MICROBIOLOGY INDONESIA, August 2007, p 55-60
Volume 1, Number 2
ISSN 1978-3477
Evidence for a Link Between Pathogenicity and the Role of Imp
Bacterial Transport Effector Proteins in Soybean Infection by
Xanthomonas axonopodis
pv.
glycines
ANY FITRIANI
1, ANTONIUS SUWANTO
1, ARIS TRI WAHYUDI
1,
AND
BUDI TJAHJONO
21Department of Biology, 2Department of Plant Protection,
Institut Pertanian Bogor, Darmaga Campus, Bogor 16680
Xanthomonas axonopodis pv. glycines (Xag) is the c ausal age nt at bacte ria l pus tule dise ase of s oybeans. A non-pathoge nic mutant of Xag (M 715) was c ons tr ucte d e mploying tr ans pos on mutage nes is whic h s howe d similar epiphytic s urvival in planta to its wild type str ain (YR32). T he objective of this wor k was to ide ntify and to analyze ge nes involve d in pathoge nicity in Xag YR32. I nverse Polymerase Chain Reac tion (IPCR) was use d to is olate the DNA flanking transpos on inser tion. A 1.3 kb flanking DNA fragme nt was seque nced and analyze d e mploying BLAST pr ogr am to study homology, the position of transpos on inser tion and to pre dict the str ucture and f unc tion of the ge ne. One of the Ope n Reading Frames (ORFs) s hare d homology with inner membrane proteins (imps) of Xanthomonas axonopodis pv. citri
(GenBank accession No. NC 003919). Nor the r n blot analys is reve ale d that an imps ge ne was monocis tr onic and the size of imps mRNA in YR32 was slightly longer than in M 715. Reverse Transc riptase -PCR analysis demons trated that the imps transcr ipt in M 715 was muc h less abundant than I n the wild type YR32. Trans pos on (mini T n5Km'T p') was de ter mined to be ins erte d clos e to the e nd of C -terminal r egion of Imps ge ne and might be s uffic ie nt to destabili ze the imps transcr ipt I n M 715 and s o inf lue nce effectors trans por tation from Xag to plant ce ll.
