BAB V KESIMPULAN DAN SARAN
B. Saran
1. Perlu dilakukan validasi retrospektif terhadap protokol yang digunakan untuk
memperkuat dugaan aktivitas 2-(4’-hidroksi-3’-metoksibenzilidena)
sikloheksana-1,3-dion terhadap protein NF-κB.
2. Perlu dilakukan uji aktivitas senyawa 2-(4’-hidroksi-3’-metoksibenzilidena) sikloheksana-1,3-dion terhadap protein NF-κB secara invitro.
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LAMPIRAN
Lampiran 1. Data Penimbangan StartingMaterial dan Perhitungan Massa Senyawa Hasil Sintesis Secara Teoritis
REPLIKASI I
sikloheksana-1,3-dion 4-hidroksi-3-metoksibenzaldehida kalium hidroksida Berat wadah 0,418 gram 0,408 gram 15,377 gram Berat wadah + zat 0,872 gram 1,046 gram 15,783 gram Berat wadah + sisa 0,421 gram 0,412 gram 15,382 gram Berat zat 0,451 gram 0,634 gram 10,401 gram Jumlah dalam mmol 4,027 mmol 4,171 mmol 07,035 mmol
HO O O H H 3C + O O HO O O O H 3C
4-hidroksi-3-metoksibenzaldehida sikloheksana-1,3-dion 2-(4’-hidroksi-3’-metoksibenzilidena)sikloheksana- 1,3-dion
mula-mula : - 4,171 mmol - 4,027 mmol - bereaksi : - 4,027 mmol - 4,027 mmol + 4,027 mmol sisa : - 0,144 mmol 0 + 4,027 mmol
mol produk yang diperoleh = 4,027 mmol
massa produk yang diperoleh secara teoritis = 4,027 mmol x 246 g/ mol = 990,642 mg
REPLIKASI II
sikloheksana-1,3-dion 4-hidroksi-3-metoksibenzaldehida kalium hidroksida Berat wadah 0,399 gram 0,407 gram 15,377 gram Berat wadah + zat 0,853 gram 1,020 gram 15,737 gram Berat wadah + sisa 0,404 gram 0,413 gram 15,345 gram Berat zat 0,449 gram 0,607 gram 10,392 gram Jumlah dalam mmol 4,009 mmol 3,993 mmol 06,877 mmol
KOH -H2O
HO O O H H 3C + O O HO O O O H 3C
4-hidroksi-3-metoksibenzaldehida sikloheksana-1,3-dion 2-(4’-hidroksi-3’-metoksibenzilidena)sikloheksana- 1,3-dion
mula-mula : - - 3,993 mmol - 4,009 mmol - bereaksi : - 3,993 mmol - 3,993 mmol + 3,993 mmol sisa : 0 - 0,016 mmol + 3,993 mmol
mol produk yang diperoleh = 3,993 mmol
massa produk yang diperoleh secara teoritis = 3,993 mmol x 246 g/ mol = 982,278 mg
REPLIKASI III
sikloheksana-1,3-dion 4-hidroksi-3-metoksibenzaldehida kalium hidroksida Berat wadah 0,218 gram 0,216 gram 15,340 gram Berat wadah + zat 0,678 gram 0,896 gram 15,737 gram Berat wadah + sisa 0,221 gram 0,219 gram 15,345 gram Berat zat 0,457 gram 0,677 gram 10,392 gram Jumlah dalam mmol 4,080 mmol 4,454 mmol 06,877 mmol
HO O O H H 3C + O O HO O O O H 3C
4-hidroksi-3-metoksibenzaldehida sikloheksana-1,3-dion 2-(4’-hidroksi-3’-metoksibenzilidena)sikloheksana- 1,3-dion
mula-mula : - 4,454 mmol - 4,080 mmol - bereaksi : - 4,080 mmol - 4,080 mmol + 4,080 mmol sisa : - 0,374 mmol 0 + 4,080 mmol
mol produk yang diperoleh = 4,080 mmol
massa produk yang diperoleh secara teoritis = 4,080 mmol x 246 g/ mol = 1003,68 mg
KOH -H2O
KOH -H2O
Lampiran 2. Data Penimbangan Crude Product dan Perhitungan Rendemen