BAB 5. KESIMPULAN DAN SARAN
5.2 Saran
Perlu dilakukan identifikasi metabolit sekunder utama (senyawa x) hasil isolasi dari ekstrak n- heksana kencur, selanjutnya diujikan proses biotransformasi dengan menggunakan mikroba jenis lainnya atau kondisi inkubasi yang berbeda.
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27 UIN Syarif Hidayatullah Jakarta C. E, Rajendra., Magadum, G. S., Nadaf, M. A., Manjula. 2011. Phytochemical Screening of The Rhizome of Kaempferia Galanga. International Journal of
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28 UIN Syarif Hidayatullah Jakarta Haq, I., Ali, S., Ashraf, H., Butt, A.W., M. A. Qadeer., Shafiq, K., Iqbal, J. 2001. Effect of Mineral Nutrients on The Biosynthesis of Citric Acid by Aspergillus niger UV-6, Using Sucrose Salt Media. Pak. J. Bot. 33: 535-540.
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29 UIN Syarif Hidayatullah Jakarta Mahmoud, Y. A. G., Assawah, S. W., El-Sharkawy, S. H. and Salam, A. A. 2008. Flavone Biotransformation by Aspergillus niger and the Characterization of Two Newly Formed Metabolites. Mycobiology 36(2): 121-133.
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30 UIN Syarif Hidayatullah Jakarta Rao, S. C. V., Rao, R., and Agrawal, R. 2003. Enhanced production of verbenol, a highly valued food flavourant, by an intergeneric fusant strain of Aspergillus niger
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32 UIN Syarif Hidayatullah Jakarta Walker, J. M. and Rapley, R. 2002. Molekuler Biology and Biotechnology. Britain: Athenaeum Pr.
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LAMPIRAN
Lampiran 1. Kerangka Penelitian
Kultur jamur Aspergillus niger dengan penambahan Metabolit Sekunder Utama
(Senyawa x)
Ekstraksi hasil biotransformasi
Hasil biotransformasi dianalisa dengan KLT dan KCKT
Analisis data
Metabolit Sekunder Utama (Senyawa x)
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Lampiran 2. Penyiapan Kristal Metabolit Sekunder Utama (Senyawa x) Yang diisolasi dari Kencur (Kaempferia galanga L.)
Rimpang kencur
segar 4,5 kg
Dibersihkan dari tanah yang menempel dan dicabut akar –
akar yang menempel dengan dicuci dengan air
Dirajang dan dikeringkan dengan diangin-anginkan di udara yang terbuka
Dihaluskan dengan blender Serbuk rimpang kering kencur 754 g Maserasi 3x5 hari dengan n-heksana 2,5 L Filtrasi Filtrat
Dipekatkan dengan vacuum rotary evaporator
Filtrat pekat diendapkan pada suhu kamar
Kristal yang terbentuk disaring
Kristal direkristalisasi dengan n-heksana dan metanol
Kristal Ampas
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Lampiran 5. Hasil perhitungan Rendemen
Berat simplisia awal : 754 g Berat kristal yang didapat : 21,58
% rendemen beratkristalyangdidapat g
beratserbuksimplisiaawal g x 100 %
% rendemen ,
x 100 %
= 2,86 %
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Lampiran 6. Komposisi Medium Yang Digunakan
No. Nama Medium Komposisi Jumlah
1. PDA (Potato Dextrose Agar) Neogen Potatoes infusion Dektrosa Agar Aquadest 4 g 20 g 15 g 1000 mL
2. Czapek- pepton Sukrosa
Glukosa Peptone Water K2HPO4 KCl FeSO4.7H2O Aquadest 15 g 15 g 5 g 1 g 0,5 g 0,01 g 1000 mL
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Lampiran 7. Kencur (Kaempferia galanga L.)
Tumbuhan kencur (Kaempferia galanga L.)
Kencur (Kaempferia galanga L.) yang telah dikeringkan (Sumber : Koleksi Pribadi)
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Lampiran 8. Jamur Aspergillus niger ATCC 6275
Aspergillus niger ATCC 6275
Jamur Aspergillus niger ATCC 6275 Perbesaran 40x (Sumber : Koleksi Pribadi)