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Lampiran 1
Bahan Kimia Larutan Stock dan Perhitungan ZPT 1. Tabel 13. Bahan-bahan kimia pembuatan larutan stock
No. Bahan kimia Konsentrasi (mgL-1) Unsur yang Terkandung Unsur Makro 1. KNO3 1900 K, N 2. NH4NO3 1650 N 3. CaCl2.2H2O 440 Ca 4. MgSO4.7H2O 370 Mg, S 5. KH2PO4 170 K, P Unsur Mikro 6. MnSO4.4H2O 16,9 Mn, S 7. ZnSO4.7H2O 8,6 Zn, S 8. H3BO3 6,2 B 9. K3 0,83 K, 1 10. Na2MoO4.7H2O 0,250 Mo 11. CoCl2.6H2O 0,025 Co 12. CuSO4.5H2O 0,025 CuS
Unsur Mikro Besi (Fe)
13. FeSO4.7H2O 27,8 Fe
14. Na2EDTA 37,3
2. Perhitungan penambahan zat pengatur tumbuh NAA dalam media MS0
Stok NAA dibuat dengan cara melarutkan 10 mg NAA dengan menggunakan NaOH 1 N, kemudian diencerkan menggunakan aquadest 100 ml. sehingga larutan stock tersebut memiliki konsentrasi 100 ppm.
a. Penambahan volume NAA 0 ppm dalam 1 liter media V1 x M1 = V2 X M2
V1 X 100 = 1000 x 0
V1 = 0 ml
b. Penambahan volume NAA 0,5 ppm dalam 1 liter media V1 x M1 = V2 X M2
V1 X 100 = 1000 x 0,5
V1 = 5 ml/L (untuk ¼ liter = 1,25 ml) c. Penambahan volume NAA 0,5 ppm dalam 1 liter media
V1 x M1 = V2 X M2 V1 X 100 = 1000 x 1
Lampiran 1 (lanjutan)
d. Penambahan volume NAA 1,5 ppm dalam 1 liter media V1 x M1 = V2 X M2
V1 X 100 = 1000 x 1,5
V1 = 15 ml/L (untuk ¼ liter = 3,75 ml) 3. Perhitungan penambahan ekstrak ragi dalam media MS0
a. Penambahan ekstrak ragi 0 mgL-1 dalam 1 liter media sebanyak 0 mgL-1 b. Penambahan ekstrak ragi 400 mgL-1 dalam 1 liter media
Untuk ¼ liter media = ¼ x 400 mg
= 100 mg
c. Penambahan ekstrak ragi 800 mgL-1 dalam 1 liter media Untuk ¼ liter media = ¼ x 800 mg
= 200 mg
d. Penambahan ekstrak ragi 1200 mgL-1 dalam 1 liter media Untuk ¼ liter media = ¼ x 1200 mg
Lampiran 2
Rekapitulasi Hasil Analisi Ragam (Anova) dan Uji lanjut DMRT Tabel 1. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap
waktu muncul tunas eksplan pisang kepok kuning
Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 345.250a 23.017 1.113 0.385 NAA 3 180.250 60.083 2.904 0.050 Ragi 3 59.417 19.806 0.957 0.425 NAA * Ragi 9 105.583 11.731 0.567 0.813 Galat 32 662.000 20.688 Total 48 2958.000
Tabel 2. Pengaruh NAA terhadap waktu muncul tunas eksplan pisang kepok kuning Konsentrasi NAA (ppm) N Subset 1 2 NAA 0 ppm (N0) 12 3.42 NAA 1.5 ppm (N3) 12 5.92 5.92 NAA 1 ppm (N2) 12 7.75 NAA 0.5 ppm (N1) 12 8.42 Sig. 0.188 0.213
Tabel 3. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap jumlah tunas eksplan pisang kepok kuning
Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 80.146a 5.343 2.788 0.007 NAA 3 19.396 6.465 3.373 0.030 Ragi 3 30.729 10.243 5.344 0.004 NAA * Ragi 9 30.021 3.336 1.740 0.120 Galat 32 61.333 1.917 Total 48 467.000
Tabel 4. Pengaruh NAA terhadap jumlah tunas eksplan pisang kepok kuning
Konsentrasi NAA (ppm) N Subset 1 2 NAA 0 ppm (N0) 12 1.58 NAA 1 ppm (N2) 12 2.58 2.58 NAA 1,5 ppm (N3) 12 3.00 NAA 0,5 ppm (N1) 12 3.25 Sig. 0.086 0.275
Lampiran 2 (lanjutan)
Tabel 5. Pengaruh ragi terhadap jumlah tunas eksplan pisang kepok kuning
Konsentrasi Ragi (mg/L) N Subset 1 2 Ragi 0 mg/L (R0) 12 1.75 Ragi 400 mg/L (R1) 12 2.33 Ragi 800 mg/L (R2) 12 2.42 Ragi 1200 mg/L (R3) 12 3.92 Sig. 0.275 1.000
Tabel 6. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap waktu muncul akar eksplan pisang kepok kuning
Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 557.146a 37.143 1.235 0.298 NAA 3 223.229 74.410 2.473 0.079 Ragi 3 43.063 14.354 0.477 0.700 NAA * Ragi 9 290.854 32.317 1.074 0.408 Galat 32 962.667 30.083 Total 48 5909.000
Tabel 7. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap jumlah akar eksplan pisang kepok kuning
Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 5282.667a 352.178 8.682 0.000 NAA 3 4820.333 1606.778 39.612 0.000 Ragi 3 118.500 39.500 0.974 0.417 NAA * Ragi 9 343.833 38.204 0.942 0.504 Galat 32 1298.000 40.563 Total 48 43322.000
Tabel 8. Pengaruh NAA terhadap jumlah akar eksplan pisang kepok kuning
Konsentrasi NAA (ppm) N Subset 1 2 3 NAA 0 ppm (N0) 12 11.08 NAA 0,5 ppm (N1) 12 28.58 NAA 1 ppm (N2) 12 34.25 NAA 1,5 ppm (N3) 12 36.75 Sig. 1.000 1.000 0.344
Lampiran 2 (lanjutan)
Tabel 9. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap waktu muncul daun eksplan pisang kepok kuning
Tabel 10. Kombinasi NAA dan ragi terhadap waktu muncul daun eksplan pisang kepok kuning
Interaksi antar Perlakuan N
Subset for alpha = 0.05
1 2 3 4 5 NAA 0 ppm, Ragi 0 mg/L (N0R0) 3 11.00 NAA 0 ppm, Ragi 800 mg/L (N0R2) 3 11.33 NAA 0 ppm, Ragi 1200 mg/L (N0R3) 3 14.33 14.33 NAA 0,5 ppm, Ragi400 mg/L ( N1R1) 3 16.00 16.00 NAA 1,5 ppm, Ragi 1200 mg/L (N3R3) 3 20.67 20.67 20.67 NAA 1,5 ppm, Ragi 800 mg/L (N3R2) 3 21.67 21.67 21.67 21.67 NAA 1,5 ppm, Ragi 0 mg/L (N3R0) 3 23.33 23.33 23.33 23.33 NAA 0,5 ppm, Ragi 800 mg/L (N1R2) 3 26.33 26.33 26.33 26.33 26.33 NAA 1 ppm, Ragi 1200 mg/L (N2R3) 3 28.00 28.00 28.00 28.00 28.00 NAA 1,5 ppm, Ragi 400 mg/L (N3R1) 3 28.00 28.00 28.00 28.00 28.00 NAA 1 ppm, Ragi 400 mg/L (N2R1) 3 30.33 30.33 30.33 30.33 30.33 NAA 0,5 ppm, Ragi 1200 mg/L (N1R3) 3 35.00 35.00 35.00 35.00 NAA 0,5 ppm, Ragi 0 mg/L (N1R0) 3 39.67 39.67 39.67 NAA 1 ppm. Ragi 0 mg/L (N2R0) 3 41.67 41.67 NAA 0 ppm, Ragi 400 mg/L (N0R1) 3 44.33 NAA 1 ppm, Ragi 800 mg/L (N2R2) 3 46.00 Sig. 0.070 0.052 0.072 0.058 0.063
Tabel 11. Hasil analisis ragam (Anova) uji F penggunaan NAA dan ragi terhadap jumlah daun eksplan pisang kepok kuning
Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 566.813a 37.788 4.132 .000 NAA 3 22.063 7.354 .804 .501 Ragi 3 352.729 117.576 12.856 .000 NAA * Ragi 9 192.021 21.336 2.333 .038 Galat 32 292.667 9.146 Total 48 2953.000 Sumber keragaman Derajat bebas Jumlah kuadrat Kuadrat
tengah F hitung Probabilitas Perlakuan 15 5932.313a 395.487 3.429 0.002 NAA 3 1838.563 612.854 5.314 0.004 Ragi 3 203.729 67.910 0.589 0.627 NAA * Ragi 9 3890.021 432.225 3.748 0.003 Galat 32 3690.667 115.333 Total 48 45539.000
Lampiran 2 (lanjutan)
Tabel 12. Kombinasi NAA dan ragi terhadap jumlah daun eksplan pisang kepok kuning
Interaksi antar Perlakuan N
Subset for alpha = 0.05
1 2 3 NAA 0 ppm, Ragi 400 mg/L (N0R1) 3 3.00 NAA 1 ppm, Ragi 0 mg/L (N2R0) 3 3.00 NAA 0,5 ppm, Ragi 0 mg/L (N1R0) 3 4.00 NAA 1,5 ppm, Ragi 0 mg/L (N3R0) 3 4.00 NAA 0 ppm, Ragi 0 mg/L (N0R0) 3 4.67 NAA 1 ppm, Ragi 400 mg/L (N2R1) 3 4.67 NAA 0,5 ppm Ragi 400 mg/L (N1R1) 3 6.00 NAA 1 ppm, Ragi 800 mg/L (N2R2) 3 6.00 NAA 1,5 ppm, Ragi 400 mg/L (N3R1) 3 6.00 NAA 0 ppm, Ragi 800 mg/L (N0R2) 3 6.33 NAA 0,5 ppm, Ragi 1200 mg/L (N1R3) 3 6.67 6.67 NAA 1,5 ppm, Ragi 800 mg/L (N3R2) 3 6.67 6.67 NAA 0,5 ppm, Ragi 800 mg/L (N1R2) 3 7.33 7.33 NAA 1,5 ppm, Ragi 1200 mg/L (N3R3) 3 8.33 8.33 NAA 1 ppm, Ragi 1200 mg/L (N2R3) 3 12.00 12.00 NAA 0 ppm, Ragi 1200 mg/L (N0R3) 3 17.00 Sig. 0.079 0.060 0.051
Lampiran 3.
Pertumbuhan Eksplan Pisang Kepok Kuning
Gambar 16. Induk plantlet pisang kepok kuning Gambar 17. Pengecekan mikroklimat
Gambar 18. Pertumbuhan tunas eksplan pisang Gambar 19. Pertumbuhan daun eksplan pisang
Gambar 20. Pertumbuhan akar eksplan pisang Gambar 21. Pertumbuhan akar dari bawah
Lampiran 4.
Dokumentasi Kegiatan Penelitian
Gambar 22. Penimbangan gula
Gambar 23. Ragi roti Gambar 24. Penimbangan ragi Gambar 27. Pengambilan larutan Gambar 26. Penuangan aquadest Gambar 28. Pemanasan media Gambar 28. Penuangan larutan Gambar 31. Penuangan media ke botol Gambar 32. Sterilisasi media Gambar 25. Magnetic stirrer Gambar 29. Pemanasan media
Gambar 30. Penuangan media ke gelas ukur
Gambar 33. Penempatan media ke rak kultur
Gambar 37. Pengambilan tunas induk plantlet
Gambar 38. Meletakkan tunas pada petridish
Gambar 39. Proses
micropropagasi tunas
Gambar 40. Meletakkan tunas pada petridish Gambar 41. Penanaman pada media kultur Gambar 42. Wrapping botol kultur Gambar 34. Pengambilan kertas buram Gambar 35. Meletakkan kertas buram pada petridish
Gambar 36. Membuka induk plantlet dari botol kultur
