BAB IV. BIAYA DAN JADWAL PENELITIAN
4.2 Jadwal Penelitian
Sub Total 3 (Rp) 0 4. Belanja Honorarium
Item Honor Waktu Satuan Honor/ Total Pajak PPh
Jam 21 22 23 PPn (Rp.) (Rp.) (Rp) (Rp) (Rp) (Rp) - 0 Sub Total 4 (Rp) 0 Total Keseluruhan (Rp) 50.000.000 4.2 Jadwal Penelitian
Penelitian ini akan dilaksanakan sesuai dengan jadwal di bawah ini:
Jenis Kegiatan Bulan ke
1 2 3 4 5 6 7 8 9
Persiapan dan Pembelian bahan kultur Persiapan kultur jamur Tramter versicolor Analisis kemampuan jamur dalam produksi enzim Produksi laccase
Purifikasi parsial dan uji aktifitas enzim Oksidasi biji Cacao menggunakan laccase Analisis Data dan Pembuatan pubikasi Pembuatan laporan akhir
DAFTAR PUSTAKA
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Afoakwa, E. O. 2014. Cocoa Production and Processing Technology. New York : CRC Press. Agustini, L., Irianto, R. S. B., Turjaman, M., dan Santoso, E. 2011. Isolat Dan Karakterisasi Enzimatis Mikroba Lignoselulolitik di Tiga Tipe Ekosistem Taman Nasional. Jurnal Pendidikan Ilmiah dan Konservasi Alam, 8 (2) : 197-210.
Aikpokpodion, P. E. and Dongo, L. N. 2010. Effects of Fermentation Intensity on Polyphenols and Antioxidant Capacity of Cocoa Beans. Int. J. Sustain. Crop Prod. 5(4):66-70
Ainiyah, S., Lisdiyanti, P., Pertiwi, M., dan Nugroho Prasetyo, E. 2014. BIOGROUTING: Produksi Urease Dari Bakteri Laut (Oceanobacillus sp.) Pengendap Karbonat. Jurnal Sains Dan Seni Pomits. 2 (1) : 2337-3520.
Amir, R. M., Anjun, F. M., Khan, M. I., Pasha, I, and Nadeem, M. 2013. Application of Fourier Transform Infrared (FTIR) Spectroscopy For The Identification of Wheat Varieties. Journal Food Sci Technol, 30 (5) : 1018-1023.
ASEAN Standard. 2014. ASEAN Code of Practice for the Prevention and Reduction of Ochratoxin A Contamination in Cocoa Beans. Diakses pada www.koko.gov.my, pada 2 November 2018, pukul 20.00 WIB
Atmaja, M. I. P., Haryadi, dan Supriyanto. 2016. Peningkatan Kualitas Biji Kakao Non Fermentasi Melalui Perlakuan Pendahuluan Sebelum Inkubasi. Jurnal TIDP. 3(1):11–20.
Azhari, A., Falah, S., Nurjanah, L., Suryani, dan Bintang, M. 2014. Delignifikasi Batang Kayu Sengon oleh Trametes versicolor. Current Biochemistry, 1 (1) : 1-10.
Badan Standardisasi Nasional. 2008. Standar Nasional Indonesia Biji Kakao. SNI 2323 : 2008. Bradford, M. M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry. Vol 72, 248-254.
Brijwani, Khushal, Rigdon, A., and Vadlani, P. V. 2010. Fungal Laccase : Production, Function and Application in Food Processing. Enzyme Research, 2010 : 1-11.
Camu, N., Winter, T. D., Addo, S. K., Takrama, J. S., Bernaert, H., and Luc De Vuyst. 2008. Fermentation of cocoa beans: influence of microbial activities and polyphenol concentrations on the flavour of chocolate. Journal of the Science of Food and Agriculture, 88 : 2288–2297. Cristopher, L. P., Yao, B., Ji, and Yun. 2014. Lignin Biodegradation with Laccase-mediator System. Frontiers in Energy Researches, 2(12).
Elwers, S., Zambrano, A., Rohsius, C., and Lieberei, R. 2009. Differences between the content of phenolic compounds in Criollo, Forastero and Trinitario cocoa seed (Theobroma cacao L.). Eur Food Res Technol, 229:937–948.
Fitriana, M. and E. N. Prasetyo. 2016. Biobleaching serat kapas menggunakan laccase Trametes
versicolor. Tesis
Gautam, Ajay, K. 2013. Notes on Wood Rotting Fungi From India : Trametes versicolor – The Turkey Tail. Journal of New Biological Reports, 2(2) : 67 -70
Genovese, M, I. and Lannes, S. C. Comparison of total phenolic content and antiradical capacity of powders and “chocolates” from cocoa and cupuassu. Ciênc. Tecnol. Aliment., Campinas, 29 (4) : 810-814.
Gochev, V.K and A.I Krastanov. 2007. Fungal laccases. Bulg. J. Agric Sci, 13:75-83.
Guerrerro, D. G., Martinez, V. E., Almaraz, and De La Torre, R. 2011. Cultivation of Trametes
versicolor in Mexico. Micologia Aplicada International, 23 (2) : 55-58.
Han, M., Choi, H., and Song, H. 2005. Purification and Characterization of Laccase from the White Rot Fungus Trametes versicolor. The Journal of Microbiology, 43 (6) : 555-560.
Hu, Y., Pan, Z. J., Liao, W., Li, J., Gruget, P., Kitts, D. D., and Lu, X. 2016. Determination of Antioxidant capacity and Phenolic Content of Chocolate by Attenuated Total Reflectance-Fourier Transformed-infrared Spectroscopy. Food Chemistry, 202 : 254-261.
Irshad, M., Asgher, M., Sheikh, M.A., and Nawaz., H. 2011. Purification and Characterization of Laccase Produced by Schyzophylum commune, IBL-06 in Solid State Culture of Banana Stalks. Bioresources, 6 (3) : 2861- 2673Jhan, M. H., Yeh, C. H., Tsai, C, C., Kao, C. T., Chan, C. K., and Hsieh, C. W. 2016. Enhancing the Antioxidant Ability of Trametes versicolor Polysaccharo peptides b an Enzymatic Hydrolysis Process. Molcules, 12.
Madhavi, V., and Lele, S. S. 2009. Laccase ; Properties and Application. Bioresources, 4 (4) : 1694-1717.
Martini, M. H., Figueira, A., Lenci, C. G., and Tavares, D. Q. 2008. Polyphenolic Cells and Their Interrelation With Cotyledon Cells in Seven Species of Theobroma (Sterculiaceae). Revista Brasil. Bot., 31(3) : 425-431.
Mishra, S. K. 2018. Optimization and Characterization of Purified Laccase from Bacterial and Fungal Sources. Department Of Biochemistry And Biochemical Engineering, Jacob Institute Of Biotechnology And Bioengineering, Sam-Higginbottom University Of Agriculture And Technological Sciences. Tesis
Nicolet, Thermo. 2001. Introduction to Fourier Transform Infrared Spectrometry. USA : Thermo Electron Business.
Nugroho Prasetyo, E., Kudanga, T., Ostergaard, L., Rencoret, J., Gutiérrez, A., Del Río, J. C., Santos, J. I., Nieto, L., Jiménez-Barbero, J., Martínez, A. T., Li, J., Gellerstedt, G., Lepifre, S., Silva, C., Kim, S. Y., Cavaco-Paulo, A., Klausen, B. S., Lutnaes B. F., Nyanhongo, G. S., and Georg M. Guebitz. 2010. Polymerization of lignosulfonates by the laccase-HBT
(1-hydroxybenzotriazole) system improves dispersibility. Bioresource Technology, 101 : 5054– 5062.
Nugroho Prasetyo, E., Rodrıguez, R. D., Lukesch, B., Weiss, S., Murkovic, M., Katsoyannos, E., Sygmund, C., Ludwig, R., Nyanhongo, G. S., and Guebitz, G. M. 2014. Laccase–cellobiose dehydrogenase-catalyzed detoxification of phenolic-rich olive processing residues. International Journal Environ Sci Technol, DOI 10.1007/s13762-014-0526-y.
Nyanghongo, G. S., Gomes, J., Gubitz, G., Zvauya, R., Read, J. S., and Steiner, W. 2002. Production of Laccase by a Newly Isolated Strain of Trametes modesta. Bioresource Technology, 84 : 259-263
Osma, J. F., Toca-Herrera, J. L., and Rodrıguez-Couto,. 2010. Uses of Laccases in the Food Industry. Enzyme Research. Article ID 918761.
Peláez, P. P., Guerra, S., and Contreras, D. 2016. Changes In Physical And Chemical Characteristics Of Fermented Cocoa (Theobroma cacao) Beans With Manual and Semi-Mechanized Transfer, Between Fermentation Boxes. Scientia Agropecuaria, 7 (2): 111 – 119. Polaina, J. and MacCabe, A. P. 2007. Industrial Enzyme, Structure, Function and Application. Netherland : Springer.
Pourcel, L., Routaboul, J., Kerhoas, L., Caboche, M., Lepiniec, L., and Debeaujon, I. 2005. TRANSPARENT TESTA10 Encodes a Laccase-Like Enzyme Involved in Oxidative Polymerization of Flavonoids in Arabidopsis Seed Coat. The Plant Cell Preview, www.aspb.org, American Society of Plant Biologist.
Pusat Penelitian Kopi dan Kakao Indonesia. 2015. Kakao : Sejarah, Botani, Proses Produksi, Pengolahan dan Perdagangan. Yogyakarta : UGM Press.
Qin, Z., Yue, Q., Borras-Hidalgo, O and Xinli Liu. 2017. Laccase Enzyme from Trametes
versicolor with a High Decolorizing Ability on Malachite Green. EC Microbiology Research Article, 10,3 : 127-133.
Rameshaiah, G.N., Reddy, M.L. and Jagadish. 2015. Application of Lignolytic Enzyme from a White-Rot Fungus Trametes versicolor. Universal Journal of Environment Reasearch and Technology, 5 (1) : 1 – 7.
Reymond, J. 2006. Enzyme Assay. Germany : WILEY-VCH Verlag GmbH & Co. KGaA
Shraddha, Shekher, R., Sehgal, S., Kamthania, M. and Kumar, A. 2011. Laccase : Microbial Sources, Production, Purification, and Potential Biotechnological Applications. Enzyme Research. Article ID 217861.
Stoilova, I., Krastanov, A., and Stanchev, V. 2010. Properties of Crude Laccase from Trametes
versicolor Produces by Solid Substrate Fermentation. Advance in Biosience and Biotechnology,
1 : 208-215.
Takemori, T., Ito, Y., Ito, M and Yoshama, M. 1992. Flavor and taste improvement of cacao nib by enzymatic treatment. Japan Kokai Tokkyo Koho, JP 04126037 A2.
Tamimi, M. and Herdyastuti, N. 2013. Analysis Functional Groups Using FTIR Spectroscopy of Chitin Variation. Journal of Chemistry, 2(2) : 47-51.
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Vantamuri, A. B. and Kaliwal, B. B. 2015. Production and Optimization of Laccase by Marasmius sp. BBKAV79 in Submerged Fermentation. International Journal of Current Reasearch, 7 (7) : 18308-18314.
Wollgast, J. and Anklam, E. 2000. Review on polyphenols in Theobroma cacao: changes in composition during the manufacture of chocolate and methodology for identification and quantification. Food Research International, 33 : 423-447.
Woo-Sik, J., Min-ji, K., Seong-Yong, C., Young-Bok, Y., Soon-Ja., S., and Hee-Young, J. 2010. Culture Condition for Mycelial Growth of Corious versicolor. Microbiology, 38 (3) : 195-202.
Lampiran 1. Dukungan sarana dan prasarana penelitian
Sarana dan prasarana untuk penelitian semua tersedia di Departemen Biologi, Institut Teknologi Sepuluh Nopember (ITS). Alat yang urgent dan sudah tersedia adalah :
No Nama Alat Lokasi Kondisi
1 Alat gelas ITS Baik
2 Autoclave ITS Baik
3 Inkubator ITS Baik
4 Oven ITS Baik
5 Shaker ITS Baik
6 Spektrofotometer ITS Baik
7 Freeze dryer ITS Baik
8 Laminar air flow ITS Baik
9 Micropipet ITS Baik
10 ICP ITS Baik
11 Sentrifus rpm tinggi ITS Baik
12 Waterbath ITS Baik
13 Vortex ITS Baik
14 Magnetic stirer ITS Baik
15 Water purifier ITS Baik
16 Freezer (-20oC) ITS Baik
17 Oven 200o C ITS Baik
18 Analitical Balance ITS Baik
19 Anaerobic jar ITS Baik
20 Desicator ITS Baik
21 Freeze Dryer ITS Baik
22 Incubator ITS Baik
23 Hot plate ITS Baik
24 Lux sensor ITS Baik
25 Mikroskop cahaya ITS Baik
26 Mikroskop Fotografi ITS Baik
27 pH meter ITS Baik
28 Dan lain-lain ITS Baik
Lampiran 2. Susunan organisasi tim peneliti dan pembagian tugas
No Nama Alokasi Waktu (Jam/ Minggu)Kompetensi Uraian Tugas 1 Dr. techn. Endry
Nugroho Prasetyo, MT.
5 Bioteknologi 1. Mengkoordinasi program 2. Menganalisa data dan
membuat laporan 2 Siti Zullakiah, ST
MT PhD
3 Teknik Kimia Persiapan dan Operasional Bioreaktor
3 Isdiantoni, S.P., M.P. 4 Agronomi Teknik Agronomi 4 Dr. Ida ekawati, MP 4 Teknologi
Hasil Panen
Teknik Pasca Panen
5 Dwi Setyaning Rahayu, S.Si. 7 Mahasiswa S2 Biologi ITS Membantu mempersiapkan teknis penelitian produksi Enzim
Lampiran 3. Biodata Ketua dan Anggota
Ketua Peneliti :
A. Identitas Diri1 Nama lengkap Dr. techn. Endry Nugroho Prasetyo, MT
2 Jenis kelamin L
3 Jabatan fungsional Dosen Tetap
4 NIP/NIK 197310142000121001
5 NIDN 0014107303
6 Tempat dan tanggal lahir Sumenep/10 Oktober 1973
7 E-mail [email protected]
8 Nomor telepon/HP 082141129126
9 Alamat kantor Gedung H, Kampus ITS Sukolilo 10 Nomor telepon/Faks (031) 596 3857
11 Lulusan yang telah dihasilkan
S-1 = 20 orang
12 Mata kuliah yang diampu Genetika, Biokimia dan Metodologi Penelitian B. Riwayat Pendidikan
S1 S2 S3
Nama Perguruan Tinggi
ITB ITS TU Graz Austria
Bidang Ilmu Biologi Lingkungan Bioteknologi
Tahun Masuk-Lulus 1993-1998 2000-2002 2007-2010 Research group : Biomaterial and Enzyme Technology
Office address : Microbiology and Biotechnology. Laboratory, Department of Biology Faculty of Science,
Intitut Teknologi Sepuluh Nopember (ITS) Gedung H 1st Floor, Kampus ITS, Keputih, Sukolilo Surabaya. Indonesia.
Office phone/fax : +62 31 596 3857
Mobile : +62 82 141 129 126
Email : [email protected], [email protected]
Professional experiences
• Head of Research Scientist and Consultant of Plant Biotechnology Reviving Laboratory and Field Agrotechnology. PT Gudang Garam Tbk. Direktorat Produksi Gempol Pasuruan (2015-2018).
• Research Scientist Coordinator, Corporate Social Responsibility, PT. Husky Madura CNOOC Limited (HCML) Sumenep (2015-2016)
• Research Scientist Coordinator in Student Research Development Team (SR&DT) under cooperation between Wismar University Germany, ITS Surabaya and DAAD Germany (2013-2016)
• Research scientist at TU Graz Austria for developing green catalyst to reduce cigarette smoke toxicants in collaboration with British American Tobacco (BAT), Southampton UK (2010-2013)
• Research Scientist at TU Graz Austria for enzymatic bleaching of cotton EU project (2009-2012).
• Junior researcher at TU Graz Austria for developing tailor-made biocatalysts for renewable polymers in European Union Biorenew Project (2007-2010).
• Project Coordinator of Management in SP4 Grant for Institution's Development, at Sepuluh Nopember Institute of Technology (ITS) Surabaya, Indonesia (2002-2006).
• Research scientist at Urban & Life Environmental Unit Research Center, at Sepuluh Nopember Institute of Technology (ITS) Surabaya, Indonesia (1999-2006).
Publications
Key publications1. Nugroho Prasetyo, E, Semlitch, S., Lemmouchi, Y., Nyanhongo, G.S., Guebitz, G.M. Laccase oxidation and removal of toxicants released during combustion processes. Chemophere, (2016) 144, 652-660.
2. Nugroho Prasetyo, E, Rodríguez, R.D., Lukesch, B.Weiss, S., Murkovic, M., Katsoyannos, E.,
Sygmund, C., Ludwig, R., Nyanhongo, G.S., Guebitz, G.M. Laccase–cellobiose dehydrogenase-catalyzed detoxification of phenolic-rich olive processing residues. Int. J. Environ. Sci. Technol. (2015) 12:1343-1352.
3. Thallinger, B., Nugroho Prasetyo, E., Nyanhongo, G.S., Guebitz, G.M. Antimicrobial enzymes: An
emerging strategy to fight microbes and microbial biofilms. (2013) Biotechnology Journal.
8(1):97-109.
4. Nugroho Prasetyo E., Kudanga T., Fischer R.,Eichinger R., Nyanhongo G.S., Guebitz G.M. Enzymatic synthesis of lignin-siloxane hybrid functional polymers (2012)Biotechnology Journal. 7(2):284-92.
5. Silva C, Matamá T, Kim S.Y, Padrão J, Nugroho Prasetyo E, Kudanga T, Nyanhongo G.S, Guebitz G.S, Casal M, Cavaco-Paulo A. Antimicrobial and antioxidant linen via laccase-assisted grafting (2011)Reactive and Functional Polymers, 71( 7), 713-720.
6. Nugroho Prasetyo, E., Kudanga T, J. Rencoret, Gutiérrez, A, del Río, J., Santos, J.I., Nieto, L., Jiménez-Barbero, J., Martínez, A.T., Li, J., Gellerstedt, G., Lepifre, S., Silva, C., Kim, S.Y., Cavaco-Paulo, A., Klausen, B.S., Lutnaes, B.F., Nyanhongo, G.S., Guebitz, G.M. Polymerisation of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) system improves dispersibility(2010)Bioresource Technology.101(14):5054–5062.
Peer review journals
1. Imamsari, M., Koentjoro, M.P., Nurhayati, A.P., Isdiantoni, Nugroho Prasetyo, E. In-vivo
preliminary examination of moringa oleifera leaves extract as antiaging candidate in swiss webster male mice (Mus musculus). (2018) International Journal of Pharmaceutical Sciences and
2. Hidayati, D., Faizah, A., Nugroho Prasetyo, E., Jadid, N., Abdulgani, N. Antioxidant Capacity of
Snakehead Fish Extract (Channa striata) at Different Shelf Life and Temperatures. IOP Conf. Series: Journal of Physics: Conf. Series 1028 (2018) 012021 doi :10.1088/1742-6596/1028/1/012021.
3. Koentjoro, M.P., Nugroho Prasetyo, E., Rahmatullah, A.M. Optimization of keratinase production
by Bacillus SLII-I bacteria in chicken feather waste medium. (2018) ARPN Journal of Engineering
and Applied Sciences. 13:2. 482-488.
4. Larasati, D., Tsurayya, N., Koentjoro, M.P., Nugroho Prasetyo, E. Keratinase from newly isolated
strain of thermophilic Bacillus for chicken feed modification. AIP Conference Proceedings 1854, 020022 (2017); https://doi.org/10.1063/1.4985413.
5. Ermavitalini, D., Sari, I.P., Nugroho Prasetyo, E., Abdulgani, N., Saputro, T.B. Effect of gamma 60Co
irradiation on the lipid content and fatty acid composition of Nannochloropsis sp. Microalgae. AIP Conference Proceedings 1854, 020009 (2017); doi: 10.1063/1.4985400
6. Nugroho Prasetyo, E, Semlitch, S., Lemmouchi, Y., Nyanhongo, G.S., Guebitz, G.M. Laccase oxidation and removal of toxicants released during combustion processes. Chemophere, (2016) 144, 652-660.
7. Nugroho Prasetyo, E, Rodríguez, R.D., Lukesch, B.Weiss, S., Murkovic, M., Katsoyannos, E., Sygmund, C., Ludwig, R., Nyanhongo, G.S., Guebitz, G.M. Laccase–cellobiose dehydrogenase-catalyzed detoxification of phenolic-rich olive processing residues. Int. J. Environ. Sci. Technol. (2015) 12:1343-1352.
8. Nyanhongo GS, Rodríguez RD, Nugroho Prasetyo E,Caparrós C, Ribeiro, C, Sencadas, V, Lanceros-Mendez S, HerreroAcero E, Guebitz GM. Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility. (2013) Reactive and Functional Polymers. 73(10): 1399–1404.
9. Nyanhongo G.S., Nugroho Prasetyo E.,Sygmund, C, Ludwig, R, Guebitz G.M. An antioxidant regenerating system for continuous quenching of free radicals inchronic wounds. European Journal of Pharmaceutics and Biopharmaceutics, (2013) 83(3):396-404.
10. Thallinger, B., Nugroho Prasetyo, E., Nyanhongo, G.S., Guebitz, G.M. Antimicrobial enzymes: An emerging strategy to fight microbes and microbial biofilms. (2013) Biotechnology Journal. 8(1):97-109.
11. Quaranta M, Nugroho Prasetyo E.,Koren K, Murkovic M, Klimant, I, Nyanhongo G.S., Guebitz G.M. Enzyme-based online monitoring and measurementof antioxidant activity using an optical oxygen sensorcoupled to an HPLC system. Analytical and Bioanalytical
Chemistry. (2013) 405(7):2371-7.
12. Nugroho Prasetyo E.,Knes O, Nyanhongo G.S., Guebitz G.M. Developing SyrinOX total antioxidant capacity assay for measuring antioxidants in humans. International Journal of
Experimental pathology.(2013), 94, 25–33.
13. Nugroho Prasetyo E., Kudanga T., Fischer R.,Eichinger R., Nyanhongo G.S., Guebitz G.M. Enzymatic synthesis of lignin-siloxane hybrid functional polymers(2012)Biotechnology Journal. 7(2):284-92.
14. Fu,J., Nugroho Prasetyo, E.,Nyanhongo, GS., Silva, C:S., Cardinale, M., Yu, C., Cavaco-Paulo, A., Guebitz, G:M. Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility (2012) Biocatalysis and Biotransformation. 30( 1): 27-37. 15. Nyanhongo GS, Nugroho Prasetyo E, Herrero Acero E, Guebitz GM. Engineering strategies
for succesful development of functional polymers using oxidative enzymes. Chemical
16. Nugroho Prasetyo E.,Wonisch W, Nyanhongo G.S., Guebitz G.M. A unique two way approach for the validation of total antioxidant capacity of serum samples (2012) European Journal of Clinical Investigation. 42(4):432-8.
17. Silva C, Matamá T, Kim S.Y, Padrão J, Nugroho Prasetyo E, Kudanga T, Nyanhongo G.S, Guebitz G.S, Casal M, Cavaco-Paulo A. Antimicrobial and antioxidant linen via laccase-assisted grafting (2011) Reactive and Functional Polymers, 71( 7), 713-720.
18. Nugroho Prasetyo, E., Kudanga, T., Steiner, W., Murkovic, M., Nyanhongo, G.S., Guebitz, G.M. Enzymatically enriching naringenin with hydroxylated and/or methoxylatedphenolics compounds (2011) Process Biochemistry, 46(4):1019-1024
19. Nugroho Prasetyo, E., Kudanga, T., Steiner, W., Murkovic, M., Nyanhongo, G.S., Guebitz, G.M. Laccase-generated tetramethoxyazobismethylenequinone (TMAMQ) as a tool for antioxidant activity measurement (2010). Food Chemistry. 118: 437–444.
20. Nugroho Prasetyo, E., Kudanga T, J. Rencoret, Gutiérrez, A, del Río, J., Santos, J.I., Nieto, L., Jiménez-Barbero, J., Martínez, A.T., Li, J., Gellerstedt, G., Lepifre, S., Silva, C., Kim, S.Y., Cavaco-Paulo, A., Klausen, B.S., Lutnaes, B.F., Nyanhongo, G.S., Guebitz, G.M. Polymerisation of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) system improves dispersibility(2010)Bioresource Technology.101(14):5054–5062.
21. Nugroho Prasetyo, E., Kudanga, T., Steiner, W., Murkovic, M., Nyanhongo, G.S., Guebitz, G.M. Cellular and plasma antioxidant activity assay using tetramethoxyAzobismethylene Quinone (TMAMQ)(2010)Free Radical Biology & Medicine.49(7):1205-1211.
22. Kudanga,T., Nugroho Prasetyo, E.,Sipilä, J., Nyanhongo, G.S., Guebitz G.M. Chemo-enzymatic functionalisation of lignocellulose materials using oxiranes (2010) Process Biochemistry. 45( 9):1557-1562.
23. Nyanhongo, G. S., Nugroho Prasetyo, E.,Kudanga, T., Guebitz, G.M. Mechanistic insights into laccase mediated functionalization of lignocellulose material Biotechnology and Genetic engineering reviews (2010) 27:305-330.
24. Kudanga,T., Nugroho Prasetyo, E.,Sipilä, J., Nyanhongo, G.S., Guebitz G.M.Reactivity of long chain alkylamines to lignin moieties: Implications on hydrophobicity of lignocellulose materials(2010)Journal of Biotechnology. 149(1-2): 81-87.
25. Kudanga, T., Nugroho Prasetyo, E., Weber, H, Heathcote, C., Jury, S., Widsten, P., Kandelbauer, A., Sipila, J., Nyanhongo, G. S., Guebitz, G.M. Laccase catalyzed coupling of fluorophenols to lignin increases wood surface hydrophobicity (2010)Bioresource Technology. 101(8):2793-2799.
26. Kudanga, T., Nugroho Prasetyo, E.,Sipila, J., Nyanhongo, G. S., Guebitz, G.M. Enzymatic grafting of functional molecules to the lignin model dibenzodioxocin and lignocellulose material (2010)Enzyme and Microbial Technology. 46(3-4) 272-280.
27. Widsten, P., Kandelbauer, A., Heathcote, C., Nyanhongo, G. S., Guebitz, G.M., Nugroho Prasetyo, E., Kudanga, T. Enzymatic surface functionalisation of lignocellulosic materials with tannins for enhancing antibacterial properties (2010)Process Biochemistry.45(7):1072-1081.
28. Kudanga, T., Nugroho Prasetyo, E.,Sipila, J., Nyanhongo, G. S., Guebitz, G.M. Coupling of functional molecules onto lignin model compound dibenzodioxocin. Lenzinger Berichte. (2009) 87: 90-98.
29. Nugroho Prasetyo, E.,Kudanga, T., Steiner, W., Murkovic, M., Nyanhongo, G.S. Guebitz, G.M. Antioxidant activity assay based on laccase-generated radicals. Analytical and
30. Kudanga, T., Nugroho Prasetyo, E., Nyanhongo, G. S., Guebitz, G.M. Coupling of aromatic amines onto syringylglycerol β–guaiacylether using Bacillus SF spore laccase(2009)Journal of Molecular Catalysis B: Enzymatic61(3-4): 143-149.
31. Kudanga, T., Nugroho Prasetyo, E., Sipilä, J., Nousiainen, P., Widsten, P., Kandelbauer, A., Nyanhongo, G.S., and Guebitz, G.M. Laccase-mediated wood surface functionalization. Engineering in Life Sciences. (2008) 8(3): 297-302.
32. Nugroho Prasetyo, E.,Karnaningroem, N. and Nurhatika, S. Application of root zone system reactor for removing domestic wastewater pollutant and its effect on rice (Oryza
sativa. L) blast resistance, (2003) JurnalPurifikasi, Environmental Engineering Department, ITS Surabaya,
Book chapter
1. Nyanhongo GS, Nugroho Prasetyo E,HerreroAcero E, Guebitz GM Advances in the application of oxidative enzymes in biopolymer chemistry and biomaterial research In: Felber and Rosenaueds.“Functional Materials from Renewable Sources”, American Chemical Society Symposium series 1107. (2012) Page 329-349.
2. Nyanhongo G.S., Nugroho Prasetyo, E., Kudanga T., Guebitz, G. Grafting of functional molecules: insights into peroxidase-derived materials. In: E. Torres and M. Ayala,
eds.Biocatalysis based on heme peroxidases” (2010) 1st Edition, Springer, New York. 3. Nyanhongo G.S., Nugroho Prasetyo, E., Kudanga, T., Georg M. Guebitz. Enzymatic
polymer functionalization: advances in laccase and peroxidase derived functional polymers in biofunctionalization of polymers and their applications. In:Nyanhongo, G. S., Steiner, W., Guebitz. G.M. (eds.)“Biofunctionalization of polymers and their applications” An Advances in Biochemical Engineering/Biotechnology (2010) seriesSpringer Verlag Germany.
Patent
Nugroho Prasetyo E, Lemmouchi Y, Nyanhongo GS, Guebitz G (2012) Enzymes in Smoking Article Filters. British American Tobacco UA Disclosure #795, P10011711.
Scientific meeting and conferences
1. Ni Wayan Sutraeni Rahayu, Isdiantoni,. Nugroho Prasetyo. E., Hydroextraction Ketapang Leaves (Terminalia Catappa L.) To Control Ice-Ice Disease For Kappaphycus alvarezii Cultivation. International Conference on Mathematics Science and Education 2016 2. Jayanti, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,. Thermostable Bacillus sp. SK
II–5 Endospores Production International Conference on Mathematics Science and Education 2016
3. Tria Ainur Rosyidah, Awik Puji Dyah Nurhayati, Nugroho Prasetyo. E.,.Biocatalysis of Keratin-Based Wastes A Source of Soluble Protein. International Conference on Mathematics Science and Education 2016
4. Asma’ul Karima, Sri Nurhatika, Nugroho Prasetyo. E.,. Enzymatic Modification of Cellulose Based Materials for Promising Substrate of Bioethanol Production. International Conference on Mathematics Science and Education 2016.
5. Kholilah Nur Hidayah Awik Puji Dyah Nurhayati, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E., Visceral Organ Waste As Substrate For Lipase Production By Bacillus sp. SK II-5 International Conference on Mathematics Science and Education 2016
6. Arida Wahyu Barselia, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,. Enhancing Compost Activator Life Span by Varying Resources of Nitrogen International Conference on Mathematics Science and Education 2016
7. Maharah Huwaina Madini, Isdiantoni, Nugroho Prasetyo. E.,. Dynamic Abundant of Bacterial Related to Ice ice Disease on Seaweed (Kappaphycus alvarezii) International Conference on Mathematics Science and Education 2016
8. Tri Wijayanti Irma Suryani, Maharani Pertiwi Koentjoro, Nurul Jadid, Nugroho Prasetyo. E.,. Variation of Substrat Sources For Aerobic Compost Production Process to Grow Kelor (Moringa Oleifera) Seedling International Conference on Mathematics Science and Education 2016
9. Siska Ayu Wulandari, Isdiantoni, Nugroho Prasetyo. E.,. Stratification Community of Bacteria Related on Seaweed (Kappaphycus alvarezii ) Ice Ice Disease. International Conference on Mathematics Science and Education 2016
10. Kufah Nur Afifah, Nugroho Prasetyo. E.,. Chitosan Production by Bacillus Isolated Fisheries Waste. International Conference on Mathematics Science and Education 2016 11. Hilman Adzim Ekram, Nugroho Prasetyo. E.,. Beef Tenderization Using Bacterial
Collagenase Isolated From Slaughterhouse. International Conference on Mathematics Science and Education 2016
12. Ditya Lasarati, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,. Enzymatic Modification of Chicken Feathers Waste As Livestock Feed Rich in Nutrients. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 15-23.
13. Qintan Istighfarin Atmaja, Nur Shabrina, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,. Optimization Production and Characterization of Chitin Deacetylase by Thermophilic Bacillus Sp. Sk II-5. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 57-64.
14. Yunita Permanasari, Elvian Haning Pramesti, Isdiantoni, Maharani Pertiwi Koentjoro, Nurul Jadid, Endry Nugroho Prasetyo . The Effect of Salicylic Acid and Phenylalanineon the Total Phenolic Acid Contentin Cell Suspension Culture of Moringa oleifera Lam. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 93-101.
15. Elvian Haning Prameisti, Nurul Jadid, Isdiantoni, Maharani Pertiwi, Nugroho Prasetyo. E.,. The Effect of Elicitors (Salicylic Acid and NaCl) on Total Flavonoid and Flavonol Content in Moringa oleifera Lamk. Cell Suspension Culture. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 117-127.
16. Fanindya Citra Ayu Ardian, Isdiantoni, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,. Selection of Natural Antimicrobial in Poteran Island Based Ethnobotany. ISSN: 2460-8238 (2015) pp: 140-149.
17. Sidratu Ainiyah, Endy Nugroho, Puspita Lisdiyanti, Maharani Pertiwi. Biogrouting: Urease Production From Carbonat Presipitation Bacteria (Oceabobacillus sp.). PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 157-166.
18. Rischa Jayanty, Maharani Pertiwi Koentjoro, Endry Nugroho Prasetyo. Preproduction Chitin Deasetilase from Fisheries Waste. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology : From Local to Global. ISSN: 2460-8238 (2015) pp: 229-237.
19. Nur Shabrina, Qintan Istighfarin Atmaja, Isdiantoni, Maharani Pertiwi Koentjoro, Nugroho Prasetyo. E.,.Bacterial Diversity on Red Macroalgae Kappaphycus alvarezii Infected by Ice-Ice Disease. PROCEEDING 1st International Seminar on “Natural Resources Biotechnology