Pewarisan sifat ketahanan cabai merah terhadap cucumber mosaic virus (CMV) dan analisis keterpautan marka RAPD dengan gen ketahanan terhadap CMV

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Inheritance of Cucumber mosaic virus (CMV)-Resistance 2 % 9 v q

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between

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Markers and CMV-Resistance Gene(s). Supemsing committee :

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SUDARSONO (chairman), RUSMILAH SUSENO and HAJRIAL

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CMV

resistance and its

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inheritance is important for breeding CMV resistance in hot pepper.

CMV

is the a v r ' ? X

3 n 0 most widwspread virus infecting hot pepper in Indonesia, and its infection has

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caused major yield reduction. Cumntly, such information is limitted, especially

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for commercially grown hot pepper in Iqdonesia. Therefore, fbrther studies on

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X these subject are necessary, The allelism study can be detected through crossing

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'CI among CMV-resistance genotypes, and this information is related to breeding

s P S Q UI strategy to develop high-yielding and CMV-resistance hot pepper cultivars. The

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use of hybrid genotype is one of the alternatives in increasing the national

2 8 g production of hot pepper. However, the limited availability of local hybrid has

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caused our pepper producers rely on and suffer from the high price of imported

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seeds. For this reason, the development of local hybrid of hot pepper was done.

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Molecular marker such as RAPD (Random Amplified Polymorphic DNA) has

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been used to tag certain desirable gene(s) in the plant genome by genetic linkage

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(LI This research consisted of four experiments; (1) Inheritance of Cucumber

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mosaic virus (CMV) resistance characters in hot pepper. This experiment aimed

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r to study the action of gene(s) controlling the CMV-resistance in hot pepper, to

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w estimate the number of resistance genes, and to estimate the heritability values.

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(2) The allelism among genes associated with CMV resistance in three CMV-

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Combining ability and heterosis in developing hybrids of hot pepper. This

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research aimed to investigate the growth and productivity, general and specific

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combining ability (GCA and SCA), and heterosis in order to develop the high-

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RAPD markers and CMV-resistance gene(s) in hot pepper using bulk segregant

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analysis, that aimed to develop genetic linkage analysis and identi@ specific

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RAPD marker linked to CMV resistance in hot pepper.

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7 The experiments used nine sets of crossing between three CMV-resistance

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(PBC 375, PBC 398 and PBC 535) and three CMV-susceptible genotypes (PBC

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378, PBC 1354 and Rawit). The analysis was done using Mendelian genetic test,

3 I. by comparing the ratio of phenotypic frequency on segregated F2 population

t! 0 against Mendelian ratio through Chi-square test, The identification of

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polymorphic RAPD marker for

CMV

resistance using Bulk Segregant Analysis

(BSA) was conducted by amplifying DNA in resistance and susceptible pools using a number of random primers. Resistance and susceptible DNA pools w e n

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_{obtained by mapping the segregated population (F2) resulted from crossing}

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between resistance parent "PBC 375" and susceptible parent "Rawit".