK dengan N 75% Dosis A3M A3T B3M B3T C3M C3T A4M A4T 0 10 20 30 40 50 60 70 80 90 100 110 0 7 14 21 28 35 42 49 56 63 70 77 84 91 98 105 Ti n ggi (c m )
Hari Setelah Tanam (HST) NPK dengan N 100% Dosis A4M A4T B4M B4T C4M C4T