Phosphate Ion Level in Artificial Saliva After Aplication of CPP-ACP (Casein
Phosphopeptides-Amorphous Calcium)
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Abstrak
White spot lesion is a clinical sign of enamel demineralization. Demineralization is not balanced with remineralization, cause mineral content change of hydroxiapatit. To prevent, anticariogenic agent is applied to the teeth. It works by helping the process of tooth remineralization is Casein phosphopeptides Amorphous Calcium Phosphate (CPP-ACP). CPP can bind and stabilize calcium and phosphate ions in the solution, then bind in plaques and diffuse in enamel to replace hydroxyapatite structure. This study was aimed to analyze the level of phosphate ion in artificial saliva after CPP-ACP application on days 1, 3 and 7. This research used 4 treatment groups, group A (pH 7,00, B (pH 7,00 +CPP-ACP), C (pH 4,75) and D (pH 4,75+CPP-ACP). Samples 16 pieces of P-1 maxillary were immersed to artificial saliva for 7 days in a 37NC incubator. On days 1, 3 and 7 the levels of phosphate ion were measured by Spectrophotometer UV/Vis. Results were analyzed by Kormogorov-Smirnov, Levene then Kruskal-Wallis and Mann-Whitney. It showed there was a significant difference in each treatment group, with value of 0,000 (p<0.05). Except on day 3 that showed no significant difference, with value 0.149 (p>0.05), between group B and group D. The groups with the application of CPP-ACP had higher phosphate levels than the groups without application of CPP-ACP and the level of phosphate ion increased on day-1, 3 and 7. It concluded there were increase level of phosphate ion in artificial saliva on the teeth with CPP-ACP application. Keywords: CPP-ACP, demineralization, phosphate ion, remineralization.
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