Abstract
In this study, the anatomy responses of Sorghum bicolor to chromium were investigated. To determine the responses arising for genotypic differences, Badik and UPCA varieties were used. Random Factorial Design was used in this study with three factors : chromium species (Cr (III) and Cr (VI)), concentration (0; 1,8; 4,5; and 13,6 mgCr kg-1 sand (w/w)), and Sorghum bicolor varities (Badik and UPCA). The results revealed that both Cr (III) dan Cr (VI) decreased number and size of vascular bundle, epidermis and mesophyll thickness, size and percentage stomatal but increased lignin thickness. The effect was more pronounced on Cr (VI) than Cr (III) caused its toxicity. The highest anatomy difference percentage was in number of vascular bundle that showed 52,8% difference in Cr(VI) 4,5 mgCr/kg for Badik and 46% in Cr(III) 13,6 mgCr/kg for UPCA in roots. In stems, the highest anatomy difference was in lignin thickness for Badik that showed 54% increased in Cr(VI) 1,8 mgCr/kg and number of vascular bundle for UPCA that showed 57,9% decreased in Cr(VI) 4,5 mgCr/kg. Meanwhile in leaves, the highest anatomy difference percentage was in number of vascular bundle that showed 81,4% decreased for Badik and 65,8% decreased for UPCA in Cr(VI) 4,5 mgCr/kg. In roots and stems, Badik had lower anatomy difference percentage than UPCA except for lignin thickness. In leaves, UPCA showed anatomy difference percentage lower than Badik.