Saran

BAB V KESIMPULAN DAN SARAN

C. Saran

a. Diperlukan penelitian lebih lanjut penggunaan induksi pottasium oxonate dalam durasi yang lebih lama sehingga mampu memberikan dampak kerusakan glomerulosklerosis pada mencit model hiperurisemia sekunder.

b. Diperlukan penanganan atau pengawasan mencit selama penelitian untuk menghindari terjadinya stres pada mencit

c. Diperlukan penelitian mengenai pengaruh jus jambu biji merah pada hiperurisemia sekunder terhadap inflamasi

71

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80

Lampiran 1. Biodata Peneliti

BIODATA

1. Nama : Riska Nur Suci Ayu

2. Tempat, Tanggal lahir : Bondowoso, 13 Maret 1993 3. Profesi/Jabatan : - 4. Alamat Kantor Telepon Fax Email : : : : - - - - 5. Alamat Rumah Telepon Fax Email : : : :

Jl. A Yani Belk Artha Karya II RT. 08 RW. 03 Nangkaan Kecamatan Bondowoso Kabupaten Bondowoso, Jawa Timur 0852 3151 5099

-

riskanursuciayu@gmail.com 6. Riwayat Pendidikan di Perguruan Tinggi

No. Institusi Bidang Ilmu Tahun Gelar

1. Politeknik Negeri Jember Gizi Klinik 2016 S.ST

Daftar Karya Ilmiah

No. Judul Forum Ilmiah Tahun

1. Kajian Pembuatan Biskuit Tepung Biji Nangka Dan Tepung Wortel Sebagai Pmt Pemulihan Untuk Balita Gizi Kurang Usia 24-59 Bulan

Skripsi D-IV Gizi Klinik – Politeknik Negeri Jember

2016

Surakarta, 2019

Lampiran 7. Hasil Uji Normalitas Fibrosis Ginjal Mencit

Case Processing Summary

Cases

Valid Missing Total N Percent N Percent N Percent N 8 100,0% 0 0,0% 8 100,0% CNeg 8 100,0% 0 0,0% 8 100,0% CPos 8 100,0% 0 0,0% 8 100,0% O1 8 100,0% 0 0,0% 8 100,0% O2 8 100,0% 0 0,0% 8 100,0% O3 8 100,0% 0 0,0% 8 100,0% Descriptives^a,b Statistic Std. Error CNeg Mean ,13 ,125 95% Confidence Interval for

Mean Lower Bound -,17 Upper Bound ,42 5% Trimmed Mean ,08 Median ,00 Variance ,125 Std. Deviation ,354 Minimum 0 Maximum 1 Range 1 Interquartile Range 0 Skewness 2,828 ,752 Kurtosis 8,000 1,481 CPos Mean ,13 ,125 95% Confidence Interval for

Mean Lower Bound -,17 Upper Bound ,42 5% Trimmed Mean ,08 Median ,00 Variance ,125 Std. Deviation ,354

Minimum 0 Maximum 1 Range 1 Interquartile Range 0 Skewness 2,828 ,752 Kurtosis 8,000 1,481 O1 Mean ,38 ,183 95% Confidence Interval for

Mean Lower Bound -,06 Upper Bound ,81 5% Trimmed Mean ,36 Median ,00 Variance ,268 Std. Deviation ,518 Minimum 0 Maximum 1 Range 1 Interquartile Range 1 Skewness ,644 ,752 Kurtosis -2,240 1,481 O2 Mean ,63 ,183 95% Confidence Interval for

Mean Lower Bound ,19 Upper Bound 1,06 5% Trimmed Mean ,64 Median 1,00 Variance ,268 Std. Deviation ,518 Minimum 0 Maximum 1 Range 1 Interquartile Range 1 Skewness -,644 ,752 Kurtosis -2,240 1,481 a. N is constant. It has been omitted.

Tests of Normality^a,c

Kolmogorov-Smirnov^b Shapiro-Wilk Statistic df Sig. Statistic df Sig. CNeg ,513 8 ,000 ,418 8 ,000 CPos ,513 8 ,000 ,418 8 ,000 O1 ,391 8 ,001 ,641 8 ,000 O2 ,391 8 ,001 ,641 8 ,000 a. N is constant. It has been omitted.

b. Lilliefors Significance Correction c. O3 is constant. It has been omitted.

Lampiran 8. Hasil Uji Kruskal Wallis Fibrosis Ginjal Mencit

Ranks

Perlakuan Mean Rank F_Pembaca2 Normal 68,00 Kontrol Positif 78,50 Kontrol Negatif 99,50 JJBM 5% 99,50 JJBM 10% 89,00 JJBM 20% 68,00

Test Statistics^a,b

F_Pembaca2 Chi-Square 26,090 df 5 Asymp. Sig. ,000 a. Kruskal Wallis Test

b. Grouping Variable: Perlakuan

Lampiran 9. Hasil Uji Mann Whitney Fibrosis Ginjal Mencit

1. Perlakuan Normal – Kontrol Positif

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Normal 11,50 92,00 Kontrol Positif 13,00 208,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 56,000 Wilcoxon W 92,000 Z -1,022 Asymp. Sig. (2-tailed) ,307 Exact Sig. [2*(1-tailed Sig.)] ,653^b a. Grouping Variable: Perlakuan

b. Not corrected for ties.

2. Perlakuan Normal – Kontrol Negatif

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Normal 12,00 96,00 Kontrol Negatif 18,00 432,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 60,000 Wilcoxon W 96,000 Z -2,011 Asymp. Sig. (2-tailed) ,044 Exact Sig. [2*(1-tailed Sig.)] ,124^b a. Grouping Variable: Perlakuan

3. Perlakuan Normal – O1

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Normal 14,50 116,00 JJBM 5% 22,00 704,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 80,000 Wilcoxon W 116,000 Z -2,044 Asymp. Sig. (2-tailed) ,041 Exact Sig. [2*(1-tailed Sig.)] ,109^b a. Grouping Variable: Perlakuan

b. Not corrected for ties.

4. Perlakuan Normal – O2

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Normal 19,50 156,00 JJBM 10% 25,50 1020,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 120,000 Wilcoxon W 156,000 Z -1,573 Asymp. Sig. (2-tailed) ,116 Exact Sig. [2*(1-tailed Sig.)] ,280^b a. Grouping Variable: Perlakuan

5. Perlakuan Normal – O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Normal 28,50 228,00 JJBM 20% 28,50 1368,00 Total Test Statistics^a F_Pembaca2 Mann-Whitney U 192,000 Wilcoxon W 1368,000 Z ,000 Asymp. Sig. (2-tailed) 1,000 Exact Sig. [2*(1-tailed Sig.)] 1,000^b a. Grouping Variable: Perlakuan

b. Not corrected for ties.

6. Perlakuan Kontrol Positif – Kontrol Negatif

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Positif 17,50 280,00 Kontrol Negatif 22,50 540,00 Total Test Statistics^a F_Pembaca2 Mann-Whitney U 144,000 Wilcoxon W 280,000 Z -1,713 Asymp. Sig. (2-tailed) ,087 Exact Sig. [2*(1-tailed Sig.)] ,192^b a. Grouping Variable: Perlakuan

7. Perlakuan Kontrol Positif – O1

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Positif 20,50 328,00 JJBM 5% 26,50 848,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 192,000 Wilcoxon W 328,000 Z -1,778 Asymp. Sig. (2-tailed) ,075 a. Grouping Variable: Perlakuan

8. Perlakuan Kontrol Positif – O2

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Positif 26,00 416,00 JJBM 10% 29,50 1180,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 280,000 Wilcoxon W 416,000 Z -1,021 Asymp. Sig. (2-tailed) ,307 a. Grouping Variable: Perlakuan

9. Perlakuan Kontrol Positif- O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2

Kontrol Positif 35,50 568,00 JJBM 20% 31,50 1512,00

Test Statistics^a

F_Pembaca2 Mann-Whitney U 336,000 Wilcoxon W 1512,000 Z -2,469 Asymp. Sig. (2-tailed) ,014 a. Grouping Variable: Perlakuan

10. Perlakuan Kontrol Negatif – O1

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Negatif 28,50 684,00 JJBM 5% 28,50 912,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 384,000 Wilcoxon W 912,000 Z ,000 Asymp. Sig. (2-tailed) 1,000 a. Grouping Variable: Perlakuan

11. Perlakuan Kontrol Negatif – O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Negatif 35,00 840,00 JJBM 10% 31,00 1240,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 420,000 Wilcoxon W 1240,000 Z -1,051 Asymp. Sig. (2-tailed) ,293 a. Grouping Variable: Perlakuan

12. Perlakuan Kontrol Negatif – O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 Kontrol Negatif 45,50 1092,00 JJBM 20% 32,00 1536,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 360,000 Wilcoxon W 1536,000 Z -4,504 Asymp. Sig. (2-tailed) ,000 a. Grouping Variable: Perlakuan

13. O1 – O2

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 JJBM 5% 39,00 1248,00 JJBM 10% 34,50 1380,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 560,000 Wilcoxon W 1380,000 Z -1,136 Asymp. Sig. (2-tailed) ,256 a. Grouping Variable: Perlakuan

14. O1 – O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2

JJBM 5% 49,50 1584,00 JJBM 20% 34,50 1656,00

Test Statistics^a

F_Pembaca2 Mann-Whitney U 480,000 Wilcoxon W 1656,000 Z -4,573 Asymp. Sig. (2-tailed) ,000 a. Grouping Variable: Perlakuan

15. O2 – O3

Ranks

Perlakuan Mean Rank Sum of Ranks F_Pembaca2 JJBM 10% 50,50 2020,00 JJBM 20% 39,50 1896,00 Test Statistics^a F_Pembaca2 Mann-Whitney U 720,000 Wilcoxon W 1896,000 Z -3,658 Asymp. Sig. (2-tailed) ,000 a. Grouping Variable: Perlakuan

Lampiran 10. Hasil Uji Kruskal Wallis Glomerulosklerosis Mencit

Ranks

Perlakuan N Mean Rank Glomerulosklerosis_2 1 8 24,50 2 8 24,50 3 8 24,50 4 8 24,50 5 8 24,50 6 8 24,50 Total 48

Test Statistics^a,b

Glomeruloskler osis_2 Chi-Square ,000 df 5 Asymp. Sig. 1,000 a. Kruskal Wallis Test

Lampiran 11. Hasil Uji Normalitas Ekspresi Protein TGF-β Mencit

Case Processing Summary

Perlakuan Cases

Valid Missing Total N Percent N Percent N Percent TGF_Beta_2 Normal 8 100,0% 0 0,0% 8 100,0% Kontrol Negatif 8 100,0% 0 0,0% 8 100,0% Kontrol Positif 8 100,0% 0 0,0% 8 100,0% JJBM 5% 8 100,0% 0 0,0% 8 100,0% JJBM 10% 8 100,0% 0 0,0% 8 100,0% JJBM 20% 8 100,0% 0 0,0% 8 100,0% Descriptives

Perlakuan Statistic Std. Error

TGF_Beta_2

Normal

Mean ,88 ,125 95% Confidence Interval for

Mean Lower Bound ,58 Upper Bound 1,17 5% Trimmed Mean ,92 Median 1,00 Variance ,125 Std. Deviation ,354 Minimum 0 Maximum 1 Range 1 Interquartile Range 0 Skewness -2,828 ,752 Kurtosis 8,000 1,481 Kontrol Negatif Mean 2,50 ,267 95% Confidence Interval for

Mean Lower Bound 1,87 Upper Bound 3,13 5% Trimmed Mean 2,56 Median 3,00 Variance ,571 Std. Deviation ,756

Minimum 1 Maximum 3 Range 2 Interquartile Range 1 Skewness -1,323 ,752 Kurtosis ,875 1,481 Kontrol Positif Mean 2,25 ,164 95% Confidence Interval for

Mean Lower Bound 1,86 Upper Bound 2,64 5% Trimmed Mean 2,22 Median 2,00 Variance ,214 Std. Deviation ,463 Minimum 2 Maximum 3 Range 1 Interquartile Range 1 Skewness 1,440 ,752 Kurtosis ,000 1,481 JJBM 5% Mean 2,13 ,227 95% Confidence Interval for

Mean Lower Bound 1,59 Upper Bound 2,66 5% Trimmed Mean 2,14 Median 2,00 Variance ,411 Std. Deviation ,641 Minimum 1 Maximum 3 Range 2 Interquartile Range 1 Skewness -,068 ,752 Kurtosis ,741 1,481 JJBM 10% Mean 2,25 ,250 95% Confidence Interval for

Mean

Lower Bound 1,66 Upper Bound 2,84

5% Trimmed Mean 2,28 Median 2,00 Variance ,500 Std. Deviation ,707 Minimum 1 Maximum 3 Range 2 Interquartile Range 1 Skewness -,404 ,752 Kurtosis -,229 1,481 JJBM 20% Mean 2,50 ,267 95% Confidence Interval for

Mean Lower Bound 1,87 Upper Bound 3,13 5% Trimmed Mean 2,56 Median 3,00 Variance ,571 Std. Deviation ,756 Minimum 1 Maximum 3 Range 2 Interquartile Range 1 Skewness -1,323 ,752 Kurtosis ,875 1,481 Tests of Normality

Perlakuan Kolmogorov-Smirnov^a Shapiro-Wilk Statistic df Sig. Statistic df Sig. TGF_Beta_2 Normal ,513 8 ,000 ,418 8 ,000 Kontrol Negatif ,371 8 ,002 ,724 8 ,004 Kontrol Positif ,455 8 ,000 ,566 8 ,000 JJBM 5% ,327 8 ,012 ,810 8 ,037 JJBM 10% ,263 8 ,109 ,827 8 ,056 JJBM 20% ,371 8 ,002 ,724 8 ,004 a. Lilliefors Significance Correction

Lampiran 12. Hasil Uji Kruskal Wallis Ekspresi Protein TGF-β Mencit

Ranks

Perlakuan Mean Rank TGF_Beta Normal 11,94 Kontrol Negatif 101,06 Kontrol Positif 80,38 JJBM 5% 74,25 JJBM 10% 83,19 JJBM 20% 101,06 Total

Test Statistics^a,b

TGF_Beta Chi-Square 31,870 df 5 Asymp. Sig. ,000 a. Kruskal Wallis Test b. Grouping Variable: Perlakuan

Lampiran 13. Hasil Uji Mann Whitney Ekspresi Protein TGF-β Mencit

 Perlakuan Normal – Kontrol Negatif

Ranks

Perlakuan Mean Rank Sum of Ranks TGF_Beta Normal 5,38 43,00 Kontrol Negatif 16,06 257,00 Test Statistics^a TGF_Beta Mann-Whitney U 7,000 Wilcoxon W 43,000 Z -3,738 Asymp. Sig. (2-tailed) ,000 Exact Sig. [2*(1-tailed Sig.)] ,000^b a. Grouping Variable: Perlakuan

b. Not corrected for ties.

 Perlakuan Normal – Kontrol Positif

Ranks

Perlakuan Mean Rank Sum of Ranks TGF_Beta Normal 4,50 36,00 Kontrol Positif 20,50 492,00 Test Statistics^a TGF_Beta Mann-Whitney U ,000 Wilcoxon W 36,000 Z -4,654 Asymp. Sig. (2-tailed) ,000 Exact Sig. [2*(1-tailed Sig.)] ,000^b a. Grouping Variable: Perlakuan

 Perlakuan Normal – O1

Ranks

Perlakuan Mean Rank Sum of Ranks TGF_Beta Normal 6,25 50,00 JJBM 5% 24,06 770,00 Test Statistics^a TGF_Beta Mann-Whitney U 14,000 Wilcoxon W 50,000 Z -4,189 Asymp. Sig. (2-tailed) ,000