Saran

BAB V SIMPULAN DAN SARAN

5.2 Saran

Berdasarkan hasil penelitian yang ada, maka disarankan bagi penelitian selanjutnya:

1. Melakukan penelitian lebih lanjut tentang pengaruh konsentrasi ekstrak tanaman kubis terhadap bakteri Escherichia coli dengan menggunakan konsentrasi yang berbeda dari penelitian ini.

2. Melakukan penelitian lebih lanjut tentang kerja antibakteri ekstrak tanaman kubis terhadap bakteri

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DAFTAR PUSTAKA

1. Mohammad J., Sri SYT., et all. Buku Ajar Gastroenterologi-Hepatologi, Jilid 1. Jakarta: Badan Penerbit IDAI. 2010. hal. 87-88.

2. International Vaccine Access Center (IVAC). Pneumonia and Diarrhea Progress Report 2013. Johns Hopkins Bloomberg School of Public Health. 2013. hal. 1-2.

3. Sudoyo, Aru W., dkk. Buku Ajar Ilmu Penyakit Dalam. Jilid 1 edisi IV. Dalam: Simadibrata K, M., & Daldiyono. Diare Akut. Jakarta: Interna Publishing Pusat Penerbitan Ilmu Penyakit Dalam. 2009. hal. 548-549.

4. Bakti Husada. Riset Kesehatan Dasar (RISKESDAS 2013). Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan RI. 2013. hal. 67 & 72

5. Brook GF., Janet SB., et all. Mikrobiologi Kedokteran Jawetz. Ed. 25. Jakarta: EGC. 2010.

6. Suwando S. Jurnal Bahan Alam Indonesia. Skrining Tumbuhan Obat yang Mempunyai Aktivitas Antibakteri Penyebab Karies Gigi dan Pembentukan Plak. ISSN 1412-2855 Vol. 6, No. 2, Januari 2007.

7. Dalimartha, S. Atlas Tumbuhan Obat Indonesia. Jilid 2. Jakarta: PT. Pustaka Pembangunan Swadaya Nusantara. 2000. hal. 115-119

8. Tahira, Z., Riffat, F., et all. In-Vitro Assessment of Antibacterial Activity of Methanol Extract of Brassica Oleracea against Selected Bacterias. JLUMHS September-December Vol 12: No. 03. 2013.

9. Jaiswal, AK., Rajauria, G., et all. Phenolic Composition, Antioxidant Capacity and Antibacterial Activity of Selected Irish Brassica Vegetables. Natural Product Communications Vol. 6 No. 0. 2011.

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10. Plant Database. Brassica oleracea L. (cabbage). Natural Resources Conservation Service. United States Department of Agriculture. Diakses: http://plants.usda.gov/core/profile?symbol=BROL, tanggal: 29/07/2014.

11. Utama, CS., Mulyanto, A. Potensi Limbah Pasar Saytir Menjadi Starter Fermentasi. Universitas Diponegoro Semarang. Jurnal Kesehatan Vol. 2 No. I Juni 2009.

12. Vincent, & Yamaguchi. Sayuran Dunia 2: Prinsip, Produksi dan Gizi. Ed. 2. Bandung: ITB. 1998.

13. Rokayya, S., Li, CJ., et all. Cabbage (Brassica oleracea L. var. capitata) Phytochemicals with Antioxidant and Anti-inflammatory Potential. Asian Pacific Journal of Cancer Prevention, Vol 14, 2013.

14. Jahangir, M. Stress response and health affecting compounds in Brassicaceae: Health-affecting compounds in Brassicaceae. Comp. Rev. Food Sci. Food Saf. 8; 1 – 43. 2009.

15. Ogbede, S.C., Saidu, A.N., Kabiru, A.Y. Phytochemical Compositions, Antihyperlipidemic And Hepatoprotective Effects Of Brassica Oleracae Var. Capitata L. Leaf Extracts On Triton-Induced Hyperlipidemic Rats. International Journal of Medical Science and Clinical Inventions Vol 1 issue 6 page no. 345-351 ISSN: 2348-991X. 2014.

16. Hafidh, RR., Abdulamir, AS., et all. Inhibition of Growth of Highly Resistant Bacterial and Fungal Pathogens by a Natural Product. The Open Microbiology Journal, 5, 96-106. 2011.

17. Kar, Ashutosh. Farmakognosi & Farmakobioteknologi. Ed. 2. Volume 3. Jakarta: EGC. 2013. hal. 846-849.

18. Hafidh RR., Abdulamir AS., Abu Bakar F. Phenotype microarray profiling of the antibacterial activity of red cabbage. Functional Foods in Health and Disease, 2(6):212-227. 2012.

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19. Correa, CB., Martin, JGP., et all. Antilisterial activity of broccoli stems (Brassica oleracea) by flow cytometry. International Food Research Journal 21(1): 395-399. 2014.

20. Integrated Taxonomic Information System (ITIS). Taxonomic Hierarchy.

Escherichia coli: Taxonomic Serial No.: 285 Diakses:

http://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_valu v=285&print_version=PRT&source=to_print, tanggal: 29/07/2014.

21. Misnadiarly, Djajaningrat, H. Mikrobiologi untuk Klinik dan Laboratorium. Jakarta: Rineka Cipta. 2014. hal. 55-56.

22. Pratiwi, ST. Mikrobiologi Farmasi. Jakarta: Penerbit Erlangga. 2008.

23. Bauer AW., Kirby, WMM., Sherris, JC., Truck, M. Antibiotic Susceptibility Testing by a Standardized Single Disc Method. AM J Clin Pathol. 1966.

24. Cadidjah. Uji Aktivitas Antibakteri Ekstrak Diklorometan dan Asetil Asetat Daun Bintaro (Cerbera manghas, linn) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Skripsi. Jurusan Farmasi FKIK UIN Jakarta, Tangerang. 2009.

25. Cetin-Karaca, Hayriye. Evaluation Of Natural Antimicrobial Phenolic Compounds Against Foodborne Pathogens. Tesis. University of Kentucky, USA. 2011. hal. 13-19.

26. Widiatmojo, Hutomo. Uji Potensi Antibakteri Minyak Atsiri Daun Kecombrang (Nicolaia speciosa Horan) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Skripsi. Jurusan Farmasi FKIK UIN Jakarta, Tangerang. 2009.

27. Rinawati, ND. Daya Antibakteri Tumbuhan Majapahit (Crescentia cujete L.) Terhadap Bakteri Vibrio alginolyticus. Institut Teknologi Sepuluh Nopember.

36

LAMPIRAN

Lampiran 1 Hasil Identifikasi/Determinasi Bahan Uji

37

Lampiran 2 Sertifikasi Pengujian Ekstrak Kubis

38

Lampiran 3 Data Hasil Uji Statistik

UJI DESKRIPTIF DAN NORMALITAS DATA

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

zona_hambat 21 100.0% 0 .0% 21 100.0%

Descriptives

Statistic Std. Error

zona_hambat Mean 11.7143 1.56623

95% Confidence Interval for Mean Lower Bound 8.4472 Upper Bound 14.9814 5% Trimmed Mean 11.6270 Median 11.0000 Variance 51.514 Std. Deviation 7.17735 Minimum .00 Maximum 25.00 Range 25.00 Interquartile Range 7.00 Skewness .354 .501 Kurtosis .339 .972 Tests of Normality Kolmogorov-Smirnov^a Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

zona_hambat .254 21 .001 .870 21 .010

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UJI DESKRIPTIF DAN NORMALITAS DATA YANG DI

TRANSFORMASI

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

zona_hambat2 18 85.7% 3 14.3% 21 100.0%

Descriptives

Statistic Std. Error

zona_hambat2 Mean 1.1065 .03671

95% Confidence Interval for Mean Lower Bound 1.0291 Upper Bound 1.1840 5% Trimmed Mean 1.0988 Median 1.0414 Variance .024 Std. Deviation .15575 Minimum .95 Maximum 1.40 Range .44 Interquartile Range .20 Skewness 1.071 .536 Kurtosis -.192 1.038 Tests of Normality Kolmogorov-Smirnov^a Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

zona_hambat2 .329 18 .000 .795 18 .001

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UJI KRUSKAL-WALLIS

Ranks

Uji_konsentrasi N Mean Rank

zona_hambat ektrakkubis15mg/ml 3 5.00 ekstrakkubis50mg/ml 3 8.00 ekstrakkubis60mg/ml 3 12.50 ekstrakkubis80mg/ml 3 12.50 ekstrakkubis100% 3 17.00 Amoksisilin 3 20.00 etanol96% 3 2.00 Total 21

Test Statistics^a,b

zona_hambat

Chi-Square 20.000

Df 6

Asymp. Sig. .003

a. Kruskal Wallis Test b. Grouping Variable: Uji_konsentrasi

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Lampiran 4 Alat dan Bahan

Alat penelitian Autoclave

Inkubator Vortex

42

Lampiran 5

DAFTAR RIWAYAT HIDUP

Nama : Lara Sofhy Wahyuni

Tempat, Tanggal Lahir : Rumbai, 27 Oktober 1992

Alamat : Jl. Balam sakti, panam, Pekanbaru

Email : lara_sw@ymail.com

No.Telpon : 085313336082

Riwayat Pendidikan

1. 1997-1998 : TK IT Mutiara Duri 2. 1998-2004 : SD IT Mutiara Duri

3. 2004-2007 : SMP Swasta Cendana Duri 4. 2007-2010 : SMA swasta Cendana Duri

5. 2010-2011 : Psikologi Universitas Tarumanegara Jakarta 6. 2011-Sekarang : Program Studi Pendidikan Dokter, Universitas