BAB V KESIMPULAN DAN SARAN
V.2 Saran
Sebaiknya dilakukan uji pewarnaan spesifik untuk melihat intensitas pembentukan kolagen dengan pemberian getah tanaman patah tulang (Euphorbia tirucalli).
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47 LAMPIRAN Lampiran 1: Skema Kerja
Luka A Luka B Luka C
Kontrol (Tidak dioles
apa-apa)
Getah Tanaman Patah Tulang
Salep Gentamisin Aklimatisasi selama
2 minggu
Rambut pada punggung dicukur
Pembuatan luka akut
Penyiapan batang tanaman patah tulang
Pengambilah getah tanaman patah tulang Penyiapan hewan
coba
Pengambilan spesimen Hari ke-3
Hari ke-7 Hari ke-14
Mikroskopik
Kolagen Diberikan
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Lampiran 2: Skema Kerja Pembuatan Preparat Histopatologi
Fiksasi Spesimen
Pemotongan Spesimen
Merendam spesimen dengan larutan BNF 10%, perbandingan antara spesimen dan larutan 1:10. Waktu fiksasi minimal 2 hari
Spesimen dipotong dengan ketebalan 0,3-0,5 mm
Disusun kedalam tissue cassette, kemudian dimasukkan kedalam keranjang khusus (basket)
Proses Dehidrasi
Dimasukkan hasil pemotongan kedalam mesin processor otomatis
Diproses selama 18 jam
Vakum
Penghilangan udara dari jaringan dengan menggunakan mesin vakum (59-60°C)
Proses selama 30 menit
Mencetak Parafin
Jaringan spesimen dimasukkan kedalam setiap cetakan
Parafin dituangkan kedalam cetakan, dibiarkan membeku diatas mesin pendingin
Kemudian blok parafin dilepas dari cetakan
Memotong Jaringan
Blok dipootong dengan menggunakan mikrotom dengan ketebalan 3-4 µm
Potongan diletakkan di permukaan dalam floathing bath bersuhu 40°
Irisan dirapikan, diletakkan di kaca object glass dan dimasukkan kedalam inkubator bersuhu 60°
Pewarnaan
Pewarnaan dengan menggunakan pewarna Mayer’s Hematoxylin, kemudian diteteskan 1-2 tetes DPX, tutup dengan deck glass
Preparat Histopatologi
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Lampiran 3: Hasil Analisis Statistik Pembentukan Kolagen Pada Luka Tikus Hari Ke-3
Ranks
Perlakuan N Mean Rank
Kolagen
Kontrol 4 7.50
Salep 4 6.00
Getah 4 6.00
Total 12
Test Statisticsa,b
Kolagen
Chi-Square .688
Df 2
Asymp. Sig. .709
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
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Lampiran 4: Hasil Analisis Statistik Pembentukan Kolagen Pada Luka Tikus Hari Ke-7
Ranks
Perlakuan N Mean Rank
Kolagen
Kontrol 4 4.75
Salep 4 8.00
Getah 4 6.75
Total 12
Test Statisticsa,b
Kolagen
Chi-Square 1.971
Df 2
Asymp. Sig. .373
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
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Lampiran 5: Hasil Analisis Statistik Pembentukan Kolagen Pada Luka Tikus Hari Ke-14
Ranks
Perlakuan N Mean Rank
Kolagen
Kontrol 3 3.50
Salep 3 6.50
Getah 3 5.00
Total 9
Test Statisticsa,b
Kolagen
Chi-Square 2.667
Df 2
Asymp. Sig. .264
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
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Lampiran 6 : Gambar Penelitian
Gambar 6. Pembagian hewan coba sesuai dengan kelompok.
Gambar 7. Persiapan hewan coba untuk proses perlakuan.
Gambar 8. Persiapan alat dan bahan Gambar 9. Tanaman Patah Tulang
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Gambar 10. Pembiusan tikus dengan menggunakan eter.
Gambar 11. Proses pencukuran bulu tikus.
Gambar 12. Hasil pencukuran. Gambar 13. Pembuatan luka dengan alat biopsy punch.
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Gambar 14. Hasil pembuatan luka. Gambar 15. Pemberian salep pada luka tikus.
Gambar 16. Pemberian 1 tetes getah patah tulang pada luka tikus.
Gambar 17. Proses setelah perlakuan.
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Gambar 18. Proses fiksasi sampel selama 48 jam.
Gambar 19. Proses dehidrasi.
Gambar 20. Alat floathing bath/
pemanas.
Gambar 21. Alat pemotongan blok.
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Gambar 22. Proses mencetak blok. Gambar 23. Proses pewarnaan preparat.
Gambar 24. Hasil preparat histopatologi.
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Lampiran 7 : Surat Rekomendasi Persetujuan Etik
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Lampiran 8 : Surat Keterangan Determinasi Tanaman