NURLISA ALIAS BUTET
SEKOLAH PASCASARJANA INSTITUT PERTANIAN BOGOR
BOGOR 2013
DAFTAR PUSTAKA
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Lampiran 1. Sekuen nukleotida gen -aktin dari Anadara granosa dan bivalvia lainnya
#MEGA
!Title mega aktin bivalvia_NT; !Format
DataType=Nucleotide NSeqs=8 NSites=374
Identical=. Missing=? Indel=-; !Domain=Data;
#A._granosa_0 ATGTCTGGGG AAGATGATGT AGCCGTTTTT GTTGTTGACA AAGGGTTTGG CATGTGCAAG #A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ... ... ... ... ... ... #A._granosa_2/48 ... ... ... ... ... ... #A._granosa_10/24 ... ... ... ... ... ... #M._yessoensis ... ... ...C...G ...A.... .T..A.CC.. T... #H._cumingii ... ... ...C...A ...G.... .T..A.CC.. T... #A._granosa_0 GCCGGCTTTG CAGGGGACGA TGCCCCCCGG GCTGTTTTTC CTTTCATCGT GGGGGGGCCC #A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ... ... ... ... ... ....C... #A._granosa_2/48 ... ... ... ... ... ....C... #A._granosa_10/24 ...T ... ... ...T... ...A... ....C... #M._yessoensis ...T..C. .T..A... ...T..T..T ..C..C..C. .C.C... C..AA.A... #H._cumingii ...T.... .T..A..T.. ...A..AA.A ..C..G.... .A.C... ....A.A...
Lampiran 1. Lanjutan
#A._granosa_0 AGGCACCAGA GCATGATGTG GGGCATGCTT CAGATGGATT TCTATTTGGG CAATGAGGCC #A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ... ...G.... ... ... ... ... #A._granosa_2/48 ... ...G.... ... ... ... ... #A._granosa_10/24 ... ... ... ... ... ... #M._yessoensis C.T..T...G .AG...GT C..T...GGA ....A...CA G....G.C.. TG... #H._cumingii ..A..T...G .TG...GT T..T...GGA ....A...CA G....G.C.. AG.C..A... #A._granosa_0 CAAAGAAAAA AAGGAATCCT GACCCTAAAG TACCCCATGG ACAACGGCAT CTTCACCAAC #A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ... ... ... ... ... ... #A._granosa_2/48 ... ... ... ... ... ... #A._granosa_10/24 ... ... ... ... ... ... #M._yessoensis ..G..C..G. GG..T... A...C... ...C. .AC....T.. .G....A... #H._cumingii ..G..C..G. G...T... C...C... ..T..T..T. .AC....A.. TG.A..A... #A._granosa_0 TGGAACAACA TGAAAAAAAT CTGGCACCAC ACCTTCTACA ATGAGTTGCG TGTGGTTCCC #A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ...A. ... ... ... ... ... #A._granosa_2/48 ...A. ... ... ... ... ... #A._granosa_10/24 ...A. ... ... ... ... ... #M._yessoensis ...G..G.T. ..G.G..G.. T...T... ... .C...C.C.. ...A.C.... #H._cumingii ...G.TG.T. ..G.G..G.. ...T... ... ....AC.C.. ...CC..T
Lampiran 1. Lanjutan
#A._granosa_0 GAGGAGCACC CCATCCTGTT GACCAAGGCC CCCCTGAACC CCAAGGCCAA CCGTGAAAAG
#A._granosa_1/24 ... ... ... ... ... ...
#A._granosa_1/48 ... ... ... ... ... ...
#A._granosa_2/24 ... ... ... ... ... ...
#A._granosa_2/48 ... ... ... ... ... ...
#A._granosa_10/24 ... ... ... ... ... ...
#M._yessoensis ... .TG....CC. ...AG... ..A..T.... ... .A.G...
#H._cumingii ..A..A.... .TG.T... ...AG.A..T ...C.... .T..A... .A.A...
#A._granosa_0 ATGACCCAAA TCAT #A._granosa_1/24 ... .... #A._granosa_1/48 ... .... #A._granosa_2/24 ... .... #A._granosa_2/48 ... .... #A._granosa_10/24 ... .... #M._yessoensis ...A..G. .... #H._cumingii ...A..G. ....
Lampiran 2. Sekuen asam amino gen -aktin dari Anadara granosa dan bivalvia lainnya
#MEGA
!Title mega aktin bivalvia_AA; !Format
DataType=Protein NSeqs=8 NSites=124
Identical=. Missing=? Indel=-;
!Domain=Data;
#A._granosa_0 MSGEDDVAVF VVDKGFGMCK AGFAGDDAPR AVFPFIVGGP RHQSMMWGML QMDFYLGNEA
#A._granosa_1/24 ... ... ... ... ... ... #A._granosa_1/48 ... ... ... ... ... ... #A._granosa_2/24 ... ... ... ...R. ...V.... ... #A._granosa_2/48 ... ... ... ...R. ...V.... ... #A._granosa_10/24 ... ... ...S... .F...N..R. ... ... #M._yessoensis ...AL ...N.S.... ... ....S...R. ...GV.V..G .K.S.V.D.. #H._cumingii ...AL ...N.S.... ... ....S...R. ...GV.V..G .K.S.V.D..
#A._granosa_0 QRKKGILTLK YPMDNGIFTN WNNMKKIWHH TFYNELRVVP EEHPILLTKA PLNPKANREK #A._granosa_1/24 ... ... ... ... ... ...
#A._granosa_1/48 ... ... ... ... ... ...
#A._granosa_2/24 ... ... ..K... ... ... ...
#A._granosa_2/48 ... ... ..K... ... ... ...
#A._granosa_10/24 ... ... ..K... ... ... ...
#M._yessoensis .S.R... ..IEH..V.. .DD.E... ...A. ....V...E. ...
Lampiran 2. Lanjutan #A._granosa_0 MTQI #A._granosa_1/24 .... #A._granosa_1/48 .... #A._granosa_2/24 .... #A._granosa_2/48 .... #A._granosa_10/24 .... #M._yessoensis .... #H._cumingii ....
Lampiran 3. Sekuen nukleotida gen Hsp70 dari beberapa bivalvia
#MEGA
!Title mega hsp70 bivalvia_NT; !Format
DataType=Nucleotide NSeqs=10 NSites=527
Identical=. Missing=? Indel=-; !Domain=Data;
#C._gigas AAGCTGGACA AGGCCCAGAT CCACGACATC GTCCTGGTCG GAGGATCCAC ACGTATCCCA #C._virginica ..A..A.... .AT..A.... ...GG.G ..T...G. ....G..G.. GA.G..T..C #O._edulis ... ... ... ... ... ... #A._granosa ...A.... ... T..T..T..T ..A..T..T. .T..T..T.. CA.A..T... #T._granosa ....A... ...A.C.. T..T..T..T ..A..T..T. .T..T... CA.A..T... #M._galloprovincialis ..A..A.... ....TGCTG. ...T..A..T ...T....A. .T...A.. CA.A..T... #A._irradians ..A..C.... .AAGTTTA.. T..T...A ..A...T. .T..G... ...T... #P._fucata ...A... .A.GT..A.. T...T... ...T. ....C..T.. CA.A..T..C #C._farreri ..AA.T.... .AT..GCA.. ...T...T ..A..A..T. ....T... T...T... #P._penguin ...A... ...ACA.. ...T...T ...A..A. ....G..A.. ...T..T #C._gigas AAGATTCAGA AACTACTTCA GGACTTCTTC AACGGCAAGG AACTGAACAA ATCCATCAAC #C._virginica ..AG... .GA.G..G.. ...T...A.G GGT...A. ... G...T #O._edulis ... ... ... ... ... ... #A._granosa .GA... .GT.GT.G.. ...T... ..T..T..A. ... ... #T._granosa ..A... .GT.GT.G.. ...T... ..T..T..A. ... ... #M._galloprovincialis ..A..C.... .GT... ...T..T C.A...A. ..T... ...T... #A._irradians ...C.... ....T... A... ..T...A. ... ...T..T #P._fucata ..A..C..A. ....G..G.. A..T... ... ... ... #C._farreri ...C.... ....T... ... ..T...A. ... G...T..T #P._penguin ..A..C.... .G..C..A.. ...T... ..T...A. .G..C... G..G...
Lampiran 3. Lanjutan
#C._gigas CCTGACGAGG CTGTTGCTTA TGGAGCAGCT GTCCAGGCCG CCATTCTGTC CGGAGACAAA #C._virginica .AA... .C..G..C.. C..T..T..C ... ...AAA G...GG #O._edulis ... ... C... ... ... ... #A._granosa ..A..T..A. ....A..C.. ... ..T..A.GA. ...T.... T..T..TC.. #T._granosa ..A..T..A. ....A..C.. ... ..T..A.GA. ...T.... T..T..TC.. #M._galloprovincialis ...T..A. ....A..A.. C..T... ..G...A. ...T.... A..T...G #A._irradians ..C..T.... .C..C..C.. ...T... ...A..A. .T..C... ...G #P._fucata ...A. ....A..C.. ...T...G ..A...A. ...T.... T..C... #C._farreri ...T.... ...C.. ...T... ..G...A. ....C..T.. T...G #P._penguin ..A..T..A. ....A..G.. ...C... ..G...T. ....CT.... G... #C._gigas TCCGAGGAGG TACAGGACTT GCTCCTGTTG GACGTCACCC CCCTGTCCTT GGGTATTGAA #C._virginica AGT..C.TCA .CA.A...G. T...G.. ..T... .A...TC. ...C... #O._edulis ... ... ... ... ... ... #A._granosa ..T..A.... .G...TC. T...T... ..T..AG.A. .AT.A... ...G #T._granosa ..T..A.... .G...TC. T...T... ..T..AG.A. .AT.A... ...G #M._galloprovincialis ..A..A.... ....A... .T.AT....A ..T..T.... .A...TC. T... #A._irradians ..G... .C..A..TC. A..GT... ..T..AG.T. .AT...C. ...C..G #P._fucata ..A...A. .G...TC. C... ...TG... .G..T...C. C...C..G #C._farreri ..T... .C...TC. .T.GT... ..T..AG.T. .TT... ...G #P._penguin ... ...TC. .T.G...A ..T..GG... .A...G.. ...C..G #C._gigas ACAGCCGGAG GGGTGATGAC CAATCTCATC AAGAGAAACA CCACCATTCC AACCAAACAG #C._virginica ..T... .A..C... ...GA....T G.AC.C..TG ...AG... C...GGCA #O._edulis ... ... ... ... ... ... #A._granosa ..T...C. .T..A... A.CA..G..T ..AC.T.... .A..TG.C.. ...G..A #T._granosa ..T...T. .T..A... A.CA..G..A ..AC.T.... .A..TG.C.. ...A #M._galloprovincialis ...T.... .T... AGC...A... ..AC.T..T. .A... ...A... #A._irradians ..C..T.... .A... ATCC..T... ...C.T.... .A..TG.C.. C..A... #P._fucata ...C. .T... GTCG... ...G.... .G..A..C.. C...G... #C._farreri ..T..A.... .A... ATCA..T... ..AC.T.... .A..TG.C.. C...G... #P._penguin ..G..T.... .T... TTCA..G... ...C... .A... C...A
Lampiran 3. Lanjutan
#C._gigas ACGCAGACCT TCACCACATA CTCCGACAAC CAACCAGGTG TGTTGATTCA GGTGTATGAG #C._virginica T.C...G. ...C.. T... ..G..T..A. ...CC..C.. ...T.... #O._edulis ... ... ... ... ... ... #A._granosa .AC... ... T..T...T ..G... .AC.T..C.. ...C.T.... #T._granosa .AC... ... T..T...T ..G... .AC.C..C.. ...C.T.... #M._galloprovincialis ..A..A.... ....T..C.. ...T...T ..G..T.... .A..A..C.. ...T...A #A._irradians ..C..A..A. ...C.. ...T...T ..G..T.... ...C.. A..A..C... #P._fucata ..A... ...T.. ...T ...G.... .AC.C... ...A..C..A #C._farreri ..C..A..A. .T... ...T...T ..G..T.... ...C.. ...T... #P._penguin ..T..A..A. ...C.. ...G...T ...T..A. .AC... ...A..C... #C._gigas GGAGAGCGAG CCATGACCAA GGACAACAAC CTACTCGGAA AGTTCGAGCT GACTGGAATT #C._virginica ..G...A.G. .T... ... AA.T.G..C. CC..T..A.. ..AC... #O._edulis ... ... ... ... ... ... #A._granosa ...A..T. .A... A..T... ..G..T..T. .A..T..A.. ...T..C #T._granosa ...A..T. .A... A..T... ..C..T..T. .A..T..A.. ...T..C #M._galloprovincialis ...A... .T... ... T.G..T.... ....T..AT. A...A #A._irradians ...A.... .A... A...T ..G..T.... ....T..A.. ...C... #P._fucata ...A... ... A..T...T ...A.... ....T..A.. ...T..C #C._farreri ...A.... .A... A...T ..G... ....T..A.. ...A... #P._penguin ...AA.GA ... A... T.G..A.... ....T... ...T..C #C._gigas CCCCCCGCAC CCAGGGGTGT GCCCCAGATT GAGGTCACAT TTGACATTGA TGCCAACGGT #C._virginica ...G..T. ..C.T... C..G..A..C ..A..GGAG. .C...C.. C...C #O._edulis ... ... ... ... ... ... #A._granosa ..G..A.... .TG.T... CA.A....GC ..A..G...A .G....ACCG GTA.T.AT.G #T._granosa ..A..A.... .TC.T... TT.A... ..A..T..C. ....T... ...T..T... #M._galloprovincialis ..T..A.... .A..A... ... ..A..G..C. ... ...T... #A._irradians ..A..A.... ....A... T..A... ...T..C. .C..TG.... ... #P._fucata ...T..C. ..C.T... A..A... ... .C...C.. ...T... #C._farreri ..A..A.... .A..A... A..T..A... ...T.... ....TG.... ... #P._penguin ..A..T.... .TC.T... ...A... ...C. .C... ...T..T...
Lampiran 3. Lanjutan
#C._gigas ATCCTGAATG TGTCAGCTGT CGACAAGAGC ACAGGAAAGG AGAACAA
#C._virginica ...C. .T..G..CAA A... ...C...T CT...
#O._edulis ... ...C.. ... ... ...
#A._granosa TAT..TG.AT GTC..TGCCA TTGAC.AGAG CACA.G..A. G..GA.C #T._granosa ...T... ..CAT..CA. T...T ...A. ...
#M._galloprovincialis ... .A..T..A.. A..T...T ..T..T..A. ...
#A._irradians ...T... ....T..C.. ... ..T..C.... .A...
#P._fucata ...A....C. .TCAG..CAC G..T...G.. ..C..C.... .A...
#C._farreri ...C. .T..T... T...T ..C..C..A. ....T..
Lampiran 4. Sekuen asam amino gen Hsp70 dari beberapa bivalvia
#MEGA
!Title mega hsp70 bivalvia_AA; !Format
DataType=Protein NSeqs=10 NSites=175
Identical=. Missing=? Indel=-; !Domain=Data;
#C._gigas KLDKAQIHDI VLVGGSTRIP KIQKLLQDFF NGKELNKSIN PDEAVAYGAA VQAAILSGDK #C._virginica ....SK..EV ... .V..M....M G... Q... ...K..R #O._edulis ... ... ... ... ... ... #A._granosa ... ... R... ... ... ..G...Q #T._granosa .Q...N.... ... ... ... ... ..G...Q #M._galloprovincialis ...AV.E. ... ... Q... ... ... #A._irradians ....SL.... ... ... ... ... ... #P._fucata .M..G... ... ... ... ... ... #C._farreri .I..SA.... ... ... ... ... ... #P._penguin .M...T.... ... ... ... ... ...
#C._gigas SEEVQDLLLL DVTPLSLGIE TAGGVMTNLI KRNTTIPTKQ TQTFTTYSDN QPGVLIQVYE #C._virginica .DVIK.V..V ... ...KI. E..AK....A S... ....S...F. #O._edulis ... ... ... ... ... ... #A._granosa ... ..A... ...T.. ...V.... N... ...F. #T._granosa ... ..A... ...T.. ...V.... N... ...F. #M._galloprovincialis ... ... ...A.. ... ... ... #A._irradians ... ..A... ...S.. ...V.... ... ... #P._fucata ... ..A... ...S.. ... ... ... #C._farreri ... ..A... ...S.. ...V.... ... ... #P._penguin ... ..A... ...S.. ... ... ...
Lampiran 4. Lanjutan
#C._gigas GERAMTKDNN LLGKFELTGI PPAPRGVPQI EVTFDIDANG ILNVSAVDKS TGKEN #C._virginica ... K..T...N.. ... ..E... ...K... ...S.
#O._edulis ... ... ... ... ... ...
#A._granosa ... ... ....G..T.S ...M.NRY*W Y.ECPCH*QE HR.GR #T._granosa ... ... ...S.. ... ....H.I... ... #M._galloprovincialis ... ... ... ... ... ... #A._irradians ... ... ... ...V.... ... ... #P._fucata ... ... ... ... .M..Q.T..G ... #C._farreri ... ... ... ...V.... ... ... #P._penguin ...T... ... ... ... .M... ...
Lampiran 5. Persentase ketidakmiripan (p-distance) nukleotida sekuen gen Hsp70. 1. C.gigas, 2. C.virginica, 3. O.edulis, 4. P. fucata, 5. A.granosa, 6. T. granosa, 7. A.irradians, 8. C. farreri, 9. P. Penguin, 10. M. galloprovincialis
Takson 1 2 3 4 5 6 7 8 9 2 0.264 3 0.004 0.260 4 0.192 0.290 0.192 5 0.298 0.349 0.298 0.268 6 0.228 0.307 0.228 0.213 0.127 7 0.199 0.294 0.199 0.216 0.288 0.203 8 0.190 0.292 0.194 0.230 0.262 0.195 0.112 9 0.186 0.304 0.190 0.197 0.287 0.201 0.197 0.182 10 0.201 0.300 0.199 0.245 0.290 0.222 0.207 0.199 0.201
Lampiran 6. Persentase ketidakmiripan (p-distance) asam amino sekuen gen Hsp70. 1. C.gigas, 2. C.virginica, 3. O.edulis, 4. P. fucata, 5. A.granosa, 6. T. granosa, 7. A.irradians, 8. C. farreri, 9. P. Penguin, 10. M. galloprovincialis
Takson 1 2 3 4 5 6 7 8 9 2 0.185 3 0.000 0.185 4 0.046 0.214 0.046 5 0.162 0.306 0.162 0.173 6 0.069 0.214 0.069 0.081 0.133 7 0.035 0.197 0.035 0.052 0.162 0.069 8 0.040 0.202 0.040 0.052 0.168 0.069 0.012 9 0.035 0.208 0.035 0.035 0.179 0.075 0.040 0.040 10 0.029 0.185 0.029 0.069 0.185 0.087 0.052 0.052 0.052
Lampiran 7. Sebaran data panjang cangkang kerang darah Bojonegara, Panimbang, dan Kuala Tungkal 0 20 40 60 80 100 120 1.75 - 2.00 2.00 - 2.25 2.25 - 2.50 2.50 - 2.75 2.75 - 3.00 3.00 - 3.25 3.25 - 3.50 3.50 - 3.75 3.75 -4.00 > 4.00 F r e k u e n si Panjang cangkang (cm) Bojonegara Panimbang Kuala Tungkal
Lampiran 8. Hasil analisis diskriminan kanonik sepuluh karakter morfologi kerang darah darah dari Bojonegara, Panimbang, dan Kuala Tungkal
Karakter Can1 Can2 Can3 Can4 Can5 Can6 Can7 Can8 Can9 Can10
Panjang 0.390182* 0.660598* -0.067815 -0.064759 0.028928 0.093077 -0.202735 0.299813 -0.349059 -0.374491 Lebar 0.338511* 0.747581* -0.075081 -0.079398 0.049234 0.116046 -0.154851 0.342342 0.226407 -0.325982 Tebal 0.446204* 0.655531* -0.068248 -0.060763 0.019259 0.086228 0.369424 0.317680 -0.030464 -0.341617 Tinggi umbo 0.170058 0.111149 -0.013096 -0.004704 -0.011847 0.962540 0.031647 0.112726 -0.045779 -0.126906 Simetri kanan -0.554373 0.449089* -0.032871 -0.092636 0.150878 0.166965 0.197739 0.220055 -0.209639 -0.547004 Simetri kiri -0.065505 0.107497 0.985482* -0.009710 0.016911 -0.021323 0.053601 0.019506 -0.066279 -0.067059 Jumlah alur -0.140835 -0.149974 0.045855 0.011856 0.001546 0.058052 -0.048495 0.940631 -0.186511 -0.173589 Jumlah crenula -0.200140 0.697182* -0.083826 -0.104828 0.128681 0.030290 0.115238 0.230778 -0.229035 0.565149 Berat total 0.416595* 0.583615* -0.121890 -0.053468 0.529609 0.080673 -0.028902 0.288661 -0.072863 -0.302760 Berat tubuh 0.326130* 0.616877* -0.120238 0.287536 0.494488 0.045516 -0.000979 0.271522 -0.106722 -0.290281
Lampiran 9. Hasil analisis diskriminan Fisher linear sepuluh karakter morfologi kerang darah darah dari Bojonegara, Panimbang, dan Kuala Tungkal
Karakter morfologi Total
Simbaku Pooled Simbaku Antar Simbaku R-Square (1-RSq) F Value Pr > F Panjang 0.6119 0.4738 0.4751 0.4026 0.6738 195.41 <.0001 Lebar 0.4668 0.3696 0.3500 0.3754 0.6010 174.30 <.0001 Tebal 0.4447 0.3299 0.3657 0.4517 0.8238 238.90 <.0001 Tinggi umbo 0.1985 0.1897 0.0730 0.0903 0.0993 28.79 <.0001 Simetri kanan 0.4624 0.3209 0.4080 0.5200 1.0835 314.23 <.0001 Simetri kiri 2.1692 2.1529 0.3595 0.0183 0.0187 5.42 0.0047 Jumlah alur 1.9700 1.9044 0.6320 0.0687 0.0738 21.40 <.0001 Jumlah crenula 2.6176 2.2749 1.5928 0.2473 0.3285 95.27 <.0001 Berat total 4.8598 3.7291 3.8229 0.4132 0.7042 204.23 <.0001 Berat tubuh 1.1317 0.9252 0.8004 0.3340 0.5016 145.45 <.0001
SUMMARY
NURLISA ALIAS BUTET. Phenotypic Plasticity on Blood Cockle Anadara granosa L. as a Response to Environmental Pollution: a Case Study in Coastal Waters of Banten. Supervised by DEDY DURYADI SOLIHIN, KADARWAN SOEWARDI, and ASEP SAEFUDDIN.
Blood cockle Anadara granosa is a commercial bivalve inhabiting intertidal ecosystem. Coastal waters of Banten such Banten Bay, Bojonegara and Lada Bay, Panimbang are potential areas for blood cockle grow out. Banten Bay is a semi-closed waters facing North Coast of Java. Such industries as coal