Centella asiatica
L. Urban Extract’s
Immunomodulator Effect over the
IgG of Male Mice Induced by BCG
Vaccine
Presented by :
To prove the immunomodulation
effect of
Centella asiatica
L. Urban
over the IgG of male mice induced
by BCG Vaccine
Investigational Product
BCG Vaccine : bacille Calmette-Guerin, is a vaccine
for tuberculosis (TB) disease All Group
Dosage : 6,5 µl/20g BW
Aquadest : Placebo Group I
Levamisole : this medication stimulates the body's
immune system to help treat and protect against illness (Control +)
Dosage : 18,2 mg levamisol Group II
Prednisone : corticosteroid used for suppressing
Investigational Product
Centella asiatica extract (CAE) :
Kingdom Plantae
Subkingdom Viridiplantae
Infrakingdom Streptophyta
Superdivision Embryophyta
PROCEDURES
The study used 50% ethanol to extract the whole part of CA by maceration
Extract evaporation into its viscous form.
The viscous extract was divided into some dosages to be administered to the mice : 50mg/
Kg BW, 100mg/Kg BW, 150mg/Kg BW, and
200mg/
PROCEDURES
The dosages were administered orally in daily measure.
The blood samples were taken from the mice on day 0, 14, 28, 32 starting from the day of investigational
product administration.
CAE administration procedure
BCG vaccination procedure
Conclusions
The study result proved the
immunostimulant effect of Centella asiatica
extracts on dosage group 50
mg/
Kg
BW and
100
mg/
Kg
