HASIL DAN PEMBAHASAN
SIMPULAN DAN SARAN Simpulan
Simpulan
Tembakau transgenik lebih toleran terhadap cekaman Al dan pH rendah dibanding tembakau tipe liar. Hal ini mengindikasikan bahwa gen B11 merupakan gen yang berperan penting dalam mekanisme toleransi terhadap cekaman Al. Mekanisme toleransi terhadap cekaman Al pada tembakau transgenik diduga melalui mekanisme asam sitrat. Selain merupakan gen toleran Al, gen B11 juga diduga berperan dalam toleransi terhadap cekaman pH rendah serta berperan dalam proses pemanjangan sel akar dan pembentukan rambut akar.
Saran
Penelitian pendekatan secara molekuler perlu dilakukan untuk membuktikan bahwa gen B11 merupakan gen yang berperan dalam toleransi terhadap cekaman Al dan pH rendah, serta peran gen B11 dalam proses pemanjangan sel akar, pemanjangan akar dan rambut akar
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Lampiran 1 Komposisi Media Murashige-Skoog untuk Seleksi Biji Kode Senyawa Berat yang di Timbang (gram) Dilarutkan menjadi (ml) Konsentrasi stok (mg/L)
Volume yang diambil untuk membuat 1 L MS (ml) Final Konsentrasi (mg/L) A NH4NO3 82.5 1000 82500 20 1650 B KNO3 95 1000 95000 20 1900 C CaCl2.2H2O 22 250 88000 5 440 D H3BO3 0.31 250 1240 5 6.2 KH2PO4 8.5 34000 170 CoCl2.6H2O 0.0013 5.2 0.026 Na2MoO4.2H2O 0.0125 50 0.25 KI 0.0415 166 0.83 E MgSO4.7H2O 18.5 250 74000 5 370 MnSO4.4H2O 0.7527 3010.8 22.3 ZnSO4.7H2O 0.43 1720 8.6 CuSO4.7H2O 0.0013 5.2 0.025 F Na-EDTA 1.86 250 74.4 5 37.2 FeSO4.7H2O 1.39 5.56 27.8 Vit. B5 Myo-inositol 1 100 10000 10 100 Thiamine-HCl 0.1 1000 10 Nicotinic acid 0.01 100 1 Pyridoxine-HCl 0.01 100 1 Sukrosa 30 1000 Gellan gum 3 1000 26