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Lampiran 7.Hasil pengukuran absorbansi larutan standar TEP Konsentrasi (pmol/ml) Absorbansi
0 0.089 25 0.170 50 0.269 75 0.313 100 0.384 125 0.440 150 0.508 175 0.534 200 0.640 250 0.730
Lampiran 8. Hasil pengukuran absorbansi sampel
Sampel Konsentrasi Absorbansi
Ulangan 1 Ulangan 2 Rata-rata
KT (C1) 0.277 0.522 0.3995 KT (C2) 0.139 0.27 0.2045 KT (C3) 0.134 0.225 0.1795 Tulang A (C1) 0.343 0.325 0.334 Tulang A (C2) 0.206 0.19 0.198 Tulang A (C3) 0.126 0.15 0.138 Tulang B (C1) 0.43 0.369 0.3995 Tulang B (C2) 0.274 0.199 0.2365 Tulang B (C3) 0.21 0.15 0.18
Contoh perhitungan kadar malonaldehida Sampel : Tulang A (C1)
Absorbansi : 0.334
Persamaan kurva standar : y = 0.002x + 0.117 Maka :
y = 0.002x + 0.117 0.334 = 0.002x + 0.117 x = 108.5 pmol/ml
Lampiran 12. Perhitungan Kurva Standar Pengukuran Kapasitas Antioksidan
Kapasitas antioksidan (%) =
Kurva Standar Asam Askorbat
y = 0.0009x + 0.0151 R2 = 0.9964 0 0.2 0.4 0.6 0.8 1 0 200 400 600 800 1000 1200
Konsentrasi Asam Askorbat
(A .K o n tr o l N e g a ti f-A A s a m A s k o rb a t % 100 _ _ _ _ _ x negatif kontrol Absorbansi sampel Absorbansi negatif kontrol Absorbansi