Centella asiatica L. Urban Extract’s Immunomodulator Effect over the IgG of Male Mice Induced by BCG Vaccine

Centella asiatica

L. Urban Extract’s

Immunomodulator Effect over the

IgG of Male Mice Induced by BCG

Vaccine

Presented by :

To prove the immunomodulation

effect of

Centella asiatica

L. Urban

over the IgG of male mice induced

by BCG Vaccine

Investigational Product

 BCG Vaccine : bacille Calmette-Guerin, is a vaccine

for tuberculosis (TB) disease  All Group

Dosage : 6,5 µl/20g BW

 Aquadest : Placebo  Group I

 Levamisole : this medication stimulates the body's

immune system to help treat and protect against illness (Control +)

Dosage : 18,2 mg levamisol  Group II

 Prednisone : corticosteroid used for suppressing

Investigational Product



Centella asiatica extract (CAE) :

Kingdom Plantae

Subkingdom Viridiplantae

Infrakingdom Streptophyta

Superdivision Embryophyta

PROCEDURES

The study used 50% ethanol to extract the whole part of CA by maceration

Extract evaporation into its viscous form.

The viscous extract was divided into some dosages to be administered to the mice : 50mg_/

Kg BW, 100mg/Kg BW, 150mg/Kg BW, and

200mg_/

PROCEDURES

The dosages were administered orally in daily measure.

The blood samples were taken from the mice on day 0, 14, 28, 32 starting from the day of investigational

product administration.

CAE administration procedure

BCG vaccination procedure

Conclusions

The study result proved the

immunostimulant effect of Centella asiatica

extracts on dosage group 50

mg

_/

Kg

BW and

100

mg

_/

Kg

BW after each BCG vaccine