LIST OF APPENDICES
3.15 Procedure of recording data .1 Growth parameters
3.15.1.1 Percent germination (seedling emergence)
Percent germination was measured by calculation seedling emergence after sowing. Daily observation was done for the first ten days in each plot and number of seedling emergence was counted. Percent germination was calculated by the following formula:
Total number of seedling emergence
Percent germination = --- × 100 Total seeds sown in the plot
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3.15.1.2 Plant height
Five plants were selected randomly from the inner rows of each plot. The height of the plants was measured from the ground level to the tip of the plant at 25, 50, 75 and 100 DAS. The mean value of plant height was recorded in cm.
3.15.1.3 Number of branches plant-1
The branches plant-1 was counted from five randomly sampled plants. It was done by counting total number of branches of all sampled plants then the average data were recorded.
3.15.1.4 Number of leaves plant-1
Five plants were selected randomly from the inner rows of each plot. Leaves plant-
1 was counted from each plant sample and then averaged at 25, 50, 75 and 100 DAS.
3.15.1.5 Leaf length
Leaf length was measured by using a meter scale. The measurement was taken from base of leaf to tip of the petiole. Average length of leaves was taken from five randomly selected plants sampled from inner rows of each plot. Data was recorded at 25, 50, 75 and 100 DAS. Mean data was expressed in centimeter (cm).
3.15.1.6 Leaf breadth
Leaf breadth was recorded as the average of leaves from randomly selected five plants of inner rows of each plot at 25, 50, 75 and 100 DAS. Thus mean was recorded and expressed in centimeter (cm).
3.15.1.7 Shoot length
Five plants were selected randomly from the inner rows of each plot. The shoot length of the plants was measured from the ground level to the top of the shoot at 30, 60, 90 and 120 DAS. The mean value of shoot length was recorded in cm.
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3.15.1.8 Root length
Root length was measured from randomly selected five plants from each plot at 30, 60, 90 and 120 DAS. The root length of the plants was measured from the ground level to end of the longest root. The mean value of root length was recorded in cm.
3.15.1.9 Number of nodules plant-1
Number of nodules plant-1 was measured from randomly selected five plants from each plot at 30 and 60 DAS. Nodule number was counted from five plants and mean value was recorded.
3.15.1.10 Dry weight plant-1
Five plants were collected randomly from each plot at 30, 60, 90 and 120 DAS.
Leaves, roots, and shoots were separated and put into envelop separately and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level. Total dry weight of separated parts were measured and the mean of dry matter weight plant-1 was determined in gram.
3.15.2 Dry matter partitioning 3.15.2.1 Leaf dry weight plant-1
From five randomly collected plants, leaves were separated and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level and the mean leaf dry weight plant-1 was determined in gram at 30, 60, 90 and 120 DAS.
3.15.2.2 Root dry weight plant-1
From five randomly collected plants, roots were separated and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a
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constant level and the mean root dry weight plant-1 was determined in gram at 30, 60, 90 and 120 DAS.
3.15.2.3 Shoot dry weight plant-1
From five randomly collected plants, shoots were separated and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level and the mean shoot dry weight plant-1 was determined in gram at 30, 60, 90 and 120 DAS.
3.15.2.4 Nodule dry weight plant-1
Nodules were separated from five randomly collected plants and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level and the mean nodules dry weight plant-1 was determined in gram at 30 and 60 DAS.
3.15.2.5 Pod dry weight plant-1
Pods were separated from five randomly collected plants and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level and the mean pod dry weight plant-1 was determined in gram at 90 and 120 DAS.
3.15.3 Yield contributing parameters 3.15.3.1 Fresh weight plant-1 at harvest
At the time of harvest, randomly selected whole five plants with pod was taken after removing soil and other stubbles from the plants and then mean weight was recorded and expressed in gram (g).
3.15.3.2 Number of pods plant-1
The pods plant-1 was counted from five randomly sampled plants. It was done by counting total number of pods of all sampled plants then the average data were recorded.
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3.15.3.3 Number of seeds pod-1
The seeds pod-1 was counted from 50 randomly sampled pods of each plot. It was done by counting total number of seeds of all sampled pods then it was calculated from total number of seeds divided by total number of pods.
3.15.3.4 Pod length
Pod length was recorded from randomly selected 20 pods of each plot and the average was taken and expressed in centimeter (cm).
3.15.3.5 Fresh weight of pods plant-1 at harvest
At the time of harvest, pods were separated from randomly collected five plants and fresh pod weight was taken and the mean pod fresh weight plant-1 was determined in gram.
3.15.3.6 Dry weight of pods plant-1 at harvest
At the time of harvest, from five randomly collected plants, pods were separated and put into envelop and placed in oven maintaining 70°C for 72 hours for oven dry until attained a constant level and the mean pod dry weight plant-1 was determined in gram.
3.15.3.7 Weight of 100-seed
From the seed stock of each plot 100 seeds were counted randomly and the weight was measured by an electrical balance. It was recorded in gram (g).
3.15.4 Yield parameters 3.15.4.1 Pod yield plot-1
Pod yield plot-1 was calculated from unshelled, cleaned and well dried grains collected from each plot and expressed as t ha-1 on 8 % moisture basis.
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3.15.4.2 Pod yield ha-1
Pod yield was calculated from unshelled, cleaned and well dried grains collected from the central 1 m2 area of inner rows of each plot (leaving boarder rows) and expressed as t ha-1 on 8 % moisture basis.
3.15.4.3 Stover yield
Stover yield was determined from the central 1 m2 area of inner rows of each plot.
After threshing, the sub sample was oven dried to a constant weight and finally converted to t ha-1.
3.15.4.4 Biological yield
It was the total yield including both the economic and stover yield as follows:
Biological yield = Grain yield + Stover yield 3.15.4.5 Harvest Index
Harvest index is the ratio of economic (grain) yield and biological yield. It was calculated by dividing the grain yield from the harvested area by the biological yield of the same area and multiplying by 100.
Grain yield
Harvest Index (%) = --- × 100 Biological yield