Antiinflamatory Activity of Methanolic Extract of Mangifera Casturi in Thioglycollate-induced Leukocyte Migration on Mice

(1)

! ()) !

!"# $%&'_{& ( # !)#*+, -# !- #}._{& !-+/+}._{& !0} _{!) 1} _{2!+ +}%&' %_{* )" + , -.} _{)+ /0#& '# ' 1} _{2 .} _{3 4+ 5} ₀ _{0 '#} .₆ ₀ _{, -.} _{)+ * )" + ,} _. _{#) 0} _{)# 0) /0#& '# ' 7} _"04 _045" _{8 #} _{0 0} ₀ 0 0 '# 9 0 , " (0 # #0, ) #& % ' ). * )" + , -. )+# /0#& '# ' 1 2 . 3 4+ 5 0 0 '# ! " # # $ ! " # % $ # ! $ % & ' ! ( () % ! % # % ( ' ! ' ! " * + % ( ' % ! , # # # ( $ ( ( ' ( ! - % $ $ # % % $ ! . % / ( $ % $ ( ! 01 # ! % 2 ' ' ' ! 3 ' ' ' ' $ ' ' ( ( ( ' ' ' ( ( ! 4 ( 5 ' 5 ' $ ' ' ( ' 5 ' ' ' ! ' ' ' ' '' 5 ' ( ' ' ' ' ( ' ! ' ( ' * + 5 ! ! ' ' ' ' ( ' ! ! ( ' ( ( ' ! ( ' 5 ' ! ! ! 4 5 ' ' ' ' ( ' ' ! ' ' ' ' ' 6 +7 +7 + ) 33 ( & *87 87 6+ *87 9 : 87 : 8! " ' ' 5 ; - % ' ' 9+8 5 '' ( % ' ' ' ( ' + + ) 33 ( ' ' ' ! + ()3+ "# !- + 4 !"# / #* 4 56! /7 87#"

(2)

"' $ ' ' ' ( ' ( ( ( ' ( ' ( ' ! B5 0 0 5 '' ' ' ' ( ' % '! ' 2 ' 0, '# " 5 0 5'# 5 5' '#' # "0 0 0' '#,#5 + 04 # 5 0 5" " '# 0 5 #& '# "5 '# ' #0 ' # #0, 5'# 0, '# #' 5 0 . 2 0 0 : #)" #0, '# " "0 5 "' 5 0 ' 2 #04 0 '5# "0 #0, '# "2" 0 5 #, 04 0 #0, 5'# 0 )" " ". 0 2 #04 0 0 "0 2#5 #. 0 0 # 5 5 0 0# " 5 0 5 "' 5 0 0 04 5# 5 0 0+ 5# ; <# 2 2 0 % 044 0#' > $ # %= ' # 0# 0 '" 5'# = #5 .# 5'# => .# 4 0 5'# > = # #5# 0 0 #04 # $ + 04 #)" 0+ 5 0+ 5# ' # 5 05 0 # #0, '# 5 # & '5" # ' 0+ 5# 4 0 #& " "0 #'5 #5 %= 04#0#'# '# 5'# 5'# #, + 04 . + ' # 5'# '# # # 04. ' # '# # # 5 0 # #0 5 #& '# & & 04. ''#" ( - ' 0 #0 5 #& '# 5 0 ' #" 0 0" " 0 # # 0 + 04 # 5"5 0 % &# # ' 0+ < , 0 #5 , & 0 # 0 4 ?0 4 0 # #5# ,"04'# ' 4 # 0 # 5'# 0 # " ". % &# # 2 0 04 0 0 # # 0 ' " #044#0+ # $ 5 0 5" 040+ 0 # 5'# 0 " ". . ' "' #0, '# " " '"5 ' )# - #0, '# + 04 #' 5 0 . ) 2 #04 0 0 ' + 04 ) #)" 0 "5 0 ' 5# 0 + 04 ' 02" 0+ #" . 02 # # #0, '# # #0, '# 5 0 #)" 2 #0+ & ' # '# " ". ' .#044 & " . 0#045 # " ". ' " 0 0 0 04 5# 5 0 . 0 - # # ' " ". + 04 0#045 0+ 5 0 ' . " . < # #0 #04 5 # 0 2 # . ; 2 5 " # 0 "5 '# # 0 . 0 ' 0 0 #4 '# 0 "' #0 #04 " ". 0 '"5 5 044 2 #04 0 -5 0 #'# -5 0 " ". 0 . 0+ ' 4# 0 5 )# 5" < # #0 #04 " ". . "0 # 2 # #0, '# ' 0 5 #& '# ' .#044 ' 5#0 0+ 5 5" '"5 "5 '# + 04 < #0+ - ' ' #0, '# # " . # #0, '# " "0 # 0 ##0, '# # #0, '# 0+ 5 0 #0, '# "' 04'"04 # 0 0+ 7 @ * A 0 * B C #04 55 $ 7 7 7 * @ ; <# 2 2 0 % 044 0#' # 5#0 #0# 0+ 5 0 0#045 0+ 5' '# ' 5 #0 C 0 - "5 0 ' 0 + 04 0 0 #0+ 5 0 #5 0 04 0 #0 4 #0 "5 0 0 " ,# 8 5 0 2"4 04 ".# 5 " 0 "5 '# 5 2 #04 0 #0, '# ' # 7 % &C 0 & 9- C #04 55 $ * 5; 04 0 5' '# 4 0 + 04 045 '# 5#0 #0, '# 5 5#0 7 - A 9- D#0 5" . '# 9( 9( ' 5 #0 C 04#0 "5'# 0:# 9=E 0 # = ' , 5 " ". 0 9 #D 55 - ' ' 0+ ". 0 #0, '# # 5"5 0 04 0 0" "05 0 ' 0 5" #0, '# 0#045 5 0 ' 0 5" 0 ##0, '# 04. 0 # 0 5 5 0 '#' 0 % ' 0 #0, '# + 04 # 5 . 0 # #' 5 0 . # 5 ' # 040+ 5 #&# ' # #5 - ' 0 #0.# # ' "5 '# # #5# 5 #&# ' 0 ##0, '# - #0, '# 5 0#' ,"04'# ' "5 '# # 5 5 0 ' .#044 #0, '# # 5 . 0 # C #04 55 $ "2" 0 # 0 0 ##0, '# . 0) 4 . 5 040+ ' ' #0, '# 02 # #0, '# 5 0#' 0 4 ' ' #0, '# 5 '#' # 5 ' 5#0 . + 04 04 5# 5 0 .# 040+ ,"04'# 2 #04 0 0, '# + 04 # " ".5 0 . " ". . + 04 ) 04.# 045 0 , 5 #)" 0) 4 . #0, 5'# ) 04 #5 0 ,"04'# 2 #04 0 ' # ' " 0 # 5 #. 0F . 0 # ' . 04' 04 0 # " .# 04 ; <# 2 2 0 % 044 0#' + 04 2"4 ' # 4 0"' 04#, # "4 # #5# , 5 0 # 5'# 0 + 04 # # #5# 5 0 ' # ' " 4 0"' /2# 0 ." " 0 0 ,# 5# # + 04 # 5"5 0 "' #5 ' # 0 ( #+ 0# $ 0+ 5 0 .< 04 . 0 044 5 ' " # 04 0 "04 ' 0+ < 0 # ' # 0 ' 0#0 0" " ". 0 5' 5 # " . 044 5 ' " # 04 0 "04

(3)

, & 0 # #. 0+ 5 # "0 5 5 # 04 4 "044 04 " "0 5 ' 5 " # " 5 0 .< 5 5 0 #5 " . 044 5 ' " # 04 0 "04 ' 0+ < 4 04 0 0 # F' # 0 , 0 #5 - ' ' #0, '# 5 , 4 '5 0 #5 ' + 04 ,"04'# "' 5 0 '#04 #)" #0, '# 4 #' ' 4 # " 04 # #5 ' #0# 2"4 . + 4# 2 #04 0 " ". 0 02 # , 5 + 04 2 #0+ #0, '# ( # #5 ' + 04 2 # ' ' #0, '# #5" 04# 5 #' 02 # 0 #, 04 0 0 ##0, '# 044 5 ' " # + 04 . #5 ."# # #5# 5 #&# ' 0 # 5'# 0 + 04 #044# # "4 " 0 5 0 ' ' #0, '# ; ' 5 0 5 04 ' " 5 # " "'5 0 " "' 0 ' . 0 0 #0 ( 5 . 5' 5 0 #5 " . 044 5 ' " # "0+ # 5 #&# ' 0 ##0, '# "# 0 5 0 04. 0 #4 '# "5 '# G 0+ < + 04 # 5' 5 0 #5 " . 044 5 ' " #G - 0 # # 0 #0# "2" 0 "0 "5 04"2# 5 #&# ' 0 ##0, '# 5' 5 0 #5 " . 044 5 ' " # 0 04# 0 #,#5 '# 5 0 "04 0 ' 0+ < 0+ - 0 # # 0 #0# 02 # 0 ' 0 "0 "5 0 # # 0 0 # # 0 ' 02" 0+ 045 0) # . 0 "0 "5 0 ##0, '# ' 0 "5"04 04 04 0 0'# '" + 0 0 '# "0 "5 04 0 ( (*#-# ; . 0 . 0 + 04 #4"0 5 0 0 # # 0 #0# 0 #0 4 3 43 .# 4 #5 C )5 #0 '#0 #4 9 C )5 H" ' ' # # 0 ( D , C )5 0 50#' 5 , C )5 ( " #0#" , # " . 044 5 ' " # '# #) 1 * C )5 0 . 5' 0 C )5 # ' C )5 0 C )5 9 = 0#' .# ' '" , 0 * 9 > < 0 "2# + 04 #4"0 5 0 . 0)# 2 0 0 4 " ; F9 " " #044" + 04 # . # 7 #" * 5 4# * 5" ' * '# /1 (-+"( ( (*#-# (3 )# )#/3*#)# 0! / )-! # ; . 0 " + 04 #4"0 5 0 0 # # 0 #0# . " . 5 ' " # 8 0' + 04 . ' 5 ;" . # 0 0 # 8 " 0 " - &#0'# 8 # 0 0 0 ;" . 5 ' " # # # " 0 & 6 ' ;" . + 04 5 0 # # # # 5"5 0 #0 '# "0 "5 ' #5 0 5 0 0 2 0#' " ". 0 ' " 6 #0 '# " ". 0 # 5"5 0 # 7 #" ;# 4# * '# * 5" ' * '# /0#& '# ' 1 2 . 3 4+ 5 6 4#04 " . 5 ' " # ' 5" # " .0+ ## #' "0 "5 # #' .5 0 # #2#0+ ' 02" 0+ # 04 04 5 )# 0 #5 #045 0 '"." 9 ' . # # & 0 # #'# 5 #04 " #' "55 0 04 0 044"0 5 0 '#0 0+ "5 "0 "5 ' 02" 0+ # 5' 5'# " )- )# 0! / )-! # C5' 5 0 #5 # " 04 0 ' '# ' "5 5 #04 " . 0 5" # 044"0 5 0 0 ' 0+ 5 D '#04 '#04 ' 2 C5' 5 # 5 5 0 04 0 $ '"." 9 .#044 # 5 0 5' 5 5 0 C5' 5 5 0 # #0 .5 0 ) < 0 ' #0 0 '#' 0 #" 5 0 04 0 '"." 5 0 .#044 " #0+ 5 0 . #' 0+ 5 54 ' "5 5 #04 04. '# 5 0 $! 4 5' 5 0 0 5' 5'# ' ' $ ! I 0+ 5 0)# # 4# 02 # $ 5 5 5" 0 + 04 # # # 5 5 0 0 5" 0 5 5 5 0 " 6 = I 5 5 .# 4 #5 5 5 5 0 '# #, #0 '#0 5 5 5' 5 '#' 5 5 5' 5 '#' 0 5 5 5' 5 '#' 6 '#' + 04 #4"0 5 0 " " " " . J J 0 4 F54 ;; 9# -#:#- ) -## 5* / )# /2# 0 ##0, '# + 04 #4"0 5 0 + # " #4 '# "5 '# 0)# + 04 ##0 "5'# .# 4 #5 + 04 # #,#5 '# 4 - 5 # 0)# ##02 5'# 04 0 5' 5 " " "0 #0 '#0 ' 0+ 5 7 ' ) # ##5" # # 0 7 .# 4 #5 I ' ) # + 04 # #5 0 0# ' . # 0 5' 5 " " "0 #0 '#0 0)# #5 05 0 2 5 " # 0 04 0 044"0 5 0 5 , 8" # " # "5 5 " # 0 #'"0 #55 0 7 " 043 #04#0 ' ) . 0 044"0 5 0 ' "# #02 5'# - " 0)# #4 + 04 4 + 04 04 0 04 0 ' 0# 0 -; # # 5 # 044"0 5 0 ' "#

(4)

"' 0'# ' # 044 " + 04 # . # 0 5 0 04 0 ' 0 #,"4 0# K9 .#044 0 "5 ' " 0 0 # " 04 0 0+ # '"' 0'#5 0 5 # 04 0 -; 7 #< 0 # 04 0 # 0 ( 0 2" . ' "5 '# #.# "04 044"0 5 0 04 0 04 0 # < . #5 '5 *#)#) "! #/# ( )- "( -( # 6 5'# 5 0 "04 0 # ' 5"0 5' 5 0 #5 " . 044 5 ' " # 04 0 87 044"0 5 0 , ' 4 5 # ' 0 . 5' 0 ' # 50+ #5 0 ) 5 04 0 < 0 '#04 '#04 0+ "04" . " 0 ) 5 5'# 04 0 0#' .# ' '" , 1 " #' 04 0 0#' .# ' '" , ) 5 # 5 5'# # 04 0 '#0 5 "0 ' . #' 04 0 0#' .# ' '" , "0)" < 0 "04" + 04 0 0 5 0 0+ ' + < 4 04 0 # 0 6 5'# 87 04 0 , ' 4 5 # ' 0 . 5' 0 0 5'# ' ' #" '" , #5 0 ' # 50+ ) 5 < 0 ) 5 + 04 ' " #' # 5 5'# 04 0 # '#0 5 1 > #0# ' 5 # 4# 0"02"55 0 0+ ' 0+ < 4 04 0 0 # " 0+ 5 ) 5 #' 5# > + 04 5'# '# #, < 0 # " 1 > '# 0 #'#' 87 # 5' 5 0 #5 " . 044 5 ' " # 04 0 , ' # '# #5 4 * , ' 4 5 # ' 0 . 5' 0 0 5'# ' 0#' .# ' '" , - 04 0 '#0 5J / 0 J ) / 0 J '#0 5 ' . #' 0#' .# ' '" , 0 # 0 '5 0 1 > '# 0 #'#' 87 # 5' 5 0 #5 " . 044 5 ' " # 04 0 , ' # '# #5 4 * , ' 4 5 # ' 0 . 5' 0 0 5'# ' ' #" '" , - 04 0 '#0 5J / 0 J ) / 0 J '#0 5 ' . #' ' #" '" , 0 # 0 '5 0

(5)

4 . 5'# * 9 + 04 0"02"55 0 0+ ' 0+ < , 0 #5 5 #5 0 #'#' 87 # 5"5 0 04 0 044"0 5 0 , ' 4 5 + 04 #. 0 0 + # " 5 , 0 1 L " "0 ) 5 ' " #. # #04 0 "0 #' #. .< ) 5 ' " 04 . 5 5 0 "04 0 ' 0+ < , 0 #5 ; ' 5 0 "2# 87 + 04 # 5"5 0 5' 5 0 #5 " . 5 ' " # #04 # 5 04 0 "04 ' 0+ < # 0 0 , 0 #5 ; ' 5 0 # 0 #,#5 '# ' "5 " 0 # # 0 02" 0 ' 0+ < # 0 + 04 5 0 "04 . " 0 '# ' " ' 045 0 ' 0+ < , 0 #50+ . # 4 " 9# -#:#- ) -## 5* / )# /2# 5 #&# ' 0 ##0, '# # 5"5 0 044"0 5 0 #4 '# "5 '# 0)# + 04 ##0 "5'# .# 4 #5 > '# "2# # # 5 0 1 " . ' "5 '# 5 5 " # 5 '#40#,#5 0 04 0 5 5 0 ' .#044 " + 04 #4"0 5 0 # 5 0+ 5 0 #4 '# "5 '# + 04 '#40#,#5 0 0 "5'# #4 '# ' "5 '# 04 0 .# 4 #5 " 04#0 "5'# 2" . ' "5 '# 02 # 5 # #. #044# # 0 #045 0 5 5 0 > #0# 0"02"55 0 2 #0+ #0, '# + 04 # 0 # 04 0 #4 '# "5 '# 5# #0 "5'# .# 4 #5 - # 0 #0 '#0 0 ##0, '# " 0" "05 0 ' ) 1 > '# "2# 5 #&# ' 0 ##0, '# # 5' 5 0 #5 " . 044 5 ' " # 0)# + 04 ##0 "5'# .# 4 #5 0 '#0 $ 4F54 ;; 5' 5 0 #5 " . 044 5 ' " # '#' 0 " " " " J J 0 4 F54 ;; 0 M 0"02"55 0 '#40#,#5 0 # 0 #045 0 04 0 5 5 " 0 N 0"02"55 0 '#40#,#5 0 # 0 #045 0 04 0 5 5 .# 4 #5 1 > '# 04 0 87 # 5' 5 0 #5 " . 044 5 ' " # 04 0 , ' # '# #5 4 * , ' 4 5 5 , 0 0 5'# ' * 9 - 04 0 '#0 5J / 0 J ) / 0 J '#0 5 ' . #' * 9

(6)

'#40#,#5 0 2" . ' "5 '# 02 # ' 04 0 5 5 0 0 5 5 " > '# #0# 0"02"55 0 .< "2# 0 ##0, '# #0# 2 0 04 0 #5 5 0 #0 '#0 + 04 ,"04'# ' 4 # 5 0 '# #, " 0" "05 0 #4 '# "5 '# ' ) '#40#,#5 0 - # 0 5' 5 0 #5 " . 044 5 ' " # '#' " " 0" "05 0 2" . ' "5 '# ' ) '#40#,#5 0 "0 # 0 '#' 0 " 0" "05 0 2" . ' "5 '# - # 0 5' 5 0 #5 " . 044 5 ' " # '#' + 04 #04 ' '#' " 0" "05 0 2" . ' "5 '# ' 04 0 #0 '#0 5 5 0 0 5 5 " 6 04 0 #5# 0 5' 5 0 #5 " . 5 ' " # '#' 0 "0+ # 5 #&# ' 0 ##0, '# "# 04. 0 #4 '# "5 '# 0)# + 04 ##0 "5'# .# 4 #5 C5' 5 #0# 0'# "0 "5 #5 045 04 #. 02" ' 4 # '" ' 0+ < 0 ##0, '# + 04 0'# C5' 5 0 #5 " . 044 5 ' " # '#' 4 F54;; 0 '#' 4 F54;; # #5# 5 #&# ' 0 ##0, '# "# 04. 0 #4 '# "5 '# 0)# + 04 ##0 "5'# .# 4 #5 C5' 5 0 #5 " . 044 5 ' " # 04 0 "04 ' 0+ < 0 # 0 , 0 #5 8 # 04") 5 0 # 5 '#. 5 6 8 + 04 . # + # 0 # # 0 #0# "# 7-- /1 04 0 '5 .# . 5 '# ' 0 . '#'< 04 0 0 5 0 5 7--/1 F F7 F 044 "0# ; <# 2 2 8 1 0 % 044 0#' ! = D C #'# 5 ! 5 ; # - 0 # *8/ 9 #D ; = "5# * 0 0 ' ( % (0 # #0, + 9 "0 , - 0 #4#0 4 ;< !$ ! C #04 ( 8 # 4 . ' 0 6 &# ' 0 $ 0, # 0 #0 L "0 % # )" 0 9 " ). 0#' = $ $ D .= )# C' 0 C' 0 9 #2 !! =D# #& '' (0 -" 0 + 0, # 0 -. ) 4#) 0 & 0 # 0 L# . (0 # D# 0 ' 4 > >? ! "' #5 ' # 8 0 ( #+ 0# 6 $ 5 #0#04 # 5"0 - (5 ;#02 # 0 5 ' " # 0 4 D ; 02 " * -( /0#& '# ' 7 "04 045" % &# # ( ?. 04 7 8 ++ " # % 04 9 % + ; # . -. 0 "4 8 "0). 1 L" 0 0 & 0 (0 # D# 0 0 (0 ##0, + () #&# # ' , ) 9.#0 ' #) - 0 0 . # % # 0 <# . (0 # D# 0 9 0 0 3 ! ! 4 ; > 8".0' 6 ; 6#04 7# 1 #0 0 > 0 .# 4 #) # 0# #' #0 #) )5#04 9 # D+4 0 ' $. D₎ ₀ "5 # 0 ' 0 ) #& D# 0 ' #0 )" #0, # 0 0 ' 3 ! =% ". 0 C # 0# C % . 0 ( + ." # 1 % 5. 0 * 0 0 < . 0 6 * & 0 # 0 (0 # D# 0 () #&# + , ) #)#0 - 0 ' #0 " . 8 # 0 0 , 0 0 '# "143EE 4 ! #04 " L .+" 0 %# 0 0 + < # C - ' # 0 0 0 #,#) # 0 , () #& 9 "0 , 0 . D 0 * ) # 0 , 8 ' " # 8 0' 4 #0' (0 # D# 0 0 "0 " + () #&# + 3 %' ! " L .+" 0 %# 0 0 + < # C - (0 # D# 0 ) #&# + '' + , D ) ' 0 ) #& , ) # 0 , 5 ' " # , "# ! "'#04 6--> . . 0 4 3 4+ 5 * 5" ' * '# /0#& '# ' 1 2 .