Contents
Sl. No. Chapter Subject Page No.
1 Acknowledgement i
2 Abstract ii
3 I Introduction 01-02
4 II Review of Literature 03-04
5 III Materials and Method 05-09
6 IV Results and discussion 10-16
7 V Conclusion 17
8 VI References 18-20
List of Tables
Sl.No. Title Page No.
1 Antimicrobial activity against ten pathogenic bacteria
10 2 Comparing the effect of M. indica and loperamadie on mean
defecation of albino rats using Student’s‘t’ test 12 Effect o3
ethanol Extract of M. indica leaves on castor oil induced diarrhoea. 13
4 Antidiarrhoeal effect of Mango leaves on castor oil induce
diarrhea 13
5
Brine shrimp lethality bioassay of ethanol extract of
Mango(Mangifera indica) leaf 15
6
Calculation of LC50 value, regression equation and
confidence limit by probit analysis. 16
List of Figures
Sl. No. Title Page No.
1 Shed dried leaves sample of Mangifera indica 08
2 Grinder 08
3 Extraction process of Mangifera indica 08
4 Alocoholic dipping of dried leaves (Mangifera indica) 08
5 Anti-microbial test Mangifera indica 09
6 Anti diarrhoeal test in rats. 09
7 Different concentration of brine shrimp larvae.
09
8 Anti microbial test on dried diffusion method 09
9 Zone of inhibition produced by Mangifera indica 11
10 Effect of ethanol extract of M. indica leaves 14
11 Regression Line and experimental point 15
Antimicrobial,Antidiarrhoel and cytotoxic effect of crude extract of Mango(Mangifera indica)leaves in
Bangladesh
A Clinical Report submitted as per approved style and content
………...…………
Supervisor
Dr. Shahneaz Ali khan Lecturer,
Department of Physiology, Biochemistry and Pharmacology
Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong
……….
Co-supervisor
Mohammad Mahabub uz-zaman Scientific officer
Dept.Drugs and Toxins Research division
BCSIR Laboratories Chittagong.
Chittagong-4220
……….
Author
Name: Md.Torikul Islam Roll No:2002/110 Reg. No: 142 Intern ID: I-44
Date of submission: 18 June’
Antimicrobial,Antidiarrhoel and cytotoxic effect of crude extract of Mango(Mangifera indica)leaves in
Bangladesh
Roll No: 2002/110 Reg. No: 142 Intern ID: I-44
Session: 2001 – 2002
Report presented in partial fulfillment for the Degree of Doctor of Veterinary Medicine (DVM)
Faculty of Veterinary Medicine
Chittagong Veterinary and Animal Sciences University
Khulshi, Chittagon-4202
June’ 2009
Acknowledgement
The author wishes to thanks beloved teacher DR.Shahneaz Ali Khan, Lecturer, Dept. of Physiology, Pharmacology and Biochemistry.for providing facilities to conduct this study.
The author would like to express his deep sense of gratitude and thanks to Mahbub-uz- zaman, Md. Saiful Islam, Nazim uddin Ahmed, Rasheda Akter, Scientific officer,BCSIR Laboratories,Chittagong.
The authors wishes to express his special thanks to all the friends specially Md Abdul alim (Sobur) for his cooperation during the preparation of this report.
The author
i
Title:
Antimirobial, Antidiarrhoeal and Cytotoxic effect of crude extract from Mango (Mangifera indica) leaves in Bangladesh.
Abstract
Preliminary phytochemical screening of the ethanolic extract of the leaves of the Mangifera indica (Family- Anacardiaceae) revealed the presence of alkaloids, flavonoids, reducing suger, tannins and saponins. The antidiarrhoeal activity of the extract (200 mg/kg) was assessed on experimental animal and a dose dependent decrease in the total number of fecal droppings was observed in castrol oil induced diarrhea in rat. The antimicrobial effect of the ethanolic extract of the mango leaves was studied according to the diffusion method. The extract showed an activity with regard to eight bacteria species- Bacillus subtilis (BTCC 17), B. megaterium (BTCC 18) B. cereus (BTCC 19), Staphylococcus aureus (ATCC6538),Proteus sp[CRL(ICDDR’ B)], Shigella sonnei [ CRL (ICDDR, B)] , Shigella flexinary[CRL(ICDDR’ B)] and Bacillus polimyxa(BTCC 16) .In cytotoxic determination, LC50 of the plant extract was 879.70µg/ml that indicate the wide range of LC50 value it means no cytotoxic effect was found.
Key words: Mangifera indica, antimicrobial activity, antidiarrhoeal effect, Cytotoxic effect.
ii
